Mutagenetix > Incidental Mutation

Incidental Mutation 'R4430:Vmn2r22'

328562

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r22

Ensembl Gene

ENSMUSG00000095486

Gene Name

vomeronasal 2, receptor 22

Synonyms

EG546913

MMRRC Submission

041700-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R4430 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

123609758-123650635 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 123637858 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 258 (T258A)

Ref Sequence

ENSEMBL: ENSMUSP00000132043 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170808]

AlphaFold

E9Q7S8

Predicted Effect

possibly damaging
Transcript: ENSMUST00000170808
AA Change: T258A

PolyPhen 2 Score 0.468 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000132043
Gene: ENSMUSG00000095486
AA Change: T258A

Domain	Start	End	E-Value	Type
signal peptide	1	17	N/A	INTRINSIC
Pfam:ANF_receptor	81	473	1.4e-32	PFAM
Pfam:NCD3G	517	570	2.2e-23	PFAM
Pfam:7tm_3	601	838	1.2e-54	PFAM

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.1%
20x: 94.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610040J01Rik	G	T	5: 63,898,839		probably benign	Het
Aak1	A	G	6: 86,986,366	N926S	unknown	Het
Ahi1	T	A	10: 20,972,078	C462S	probably damaging	Het
Ahnak	A	G	19: 9,003,040	I563V	probably benign	Het
Ankrd55	G	A	13: 112,323,183		probably null	Het
Bag3	A	G	7: 128,523,923	D22G	probably damaging	Het
Cldn8	A	C	16: 88,562,731	M102R	probably damaging	Het
Col15a1	T	C	4: 47,245,705	F152S	probably damaging	Het
Cxcr2	T	C	1: 74,158,845	I166T	probably benign	Het
Dnah11	T	C	12: 117,983,011	I3113V	probably benign	Het
Gli2	T	C	1: 118,837,244	H1059R	probably benign	Het
Gm14412	C	A	2: 177,315,832	S90I	probably benign	Het
L1td1	A	G	4: 98,737,151	R528G	probably benign	Het
Mcm3	A	T	1: 20,811,993	L449*	probably null	Het
Mif4gd	T	C	11: 115,608,502	T185A	probably benign	Het
Mphosph9	T	C	5: 124,265,446	S840G	possibly damaging	Het
Nim1k	C	A	13: 119,712,542	R272L	possibly damaging	Het
Olfr1079	A	G	2: 86,538,387	I176T	probably damaging	Het
Olfr1457	C	T	19: 13,095,088	V187I	probably benign	Het
Olfr806	T	A	10: 129,738,261	I219F	probably damaging	Het
Pax3	A	G	1: 78,195,324	V83A	probably damaging	Het
Pde3b	C	T	7: 114,534,670	P974S	probably damaging	Het
Pdzd3	C	T	9: 44,249,744	S175N	probably benign	Het
Pglyrp3	T	A	3: 92,031,491	D324E	probably damaging	Het
Pus7	A	G	5: 23,746,489	Y521H	probably benign	Het
Ryr2	A	G	13: 11,735,527	S1953P	probably damaging	Het
Sost	G	A	11: 101,966,844	P44S	probably damaging	Het
Sox5	A	G	6: 144,041,274	I188T	possibly damaging	Het
Spata4	T	C	8: 54,601,843	I86T	probably benign	Het
Ssc5d	T	C	7: 4,943,664	S1006P	probably benign	Het
Stk10	T	C	11: 32,533,552	V50A	possibly damaging	Het
Sytl4	A	G,T	X: 133,949,223	S338R	probably damaging	Homo
Sytl5	A	T	X: 9,960,023	N412Y	probably damaging	Het
Tert	T	A	13: 73,627,475	F115Y	probably damaging	Het
Tmem181a	T	A	17: 6,295,786	L185H	probably damaging	Het
Tmem201	A	C	4: 149,731,139	V118G	probably benign	Het
Tmem67	T	A	4: 12,051,473	N785I	possibly damaging	Het
Trhde	A	T	10: 114,503,123	L594Q	probably damaging	Het
Ugt2b37	T	C	5: 87,254,092	M227V	probably benign	Het
Vmn2r73	A	T	7: 85,870,241	M503K	probably benign	Het
Zfp54	T	G	17: 21,434,960	V572G	probably damaging	Het

Other mutations in Vmn2r22

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01024:Vmn2r22	APN	6	123638053	missense	probably damaging	1.00
IGL01764:Vmn2r22	APN	6	123650420	critical splice donor site	probably null
IGL02037:Vmn2r22	APN	6	123649067	missense	probably damaging	1.00
IGL02183:Vmn2r22	APN	6	123638004	missense	probably damaging	1.00
IGL02335:Vmn2r22	APN	6	123638092	missense	probably damaging	0.99
IGL02440:Vmn2r22	APN	6	123637405	missense	probably benign	0.00
IGL02663:Vmn2r22	APN	6	123649158	missense	probably benign	0.11
IGL03101:Vmn2r22	APN	6	123637336	missense	probably benign	0.09
R0266:Vmn2r22	UTSW	6	123637404	missense	probably damaging	0.99
R0348:Vmn2r22	UTSW	6	123637725	missense	probably damaging	1.00
R0780:Vmn2r22	UTSW	6	123637974	missense	probably damaging	1.00
R0849:Vmn2r22	UTSW	6	123637404	missense	probably damaging	0.99
R1074:Vmn2r22	UTSW	6	123649258	missense	probably benign	0.02
R1456:Vmn2r22	UTSW	6	123637665	missense	possibly damaging	0.86
R1719:Vmn2r22	UTSW	6	123637843	missense	possibly damaging	0.68
R1989:Vmn2r22	UTSW	6	123637541	missense	probably damaging	1.00
R2928:Vmn2r22	UTSW	6	123637443	missense	probably damaging	0.96
R2939:Vmn2r22	UTSW	6	123637635	missense	probably damaging	0.99
R3727:Vmn2r22	UTSW	6	123650625	missense	possibly damaging	0.70
R3782:Vmn2r22	UTSW	6	123650632	nonsense	probably null
R3873:Vmn2r22	UTSW	6	123637380	missense	possibly damaging	0.68
R4344:Vmn2r22	UTSW	6	123637797	missense	probably damaging	1.00
R4407:Vmn2r22	UTSW	6	123637954	missense	probably damaging	1.00
R4428:Vmn2r22	UTSW	6	123637858	missense	possibly damaging	0.47
R4431:Vmn2r22	UTSW	6	123637858	missense	possibly damaging	0.47
R4701:Vmn2r22	UTSW	6	123650469	missense	probably benign	0.00
R5274:Vmn2r22	UTSW	6	123650634	start codon destroyed	probably null	0.93
R5668:Vmn2r22	UTSW	6	123637914	missense	probably benign	0.06
R5776:Vmn2r22	UTSW	6	123637714	missense	probably damaging	1.00
R6416:Vmn2r22	UTSW	6	123637738	missense	probably damaging	1.00
R7788:Vmn2r22	UTSW	6	123637600	missense	not run
R8208:Vmn2r22	UTSW	6	123637485	missense	probably damaging	1.00
R8267:Vmn2r22	UTSW	6	123638041	missense	possibly damaging	0.81
R8400:Vmn2r22	UTSW	6	123637527	nonsense	probably null
R8814:Vmn2r22	UTSW	6	123637830	missense	probably damaging	0.96
R8850:Vmn2r22	UTSW	6	123637495	missense	probably damaging	1.00
R9613:Vmn2r22	UTSW	6	123638116	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TCCCAGAATTTGATCCATGCGATC -3'
(R):5'- ATCAGGGAATTATTCAACTGCTGC -3'

Sequencing Primer
(F):5'- AGAAAATGATAATCCTCCACCAAAG -3'
(R):5'- AACTGCTGCTATACTTCACTTGGG -3'

Posted On

2015-07-21