Mutagenetix > Incidental Mutation

Incidental Mutation 'R4439:Zfp882'

329681

Institutional Source

Beutler Lab

Gene Symbol

Zfp882

Ensembl Gene

ENSMUSG00000089857

Gene Name

zinc finger protein 882

Synonyms

ENSMUSG00000052439

MMRRC Submission

041704-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.176) question?

Stock #

R4439 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

71908608-71916354 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 71913609 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 93 (F93L)

Ref Sequence

ENSEMBL: ENSMUSP00000105629 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000110002] [ENSMUST00000125802] [ENSMUST00000126607] [ENSMUST00000131544]

AlphaFold

E9Q4R4

Predicted Effect

probably damaging
Transcript: ENSMUST00000110002
AA Change: F93L

PolyPhen 2 Score 0.973 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000105629
Gene: ENSMUSG00000089857
AA Change: F93L

Domain	Start	End	E-Value	Type
KRAB	4	61	8.58e-14	SMART
ZnF_C2H2	84	106	1.47e-3	SMART
ZnF_C2H2	168	190	8.47e-4	SMART
ZnF_C2H2	196	218	2.27e-4	SMART
ZnF_C2H2	224	246	6.31e1	SMART
ZnF_C2H2	251	273	1.16e-1	SMART
ZnF_C2H2	279	301	1.25e-1	SMART
ZnF_C2H2	307	329	5.42e-2	SMART
ZnF_C2H2	335	357	1.47e-3	SMART
ZnF_C2H2	363	385	7.26e-3	SMART
ZnF_C2H2	391	413	1.26e-2	SMART
ZnF_C2H2	419	441	3.29e1	SMART
ZnF_C2H2	447	469	2.67e-1	SMART
ZnF_C2H2	475	497	1.04e-3	SMART
ZnF_C2H2	503	525	4.11e-2	SMART
ZnF_C2H2	531	553	4.87e-4	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000118290

Predicted Effect

probably benign
Transcript: ENSMUST00000125802

SMART Domains

Protein: ENSMUSP00000121316
Gene: ENSMUSG00000089857

Domain	Start	End	E-Value	Type
KRAB	12	69	8.58e-14	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000126607

SMART Domains

Protein: ENSMUSP00000119978
Gene: ENSMUSG00000089857

Domain	Start	End	E-Value	Type
KRAB	44	101	8.58e-14	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000131544

SMART Domains

Protein: ENSMUSP00000120213
Gene: ENSMUSG00000066880

Domain	Start	End	E-Value	Type
KRAB	4	56	1.08e-10	SMART
ZnF_C2H2	167	189	8.47e-4	SMART
ZnF_C2H2	195	217	8.34e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000170898

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.1%
20x: 94.8%

Validation Efficiency

94% (50/53)

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acacb	C	T	5: 114,246,496	T2237I	possibly damaging	Het
Adgre1	T	A	17: 57,447,954	L684Q	probably damaging	Het
Cfap157	T	A	2: 32,777,865	Y488F	probably benign	Het
D630045J12Rik	A	G	6: 38,194,761	I824T	probably benign	Het
Elmsan1	C	T	12: 84,156,471	G886S	probably benign	Het
Eno2	T	C	6: 124,762,959		probably benign	Het
Fam78b	C	A	1: 167,078,922	Q217K	probably damaging	Het
Fpgs	C	T	2: 32,687,501	C219Y	probably damaging	Het
Garem2	G	T	5: 30,113,346	V106L	possibly damaging	Het
Gm13084	A	T	4: 143,811,573	V276E	possibly damaging	Het
Grk3	A	T	5: 112,946,677		probably null	Het
Hint2	T	A	4: 43,654,919	Y70F	probably damaging	Het
Hist1h3b	A	T	13: 23,752,725		probably null	Het
Ipp	A	G	4: 116,515,077	N101S	probably benign	Het
Kcnj1	A	T	9: 32,394,118		probably benign	Het
Kcnk4	T	C	19: 6,932,761	D44G	probably benign	Het
Kif5c	T	C	2: 49,688,725	S122P	possibly damaging	Het
Nipbl	T	A	15: 8,338,724	K1171N	probably damaging	Het
Olfr1285	T	A	2: 111,409,308		noncoding transcript	Het
Pcf11	A	C	7: 92,658,017	L981R	probably damaging	Het
Pias2	T	A	18: 77,097,703	L153H	probably damaging	Het
Pjvk	T	C	2: 76,651,406	S68P	probably damaging	Het
Pkd1	T	C	17: 24,585,692	V3130A	probably damaging	Het
Plpp4	C	A	7: 129,257,089		probably benign	Het
Plxnd1	T	A	6: 115,993,976	H277L	probably damaging	Het
Rasgrf2	T	C	13: 91,983,678	D620G	possibly damaging	Het
Rd3l	C	A	12: 111,979,658	S63I	possibly damaging	Het
Scfd2	G	C	5: 74,397,707	A503G	possibly damaging	Het
Slco4a1	T	C	2: 180,472,662	V549A	probably benign	Het
Tenm4	A	G	7: 96,895,815	N2375S	probably benign	Het
Tespa1	C	T	10: 130,361,957	R283C	probably damaging	Het
Tle2	A	G	10: 81,581,682	E227G	possibly damaging	Het
Tnn	T	G	1: 160,116,080	E1054D	probably benign	Het
Tnrc6a	A	T	7: 123,152,182	K54*	probably null	Het
Tns2	C	T	15: 102,108,934	R281C	probably damaging	Het
Tpr	T	A	1: 150,403,961	D206E	probably benign	Het
Ugt2b34	A	T	5: 86,892,867	F399I	probably damaging	Het
Usp3	A	T	9: 66,518,494	D456E	probably benign	Het
Vmn1r60	T	A	7: 5,544,489	H204L	probably damaging	Het
Vmn2r97	G	T	17: 18,930,354	A488S	probably benign	Het
Vrk3	C	T	7: 44,775,442	T427M	probably benign	Het
Wdr86	T	C	5: 24,730,237	D36G	probably damaging	Het
Zc3h4	C	T	7: 16,429,111	P479S	unknown	Het
Zfhx4	A	T	3: 5,214,815		probably benign	Het
Zfp607b	T	A	7: 27,702,724	C202S	probably damaging	Het

Other mutations in Zfp882

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00676:Zfp882	APN	8	71913827	missense	probably benign
R0244:Zfp882	UTSW	8	71913523	missense	possibly damaging	0.79
R0270:Zfp882	UTSW	8	71914615	missense	probably benign	0.05
R0636:Zfp882	UTSW	8	71914337	missense	probably benign	0.01
R0840:Zfp882	UTSW	8	71914686	nonsense	probably null
R1299:Zfp882	UTSW	8	71913473	missense	probably damaging	1.00
R4829:Zfp882	UTSW	8	71914389	missense	probably damaging	1.00
R5028:Zfp882	UTSW	8	71914654	missense	possibly damaging	0.70
R5296:Zfp882	UTSW	8	71914360	missense	probably damaging	1.00
R5882:Zfp882	UTSW	8	71913459	critical splice acceptor site	probably null
R5974:Zfp882	UTSW	8	71913155	missense	probably damaging	1.00
R6052:Zfp882	UTSW	8	71914505	missense	probably benign	0.01
R6383:Zfp882	UTSW	8	71914640	missense	probably damaging	1.00
R6888:Zfp882	UTSW	8	71914286	missense	probably benign	0.01
R6987:Zfp882	UTSW	8	71914673	missense	probably benign	0.01
R7045:Zfp882	UTSW	8	71913249	critical splice donor site	probably null
R7780:Zfp882	UTSW	8	71914229	missense	possibly damaging	0.89
R7793:Zfp882	UTSW	8	71913141	missense	probably damaging	1.00
R8386:Zfp882	UTSW	8	71914118	missense	probably benign	0.00
R9452:Zfp882	UTSW	8	71914987	missense	probably damaging	1.00
R9694:Zfp882	UTSW	8	71914071	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CCAGTCATGATCTTATACCAGAAAC -3'
(R):5'- GCAAGAGTCAAAGAGTCTTTCC -3'

Sequencing Primer
(F):5'- GATCCTTGAAGATGGCTC -3'
(R):5'- GAGTCAAAGAGTCTTTCCCACTTTG -3'

Posted On

2015-07-21