Mutagenetix > Incidental Mutation

Incidental Mutation 'R4470:Vmn1r68'

329366

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r68

Ensembl Gene

ENSMUSG00000047031

Gene Name

vomeronasal 1 receptor 68

Synonyms

Gm6898

MMRRC Submission

041727-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.055) question?

Stock #

R4470 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

10261137-10262096 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 10261275 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Lysine at position 274 (N274K)

Ref Sequence

ENSEMBL: ENSMUSP00000050927 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000058362]

AlphaFold

E9Q0V3

Predicted Effect

probably benign
Transcript: ENSMUST00000058362
AA Change: N274K

PolyPhen 2 Score 0.227 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000050927
Gene: ENSMUSG00000047031
AA Change: N274K

Domain	Start	End	E-Value	Type
Pfam:V1R	49	306	2e-33	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.0%
20x: 94.8%

Validation Efficiency

100% (56/56)

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts1	A	C	16: 85,595,404 (GRCm39)	V258G	possibly damaging	Het
Adcy1	A	G	11: 7,094,804 (GRCm39)	T577A	probably benign	Het
Adgb	T	C	10: 10,274,695 (GRCm39)	N728S	probably benign	Het
Alpk1	A	G	3: 127,473,175 (GRCm39)	S943P	probably damaging	Het
Ankdd1a	T	C	9: 65,410,791 (GRCm39)	D398G	probably damaging	Het
Asah1	A	G	8: 41,796,761 (GRCm39)		probably null	Het
Atp13a1	T	C	8: 70,251,329 (GRCm39)	S495P	probably damaging	Het
Camta2	T	C	11: 70,571,766 (GRCm39)	D510G	probably damaging	Het
Cdh8	T	A	8: 100,143,321 (GRCm39)		probably benign	Het
Cul9	C	T	17: 46,849,262 (GRCm39)	A628T	probably benign	Het
Ddx19a	A	G	8: 111,703,111 (GRCm39)	V442A	probably benign	Het
Ddx41	G	A	13: 55,682,293 (GRCm39)	R205W	possibly damaging	Het
Dnah7c	T	A	1: 46,787,795 (GRCm39)	S3283T	possibly damaging	Het
Fmo9	T	A	1: 166,507,799 (GRCm39)	I19F	probably damaging	Het
Gmeb2	A	T	2: 180,906,938 (GRCm39)		probably null	Het
H2-K2	G	A	17: 34,219,035 (GRCm39)	R23C	probably benign	Het
Icam5	T	A	9: 20,946,802 (GRCm39)	C443*	probably null	Het
Il16	A	G	7: 83,300,046 (GRCm39)		probably benign	Het
Kcnj3	A	T	2: 55,327,877 (GRCm39)	H222L	probably damaging	Het
Lama4	T	A	10: 38,956,492 (GRCm39)	Y1138*	probably null	Het
Lpin3	A	G	2: 160,737,354 (GRCm39)	T134A	probably benign	Het
Man1b1	C	G	2: 25,222,867 (GRCm39)		probably benign	Het
Msc	G	C	1: 14,825,902 (GRCm39)	P24R	probably damaging	Het
Mylk	A	G	16: 34,732,522 (GRCm39)	K601E	probably benign	Het
Myzap	A	G	9: 71,499,563 (GRCm39)		probably benign	Het
Ncor2	A	T	5: 125,179,705 (GRCm39)		probably null	Het
Nrxn3	T	C	12: 90,171,515 (GRCm39)	S276P	probably damaging	Het
Or1j16	T	C	2: 36,530,233 (GRCm39)	F61L	probably damaging	Het
Or4c114	T	C	2: 88,905,382 (GRCm39)	N18D	probably benign	Het
Or4d6	T	C	19: 12,086,547 (GRCm39)		probably null	Het
Or51b4	G	A	7: 103,530,956 (GRCm39)	P165S	probably benign	Het
Or6c202	G	A	10: 128,996,383 (GRCm39)	L157F	probably benign	Het
Or8c13	A	G	9: 38,091,666 (GRCm39)	M151T	probably damaging	Het
Or8d1	T	C	9: 38,766,927 (GRCm39)	S190P	probably damaging	Het
Pramel5	T	C	4: 143,997,915 (GRCm39)	K443E	possibly damaging	Het
Rock2	C	T	12: 17,021,276 (GRCm39)	Q1019*	probably null	Het
Rps18-ps3	T	A	8: 107,989,762 (GRCm39)		noncoding transcript	Het
Sis	T	C	3: 72,835,492 (GRCm39)		probably null	Het
Slc22a21	T	A	11: 53,846,839 (GRCm39)	I345F	probably benign	Het
Sox5	A	G	6: 143,790,491 (GRCm39)	M523T	possibly damaging	Het
Taf1c	T	C	8: 120,326,361 (GRCm39)	K526R	probably benign	Het
Taf2	T	C	15: 54,922,276 (GRCm39)	D337G	possibly damaging	Het
Tbx4	A	G	11: 85,802,948 (GRCm39)	I275V	probably damaging	Het
Tmem59l	A	G	8: 70,939,951 (GRCm39)	L6S	unknown	Het
Tmem9	A	G	1: 135,955,234 (GRCm39)	T123A	probably benign	Het
Trim69	A	G	2: 122,009,080 (GRCm39)	Y380C	probably damaging	Het
Vldlr	G	T	19: 27,212,219 (GRCm39)	V78L	probably damaging	Het
Vmn2r104	G	T	17: 20,262,503 (GRCm39)	S209Y	probably damaging	Het
Vmn2r108	T	A	17: 20,682,990 (GRCm39)	N738I	probably damaging	Het
Wnt3	A	G	11: 103,703,450 (GRCm39)	D311G	probably damaging	Het
Yme1l1	T	C	2: 23,076,344 (GRCm39)		probably null	Het
Zcchc14	C	T	8: 122,378,498 (GRCm39)		probably benign	Het
Zfp148	T	C	16: 33,316,602 (GRCm39)	Y383H	probably damaging	Het
Zfp41	A	G	15: 75,490,368 (GRCm39)	R107G	probably damaging	Het

Other mutations in Vmn1r68

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01892:Vmn1r68	APN	7	10,261,334 (GRCm39)	missense	possibly damaging	0.76
IGL02051:Vmn1r68	APN	7	10,261,948 (GRCm39)	missense	probably benign	0.00
IGL02112:Vmn1r68	APN	7	10,261,787 (GRCm39)	missense	probably damaging	0.96
IGL02619:Vmn1r68	APN	7	10,261,603 (GRCm39)	missense	probably benign	0.14
IGL03033:Vmn1r68	APN	7	10,262,074 (GRCm39)	missense	probably damaging	0.99
IGL03184:Vmn1r68	APN	7	10,261,799 (GRCm39)	missense	probably benign	0.16
PIT4354001:Vmn1r68	UTSW	7	10,261,958 (GRCm39)	missense	probably benign
R0141:Vmn1r68	UTSW	7	10,261,252 (GRCm39)	missense	possibly damaging	0.69
R0359:Vmn1r68	UTSW	7	10,261,201 (GRCm39)	missense	probably damaging	1.00
R0634:Vmn1r68	UTSW	7	10,261,162 (GRCm39)	missense	probably benign	0.00
R1731:Vmn1r68	UTSW	7	10,261,802 (GRCm39)	missense	probably damaging	1.00
R2021:Vmn1r68	UTSW	7	10,261,918 (GRCm39)	missense	probably damaging	1.00
R2022:Vmn1r68	UTSW	7	10,261,918 (GRCm39)	missense	probably damaging	1.00
R2243:Vmn1r68	UTSW	7	10,262,089 (GRCm39)	missense	probably damaging	0.98
R2262:Vmn1r68	UTSW	7	10,261,372 (GRCm39)	missense	probably damaging	1.00
R3877:Vmn1r68	UTSW	7	10,261,408 (GRCm39)	missense	probably damaging	1.00
R4843:Vmn1r68	UTSW	7	10,261,904 (GRCm39)	missense	probably benign	0.00
R5198:Vmn1r68	UTSW	7	10,261,723 (GRCm39)	missense	probably benign	0.00
R5835:Vmn1r68	UTSW	7	10,261,747 (GRCm39)	missense	possibly damaging	0.68
R5906:Vmn1r68	UTSW	7	10,261,550 (GRCm39)	missense	probably benign	0.02
R6015:Vmn1r68	UTSW	7	10,261,616 (GRCm39)	missense	probably benign	0.31
R7073:Vmn1r68	UTSW	7	10,261,778 (GRCm39)	missense	probably benign	0.00
R7614:Vmn1r68	UTSW	7	10,261,553 (GRCm39)	missense	probably benign	0.05
R7699:Vmn1r68	UTSW	7	10,261,559 (GRCm39)	missense	probably benign	0.15
R7700:Vmn1r68	UTSW	7	10,261,559 (GRCm39)	missense	probably benign	0.15
R7912:Vmn1r68	UTSW	7	10,261,237 (GRCm39)	missense	probably benign	0.01
R8166:Vmn1r68	UTSW	7	10,261,888 (GRCm39)	missense	probably benign	0.10
R8426:Vmn1r68	UTSW	7	10,261,382 (GRCm39)	missense	probably benign	0.02
R9466:Vmn1r68	UTSW	7	10,261,417 (GRCm39)	missense	probably damaging	1.00
R9466:Vmn1r68	UTSW	7	10,261,317 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- ATCCAGCAAGCTCCCATAATTG -3'
(R):5'- TGGCATCCTGTACAGACACAAG -3'

Sequencing Primer
(F):5'- AGCAAGCTCCCATAATTGTTTCTG -3'
(R):5'- GCAGAGGGTTCAACATATCCGC -3'

Posted On

2015-07-21