Mutagenetix > Incidental Mutations

Incidental Mutation 'R4414:Try4'

328060

Institutional Source

Beutler Lab

Gene Symbol

Try4

Ensembl Gene

ENSMUSG00000054106

Gene Name

trypsin 4

Synonyms

0910001B19Rik, Td

MMRRC Submission

041694-MU

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4414 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

41302269-41305532 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 41304971 bp

Zygosity

Heterozygous

Amino Acid Change

Proline to Serine at position 164 (P164S)

Ref Sequence

ENSEMBL: ENSMUSP00000031913 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000031913]

Predicted Effect

possibly damaging
Transcript: ENSMUST00000031913
AA Change: P164S

PolyPhen 2 Score 0.838 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000031913
Gene: ENSMUSG00000054106
AA Change: P164S

Domain	Start	End	E-Value	Type
low complexity region	3	16	N/A	INTRINSIC
Tryp_SPc	23	239	9.72e-105	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000193013

Meta Mutation Damage Score

0.3688

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.2%
20x: 95.0%

Validation Efficiency

100% (50/50)

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110017D15Rik	T	C	4: 41,505,574	T183A	possibly damaging	Het
4930503E14Rik	T	A	14: 44,169,233	M120L	probably benign	Het
Aco2	A	G	15: 81,889,383		probably null	Het
Acss1	A	G	2: 150,659,903	S115P	possibly damaging	Het
Ank2	A	T	3: 127,225,762		probably null	Het
AU041133	C	T	10: 82,151,482	T323M	probably damaging	Het
Bdp1	T	C	13: 100,030,861	D2215G	probably damaging	Het
Bod1l	G	A	5: 41,820,527	T1148I	probably benign	Het
Celsr1	C	T	15: 85,963,133	V1468I	probably benign	Het
Celsr1	A	G	15: 85,927,999	V2065A	probably damaging	Het
Cep89	A	G	7: 35,416,397		probably benign	Het
Cfap58	A	G	19: 47,953,041	K283E	possibly damaging	Het
Cog5	C	T	12: 31,660,854	Q78*	probably null	Het
Col20a1	C	T	2: 181,001,250	R796C	possibly damaging	Het
Dnah7b	A	T	1: 46,126,680	T502S	probably benign	Het
Dnm1l	A	G	16: 16,342,695		probably null	Het
Dse	A	T	10: 34,152,636	F819L	probably benign	Het
Eloa	G	T	4: 136,011,242	L136I	possibly damaging	Het
Eloa	T	A	4: 136,011,265	H128L	probably benign	Het
Fbxl6	T	C	15: 76,537,724	E205G	possibly damaging	Het
Golim4	T	G	3: 75,895,040	N287T	probably benign	Het
Gpx8	T	A	13: 113,043,148	K206N	possibly damaging	Het
Iqgap3	G	T	3: 88,096,986	V460F	probably benign	Het
Kcne4	A	T	1: 78,817,934	M100L	probably benign	Het
Kmt2a	A	T	9: 44,809,780		probably benign	Het
Ktn1	G	A	14: 47,724,930	W1117*	probably null	Het
Lad1	A	G	1: 135,828,746	D364G	probably benign	Het
Lmln	T	G	16: 33,109,850	I559S	probably benign	Het
Mapk4	A	T	18: 73,930,538	F538I	possibly damaging	Het
Mlxipl	A	G	5: 135,137,399		probably benign	Het
Mmrn1	T	C	6: 60,944,586	L9P	probably damaging	Het
Mnat1	G	A	12: 73,181,827	R155H	probably damaging	Het
Mtmr11	T	C	3: 96,167,891		probably benign	Het
Naaladl1	T	C	19: 6,115,551	L745P	probably damaging	Het
Obsl1	A	T	1: 75,490,902	D1409E	probably benign	Het
Oit3	T	C	10: 59,428,103	Y403C	probably damaging	Het
Pla2g4e	A	T	2: 120,182,713	H375Q	probably benign	Het
Prodh2	G	A	7: 30,506,452	V238M	probably damaging	Het
Rdh14	G	A	12: 10,391,231		probably null	Het
Rho	G	A	6: 115,935,230	V76I	probably benign	Het
Rock1	A	T	18: 10,080,514	M1010K	probably damaging	Het
Ros1	A	G	10: 52,162,704		probably null	Het
Sim1	A	T	10: 50,981,612	D486V	probably benign	Het
Src	A	G	2: 157,464,653	D192G	probably damaging	Het
Stox1	T	C	10: 62,659,569	N975S	probably benign	Het
Tmem145	A	G	7: 25,307,129	Y54C	probably damaging	Het
Vegfc	A	T	8: 54,181,095	N270Y	probably benign	Het

Other mutations in Try4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01773:Try4	APN	6	41305026	missense	probably damaging	1.00
IGL02216:Try4	APN	6	41305031	missense	probably benign
R0537:Try4	UTSW	6	41304362	missense	probably benign
R0731:Try4	UTSW	6	41304367	missense	probably benign	0.01
R1113:Try4	UTSW	6	41305374	missense	possibly damaging	0.90
R1833:Try4	UTSW	6	41303431	missense	probably damaging	0.98
R2246:Try4	UTSW	6	41305472	missense	possibly damaging	0.80
R4131:Try4	UTSW	6	41305401	nonsense	probably null
R5457:Try4	UTSW	6	41303421	missense	probably damaging	1.00
R5707:Try4	UTSW	6	41305043	missense	possibly damaging	0.65
R6023:Try4	UTSW	6	41303421	missense	probably damaging	1.00
R7131:Try4	UTSW	6	41304403	missense	probably benign	0.03
R7783:Try4	UTSW	6	41302295	missense	possibly damaging	0.96
RF007:Try4	UTSW	6	41305363	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CATGTAAACAGAGCAGCATGTAAAC -3'
(R):5'- CCTCATACACTTGGGCATGC -3'

Sequencing Primer
(F):5'- CTGTTATCACTTCTAGGAAGGACATC -3'
(R):5'- TACACTTGGGCATGCTAACC -3'

Posted On

2015-07-07