Mutagenetix > Incidental Mutation

Incidental Mutation 'R4438:Zfp91'

329646

Institutional Source

Beutler Lab

Gene Symbol

Zfp91

Ensembl Gene

ENSMUSG00000024695

Gene Name

zinc finger protein 91

Synonyms

Pzf, 9130014I08Rik, A530054C17Rik

MMRRC Submission

041703-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.482) question?

Stock #

R4438 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

12744384-12773490 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to A at 12755385 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Stop codon at position 217 (E217*)

Ref Sequence

ENSEMBL: ENSMUSP00000124424 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000038627] [ENSMUST00000142247]

AlphaFold

Q62511

Predicted Effect

probably null
Transcript: ENSMUST00000038627
AA Change: E217*

SMART Domains

Protein: ENSMUSP00000037971
Gene: ENSMUSG00000024695
AA Change: E217*

Domain	Start	End	E-Value	Type
low complexity region	21	27	N/A	INTRINSIC
low complexity region	35	43	N/A	INTRINSIC
low complexity region	72	92	N/A	INTRINSIC
low complexity region	98	114	N/A	INTRINSIC
low complexity region	120	135	N/A	INTRINSIC
low complexity region	143	167	N/A	INTRINSIC
low complexity region	207	226	N/A	INTRINSIC
coiled coil region	256	284	N/A	INTRINSIC
ZnF_C2H2	313	338	1.08e-1	SMART
ZnF_C2H2	344	368	7.15e-2	SMART
ZnF_C2H2	374	396	1.56e-2	SMART
ZnF_C2H2	402	424	2.61e-4	SMART
ZnF_C2H2	432	455	1.92e-2	SMART
low complexity region	459	471	N/A	INTRINSIC
low complexity region	491	502	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000137256

Predicted Effect

probably null
Transcript: ENSMUST00000142247
AA Change: E217*

SMART Domains

Protein: ENSMUSP00000124424
Gene: ENSMUSG00000024695
AA Change: E217*

Domain	Start	End	E-Value	Type
low complexity region	21	27	N/A	INTRINSIC
low complexity region	35	43	N/A	INTRINSIC
low complexity region	72	92	N/A	INTRINSIC
low complexity region	98	114	N/A	INTRINSIC
low complexity region	120	135	N/A	INTRINSIC
low complexity region	143	167	N/A	INTRINSIC
low complexity region	207	226	N/A	INTRINSIC
coiled coil region	256	284	N/A	INTRINSIC
ZnF_C2H2	313	338	1.08e-1	SMART
ZnF_C2H2	344	368	7.15e-2	SMART
ZnF_C2H2	374	396	1.56e-2	SMART
ZnF_C2H2	402	424	2.61e-4	SMART
ZnF_C2H2	432	455	1.92e-2	SMART
low complexity region	459	471	N/A	INTRINSIC
low complexity region	491	502	N/A	INTRINSIC

Meta Mutation Damage Score

0.9713

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.7%

Validation Efficiency

100% (52/52)

MGI Phenotype

FUNCTION: The protein encoded by this gene is a member of the zinc finger family of proteins. The gene product contains C2H2-type domains, which are the classical zinc finger domains found in numerous nucleic acid-binding proteins. The homologous human protein has been shown to function as a regulator of the non-canonical NF-kappaB pathway in lymphotoxin-beta receptor signaling. A read-through transcript variant composed of Zfp91 and the downstream Cntf gene sequence has been identified, but it is thought to be non-coding. Read-through transcription of Zfp91 and Cntf has been observed in both human and mouse. A Zfp91-related pseudogene has also been identified on chromosome 17. [provided by RefSeq, Oct 2010]

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acvr1	T	A	2: 58,367,739 (GRCm39)	I141F	probably benign	Het
Adam6b	A	C	12: 113,454,281 (GRCm39)	Q366P	probably damaging	Het
Adck1	C	T	12: 88,397,920 (GRCm39)	Q185*	probably null	Het
Adgrb3	T	C	1: 25,870,108 (GRCm39)		probably benign	Het
Als2cl	T	C	9: 110,714,466 (GRCm39)	S105P	probably damaging	Het
Asns	C	A	6: 7,675,320 (GRCm39)	A561S	probably benign	Het
Bcl2a1d	A	T	9: 88,613,753 (GRCm39)	M7K	probably benign	Het
Bst1	A	T	5: 43,982,682 (GRCm39)		probably null	Het
Cdc37l1	T	C	19: 28,985,021 (GRCm39)	F224L	probably damaging	Het
Csmd3	G	A	15: 47,763,191 (GRCm39)	T1215I	possibly damaging	Het
Csnk1e	A	T	15: 79,305,129 (GRCm39)	S323T	probably benign	Het
Cyp2j7	G	T	4: 96,105,646 (GRCm39)	T288K	probably benign	Het
Dnaaf5	T	A	5: 139,149,147 (GRCm39)	I482N	probably damaging	Het
Dpy19l3	A	G	7: 35,392,284 (GRCm39)	I636T	probably damaging	Het
Enpep	T	G	3: 129,077,740 (GRCm39)	T626P	possibly damaging	Het
Fgfr2	G	A	7: 129,774,660 (GRCm39)	R509*	probably null	Het
Gm5849	T	A	3: 90,685,182 (GRCm39)	K1M	probably null	Het
Gpr83	T	C	9: 14,776,134 (GRCm39)	Y152H	probably damaging	Het
Hdac7	T	C	15: 97,705,596 (GRCm39)	E400G	probably damaging	Het
Hypk	A	T	2: 121,288,475 (GRCm39)	E82V	probably damaging	Het
Iqub	C	A	6: 24,505,867 (GRCm39)	A14S	probably benign	Het
Lias	T	C	5: 65,552,787 (GRCm39)	M130T	probably damaging	Het
Lingo4	T	C	3: 94,310,204 (GRCm39)	S381P	possibly damaging	Het
Mdn1	A	G	4: 32,704,635 (GRCm39)	T1536A	probably damaging	Het
Moxd2	T	A	6: 40,860,996 (GRCm39)	D265V	probably damaging	Het
Mtbp	C	A	15: 55,466,611 (GRCm39)	S541R	probably benign	Het
Or10j27	C	A	1: 172,957,869 (GRCm39)	C305F	probably benign	Het
Or2a54	T	C	6: 43,093,221 (GRCm39)	S182P	probably benign	Het
Or4k15b	T	C	14: 50,272,287 (GRCm39)	D191G	probably damaging	Het
Pcsk9	G	T	4: 106,316,156 (GRCm39)	Q96K	probably benign	Het
Pramel14	T	C	4: 143,718,192 (GRCm39)	Y417C	probably damaging	Het
Rln1	A	T	19: 29,311,962 (GRCm39)	F12Y	possibly damaging	Het
Setd1a	A	G	7: 127,384,903 (GRCm39)	N585D	possibly damaging	Het
Siah1b	G	A	X: 162,854,688 (GRCm39)	P131S	probably damaging	Het
Smarcal1	T	C	1: 72,650,637 (GRCm39)		probably benign	Het
Snx19	G	T	9: 30,339,895 (GRCm39)	L344F	probably benign	Het
Srfbp1	T	C	18: 52,621,403 (GRCm39)	C155R	probably benign	Het
Sspo	C	T	6: 48,464,287 (GRCm39)	R3914C	probably damaging	Het
Tacc2	G	A	7: 130,225,271 (GRCm39)	S652N	probably damaging	Het
Tmub1	A	C	5: 24,651,068 (GRCm39)	L197R	probably damaging	Het
Tnc	T	C	4: 63,926,066 (GRCm39)	T905A	possibly damaging	Het
Tnfrsf21	G	T	17: 43,398,733 (GRCm39)	R613L	possibly damaging	Het
Ugt3a1	A	T	15: 9,351,283 (GRCm39)	E97D	probably benign	Het
Vmn2r25	A	G	6: 123,816,756 (GRCm39)	I275T	probably benign	Het
Zfp1010	T	G	2: 176,956,889 (GRCm39)	Q203P	possibly damaging	Het
Zfp568	G	T	7: 29,721,721 (GRCm39)	C221F	probably benign	Het
Zswim3	T	A	2: 164,662,563 (GRCm39)	C348S	probably benign	Het

Other mutations in Zfp91

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02240:Zfp91	APN	19	12,753,770 (GRCm39)	splice site	probably benign
R0139:Zfp91	UTSW	19	12,747,834 (GRCm39)	missense	probably damaging	1.00
R0369:Zfp91	UTSW	19	12,747,438 (GRCm39)	missense	possibly damaging	0.95
R0485:Zfp91	UTSW	19	12,753,353 (GRCm39)	splice site	probably benign
R0559:Zfp91	UTSW	19	12,747,419 (GRCm39)	missense	probably damaging	1.00
R0597:Zfp91	UTSW	19	12,747,459 (GRCm39)	missense	possibly damaging	0.53
R1565:Zfp91	UTSW	19	12,756,439 (GRCm39)	missense	probably benign	0.02
R3422:Zfp91	UTSW	19	12,747,656 (GRCm39)	missense	probably benign	0.24
R4898:Zfp91	UTSW	19	12,747,424 (GRCm39)	missense	probably damaging	1.00
R4927:Zfp91	UTSW	19	12,753,774 (GRCm39)	critical splice donor site	probably null
R5509:Zfp91	UTSW	19	12,756,451 (GRCm39)	missense	probably damaging	0.99
R5878:Zfp91	UTSW	19	12,747,684 (GRCm39)	missense	possibly damaging	0.80
R5978:Zfp91	UTSW	19	12,747,515 (GRCm39)	missense	probably benign	0.43
R7703:Zfp91	UTSW	19	12,754,241 (GRCm39)	missense	probably benign	0.24
R7864:Zfp91	UTSW	19	12,748,403 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- GGTTTAATAGATCAAATGGAGGGCT -3'
(R):5'- TGGTACCATTGGACAATTATCTCTC -3'

Sequencing Primer
(F):5'- TGTATTTTTCACCAGCATGTA -3'
(R):5'- CGTAGATTGTAGTCCAACC -3'

Posted On

2015-07-21