Mutagenetix > Incidental Mutation

Incidental Mutation 'R4427:Vmn2r73'

328229

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r73

Ensembl Gene

ENSMUSG00000070458

Gene Name

vomeronasal 2, receptor 73

Synonyms

EG620928

MMRRC Submission

041145-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.172) question?

Stock #

R4427 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

85506755-85525146 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 85506981 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Serine at position 777 (F777S)

Ref Sequence

ENSEMBL: ENSMUSP00000076687 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077478]

AlphaFold

D3Z7M3

Predicted Effect

probably damaging
Transcript: ENSMUST00000077478
AA Change: F777S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000076687
Gene: ENSMUSG00000070458
AA Change: F777S

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	465	1.4e-30	PFAM
Pfam:NCD3G	508	560	5.9e-21	PFAM
Pfam:7tm_3	590	827	4.4e-51	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 95.0%

Validation Efficiency

96% (48/50)

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930432E11Rik	A	T	7: 29,278,678 (GRCm39)		noncoding transcript	Het
A2ml1	T	C	6: 128,522,009 (GRCm39)	E1271G	probably benign	Het
Ccdc88b	C	T	19: 6,827,940 (GRCm39)	E878K	probably damaging	Het
Cfap119	A	G	7: 127,187,288 (GRCm39)		probably benign	Het
Crybg3	T	C	16: 59,363,562 (GRCm39)	K2441E	probably damaging	Het
Cryga	A	T	1: 65,139,775 (GRCm39)	I121N	probably damaging	Het
Dst	A	T	1: 34,220,541 (GRCm39)	Q2115L	probably benign	Het
Evi2	T	A	11: 79,407,182 (GRCm39)	Q131L	possibly damaging	Het
Exoc1	A	G	5: 76,711,110 (GRCm39)	I61V	probably benign	Het
Firrm	A	G	1: 163,781,853 (GRCm39)	V858A	probably benign	Het
Frem2	A	G	3: 53,446,583 (GRCm39)		probably null	Het
Gas2l1	A	G	11: 5,013,908 (GRCm39)	V184A	probably benign	Het
Gsto2	T	C	19: 47,860,212 (GRCm39)	S2P	possibly damaging	Het
Herc1	T	C	9: 66,403,287 (GRCm39)	L4402P	probably damaging	Het
Kcnd2	T	A	6: 21,216,896 (GRCm39)	I200N	probably damaging	Het
Klhl30	A	G	1: 91,281,426 (GRCm39)	D9G	probably damaging	Het
Ltf	C	A	9: 110,852,672 (GRCm39)	T178K	probably damaging	Het
Memo1	T	A	17: 74,509,302 (GRCm39)	Y239F	probably benign	Het
Ogdh	C	T	11: 6,305,421 (GRCm39)	T972I	probably benign	Het
Phactr4	T	C	4: 132,114,352 (GRCm39)	D24G	possibly damaging	Het
Pi4ka	C	T	16: 17,098,908 (GRCm39)	R1992H	probably damaging	Het
Poc1b	T	C	10: 98,991,001 (GRCm39)		probably null	Het
Ppp1r9b	A	T	11: 94,892,150 (GRCm39)	R188S	possibly damaging	Het
Pwwp2a	T	C	11: 43,573,344 (GRCm39)	V142A	possibly damaging	Het
Rab36	G	A	10: 74,880,328 (GRCm39)	V63I	probably damaging	Het
Rap1gap2	C	A	11: 74,298,148 (GRCm39)	A491S	possibly damaging	Het
Rcsd1	C	A	1: 165,483,464 (GRCm39)	V206L	probably damaging	Het
Rps6ka2	T	A	17: 7,566,804 (GRCm39)	D687E	possibly damaging	Het
Sgce	G	A	6: 4,691,459 (GRCm39)	A295V	probably damaging	Het
Siglec15	T	G	18: 78,086,836 (GRCm39)	E341A	possibly damaging	Het
Tcaim	T	C	9: 122,643,561 (GRCm39)	F87S	probably benign	Het
Thbs2	C	A	17: 14,900,597 (GRCm39)	V537L	probably benign	Het
Timd6	T	A	11: 46,475,247 (GRCm39)	F147L	probably benign	Het
Tmx3	T	A	18: 90,541,725 (GRCm39)	V158D	probably damaging	Het
Tpm1	A	G	9: 66,939,847 (GRCm39)		probably benign	Het
Trmt2a	A	G	16: 18,067,093 (GRCm39)		probably benign	Het
Ugcg	T	A	4: 59,219,555 (GRCm39)	F297L	probably benign	Het
Utp18	T	C	11: 93,757,264 (GRCm39)	N467D	probably damaging	Het
Vwc2	A	G	11: 11,104,235 (GRCm39)	T256A	probably damaging	Het
Zfp300	C	T	X: 20,949,405 (GRCm39)	V120I	possibly damaging	Het
Zfp982	A	T	4: 147,597,080 (GRCm39)	R146*	probably null	Het

Other mutations in Vmn2r73

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00158:Vmn2r73	APN	7	85,506,795 (GRCm39)	missense
IGL01151:Vmn2r73	APN	7	85,507,086 (GRCm39)	missense	probably damaging	0.99
IGL01408:Vmn2r73	APN	7	85,521,455 (GRCm39)	missense	probably benign	0.45
IGL01505:Vmn2r73	APN	7	85,507,267 (GRCm39)	nonsense	probably null
IGL01731:Vmn2r73	APN	7	85,506,757 (GRCm39)	makesense	probably null
IGL01818:Vmn2r73	APN	7	85,519,109 (GRCm39)	splice site	probably benign
IGL02236:Vmn2r73	APN	7	85,521,902 (GRCm39)	missense	probably benign	0.00
IGL02265:Vmn2r73	APN	7	85,520,847 (GRCm39)	missense	probably benign	0.02
IGL02266:Vmn2r73	APN	7	85,525,007 (GRCm39)	missense	possibly damaging	0.52
IGL02276:Vmn2r73	APN	7	85,518,980 (GRCm39)	critical splice donor site	probably null
IGL02284:Vmn2r73	APN	7	85,507,123 (GRCm39)	missense	possibly damaging	0.92
IGL02380:Vmn2r73	APN	7	85,507,383 (GRCm39)	missense	probably benign	0.00
IGL02466:Vmn2r73	APN	7	85,522,084 (GRCm39)	missense	probably damaging	1.00
IGL03172:Vmn2r73	APN	7	85,507,495 (GRCm39)	missense	probably benign	0.01
IGL03179:Vmn2r73	APN	7	85,519,468 (GRCm39)	missense	probably benign	0.04
G1Funyon:Vmn2r73	UTSW	7	85,507,510 (GRCm39)	missense	probably benign	0.00
R0077:Vmn2r73	UTSW	7	85,525,075 (GRCm39)	missense	probably benign	0.00
R0311:Vmn2r73	UTSW	7	85,520,997 (GRCm39)	missense	probably benign
R0413:Vmn2r73	UTSW	7	85,521,087 (GRCm39)	missense	possibly damaging	0.68
R0494:Vmn2r73	UTSW	7	85,522,140 (GRCm39)	missense	probably benign
R1523:Vmn2r73	UTSW	7	85,519,486 (GRCm39)	missense	probably benign	0.02
R1618:Vmn2r73	UTSW	7	85,525,120 (GRCm39)	nonsense	probably null
R1667:Vmn2r73	UTSW	7	85,506,889 (GRCm39)	missense	probably benign	0.00
R1728:Vmn2r73	UTSW	7	85,507,086 (GRCm39)	missense	probably damaging	1.00
R1729:Vmn2r73	UTSW	7	85,507,086 (GRCm39)	missense	probably damaging	1.00
R1747:Vmn2r73	UTSW	7	85,507,375 (GRCm39)	missense	probably damaging	1.00
R1784:Vmn2r73	UTSW	7	85,507,086 (GRCm39)	missense	probably damaging	1.00
R2152:Vmn2r73	UTSW	7	85,506,936 (GRCm39)	missense	probably benign	0.03
R2395:Vmn2r73	UTSW	7	85,506,975 (GRCm39)	missense	probably damaging	1.00
R2415:Vmn2r73	UTSW	7	85,521,431 (GRCm39)	missense	probably damaging	1.00
R2926:Vmn2r73	UTSW	7	85,520,871 (GRCm39)	missense	probably benign	0.17
R3719:Vmn2r73	UTSW	7	85,519,582 (GRCm39)	missense	probably damaging	1.00
R3766:Vmn2r73	UTSW	7	85,521,198 (GRCm39)	missense	probably benign
R3838:Vmn2r73	UTSW	7	85,507,258 (GRCm39)	missense	probably benign	0.00
R3890:Vmn2r73	UTSW	7	85,507,144 (GRCm39)	missense	probably benign
R4030:Vmn2r73	UTSW	7	85,521,044 (GRCm39)	missense	possibly damaging	0.92
R4247:Vmn2r73	UTSW	7	85,521,883 (GRCm39)	missense	probably damaging	0.99
R4409:Vmn2r73	UTSW	7	85,520,768 (GRCm39)	missense	probably damaging	0.97
R4430:Vmn2r73	UTSW	7	85,519,449 (GRCm39)	missense	probably benign
R4552:Vmn2r73	UTSW	7	85,525,055 (GRCm39)	missense	probably benign	0.12
R4779:Vmn2r73	UTSW	7	85,520,923 (GRCm39)	nonsense	probably null
R4906:Vmn2r73	UTSW	7	85,506,829 (GRCm39)	missense	probably damaging	0.99
R4942:Vmn2r73	UTSW	7	85,519,582 (GRCm39)	missense	probably damaging	1.00
R5280:Vmn2r73	UTSW	7	85,507,155 (GRCm39)	missense	probably damaging	0.98
R5344:Vmn2r73	UTSW	7	85,525,046 (GRCm39)	missense	probably benign	0.00
R5478:Vmn2r73	UTSW	7	85,518,996 (GRCm39)	missense	probably damaging	1.00
R5527:Vmn2r73	UTSW	7	85,507,575 (GRCm39)	missense	possibly damaging	0.65
R5691:Vmn2r73	UTSW	7	85,507,299 (GRCm39)	missense	probably damaging	1.00
R5826:Vmn2r73	UTSW	7	85,524,956 (GRCm39)	missense	possibly damaging	0.77
R6018:Vmn2r73	UTSW	7	85,521,875 (GRCm39)	missense	possibly damaging	0.48
R6082:Vmn2r73	UTSW	7	85,507,429 (GRCm39)	missense	probably benign	0.00
R6111:Vmn2r73	UTSW	7	85,520,997 (GRCm39)	missense	probably benign
R6233:Vmn2r73	UTSW	7	85,519,099 (GRCm39)	missense	probably benign	0.00
R6278:Vmn2r73	UTSW	7	85,522,140 (GRCm39)	missense	probably benign
R6283:Vmn2r73	UTSW	7	85,521,049 (GRCm39)	missense	probably benign	0.00
R6307:Vmn2r73	UTSW	7	85,506,828 (GRCm39)	missense	probably damaging	1.00
R6413:Vmn2r73	UTSW	7	85,519,544 (GRCm39)	nonsense	probably null
R6782:Vmn2r73	UTSW	7	85,519,563 (GRCm39)	missense	probably benign	0.00
R6884:Vmn2r73	UTSW	7	85,507,213 (GRCm39)	missense	probably benign	0.05
R6921:Vmn2r73	UTSW	7	85,507,446 (GRCm39)	missense	probably benign	0.27
R7169:Vmn2r73	UTSW	7	85,507,663 (GRCm39)	nonsense	probably null
R7206:Vmn2r73	UTSW	7	85,522,075 (GRCm39)	missense	probably benign	0.03
R7311:Vmn2r73	UTSW	7	85,521,192 (GRCm39)	missense	possibly damaging	0.80
R7650:Vmn2r73	UTSW	7	85,521,147 (GRCm39)	missense	probably benign
R7811:Vmn2r73	UTSW	7	85,524,956 (GRCm39)	missense	possibly damaging	0.77
R8263:Vmn2r73	UTSW	7	85,507,619 (GRCm39)	missense	probably benign
R8301:Vmn2r73	UTSW	7	85,507,510 (GRCm39)	missense	probably benign	0.00
R8341:Vmn2r73	UTSW	7	85,507,128 (GRCm39)	missense	probably benign	0.01
R8500:Vmn2r73	UTSW	7	85,519,638 (GRCm39)	missense	probably benign	0.01
R8673:Vmn2r73	UTSW	7	85,521,902 (GRCm39)	missense	probably benign	0.01
R8719:Vmn2r73	UTSW	7	85,521,095 (GRCm39)	missense	probably damaging	0.96
R8783:Vmn2r73	UTSW	7	85,507,668 (GRCm39)	missense	probably damaging	0.96
R8828:Vmn2r73	UTSW	7	85,521,179 (GRCm39)	missense	probably damaging	0.98
R8860:Vmn2r73	UTSW	7	85,522,149 (GRCm39)	splice site	probably benign
R9002:Vmn2r73	UTSW	7	85,507,284 (GRCm39)	missense	probably benign	0.32
R9008:Vmn2r73	UTSW	7	85,521,904 (GRCm39)	missense	probably damaging	0.96
R9159:Vmn2r73	UTSW	7	85,521,931 (GRCm39)	missense	possibly damaging	0.90
R9180:Vmn2r73	UTSW	7	85,507,123 (GRCm39)	missense	probably benign	0.07
R9198:Vmn2r73	UTSW	7	85,518,987 (GRCm39)	missense	probably benign	0.01
R9329:Vmn2r73	UTSW	7	85,519,073 (GRCm39)	missense	probably benign
R9448:Vmn2r73	UTSW	7	85,522,027 (GRCm39)	missense	probably benign	0.00
R9463:Vmn2r73	UTSW	7	85,506,795 (GRCm39)	missense
R9589:Vmn2r73	UTSW	7	85,519,659 (GRCm39)	missense	probably benign	0.03
X0023:Vmn2r73	UTSW	7	85,507,664 (GRCm39)	missense	probably benign	0.31
Z1176:Vmn2r73	UTSW	7	85,521,176 (GRCm39)	missense	probably damaging	0.99
Z1177:Vmn2r73	UTSW	7	85,521,480 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGATGAGTTGAAAGCAGCTTTG -3'
(R):5'- TGATGCTGATCTACACATGGTAC -3'

Sequencing Primer
(F):5'- CATATGAGAAGAATTTTTCCCTGGTC -3'
(R):5'- GGTACATGGCCACATCATCATTG -3'

Posted On

2015-07-07