Mutagenetix > Incidental Mutation

Incidental Mutation 'R4439:Vmn2r97'

329694

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r97

Ensembl Gene

ENSMUSG00000091491

Gene Name

vomeronasal 2, receptor 97

Synonyms

EG627367

MMRRC Submission

041704-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.071) question?

Stock #

R4439 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

19134584-19168333 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 19150616 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Serine at position 488 (A488S)

Ref Sequence

ENSEMBL: ENSMUSP00000156141 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168710] [ENSMUST00000232219]

AlphaFold

K7N6Z2

Predicted Effect

probably benign
Transcript: ENSMUST00000168710
AA Change: A488S

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000129313
Gene: ENSMUSG00000091491
AA Change: A488S

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	82	442	2.9e-36	PFAM
Pfam:NCD3G	513	566	4.9e-21	PFAM
Pfam:7tm_3	599	834	1.7e-52	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000232219
AA Change: A488S

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000232325

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.1%
20x: 94.8%

Validation Efficiency

94% (50/53)

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acacb	C	T	5: 114,384,557 (GRCm39)	T2237I	possibly damaging	Het
Adgre1	T	A	17: 57,754,954 (GRCm39)	L684Q	probably damaging	Het
Cfap157	T	A	2: 32,667,877 (GRCm39)	Y488F	probably benign	Het
D630045J12Rik	A	G	6: 38,171,696 (GRCm39)	I824T	probably benign	Het
Eno2	T	C	6: 124,739,922 (GRCm39)		probably benign	Het
Fam78b	C	A	1: 166,906,491 (GRCm39)	Q217K	probably damaging	Het
Fpgs	C	T	2: 32,577,513 (GRCm39)	C219Y	probably damaging	Het
Garem2	G	T	5: 30,318,344 (GRCm39)	V106L	possibly damaging	Het
Grk3	A	T	5: 113,094,543 (GRCm39)		probably null	Het
H3c2	A	T	13: 23,936,708 (GRCm39)		probably null	Het
Hint2	T	A	4: 43,654,919 (GRCm39)	Y70F	probably damaging	Het
Ipp	A	G	4: 116,372,274 (GRCm39)	N101S	probably benign	Het
Kcnj1	A	T	9: 32,305,414 (GRCm39)		probably benign	Het
Kcnk4	T	C	19: 6,910,129 (GRCm39)	D44G	probably benign	Het
Kif5c	T	C	2: 49,578,737 (GRCm39)	S122P	possibly damaging	Het
Mideas	C	T	12: 84,203,245 (GRCm39)	G886S	probably benign	Het
Nipbl	T	A	15: 8,368,208 (GRCm39)	K1171N	probably damaging	Het
Or4k39	T	A	2: 111,239,653 (GRCm39)		noncoding transcript	Het
Pcf11	A	C	7: 92,307,225 (GRCm39)	L981R	probably damaging	Het
Pias2	T	A	18: 77,185,399 (GRCm39)	L153H	probably damaging	Het
Pjvk	T	C	2: 76,481,750 (GRCm39)	S68P	probably damaging	Het
Pkd1	T	C	17: 24,804,666 (GRCm39)	V3130A	probably damaging	Het
Plpp4	C	A	7: 128,858,813 (GRCm39)		probably benign	Het
Plxnd1	T	A	6: 115,970,937 (GRCm39)	H277L	probably damaging	Het
Pramel26	A	T	4: 143,538,143 (GRCm39)	V276E	possibly damaging	Het
Rasgrf2	T	C	13: 92,131,797 (GRCm39)	D620G	possibly damaging	Het
Rd3l	C	A	12: 111,946,092 (GRCm39)	S63I	possibly damaging	Het
Scfd2	G	C	5: 74,558,368 (GRCm39)	A503G	possibly damaging	Het
Slco4a1	T	C	2: 180,114,455 (GRCm39)	V549A	probably benign	Het
Tenm4	A	G	7: 96,545,022 (GRCm39)	N2375S	probably benign	Het
Tespa1	C	T	10: 130,197,826 (GRCm39)	R283C	probably damaging	Het
Tle2	A	G	10: 81,417,516 (GRCm39)	E227G	possibly damaging	Het
Tnn	T	G	1: 159,943,650 (GRCm39)	E1054D	probably benign	Het
Tnrc6a	A	T	7: 122,751,405 (GRCm39)	K54*	probably null	Het
Tns2	C	T	15: 102,017,369 (GRCm39)	R281C	probably damaging	Het
Tpr	T	A	1: 150,279,712 (GRCm39)	D206E	probably benign	Het
Ugt2b34	A	T	5: 87,040,726 (GRCm39)	F399I	probably damaging	Het
Usp3	A	T	9: 66,425,776 (GRCm39)	D456E	probably benign	Het
Vmn1r60	T	A	7: 5,547,488 (GRCm39)	H204L	probably damaging	Het
Vrk3	C	T	7: 44,424,866 (GRCm39)	T427M	probably benign	Het
Wdr86	T	C	5: 24,935,235 (GRCm39)	D36G	probably damaging	Het
Zc3h4	C	T	7: 16,163,036 (GRCm39)	P479S	unknown	Het
Zfhx4	A	T	3: 5,279,875 (GRCm39)		probably benign	Het
Zfp607b	T	A	7: 27,402,149 (GRCm39)	C202S	probably damaging	Het
Zfp882	T	A	8: 72,667,453 (GRCm39)	F93L	probably damaging	Het

Other mutations in Vmn2r97

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00897:Vmn2r97	APN	17	19,167,921 (GRCm39)	missense	probably benign	0.37
IGL00962:Vmn2r97	APN	17	19,149,490 (GRCm39)	missense	probably damaging	1.00
IGL01704:Vmn2r97	APN	17	19,168,073 (GRCm39)	missense	probably damaging	0.99
IGL01888:Vmn2r97	APN	17	19,149,286 (GRCm39)	nonsense	probably null
IGL02429:Vmn2r97	APN	17	19,150,596 (GRCm39)	missense	possibly damaging	0.94
IGL02742:Vmn2r97	APN	17	19,149,432 (GRCm39)	missense	probably damaging	0.97
IGL02934:Vmn2r97	APN	17	19,149,947 (GRCm39)	missense	probably benign	0.00
IGL02978:Vmn2r97	APN	17	19,168,298 (GRCm39)	missense	probably benign	0.01
IGL03230:Vmn2r97	APN	17	19,149,668 (GRCm39)	missense	probably benign	0.10
IGL03241:Vmn2r97	APN	17	19,148,438 (GRCm39)	missense	probably benign	0.11
IGL03050:Vmn2r97	UTSW	17	19,167,900 (GRCm39)	missense	possibly damaging	0.84
PIT4469001:Vmn2r97	UTSW	17	19,149,878 (GRCm39)	missense	probably benign	0.00
R0482:Vmn2r97	UTSW	17	19,167,930 (GRCm39)	missense	probably damaging	1.00
R0514:Vmn2r97	UTSW	17	19,134,734 (GRCm39)	missense	probably benign	0.25
R0944:Vmn2r97	UTSW	17	19,167,665 (GRCm39)	missense	probably benign	0.13
R1061:Vmn2r97	UTSW	17	19,148,440 (GRCm39)	nonsense	probably null
R1546:Vmn2r97	UTSW	17	19,168,110 (GRCm39)	missense	probably damaging	1.00
R1725:Vmn2r97	UTSW	17	19,149,397 (GRCm39)	missense	probably benign	0.43
R1860:Vmn2r97	UTSW	17	19,167,648 (GRCm39)	missense	probably benign	0.01
R1938:Vmn2r97	UTSW	17	19,149,593 (GRCm39)	missense	probably benign	0.01
R1944:Vmn2r97	UTSW	17	19,160,500 (GRCm39)	missense	probably benign	0.00
R2027:Vmn2r97	UTSW	17	19,149,944 (GRCm39)	missense	unknown
R2106:Vmn2r97	UTSW	17	19,168,100 (GRCm39)	missense	probably damaging	1.00
R2151:Vmn2r97	UTSW	17	19,167,584 (GRCm39)	nonsense	probably null
R2153:Vmn2r97	UTSW	17	19,167,584 (GRCm39)	nonsense	probably null
R2154:Vmn2r97	UTSW	17	19,167,584 (GRCm39)	nonsense	probably null
R2516:Vmn2r97	UTSW	17	19,167,814 (GRCm39)	missense	probably benign
R3739:Vmn2r97	UTSW	17	19,148,413 (GRCm39)	missense	probably damaging	1.00
R3744:Vmn2r97	UTSW	17	19,149,890 (GRCm39)	missense	probably benign
R3885:Vmn2r97	UTSW	17	19,148,596 (GRCm39)	missense	possibly damaging	0.90
R3899:Vmn2r97	UTSW	17	19,167,873 (GRCm39)	missense	probably damaging	0.96
R4115:Vmn2r97	UTSW	17	19,148,332 (GRCm39)	missense	probably benign	0.01
R4247:Vmn2r97	UTSW	17	19,167,542 (GRCm39)	missense	possibly damaging	0.83
R4287:Vmn2r97	UTSW	17	19,168,337 (GRCm39)	intron	probably benign
R4523:Vmn2r97	UTSW	17	19,149,333 (GRCm39)	missense	probably benign	0.03
R4783:Vmn2r97	UTSW	17	19,149,550 (GRCm39)	missense	probably benign
R4948:Vmn2r97	UTSW	17	19,167,561 (GRCm39)	missense	possibly damaging	0.95
R4981:Vmn2r97	UTSW	17	19,160,436 (GRCm39)	nonsense	probably null
R5029:Vmn2r97	UTSW	17	19,168,173 (GRCm39)	missense	probably damaging	1.00
R5200:Vmn2r97	UTSW	17	19,148,615 (GRCm39)	missense	probably damaging	1.00
R5541:Vmn2r97	UTSW	17	19,148,617 (GRCm39)	nonsense	probably null
R5637:Vmn2r97	UTSW	17	19,167,628 (GRCm39)	nonsense	probably null
R5765:Vmn2r97	UTSW	17	19,167,442 (GRCm39)	nonsense	probably null
R5885:Vmn2r97	UTSW	17	19,168,035 (GRCm39)	missense	possibly damaging	0.50
R6272:Vmn2r97	UTSW	17	19,167,861 (GRCm39)	missense	possibly damaging	0.70
R6553:Vmn2r97	UTSW	17	19,150,566 (GRCm39)	nonsense	probably null
R6818:Vmn2r97	UTSW	17	19,168,193 (GRCm39)	missense	possibly damaging	0.95
R6880:Vmn2r97	UTSW	17	19,134,770 (GRCm39)	missense	probably benign	0.00
R7012:Vmn2r97	UTSW	17	19,167,756 (GRCm39)	missense	probably damaging	0.99
R7023:Vmn2r97	UTSW	17	19,134,663 (GRCm39)	missense	probably damaging	1.00
R7044:Vmn2r97	UTSW	17	19,134,629 (GRCm39)	missense	probably benign	0.05
R7191:Vmn2r97	UTSW	17	19,150,548 (GRCm39)	missense	probably damaging	1.00
R7503:Vmn2r97	UTSW	17	19,148,470 (GRCm39)	missense	probably benign
R7862:Vmn2r97	UTSW	17	19,167,416 (GRCm39)	missense	probably damaging	1.00
R7876:Vmn2r97	UTSW	17	19,149,326 (GRCm39)	missense	probably damaging	0.97
R7890:Vmn2r97	UTSW	17	19,149,802 (GRCm39)	missense	probably benign	0.00
R7936:Vmn2r97	UTSW	17	19,150,662 (GRCm39)	missense	probably damaging	1.00
R7978:Vmn2r97	UTSW	17	19,167,854 (GRCm39)	missense	probably damaging	1.00
R8405:Vmn2r97	UTSW	17	19,134,802 (GRCm39)	critical splice donor site	probably null
R8755:Vmn2r97	UTSW	17	19,168,104 (GRCm39)	missense	probably damaging	1.00
R8790:Vmn2r97	UTSW	17	19,160,472 (GRCm39)	missense	probably damaging	1.00
R8850:Vmn2r97	UTSW	17	19,149,607 (GRCm39)	missense	probably benign	0.00
R9060:Vmn2r97	UTSW	17	19,134,585 (GRCm39)	start codon destroyed	probably null	0.94
R9079:Vmn2r97	UTSW	17	19,149,640 (GRCm39)	missense	probably benign
R9252:Vmn2r97	UTSW	17	19,167,849 (GRCm39)	missense	probably benign	0.00
R9278:Vmn2r97	UTSW	17	19,134,762 (GRCm39)	missense	probably benign	0.00
R9342:Vmn2r97	UTSW	17	19,149,368 (GRCm39)	missense	probably benign
R9422:Vmn2r97	UTSW	17	19,149,333 (GRCm39)	missense	probably benign	0.03
R9496:Vmn2r97	UTSW	17	19,149,227 (GRCm39)	missense	probably damaging	1.00
R9571:Vmn2r97	UTSW	17	19,149,919 (GRCm39)	missense	probably benign
R9601:Vmn2r97	UTSW	17	19,134,770 (GRCm39)	missense	probably benign
R9672:Vmn2r97	UTSW	17	19,149,442 (GRCm39)	missense	probably benign	0.00
R9773:Vmn2r97	UTSW	17	19,168,221 (GRCm39)	missense	probably benign	0.01
R9795:Vmn2r97	UTSW	17	19,167,561 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGGTTCTACGTTTTCACTTGAAGAC -3'
(R):5'- TCCTCATATTAGTGAACAATGGGG -3'

Sequencing Primer
(F):5'- GCTATTCTGTATAGTCAGGTTTTCAC -3'
(R):5'- CAATGGGGGATTTATTGAACACTG -3'

Posted On

2015-07-21