Incidental Mutation 'R4471:Tecrl'
ID 329421
Institutional Source Beutler Lab
Gene Symbol Tecrl
Ensembl Gene ENSMUSG00000049537
Gene Name trans-2,3-enoyl-CoA reductase-like
Synonyms Srd5a2l2, D330017N19Rik
MMRRC Submission 041728-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.064) question?
Stock # R4471 (G1)
Quality Score 225
Status Validated
Chromosome 5
Chromosomal Location 83425992-83503042 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 83461134 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Tyrosine to Histidine at position 108 (Y108H)
Ref Sequence ENSEMBL: ENSMUSP00000062122 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000053543]
AlphaFold Q8BFZ1
Predicted Effect probably benign
Transcript: ENSMUST00000053543
AA Change: Y108H

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000062122
Gene: ENSMUSG00000049537
AA Change: Y108H

DomainStartEndE-ValueType
PDB:2DZJ|A 53 135 1e-17 PDB
Blast:UBQ 59 135 2e-7 BLAST
transmembrane domain 139 161 N/A INTRINSIC
Pfam:Steroid_dh 208 361 3.8e-22 PFAM
Predicted Effect unknown
Transcript: ENSMUST00000146669
AA Change: Y93H
SMART Domains Protein: ENSMUSP00000115144
Gene: ENSMUSG00000049537
AA Change: Y93H

DomainStartEndE-ValueType
PDB:2DZJ|A 39 93 1e-11 PDB
transmembrane domain 100 122 N/A INTRINSIC
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.3%
  • 20x: 95.4%
Validation Efficiency 99% (69/70)
Allele List at MGI
Other mutations in this stock
Total: 65 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Alpk1 A G 3: 127,473,175 (GRCm39) S943P probably damaging Het
Ankdd1a T C 9: 65,410,791 (GRCm39) D398G probably damaging Het
Ankrd13b T A 11: 77,367,040 (GRCm39) K49M probably damaging Het
Asah1 A G 8: 41,796,761 (GRCm39) probably null Het
Cdc34b A G 11: 94,633,064 (GRCm39) E88G probably benign Het
Cdh2 T C 18: 16,907,533 (GRCm39) probably null Het
Ceacam1 T A 7: 25,174,025 (GRCm39) N210I possibly damaging Het
Celf3 A G 3: 94,395,585 (GRCm39) probably null Het
Cfap20dc A G 14: 8,536,571 (GRCm38) L212P probably damaging Het
Cmya5 A T 13: 93,228,833 (GRCm39) M2085K probably benign Het
Cpne6 G A 14: 55,754,089 (GRCm39) V469M probably damaging Het
Cyp2j12 A G 4: 96,021,306 (GRCm39) V100A probably benign Het
Dnajb4 A T 3: 151,890,799 (GRCm39) H333Q probably benign Het
Eif3f T C 7: 108,540,153 (GRCm39) V316A possibly damaging Het
Eml1 T C 12: 108,472,894 (GRCm39) probably benign Het
Ern1 C A 11: 106,310,868 (GRCm39) V302L possibly damaging Het
Fam110b A T 4: 5,799,092 (GRCm39) H170L probably benign Het
Fn3krp T G 11: 121,317,499 (GRCm39) D146E probably benign Het
Git1 A G 11: 77,390,650 (GRCm39) T129A probably benign Het
Glrp1 T A 1: 88,431,196 (GRCm39) Q58L unknown Het
Gpatch2 A G 1: 186,965,337 (GRCm39) E281G probably damaging Het
Hipk2 T A 6: 38,713,857 (GRCm39) probably benign Het
Hydin A G 8: 111,313,764 (GRCm39) N4214S probably damaging Het
Icam5 T A 9: 20,946,802 (GRCm39) C443* probably null Het
Impdh1 G A 6: 29,204,631 (GRCm39) Q307* probably null Het
Ivns1abp C T 1: 151,236,990 (GRCm39) T447M probably benign Het
Lman1 A T 18: 66,124,797 (GRCm39) probably benign Het
Mdn1 A G 4: 32,668,860 (GRCm39) E306G probably benign Het
Msc G C 1: 14,825,902 (GRCm39) P24R probably damaging Het
Mtif2 T C 11: 29,490,053 (GRCm39) probably benign Het
Mvp C T 7: 126,601,130 (GRCm39) M1I probably null Het
Myh7 A T 14: 55,229,311 (GRCm39) Y162* probably null Het
Nemf G A 12: 69,361,216 (GRCm39) H956Y probably benign Het
Nktr T C 9: 121,577,962 (GRCm39) probably benign Het
Nrxn3 T C 12: 90,171,515 (GRCm39) S276P probably damaging Het
Or8d1 T C 9: 38,766,927 (GRCm39) S190P probably damaging Het
Patj A G 4: 98,423,816 (GRCm39) K621E probably damaging Het
Prss2 A G 6: 41,499,780 (GRCm39) I24V probably damaging Het
R3hcc1l T C 19: 42,571,259 (GRCm39) probably benign Het
Rest T C 5: 77,429,027 (GRCm39) V482A probably benign Het
Rexo1 A T 10: 80,378,492 (GRCm39) S476T probably damaging Het
Rin2 C T 2: 145,702,366 (GRCm39) T354I probably benign Het
Slc16a3 T C 11: 120,846,774 (GRCm39) probably benign Het
Slc5a4b G A 10: 75,894,725 (GRCm39) Q594* probably null Het
Slk T G 19: 47,603,862 (GRCm39) V202G probably damaging Het
Smarca2 T A 19: 26,597,277 (GRCm39) V53D possibly damaging Het
Snrnp200 T A 2: 127,080,673 (GRCm39) V2036E probably benign Het
Sox5 A G 6: 143,790,491 (GRCm39) M523T possibly damaging Het
Syt17 A G 7: 118,036,040 (GRCm39) probably null Het
Taf2 T C 15: 54,922,276 (GRCm39) D337G possibly damaging Het
Tcstv2a G T 13: 120,725,579 (GRCm39) R81L probably benign Het
Tln1 A T 4: 43,551,018 (GRCm39) F409L probably benign Het
Tmem59l A G 8: 70,939,951 (GRCm39) L6S unknown Het
Ttc23 G A 7: 67,319,904 (GRCm39) R187Q probably benign Het
Ttl T C 2: 128,923,977 (GRCm39) V230A probably benign Het
Ube2u G A 4: 100,338,843 (GRCm39) W36* probably null Het
Ube4a A T 9: 44,857,830 (GRCm39) probably benign Het
Ulk1 C T 5: 110,937,223 (GRCm39) R691Q probably benign Het
Unc45a A G 7: 79,982,728 (GRCm39) I399T possibly damaging Het
Utp25 C T 1: 192,812,445 (GRCm39) R5Q possibly damaging Het
Wdr59 A C 8: 112,193,419 (GRCm39) probably null Het
Zfp24 A G 18: 24,151,172 (GRCm39) probably benign Het
Zfp777 A T 6: 48,019,041 (GRCm39) W342R probably damaging Het
Zfp979 A T 4: 147,697,913 (GRCm39) C265* probably null Het
Zzef1 T A 11: 72,804,157 (GRCm39) L2633Q probably damaging Het
Other mutations in Tecrl
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01981:Tecrl APN 5 83,442,453 (GRCm39) missense probably benign
IGL02067:Tecrl APN 5 83,432,122 (GRCm39) missense probably benign 0.05
IGL02111:Tecrl APN 5 83,502,639 (GRCm39) missense probably damaging 1.00
IGL02391:Tecrl APN 5 83,502,674 (GRCm39) missense probably benign 0.00
IGL02860:Tecrl APN 5 83,502,844 (GRCm39) missense probably benign 0.01
IGL03079:Tecrl APN 5 83,442,447 (GRCm39) missense probably damaging 0.96
IGL03109:Tecrl APN 5 83,457,156 (GRCm39) intron probably benign
gaudenz UTSW 5 83,457,049 (GRCm39) critical splice donor site probably null
Whoopie UTSW 5 83,442,453 (GRCm39) missense probably damaging 1.00
BB004:Tecrl UTSW 5 83,502,666 (GRCm39) missense probably damaging 1.00
BB014:Tecrl UTSW 5 83,502,666 (GRCm39) missense probably damaging 1.00
R0095:Tecrl UTSW 5 83,442,417 (GRCm39) splice site probably benign
R0347:Tecrl UTSW 5 83,442,479 (GRCm39) missense probably damaging 1.00
R0372:Tecrl UTSW 5 83,442,506 (GRCm39) missense probably damaging 1.00
R0403:Tecrl UTSW 5 83,502,605 (GRCm39) splice site probably benign
R0426:Tecrl UTSW 5 83,502,610 (GRCm39) splice site probably benign
R0597:Tecrl UTSW 5 83,502,775 (GRCm39) nonsense probably null
R1607:Tecrl UTSW 5 83,428,355 (GRCm39) splice site probably null
R1771:Tecrl UTSW 5 83,439,134 (GRCm39) missense probably damaging 1.00
R1800:Tecrl UTSW 5 83,427,077 (GRCm39) missense probably damaging 1.00
R1815:Tecrl UTSW 5 83,427,081 (GRCm39) missense probably benign 0.01
R1869:Tecrl UTSW 5 83,502,706 (GRCm39) missense probably benign 0.00
R1870:Tecrl UTSW 5 83,502,706 (GRCm39) missense probably benign 0.00
R4296:Tecrl UTSW 5 83,461,174 (GRCm39) nonsense probably null
R6281:Tecrl UTSW 5 83,442,453 (GRCm39) missense probably damaging 1.00
R6343:Tecrl UTSW 5 83,442,447 (GRCm39) missense probably damaging 0.96
R6866:Tecrl UTSW 5 83,461,161 (GRCm39) missense probably damaging 1.00
R6948:Tecrl UTSW 5 83,457,097 (GRCm39) missense probably benign
R6971:Tecrl UTSW 5 83,502,649 (GRCm39) missense possibly damaging 0.58
R6981:Tecrl UTSW 5 83,502,768 (GRCm39) missense possibly damaging 0.83
R7246:Tecrl UTSW 5 83,427,182 (GRCm39) missense probably damaging 0.99
R7282:Tecrl UTSW 5 83,502,754 (GRCm39) missense probably benign 0.26
R7444:Tecrl UTSW 5 83,502,915 (GRCm39) unclassified probably benign
R7900:Tecrl UTSW 5 83,427,188 (GRCm39) missense probably benign 0.04
R7927:Tecrl UTSW 5 83,502,666 (GRCm39) missense probably damaging 1.00
R8246:Tecrl UTSW 5 83,427,156 (GRCm39) missense probably damaging 0.99
R8360:Tecrl UTSW 5 83,448,764 (GRCm39) missense probably damaging 1.00
R8466:Tecrl UTSW 5 83,428,367 (GRCm39) nonsense probably null
R8947:Tecrl UTSW 5 83,461,154 (GRCm39) missense probably benign 0.10
R8949:Tecrl UTSW 5 83,461,154 (GRCm39) missense probably benign 0.10
R9009:Tecrl UTSW 5 83,432,121 (GRCm39) missense probably damaging 0.96
R9115:Tecrl UTSW 5 83,427,906 (GRCm39) missense possibly damaging 0.93
R9182:Tecrl UTSW 5 83,457,049 (GRCm39) critical splice donor site probably null
R9320:Tecrl UTSW 5 83,428,422 (GRCm39) missense possibly damaging 0.67
X0019:Tecrl UTSW 5 83,486,099 (GRCm39) missense probably benign 0.32
X0024:Tecrl UTSW 5 83,486,099 (GRCm39) missense probably benign 0.32
X0034:Tecrl UTSW 5 83,486,099 (GRCm39) missense probably benign 0.32
X0035:Tecrl UTSW 5 83,486,099 (GRCm39) missense probably benign 0.32
X0036:Tecrl UTSW 5 83,486,099 (GRCm39) missense probably benign 0.32
X0037:Tecrl UTSW 5 83,486,099 (GRCm39) missense probably benign 0.32
X0038:Tecrl UTSW 5 83,486,099 (GRCm39) missense probably benign 0.32
X0039:Tecrl UTSW 5 83,486,099 (GRCm39) missense probably benign 0.32
X0040:Tecrl UTSW 5 83,486,099 (GRCm39) missense probably benign 0.32
X0052:Tecrl UTSW 5 83,486,099 (GRCm39) missense probably benign 0.32
X0053:Tecrl UTSW 5 83,486,099 (GRCm39) missense probably benign 0.32
X0054:Tecrl UTSW 5 83,486,099 (GRCm39) missense probably benign 0.32
X0058:Tecrl UTSW 5 83,486,099 (GRCm39) missense probably benign 0.32
X0060:Tecrl UTSW 5 83,486,099 (GRCm39) missense probably benign 0.32
X0061:Tecrl UTSW 5 83,486,099 (GRCm39) missense probably benign 0.32
X0062:Tecrl UTSW 5 83,486,099 (GRCm39) missense probably benign 0.32
X0063:Tecrl UTSW 5 83,486,099 (GRCm39) missense probably benign 0.32
Predicted Primers PCR Primer
(F):5'- GCTAGAGAATGTTGCATTTTCCCC -3'
(R):5'- GCACTCTTCCAATGAGAATTACTG -3'

Sequencing Primer
(F):5'- GAATGTTGCATTTTCCCCAAATTTC -3'
(R):5'- TTCCAATGAGAATTACTGAGTATGTG -3'
Posted On 2015-07-21