Incidental Mutation 'R4356:Oit1'
ID327566
Institutional Source Beutler Lab
Gene Symbol Oit1
Ensembl Gene ENSMUSG00000021749
Gene Nameoncoprotein induced transcript 1
SynonymsEF-7, Fam3d, 2310076N21Rik
MMRRC Submission 041669-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.053) question?
Stock #R4356 (G1)
Quality Score225
Status Validated
Chromosome14
Chromosomal Location8348937-8378754 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to G at 8349314 bp
ZygosityHeterozygous
Amino Acid Change Leucine to Proline at position 212 (L212P)
Ref Sequence ENSEMBL: ENSMUSP00000022269 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000022269]
Predicted Effect probably damaging
Transcript: ENSMUST00000022269
AA Change: L212P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000022269
Gene: ENSMUSG00000021749
AA Change: L212P

DomainStartEndE-ValueType
transmembrane domain 4 26 N/A INTRINSIC
Pfam:ILEI 98 190 3.1e-31 PFAM
Meta Mutation Damage Score 0.2321 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.2%
  • 20x: 95.0%
Validation Efficiency 100% (58/58)
MGI Phenotype PHENOTYPE: Male mice homozygous for a targeted mutation exhibit emotion/affect abnormalities including decreased anxiety and depression responses. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 53 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adam21 T C 12: 81,558,820 T723A probably damaging Het
Alox5 C T 6: 116,420,258 V322I probably benign Het
Alpk1 C T 3: 127,729,373 V7M probably damaging Het
Ankhd1 A T 18: 36,643,043 K1482* probably null Het
C130079G13Rik T A 3: 59,936,280 Y132N probably damaging Het
Cacna1e A C 1: 154,443,981 D1324E probably damaging Het
Ccdc150 A G 1: 54,353,054 D657G probably damaging Het
Celsr1 A G 15: 85,978,827 S1335P probably damaging Het
Cspg5 A G 9: 110,256,177 D391G probably damaging Het
Defa30 A T 8: 21,134,805 D48V possibly damaging Het
E2f7 T C 10: 110,759,851 Y136H probably damaging Het
Fbxw25 A T 9: 109,662,085 C122S probably damaging Het
Fgg A T 3: 83,012,943 D343V probably damaging Het
Flnb T A 14: 7,922,700 M1712K probably benign Het
Fnbp4 T C 2: 90,758,339 S485P probably damaging Het
Ftsj3 G A 11: 106,253,676 A134V probably benign Het
Gast C A 11: 100,336,547 S22Y probably damaging Het
Gm20834 T A Y: 10,322,962 H158L possibly damaging Het
Gm4788 T A 1: 139,732,310 K621N probably damaging Het
Ids C A X: 70,346,344 G506C probably damaging Het
Ifna15 G T 4: 88,557,842 T135N probably benign Het
Igsf9b G A 9: 27,309,478 V47I possibly damaging Het
Impg2 C T 16: 56,260,164 T777I probably damaging Het
Kif11 C A 19: 37,411,435 T790K probably benign Het
Kif24 A G 4: 41,413,827 probably null Het
Klk14 G A 7: 43,692,077 C51Y probably damaging Het
Lrp10 C T 14: 54,468,366 R338C probably damaging Het
Mib1 A G 18: 10,751,844 N242S probably benign Het
Nectin1 A G 9: 43,792,505 D264G probably benign Het
Nipsnap3a T C 4: 52,995,979 probably null Het
Olfr1137 G A 2: 87,711,885 S7F possibly damaging Het
Olfr119 G T 17: 37,700,899 E76D probably damaging Het
P3h4 A G 11: 100,413,626 F263S probably damaging Het
Pcdhga4 C T 18: 37,687,611 H738Y probably damaging Het
Prickle2 A T 6: 92,411,509 I304K probably damaging Het
Ptprq A T 10: 107,608,364 Y1460N probably damaging Het
Rbl2 A G 8: 91,107,107 D812G probably damaging Het
Rbm19 A G 5: 120,140,362 T737A possibly damaging Het
Rbsn G A 6: 92,207,048 L95F possibly damaging Het
Sall4 T C 2: 168,755,480 E480G probably benign Het
Scfd1 T A 12: 51,439,285 N541K probably benign Het
Scube3 G A 17: 28,164,309 G442S probably benign Het
Slc15a4 A G 5: 127,604,536 probably null Het
Smarcc1 A G 9: 110,196,256 D667G probably damaging Het
Sned1 A T 1: 93,265,391 probably null Het
Spsb1 T C 4: 149,906,775 H112R probably damaging Het
Sun3 A G 11: 9,016,328 V231A probably damaging Het
Vmn2r106 A T 17: 20,279,648 D108E probably benign Het
Xdh A G 17: 73,915,690 V560A probably benign Het
Zfp352 T A 4: 90,223,834 H70Q possibly damaging Het
Zfp40 C A 17: 23,177,190 C73F probably benign Het
Zfp975 A C 7: 42,661,827 L454R probably damaging Het
Zik1 A T 7: 10,490,341 C276* probably null Het
Other mutations in Oit1
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02795:Oit1 APN 14 8355497 missense probably benign
IGL02850:Oit1 APN 14 8361475 critical splice donor site probably null
R0899:Oit1 UTSW 14 8364863 missense probably damaging 0.99
R2098:Oit1 UTSW 14 8361479 missense probably benign 0.14
R4304:Oit1 UTSW 14 8349324 missense probably damaging 1.00
R4603:Oit1 UTSW 14 8358429 missense probably damaging 0.99
R4705:Oit1 UTSW 14 8349347 missense probably benign 0.01
R5650:Oit1 UTSW 14 8357142 missense probably damaging 0.99
R5679:Oit1 UTSW 14 8349305 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- AGTGAGTCACTGTGTGCAGG -3'
(R):5'- CTGAGACCCAGAGCAAGTTG -3'

Sequencing Primer
(F):5'- CACTGTGTGCAGGGGATGATC -3'
(R):5'- TGGCCCCATCTGTAAAATGG -3'
Posted On2015-07-07