Incidental Mutation 'R4356:Zfp352'
| ID |
327540 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp352
|
| Ensembl Gene |
ENSMUSG00000070902 |
| Gene Name |
zinc finger protein 352 |
| Synonyms |
2czf48 |
| MMRRC Submission |
041669-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R4356 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
4 |
| Chromosomal Location |
90218820-90225702 bp(+) (GRCm38) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to A
at 90223834 bp (GRCm38)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Histidine to Glutamine
at position 70
(H70Q)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000102746
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000080541]
[ENSMUST00000107129]
|
| AlphaFold |
A2AML7 |
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000080541
AA Change: H70Q
PolyPhen 2
Score 0.909 (Sensitivity: 0.81; Specificity: 0.94)
|
| SMART Domains |
Protein: ENSMUSP00000079383
Gene: ENSMUSG00000070902
AA Change: H70Q
| Domain | Start | End | E-Value | Type |
|---|
|
ZnF_C2H2
|
459 |
483 |
3.34e-2 |
SMART |
|
ZnF_C2H2
|
489 |
513 |
8.22e-2 |
SMART |
|
ZnF_C2H2
|
519 |
542 |
1.76e-1 |
SMART |
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000107129
AA Change: H70Q
PolyPhen 2
Score 0.909 (Sensitivity: 0.81; Specificity: 0.94)
|
| SMART Domains |
Protein: ENSMUSP00000102746
Gene: ENSMUSG00000070902
AA Change: H70Q
| Domain | Start | End | E-Value | Type |
|---|
|
ZnF_C2H2
|
459 |
483 |
3.34e-2 |
SMART |
|
ZnF_C2H2
|
489 |
513 |
8.22e-2 |
SMART |
|
ZnF_C2H2
|
519 |
542 |
1.76e-1 |
SMART |
|
| Meta Mutation Damage Score |
0.1795 |
| Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.6%
- 10x: 97.2%
- 20x: 95.0%
|
| Validation Efficiency |
100% (58/58) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 53 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Adam21 |
T |
C |
12: 81,558,820 (GRCm38) |
T723A |
probably damaging |
Het |
| Alox5 |
C |
T |
6: 116,420,258 (GRCm38) |
V322I |
probably benign |
Het |
| Alpk1 |
C |
T |
3: 127,729,373 (GRCm38) |
V7M |
probably damaging |
Het |
| Ankhd1 |
A |
T |
18: 36,643,043 (GRCm38) |
K1482* |
probably null |
Het |
| C130079G13Rik |
T |
A |
3: 59,936,280 (GRCm38) |
Y132N |
probably damaging |
Het |
| Cacna1e |
A |
C |
1: 154,443,981 (GRCm38) |
D1324E |
probably damaging |
Het |
| Ccdc150 |
A |
G |
1: 54,353,054 (GRCm38) |
D657G |
probably damaging |
Het |
| Celsr1 |
A |
G |
15: 85,978,827 (GRCm38) |
S1335P |
probably damaging |
Het |
| Cspg5 |
A |
G |
9: 110,256,177 (GRCm38) |
D391G |
probably damaging |
Het |
| Defa30 |
A |
T |
8: 21,134,805 (GRCm38) |
D48V |
possibly damaging |
Het |
| E2f7 |
T |
C |
10: 110,759,851 (GRCm38) |
Y136H |
probably damaging |
Het |
| Fbxw25 |
A |
T |
9: 109,662,085 (GRCm38) |
C122S |
probably damaging |
Het |
| Fgg |
A |
T |
3: 83,012,943 (GRCm38) |
D343V |
probably damaging |
Het |
| Flnb |
T |
A |
14: 7,922,700 (GRCm38) |
M1712K |
probably benign |
Het |
| Fnbp4 |
T |
C |
2: 90,758,339 (GRCm38) |
S485P |
probably damaging |
Het |
| Ftsj3 |
G |
A |
11: 106,253,676 (GRCm38) |
A134V |
probably benign |
Het |
| Gast |
C |
A |
11: 100,336,547 (GRCm38) |
S22Y |
probably damaging |
Het |
| Gm20834 |
T |
A |
Y: 10,322,962 (GRCm38) |
H158L |
possibly damaging |
Het |
| Gm4788 |
T |
A |
1: 139,732,310 (GRCm38) |
K621N |
probably damaging |
Het |
| Ids |
C |
A |
X: 70,346,344 (GRCm38) |
G506C |
probably damaging |
Het |
| Ifna15 |
G |
T |
4: 88,557,842 (GRCm38) |
T135N |
probably benign |
Het |
| Igsf9b |
G |
A |
9: 27,309,478 (GRCm38) |
V47I |
possibly damaging |
Het |
| Impg2 |
C |
T |
16: 56,260,164 (GRCm38) |
T777I |
probably damaging |
Het |
| Kif11 |
C |
A |
19: 37,411,435 (GRCm38) |
T790K |
probably benign |
Het |
| Kif24 |
A |
G |
4: 41,413,827 (GRCm38) |
|
probably null |
Het |
| Klk14 |
G |
A |
7: 43,692,077 (GRCm38) |
C51Y |
probably damaging |
Het |
| Lrp10 |
C |
T |
14: 54,468,366 (GRCm38) |
R338C |
probably damaging |
Het |
| Mib1 |
A |
G |
18: 10,751,844 (GRCm38) |
N242S |
probably benign |
Het |
| Nectin1 |
A |
G |
9: 43,792,505 (GRCm38) |
D264G |
probably benign |
Het |
| Nipsnap3a |
T |
C |
4: 52,995,979 (GRCm38) |
|
probably null |
Het |
| Oit1 |
A |
G |
14: 8,349,314 (GRCm38) |
L212P |
probably damaging |
Het |
| Olfr1137 |
G |
A |
2: 87,711,885 (GRCm38) |
S7F |
possibly damaging |
Het |
| Olfr119 |
G |
T |
17: 37,700,899 (GRCm38) |
E76D |
probably damaging |
Het |
| P3h4 |
A |
G |
11: 100,413,626 (GRCm38) |
F263S |
probably damaging |
Het |
| Pcdhga4 |
C |
T |
18: 37,687,611 (GRCm38) |
H738Y |
probably damaging |
Het |
| Prickle2 |
A |
T |
6: 92,411,509 (GRCm38) |
I304K |
probably damaging |
Het |
| Ptprq |
A |
T |
10: 107,608,364 (GRCm38) |
Y1460N |
probably damaging |
Het |
| Rbl2 |
A |
G |
8: 91,107,107 (GRCm38) |
D812G |
probably damaging |
Het |
| Rbm19 |
A |
G |
5: 120,140,362 (GRCm38) |
T737A |
possibly damaging |
Het |
| Rbsn |
G |
A |
6: 92,207,048 (GRCm38) |
L95F |
possibly damaging |
Het |
| Sall4 |
T |
C |
2: 168,755,480 (GRCm38) |
E480G |
probably benign |
Het |
| Scfd1 |
T |
A |
12: 51,439,285 (GRCm38) |
N541K |
probably benign |
Het |
| Scube3 |
G |
A |
17: 28,164,309 (GRCm38) |
G442S |
probably benign |
Het |
| Slc15a4 |
A |
G |
5: 127,604,536 (GRCm38) |
|
probably null |
Het |
| Smarcc1 |
A |
G |
9: 110,196,256 (GRCm38) |
D667G |
probably damaging |
Het |
| Sned1 |
A |
T |
1: 93,265,391 (GRCm38) |
|
probably null |
Het |
| Spsb1 |
T |
C |
4: 149,906,775 (GRCm38) |
H112R |
probably damaging |
Het |
| Sun3 |
A |
G |
11: 9,016,328 (GRCm38) |
V231A |
probably damaging |
Het |
| Vmn2r106 |
A |
T |
17: 20,279,648 (GRCm38) |
D108E |
probably benign |
Het |
| Xdh |
A |
G |
17: 73,915,690 (GRCm38) |
V560A |
probably benign |
Het |
| Zfp40 |
C |
A |
17: 23,177,190 (GRCm38) |
C73F |
probably benign |
Het |
| Zfp975 |
A |
C |
7: 42,661,827 (GRCm38) |
L454R |
probably damaging |
Het |
| Zik1 |
A |
T |
7: 10,490,341 (GRCm38) |
C276* |
probably null |
Het |
|
| Other mutations in Zfp352 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01963:Zfp352
|
APN |
4 |
90,224,154 (GRCm38) |
missense |
possibly damaging |
0.95 |
|
IGL02252:Zfp352
|
APN |
4 |
90,224,130 (GRCm38) |
missense |
probably benign |
0.02 |
|
IGL03156:Zfp352
|
APN |
4 |
90,224,087 (GRCm38) |
missense |
possibly damaging |
0.57 |
|
IGL03167:Zfp352
|
APN |
4 |
90,224,702 (GRCm38) |
missense |
probably damaging |
0.99 |
|
IGL03190:Zfp352
|
APN |
4 |
90,223,757 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
IGL03335:Zfp352
|
APN |
4 |
90,224,346 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R0051:Zfp352
|
UTSW |
4 |
90,224,285 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R0403:Zfp352
|
UTSW |
4 |
90,225,009 (GRCm38) |
missense |
possibly damaging |
0.60 |
|
R0550:Zfp352
|
UTSW |
4 |
90,224,690 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R0671:Zfp352
|
UTSW |
4 |
90,223,919 (GRCm38) |
missense |
probably benign |
|
|
R1034:Zfp352
|
UTSW |
4 |
90,224,156 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R1754:Zfp352
|
UTSW |
4 |
90,223,809 (GRCm38) |
missense |
probably benign |
0.23 |
|
R2016:Zfp352
|
UTSW |
4 |
90,225,171 (GRCm38) |
missense |
probably benign |
0.42 |
|
R2064:Zfp352
|
UTSW |
4 |
90,225,120 (GRCm38) |
missense |
probably benign |
0.08 |
|
R2308:Zfp352
|
UTSW |
4 |
90,225,243 (GRCm38) |
missense |
probably benign |
0.00 |
|
R3552:Zfp352
|
UTSW |
4 |
90,225,102 (GRCm38) |
missense |
probably benign |
0.33 |
|
R3794:Zfp352
|
UTSW |
4 |
90,225,149 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R3795:Zfp352
|
UTSW |
4 |
90,225,149 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R4135:Zfp352
|
UTSW |
4 |
90,225,024 (GRCm38) |
missense |
probably damaging |
0.96 |
|
R4409:Zfp352
|
UTSW |
4 |
90,225,164 (GRCm38) |
missense |
probably benign |
0.00 |
|
R4590:Zfp352
|
UTSW |
4 |
90,224,535 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R4614:Zfp352
|
UTSW |
4 |
90,225,081 (GRCm38) |
missense |
probably benign |
0.00 |
|
R4617:Zfp352
|
UTSW |
4 |
90,225,081 (GRCm38) |
missense |
probably benign |
0.00 |
|
R4618:Zfp352
|
UTSW |
4 |
90,225,081 (GRCm38) |
missense |
probably benign |
0.00 |
|
R4741:Zfp352
|
UTSW |
4 |
90,224,940 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R4931:Zfp352
|
UTSW |
4 |
90,224,304 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R4959:Zfp352
|
UTSW |
4 |
90,224,139 (GRCm38) |
missense |
probably benign |
0.01 |
|
R4973:Zfp352
|
UTSW |
4 |
90,224,139 (GRCm38) |
missense |
probably benign |
0.01 |
|
R5167:Zfp352
|
UTSW |
4 |
90,224,216 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R5260:Zfp352
|
UTSW |
4 |
90,224,460 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R5524:Zfp352
|
UTSW |
4 |
90,225,104 (GRCm38) |
missense |
possibly damaging |
0.95 |
|
R5942:Zfp352
|
UTSW |
4 |
90,225,070 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R6802:Zfp352
|
UTSW |
4 |
90,225,200 (GRCm38) |
missense |
probably benign |
0.33 |
|
R6819:Zfp352
|
UTSW |
4 |
90,224,699 (GRCm38) |
missense |
probably benign |
|
|
R7072:Zfp352
|
UTSW |
4 |
90,224,424 (GRCm38) |
missense |
probably benign |
0.00 |
|
R7099:Zfp352
|
UTSW |
4 |
90,224,880 (GRCm38) |
missense |
probably benign |
0.00 |
|
R7569:Zfp352
|
UTSW |
4 |
90,223,659 (GRCm38) |
missense |
possibly damaging |
0.77 |
|
R7645:Zfp352
|
UTSW |
4 |
90,224,777 (GRCm38) |
missense |
probably benign |
0.13 |
|
R7705:Zfp352
|
UTSW |
4 |
90,225,275 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R8424:Zfp352
|
UTSW |
4 |
90,224,243 (GRCm38) |
missense |
possibly damaging |
0.87 |
|
R9180:Zfp352
|
UTSW |
4 |
90,224,881 (GRCm38) |
missense |
probably benign |
0.38 |
|
R9378:Zfp352
|
UTSW |
4 |
90,224,338 (GRCm38) |
missense |
probably benign |
0.13 |
|
R9509:Zfp352
|
UTSW |
4 |
90,224,706 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R9623:Zfp352
|
UTSW |
4 |
90,224,891 (GRCm38) |
missense |
probably benign |
0.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- AAAACACAGGTGCTTCCCAG -3'
(R):5'- AGTCTTGTCAGAGTTCAGTGTCC -3'
Sequencing Primer
(F):5'- TGCTTCCCAGCCAGGAC -3'
(R):5'- AGAGTTCAGTGTCCTCATCCAGTAG -3'
|
| Posted On |
2015-07-07 |
Incidental Mutation