Incidental Mutation 'R4447:Vmn1r228'
ID328813
Institutional Source Beutler Lab
Gene Symbol Vmn1r228
Ensembl Gene ENSMUSG00000060245
Gene Namevomeronasal 1 receptor 228
SynonymsV1re3
MMRRC Submission 041708-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.068) question?
Stock #R4447 (G1)
Quality Score225
Status Validated
Chromosome17
Chromosomal Location20776059-20777501 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to C at 20777107 bp
ZygosityHeterozygous
Amino Acid Change Isoleucine to Valine at position 50 (I50V)
Ref Sequence ENSEMBL: ENSMUSP00000072243 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000072410]
Predicted Effect probably damaging
Transcript: ENSMUST00000072410
AA Change: I50V

PolyPhen 2 Score 0.960 (Sensitivity: 0.78; Specificity: 0.95)
SMART Domains Protein: ENSMUSP00000072243
Gene: ENSMUSG00000060245
AA Change: I50V

DomainStartEndE-ValueType
Pfam:TAS2R 32 317 2.6e-11 PFAM
Pfam:7tm_1 53 316 2.6e-9 PFAM
Pfam:V1R 63 321 1.3e-26 PFAM
Meta Mutation Damage Score 0.6467 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.3%
  • 20x: 95.3%
Validation Efficiency 100% (32/32)
Allele List at MGI
Other mutations in this stock
Total: 30 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700014D04Rik T C 13: 59,742,198 T603A probably benign Het
3930402G23Rik T A 8: 10,926,129 noncoding transcript Het
Acsf2 G T 11: 94,569,359 P389Q possibly damaging Het
Aldh6a1 A G 12: 84,439,709 V120A possibly damaging Het
Alg11 C A 8: 22,068,079 A469E probably benign Het
Ankar A G 1: 72,687,789 S415P possibly damaging Het
Ano3 A T 2: 110,761,578 probably null Het
Asic4 A T 1: 75,470,370 probably benign Het
Atp1a4 A C 1: 172,234,431 I709S probably damaging Het
Cyp11b2 T C 15: 74,855,563 I90V probably benign Het
Fam129a A T 1: 151,636,402 probably null Het
Galnt2 A G 8: 124,295,377 D14G probably benign Het
Iqcm T C 8: 75,629,766 S176P probably damaging Het
Irf5 T A 6: 29,535,942 D318E probably damaging Het
Map2k3 A T 11: 60,947,171 S253C probably damaging Het
Mgst1 G T 6: 138,141,664 probably benign Het
Mipol1 G T 12: 57,352,748 probably benign Het
Olfr371 T A 8: 85,231,366 Y290* probably null Het
Pomgnt1 G T 4: 116,152,923 V161L possibly damaging Het
Rnpc3 T C 3: 113,611,137 probably benign Het
Rxfp1 C T 3: 79,652,127 probably benign Het
Scn5a T C 9: 119,550,627 D197G probably damaging Het
Thsd7a G A 6: 12,324,635 T1479I probably damaging Het
Twsg1 C T 17: 65,929,787 D83N possibly damaging Het
Ubqln3 C T 7: 104,142,814 R23Q probably benign Het
Wnk4 T A 11: 101,268,451 S565T possibly damaging Het
Wwc2 A G 8: 47,868,667 Y471H unknown Het
Zfp407 A T 18: 84,562,694 V98D possibly damaging Het
Zfp598 T A 17: 24,676,555 V73E probably damaging Het
Zscan29 T A 2: 121,169,886 probably null Het
Other mutations in Vmn1r228
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01368:Vmn1r228 APN 17 20776512 missense probably benign 0.07
IGL03275:Vmn1r228 APN 17 20776842 missense probably damaging 1.00
PIT4498001:Vmn1r228 UTSW 17 20776510 missense probably benign 0.00
R0097:Vmn1r228 UTSW 17 20776363 missense probably benign 0.05
R0097:Vmn1r228 UTSW 17 20776363 missense probably benign 0.05
R0270:Vmn1r228 UTSW 17 20776596 missense possibly damaging 0.60
R0279:Vmn1r228 UTSW 17 20776375 missense probably benign 0.02
R1544:Vmn1r228 UTSW 17 20777023 missense probably benign 0.00
R1695:Vmn1r228 UTSW 17 20776298 missense possibly damaging 0.49
R2086:Vmn1r228 UTSW 17 20777193 missense possibly damaging 0.71
R2275:Vmn1r228 UTSW 17 20776545 missense probably damaging 1.00
R2965:Vmn1r228 UTSW 17 20776347 missense probably damaging 0.99
R4425:Vmn1r228 UTSW 17 20776599 missense probably damaging 1.00
R5031:Vmn1r228 UTSW 17 20776681 nonsense probably null
R6345:Vmn1r228 UTSW 17 20776882 missense probably damaging 1.00
R7064:Vmn1r228 UTSW 17 20777023 missense probably benign 0.00
R7880:Vmn1r228 UTSW 17 20776410 missense probably damaging 1.00
R8000:Vmn1r228 UTSW 17 20776965 missense possibly damaging 0.88
R8290:Vmn1r228 UTSW 17 20776462 missense probably benign 0.09
X0018:Vmn1r228 UTSW 17 20776701 missense probably benign 0.08
Predicted Primers PCR Primer
(F):5'- AGCTTGCACCCAACATTATTG -3'
(R):5'- CAGTCTTGCTATGATGTCCACC -3'

Sequencing Primer
(F):5'- TGAATAACTGCTTTGACCCAAAAGC -3'
(R):5'- GTCCACCATGACTTTGAACATAGGTG -3'
Posted On2015-07-21