Mutagenetix > Incidental Mutations

Incidental Mutation 'R4428:Vmn2r22'

328273

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r22

Ensembl Gene

ENSMUSG00000095486

Gene Name

vomeronasal 2, receptor 22

Synonyms

EG546913

MMRRC Submission

041698-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.062) question?

Stock #

R4428 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

123609758-123650635 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 123637858 bp

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 258 (T258A)

Ref Sequence

ENSEMBL: ENSMUSP00000132043 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170808]

Predicted Effect

possibly damaging
Transcript: ENSMUST00000170808
AA Change: T258A

PolyPhen 2 Score 0.468 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000132043
Gene: ENSMUSG00000095486
AA Change: T258A

Domain	Start	End	E-Value	Type
signal peptide	1	17	N/A	INTRINSIC
Pfam:ANF_receptor	81	473	1.4e-32	PFAM
Pfam:NCD3G	517	570	2.2e-23	PFAM
Pfam:7tm_3	601	838	1.2e-54	PFAM

Meta Mutation Damage Score

0.0596

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.5%

Validation Efficiency

100% (46/46)

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610040J01Rik	G	T	5: 63,898,839		probably benign	Het
Abcc1	T	A	16: 14,445,300	V708E	probably damaging	Het
Bag4	C	T	8: 25,769,488	A228T	probably benign	Het
Ccdc18	A	G	5: 108,136,077	Y82C	probably benign	Het
Cd82	T	C	2: 93,419,869	Y266C	probably damaging	Het
Chrna4	A	G	2: 181,028,620	S448P	probably damaging	Het
Cldn8	A	C	16: 88,562,731	M102R	probably damaging	Het
Cmpk1	T	C	4: 114,963,362	E180G	probably benign	Het
Dock8	T	C	19: 25,200,499	I2066T	probably damaging	Het
Dock8	T	C	19: 25,065,390	V112A	probably benign	Het
Fgf3	T	C	7: 144,840,707	V86A	probably damaging	Het
Frem2	A	G	3: 53,654,338	I916T	probably benign	Het
Gsk3b	T	A	16: 38,193,936	L252Q	probably damaging	Het
Kif18a	A	G	2: 109,288,121	T94A	probably damaging	Het
Klhdc1	T	A	12: 69,268,226		probably benign	Het
Klhl25	T	A	7: 75,865,414	F23I	probably damaging	Het
Mapk7	T	C	11: 61,489,229	D701G	possibly damaging	Het
Mbd5	A	T	2: 49,279,764	Q47L	possibly damaging	Het
Nrcam	A	G	12: 44,576,775	D1049G	possibly damaging	Het
Olfml2a	G	T	2: 38,941,743	M111I	probably damaging	Het
Olfr402	T	C	11: 74,155,199	F15S	probably damaging	Het
Pld2	A	T	11: 70,541,334	H93L	probably damaging	Het
Pomgnt2	T	C	9: 121,982,254	E487G	possibly damaging	Het
Psg17	G	T	7: 18,816,792	N379K	probably benign	Het
Rfk	T	A	19: 17,398,595	H84Q	possibly damaging	Het
Scn8a	A	G	15: 100,983,903	Y617C	probably damaging	Het
Sema3d	T	C	5: 12,448,120	F31S	probably benign	Het
Shq1	T	C	6: 100,670,928	Y45C	probably damaging	Het
Siglecg	C	T	7: 43,417,926	P639L	possibly damaging	Het
Skp2	A	T	15: 9,116,947	N325K	probably benign	Het
Sost	G	A	11: 101,966,844	P44S	probably damaging	Het
Sp8	T	A	12: 118,849,203	S264R	possibly damaging	Het
Sun1	T	C	5: 139,234,475		probably benign	Het
Tardbp	A	G	4: 148,625,202	V54A	possibly damaging	Het
Tert	T	A	13: 73,627,475	F115Y	probably damaging	Het
Tmem181a	T	A	17: 6,295,786	L185H	probably damaging	Het
Tmem68	A	T	4: 3,569,534	I52K	probably benign	Het
Trhde	A	T	10: 114,503,123	L594Q	probably damaging	Het
Umps	A	C	16: 33,961,586	V322G	probably damaging	Het
Vmn2r109	T	C	17: 20,553,024	D445G	probably benign	Het
Vmn2r4	C	T	3: 64,415,169	G43E	probably damaging	Het

Other mutations in Vmn2r22

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01024:Vmn2r22	APN	6	123638053	missense	probably damaging	1.00
IGL01764:Vmn2r22	APN	6	123650420	critical splice donor site	probably null
IGL02037:Vmn2r22	APN	6	123649067	missense	probably damaging	1.00
IGL02183:Vmn2r22	APN	6	123638004	missense	probably damaging	1.00
IGL02335:Vmn2r22	APN	6	123638092	missense	probably damaging	0.99
IGL02440:Vmn2r22	APN	6	123637405	missense	probably benign	0.00
IGL02663:Vmn2r22	APN	6	123649158	missense	probably benign	0.11
IGL03101:Vmn2r22	APN	6	123637336	missense	probably benign	0.09
R0266:Vmn2r22	UTSW	6	123637404	missense	probably damaging	0.99
R0348:Vmn2r22	UTSW	6	123637725	missense	probably damaging	1.00
R0780:Vmn2r22	UTSW	6	123637974	missense	probably damaging	1.00
R0849:Vmn2r22	UTSW	6	123637404	missense	probably damaging	0.99
R1074:Vmn2r22	UTSW	6	123649258	missense	probably benign	0.02
R1456:Vmn2r22	UTSW	6	123637665	missense	possibly damaging	0.86
R1719:Vmn2r22	UTSW	6	123637843	missense	possibly damaging	0.68
R1989:Vmn2r22	UTSW	6	123637541	missense	probably damaging	1.00
R2928:Vmn2r22	UTSW	6	123637443	missense	probably damaging	0.96
R2939:Vmn2r22	UTSW	6	123637635	missense	probably damaging	0.99
R3727:Vmn2r22	UTSW	6	123650625	missense	possibly damaging	0.70
R3782:Vmn2r22	UTSW	6	123650632	nonsense	probably null
R3873:Vmn2r22	UTSW	6	123637380	missense	possibly damaging	0.68
R4344:Vmn2r22	UTSW	6	123637797	missense	probably damaging	1.00
R4407:Vmn2r22	UTSW	6	123637954	missense	probably damaging	1.00
R4430:Vmn2r22	UTSW	6	123637858	missense	possibly damaging	0.47
R4431:Vmn2r22	UTSW	6	123637858	missense	possibly damaging	0.47
R4701:Vmn2r22	UTSW	6	123650469	missense	probably benign	0.00
R5274:Vmn2r22	UTSW	6	123650634	start codon destroyed	probably null	0.93
R5668:Vmn2r22	UTSW	6	123637914	missense	probably benign	0.06
R5776:Vmn2r22	UTSW	6	123637714	missense	probably damaging	1.00
R6416:Vmn2r22	UTSW	6	123637738	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AATCCCAGAATTTGATCCATGC -3'
(R):5'- CAGGGAATTATTCAACTGCTGC -3'

Sequencing Primer
(F):5'- AGAAAATGATAATCCTCCACCAAAG -3'
(R):5'- AACTGCTGCTATACTTCACTTGGG -3'

Posted On

2015-07-07