Mutagenetix > Incidental Mutation

Incidental Mutation 'R4347:Chil4'

328361

Institutional Source

Beutler Lab

Gene Symbol

Chil4

Ensembl Gene

ENSMUSG00000063779

Gene Name

chitinase-like 4

Synonyms

Chi3l4, Ym2

MMRRC Submission

041102-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4347 (G1)

Quality Score

203

Status

Validated

Chromosome

Chromosomal Location

106108807-106126795 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 106110144 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 317 (I317V)

Ref Sequence

ENSEMBL: ENSMUSP00000080851 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000082219]

AlphaFold

Q91Z98

Predicted Effect

probably benign
Transcript: ENSMUST00000082219
AA Change: I317V

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000080851
Gene: ENSMUSG00000063779
AA Change: I317V

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Glyco_18	22	365	1.77e-132	SMART

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 94.9%

Validation Efficiency

100% (32/32)

Allele List at MGI

Other mutations in this stock

Total: 25 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921536K21Rik	C	T	11: 3,840,122 (GRCm39)	V92M	probably damaging	Het
4930503E14Rik	C	A	14: 44,408,635 (GRCm39)	R66S	probably damaging	Het
Abca5	A	T	11: 110,190,794 (GRCm39)	I772N	probably damaging	Het
Acox1	T	C	11: 116,089,487 (GRCm39)	N15S	probably benign	Het
Arhgap28	C	T	17: 68,180,137 (GRCm39)	V233I	probably benign	Het
Dvl3	G	A	16: 20,350,049 (GRCm39)	R645H	possibly damaging	Het
Efl1	A	T	7: 82,347,174 (GRCm39)	M567L	probably damaging	Het
Gal3st2b	C	T	1: 93,867,530 (GRCm39)	T59I	probably damaging	Het
Gm7135	T	A	1: 97,276,035 (GRCm39)		noncoding transcript	Het
Gpihbp1	T	G	15: 75,470,017 (GRCm39)	*124G	probably null	Het
Igkv10-96	T	A	6: 68,609,164 (GRCm39)	R44W	probably benign	Het
Igkv13-84	T	A	6: 68,916,760 (GRCm39)	I19K	probably benign	Het
Kcnab1	A	G	3: 65,204,896 (GRCm39)		probably benign	Het
Kif1b	C	A	4: 149,331,691 (GRCm39)	G545C	probably damaging	Het
Mprip	T	C	11: 59,650,279 (GRCm39)	S1328P	possibly damaging	Het
Nrp1	T	C	8: 129,207,472 (GRCm39)		probably null	Het
Olfml1	C	T	7: 107,167,040 (GRCm39)	P23L	probably benign	Het
Plekhh2	G	A	17: 84,927,130 (GRCm39)	A1457T	probably benign	Het
Prr27	A	G	5: 87,990,531 (GRCm39)	I40V	possibly damaging	Het
Slc25a54	A	G	3: 109,010,055 (GRCm39)	T185A	possibly damaging	Het
Srek1	C	T	13: 103,885,267 (GRCm39)	G396D	probably null	Het
Syvn1	C	T	19: 6,099,951 (GRCm39)		probably benign	Het
Trim3	C	T	7: 105,268,594 (GRCm39)	G120D	probably damaging	Het
Usp5	T	A	6: 124,798,158 (GRCm39)	Q409L	probably damaging	Het
Vim	T	G	2: 13,580,329 (GRCm39)		probably benign	Het

Other mutations in Chil4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00778:Chil4	APN	3	106,109,113 (GRCm39)	missense	probably benign
IGL02457:Chil4	APN	3	106,121,715 (GRCm39)	missense	probably benign
R1087:Chil4	UTSW	3	106,117,881 (GRCm39)	missense	probably benign	0.01
R1398:Chil4	UTSW	3	106,126,825 (GRCm39)	splice site	probably null
R1503:Chil4	UTSW	3	106,113,350 (GRCm39)	missense	probably benign
R1553:Chil4	UTSW	3	106,111,006 (GRCm39)	missense	probably benign	0.02
R1806:Chil4	UTSW	3	106,117,959 (GRCm39)	splice site	probably benign
R1873:Chil4	UTSW	3	106,113,414 (GRCm39)	missense	probably benign	0.00
R2069:Chil4	UTSW	3	106,126,771 (GRCm39)	missense	probably benign	0.16
R2100:Chil4	UTSW	3	106,121,663 (GRCm39)	missense	probably benign
R2370:Chil4	UTSW	3	106,121,616 (GRCm39)	nonsense	probably null
R2984:Chil4	UTSW	3	106,111,043 (GRCm39)	missense	possibly damaging	0.95
R2985:Chil4	UTSW	3	106,111,043 (GRCm39)	missense	possibly damaging	0.95
R3522:Chil4	UTSW	3	106,111,056 (GRCm39)	missense	probably benign	0.08
R3919:Chil4	UTSW	3	106,109,848 (GRCm39)	missense	probably benign	0.00
R4033:Chil4	UTSW	3	106,121,765 (GRCm39)	missense	probably damaging	1.00
R4181:Chil4	UTSW	3	106,111,043 (GRCm39)	missense	possibly damaging	0.95
R4184:Chil4	UTSW	3	106,111,043 (GRCm39)	missense	possibly damaging	0.95
R4301:Chil4	UTSW	3	106,111,043 (GRCm39)	missense	possibly damaging	0.95
R4391:Chil4	UTSW	3	106,111,043 (GRCm39)	missense	possibly damaging	0.95
R4395:Chil4	UTSW	3	106,111,043 (GRCm39)	missense	possibly damaging	0.95
R4418:Chil4	UTSW	3	106,111,043 (GRCm39)	missense	possibly damaging	0.95
R4483:Chil4	UTSW	3	106,121,678 (GRCm39)	missense	probably damaging	1.00
R4544:Chil4	UTSW	3	106,117,922 (GRCm39)	missense	probably damaging	0.97
R4887:Chil4	UTSW	3	106,111,460 (GRCm39)	missense	probably benign	0.01
R4949:Chil4	UTSW	3	106,113,408 (GRCm39)	missense	possibly damaging	0.83
R5076:Chil4	UTSW	3	106,109,913 (GRCm39)	missense	probably damaging	1.00
R5146:Chil4	UTSW	3	106,110,150 (GRCm39)	missense	probably benign	0.18
R5254:Chil4	UTSW	3	106,126,768 (GRCm39)	missense	probably benign	0.00
R5521:Chil4	UTSW	3	106,111,013 (GRCm39)	missense	possibly damaging	0.50
R5790:Chil4	UTSW	3	106,109,894 (GRCm39)	missense	probably benign	0.00
R5883:Chil4	UTSW	3	106,117,886 (GRCm39)	missense	possibly damaging	0.48
R6010:Chil4	UTSW	3	106,121,711 (GRCm39)	missense	probably damaging	1.00
R6257:Chil4	UTSW	3	106,111,412 (GRCm39)	missense	possibly damaging	0.84
R6269:Chil4	UTSW	3	106,111,487 (GRCm39)	missense	probably damaging	1.00
R6602:Chil4	UTSW	3	106,117,906 (GRCm39)	missense	probably benign	0.00
R7113:Chil4	UTSW	3	106,121,664 (GRCm39)	missense	probably benign
R7113:Chil4	UTSW	3	106,110,083 (GRCm39)	missense	probably damaging	1.00
R7188:Chil4	UTSW	3	106,111,475 (GRCm39)	missense	probably damaging	1.00
R7980:Chil4	UTSW	3	106,110,060 (GRCm39)	missense	probably damaging	1.00
R8810:Chil4	UTSW	3	106,109,121 (GRCm39)	missense	probably damaging	0.99
R9300:Chil4	UTSW	3	106,109,874 (GRCm39)	missense	probably benign	0.10
R9307:Chil4	UTSW	3	106,111,382 (GRCm39)	critical splice donor site	probably null
R9529:Chil4	UTSW	3	106,118,656 (GRCm39)	missense	probably damaging	1.00
X0067:Chil4	UTSW	3	106,113,350 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TTGTTGTCCTTGAGCCACTGAG -3'
(R):5'- TAGTTGTGCCCTGTAGAACCAG -3'

Sequencing Primer
(F):5'- GAGCCACTGAGCCTGTTGAAAC -3'
(R):5'- CTGTAGAACCAGGATTCAGGG -3'

Posted On

2015-07-07