Mutagenetix > Incidental Mutation

Incidental Mutation 'R4455:Mslnl'

329168

Institutional Source

Beutler Lab

Gene Symbol

Mslnl

Ensembl Gene

ENSMUSG00000041062

Gene Name

mesothelin-like

Synonyms

MMRRC Submission

041715-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4455 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

25736040-25748330 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 25742934 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Methionine at position 128 (V128M)

Ref Sequence

ENSEMBL: ENSMUSP00000049020 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047098]

AlphaFold

Q8C160

Predicted Effect

probably damaging
Transcript: ENSMUST00000047098
AA Change: V128M

PolyPhen 2 Score 0.971 (Sensitivity: 0.77; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000049020
Gene: ENSMUSG00000041062
AA Change: V128M

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:Mesothelin	29	589	2.8e-70	PFAM
low complexity region	633	653	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000102319

Meta Mutation Damage Score

0.2148

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.1%
20x: 95.0%

Validation Efficiency

100% (61/61)

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700102P08Rik	A	G	9: 108,397,196	H166R	possibly damaging	Het
Aktip	T	C	8: 91,124,851	E248G	probably benign	Het
Ankk1	A	G	9: 49,418,066	V336A	probably benign	Het
Aoc1	T	A	6: 48,905,467	D92E	probably damaging	Het
Arfgef2	T	C	2: 166,894,715	I1769T	probably benign	Het
Arfgef3	A	T	10: 18,607,675	S1434T	probably benign	Het
Baz1a	C	A	12: 54,911,368	V1033L	probably benign	Het
Bbs12	T	C	3: 37,320,312	V418A	probably damaging	Het
Cacnb4	A	G	2: 52,465,653	V214A	probably damaging	Het
Camk2d	T	C	3: 126,780,403	V153A	probably damaging	Het
Ccdc18	C	T	5: 108,161,529	S330L	possibly damaging	Het
Cdh11	T	C	8: 102,647,823	D500G	probably benign	Het
Cdkn2d	C	G	9: 21,290,889	V21L	probably benign	Het
Clca4a	G	A	3: 144,957,259	P610S	probably damaging	Het
Dctn1	T	C	6: 83,195,049	L807P	probably damaging	Het
Dopey2	T	C	16: 93,766,215	L869P	probably damaging	Het
Egr2	GAA	GA	10: 67,539,903		probably null	Het
Eya1	C	T	1: 14,183,196	V519M	probably damaging	Het
Fam227b	T	A	2: 126,146,268		probably benign	Het
Fam26f	A	T	10: 34,126,535	I184N	probably damaging	Het
Fsip2	G	T	2: 82,990,776	A5618S	possibly damaging	Het
Grb10	T	G	11: 11,967,665	Q72P	possibly damaging	Het
H3f3a	G	T	1: 180,803,103	R129S	probably benign	Het
Hfm1	T	C	5: 106,886,508		probably null	Het
Kansl1	T	C	11: 104,424,358	T285A	possibly damaging	Het
Krtap16-1	A	T	11: 99,985,733	C282S	probably benign	Het
Magi1	A	G	6: 93,785,457	V89A	probably damaging	Het
Mllt1	A	G	17: 56,919,965	Y71H	probably damaging	Het
Ms4a14	T	A	19: 11,303,626	T523S	possibly damaging	Het
Muc5b	T	C	7: 141,858,818	S1834P	unknown	Het
Necap1	C	T	6: 122,887,369	S270F	possibly damaging	Het
Piwil2	T	C	14: 70,390,565	M752V	probably benign	Het
Prune1	G	A	3: 95,281,896		probably null	Het
Ptpro	A	G	6: 137,393,659	E586G	probably damaging	Het
Rela	T	A	19: 5,647,262	I499K	probably damaging	Het
Rpl31-ps17	C	T	12: 54,701,612		noncoding transcript	Het
Scara5	T	A	14: 65,762,747	D455E	probably benign	Het
Slc2a4	G	A	11: 69,943,322		probably benign	Het
Sntb2	G	A	8: 106,991,607		probably null	Het
Sspo	C	T	6: 48,465,516	R1982C	probably damaging	Het
Svs1	A	T	6: 48,987,460	N134I	possibly damaging	Het
Tsen34	G	A	7: 3,695,098		probably null	Het
Ttc28	AC	A	5: 111,224,058		probably null	Het
Ttn	T	C	2: 76,946,913	M1382V	probably benign	Het
Utp18	G	A	11: 93,885,447	R71C	probably benign	Het
Xrn1	T	A	9: 95,973,645		probably benign	Het
Yeats2	A	G	16: 20,161,993	K187R	possibly damaging	Het

Other mutations in Mslnl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01621:Mslnl	APN	17	25743667	unclassified	probably benign
IGL01629:Mslnl	APN	17	25744775	missense	possibly damaging	0.95
IGL02084:Mslnl	APN	17	25746151	missense	probably benign	0.07
IGL02408:Mslnl	APN	17	25747998	missense	possibly damaging	0.80
IGL02726:Mslnl	APN	17	25744103	critical splice donor site	probably null
IGL03387:Mslnl	APN	17	25744077	missense	probably benign	0.06
R0561:Mslnl	UTSW	17	25743203	nonsense	probably null
R0881:Mslnl	UTSW	17	25742965	missense	possibly damaging	0.82
R1295:Mslnl	UTSW	17	25743240	missense	probably damaging	1.00
R1296:Mslnl	UTSW	17	25743240	missense	probably damaging	1.00
R1582:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1629:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1630:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1631:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1632:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1794:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1850:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1866:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1876:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1914:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2166:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2241:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2243:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2247:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2282:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2284:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2852:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2877:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2878:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2919:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2920:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3026:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3405:Mslnl	UTSW	17	25746181	missense	probably damaging	1.00
R3406:Mslnl	UTSW	17	25746181	missense	probably damaging	1.00
R3411:Mslnl	UTSW	17	25744517	missense	probably benign	0.05
R3434:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3546:Mslnl	UTSW	17	25744969	missense	probably damaging	0.98
R3612:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3729:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3730:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3802:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3804:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3894:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3895:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4454:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4456:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4457:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4561:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4562:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4564:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4600:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4601:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4610:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4704:Mslnl	UTSW	17	25738978	missense	possibly damaging	0.73
R5155:Mslnl	UTSW	17	25738968	nonsense	probably null
R5257:Mslnl	UTSW	17	25746165	missense	probably benign	0.00
R5456:Mslnl	UTSW	17	25743159	missense	probably damaging	0.98
R5645:Mslnl	UTSW	17	25737842	missense	possibly damaging	0.95
R6007:Mslnl	UTSW	17	25746775	missense	probably benign	0.00
R6083:Mslnl	UTSW	17	25737902	missense	possibly damaging	0.83
R6142:Mslnl	UTSW	17	25744557	missense	probably damaging	1.00
R6761:Mslnl	UTSW	17	25746073	missense	probably damaging	1.00
R7058:Mslnl	UTSW	17	25743212	missense	probably benign	0.03
R7156:Mslnl	UTSW	17	25743210	missense	probably benign	0.20
R7467:Mslnl	UTSW	17	25736921	start codon destroyed	probably benign	0.33
R7687:Mslnl	UTSW	17	25743183	missense	probably damaging	0.97
R7807:Mslnl	UTSW	17	25746777	missense	probably benign	0.03
R8682:Mslnl	UTSW	17	25746988	missense	probably benign
R8735:Mslnl	UTSW	17	25745088	missense	probably benign	0.09
R8742:Mslnl	UTSW	17	25745073	missense	probably damaging	1.00
R9208:Mslnl	UTSW	17	25742720	missense	possibly damaging	0.94
R9264:Mslnl	UTSW	17	25742532	intron	probably benign
RF007:Mslnl	UTSW	17	25743228	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- CCATCAGAAGGGAGGTTTGAAC -3'
(R):5'- ACAGGTCTGAAGCACTGAGC -3'

Sequencing Primer
(F):5'- TTTGAACCCAGGACTGAGGTGAC -3'
(R):5'- GCCCTGCCGAGACAGTAG -3'

Posted On

2015-07-21