Incidental Mutation 'R4464:Trim66'
ID 330272
Institutional Source Beutler Lab
Gene Symbol Trim66
Ensembl Gene ENSMUSG00000031026
Gene Name tripartite motif-containing 66
Synonyms Tif1d, D7H11orf29
MMRRC Submission 041722-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.226) question?
Stock # R4464 (G1)
Quality Score 225
Status Validated
Chromosome 7
Chromosomal Location 109449006-109508134 bp(-) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) A to T at 109477690 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Serine to Arginine at position 347 (S347R)
Ref Sequence ENSEMBL: ENSMUSP00000102352 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000033339] [ENSMUST00000106739] [ENSMUST00000106741]
AlphaFold Q924W6
Predicted Effect probably benign
Transcript: ENSMUST00000033339
AA Change: S245R

PolyPhen 2 Score 0.369 (Sensitivity: 0.90; Specificity: 0.89)
SMART Domains Protein: ENSMUSP00000033339
Gene: ENSMUSG00000031026
AA Change: S245R

DomainStartEndE-ValueType
PHD 4 69 7.77e0 SMART
BBC 108 234 1.61e-39 SMART
low complexity region 318 333 N/A INTRINSIC
low complexity region 452 486 N/A INTRINSIC
low complexity region 517 530 N/A INTRINSIC
low complexity region 568 581 N/A INTRINSIC
PHD 998 1041 4.09e-10 SMART
BROMO 1069 1175 8.22e-27 SMART
low complexity region 1185 1199 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000106739
AA Change: S245R

PolyPhen 2 Score 0.369 (Sensitivity: 0.90; Specificity: 0.89)
SMART Domains Protein: ENSMUSP00000102350
Gene: ENSMUSG00000031026
AA Change: S245R

DomainStartEndE-ValueType
PHD 4 69 7.77e0 SMART
BBC 108 234 1.61e-39 SMART
low complexity region 318 333 N/A INTRINSIC
low complexity region 452 486 N/A INTRINSIC
low complexity region 517 530 N/A INTRINSIC
low complexity region 568 581 N/A INTRINSIC
PHD 998 1041 4.09e-10 SMART
BROMO 1069 1175 8.22e-27 SMART
low complexity region 1185 1199 N/A INTRINSIC
Predicted Effect possibly damaging
Transcript: ENSMUST00000106741
AA Change: S347R

PolyPhen 2 Score 0.511 (Sensitivity: 0.88; Specificity: 0.90)
SMART Domains Protein: ENSMUSP00000102352
Gene: ENSMUSG00000031026
AA Change: S347R

DomainStartEndE-ValueType
RING 28 78 2.38e-2 SMART
BBOX 102 140 1.48e0 SMART
PHD 106 171 7.77e0 SMART
RING 107 170 4.38e0 SMART
BBOX 162 203 4.21e-3 SMART
BBC 210 336 1.61e-39 SMART
low complexity region 420 435 N/A INTRINSIC
low complexity region 554 588 N/A INTRINSIC
low complexity region 619 632 N/A INTRINSIC
low complexity region 670 683 N/A INTRINSIC
PHD 1100 1143 4.09e-10 SMART
BROMO 1171 1277 8.22e-27 SMART
low complexity region 1287 1301 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000137704
Meta Mutation Damage Score 0.0801 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.5%
  • 10x: 97.1%
  • 20x: 94.8%
Validation Efficiency 97% (35/36)
Allele List at MGI
Other mutations in this stock
Total: 31 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
0610040J01Rik G T 5: 63,898,839 (GRCm38) probably benign Het
Abcc3 A G 11: 94,358,786 (GRCm38) V1111A probably benign Het
Acot10 G A 15: 20,665,744 (GRCm38) R304* probably null Het
Aldh8a1 C A 10: 21,388,941 (GRCm38) probably benign Het
Alms1 A G 6: 85,620,021 (GRCm38) T1079A possibly damaging Het
Armc3 T C 2: 19,248,659 (GRCm38) Y204H probably damaging Het
Asnsd1 C A 1: 53,352,527 (GRCm38) probably null Het
Atad5 T A 11: 80,100,311 (GRCm38) probably null Het
Cst12 G A 2: 148,789,517 (GRCm38) V53I possibly damaging Het
Cylc2 C G 4: 51,229,651 (GRCm38) T331R unknown Het
Dennd1a A T 2: 38,243,390 (GRCm38) probably benign Het
Fam213a T A 14: 40,997,875 (GRCm38) K127N probably damaging Het
Gm7535 C A 17: 17,911,662 (GRCm38) probably benign Het
Gpr158 T A 2: 21,826,999 (GRCm38) M970K probably damaging Het
Ifngr1 G A 10: 19,597,517 (GRCm38) V72I possibly damaging Het
Kifap3 C A 1: 163,817,895 (GRCm38) Q269K probably benign Het
Krt86 G A 15: 101,473,914 (GRCm38) D122N probably damaging Het
Lrrcc1 A G 3: 14,557,318 (GRCm38) K694E probably damaging Het
Mbd4 A G 6: 115,849,502 (GRCm38) L155S probably damaging Het
Nalcn T C 14: 123,323,350 (GRCm38) N772D probably benign Het
Olfr109 C T 17: 37,466,851 (GRCm38) S215F probably damaging Het
Psg29 A T 7: 17,210,650 (GRCm38) N362Y possibly damaging Het
Ptpn23 G A 9: 110,386,813 (GRCm38) T1325I probably damaging Het
Rad51ap1 T C 6: 126,934,768 (GRCm38) N52S possibly damaging Het
Rb1 C A 14: 73,199,198 (GRCm38) probably null Het
Slc34a2 T C 5: 53,069,182 (GRCm38) L490P probably damaging Het
Sost G A 11: 101,966,844 (GRCm38) P44S probably damaging Het
St3gal2 A G 8: 110,967,502 (GRCm38) N207D probably benign Het
Stat1 T G 1: 52,137,416 (GRCm38) D257E possibly damaging Het
Tkt A G 14: 30,568,274 (GRCm38) T165A possibly damaging Het
Zfp429 T C 13: 67,390,498 (GRCm38) I276V probably benign Het
Other mutations in Trim66
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01539:Trim66 APN 7 109,455,066 (GRCm38) missense probably benign 0.02
IGL01758:Trim66 APN 7 109,486,045 (GRCm38) critical splice donor site probably null
IGL01982:Trim66 APN 7 109,458,763 (GRCm38) missense probably benign 0.00
IGL01983:Trim66 APN 7 109,458,251 (GRCm38) nonsense probably null
IGL02149:Trim66 APN 7 109,460,902 (GRCm38) missense possibly damaging 0.66
IGL02392:Trim66 APN 7 109,460,274 (GRCm38) missense probably benign 0.01
IGL02483:Trim66 APN 7 109,477,630 (GRCm38) splice site probably benign
IGL02832:Trim66 APN 7 109,460,497 (GRCm38) missense probably damaging 1.00
IGL02945:Trim66 APN 7 109,460,176 (GRCm38) nonsense probably null
IGL03085:Trim66 APN 7 109,458,745 (GRCm38) missense probably benign 0.17
PIT1430001:Trim66 UTSW 7 109,475,247 (GRCm38) missense probably damaging 0.99
R0326:Trim66 UTSW 7 109,460,172 (GRCm38) missense probably benign 0.00
R0358:Trim66 UTSW 7 109,460,176 (GRCm38) nonsense probably null
R0401:Trim66 UTSW 7 109,475,264 (GRCm38) missense probably damaging 0.98
R0470:Trim66 UTSW 7 109,457,542 (GRCm38) splice site probably benign
R0568:Trim66 UTSW 7 109,460,695 (GRCm38) missense probably benign 0.00
R0669:Trim66 UTSW 7 109,454,992 (GRCm38) intron probably benign
R0980:Trim66 UTSW 7 109,455,670 (GRCm38) missense probably damaging 1.00
R1015:Trim66 UTSW 7 109,455,233 (GRCm38) missense probably damaging 1.00
R1078:Trim66 UTSW 7 109,472,319 (GRCm38) missense probably damaging 1.00
R1099:Trim66 UTSW 7 109,475,454 (GRCm38) missense probably benign 0.34
R1181:Trim66 UTSW 7 109,484,577 (GRCm38) critical splice donor site probably null
R1497:Trim66 UTSW 7 109,484,619 (GRCm38) missense probably benign 0.00
R1583:Trim66 UTSW 7 109,455,080 (GRCm38) missense probably damaging 1.00
R1843:Trim66 UTSW 7 109,475,839 (GRCm38) missense probably damaging 0.99
R1998:Trim66 UTSW 7 109,484,577 (GRCm38) critical splice donor site probably null
R2016:Trim66 UTSW 7 109,472,232 (GRCm38) critical splice donor site probably null
R2143:Trim66 UTSW 7 109,475,113 (GRCm38) missense probably damaging 0.98
R2144:Trim66 UTSW 7 109,475,113 (GRCm38) missense probably damaging 0.98
R2145:Trim66 UTSW 7 109,475,113 (GRCm38) missense probably damaging 0.98
R3945:Trim66 UTSW 7 109,472,268 (GRCm38) missense possibly damaging 0.94
R4012:Trim66 UTSW 7 109,458,131 (GRCm38) missense probably damaging 0.98
R4473:Trim66 UTSW 7 109,481,995 (GRCm38) missense probably damaging 1.00
R4729:Trim66 UTSW 7 109,456,060 (GRCm38) critical splice donor site probably null
R4730:Trim66 UTSW 7 109,483,069 (GRCm38) missense probably damaging 1.00
R4775:Trim66 UTSW 7 109,457,589 (GRCm38) nonsense probably null
R4819:Trim66 UTSW 7 109,457,586 (GRCm38) missense probably damaging 1.00
R5269:Trim66 UTSW 7 109,457,590 (GRCm38) missense probably benign 0.00
R5557:Trim66 UTSW 7 109,483,737 (GRCm38) missense probably benign 0.06
R5832:Trim66 UTSW 7 109,455,202 (GRCm38) missense probably damaging 1.00
R6220:Trim66 UTSW 7 109,483,093 (GRCm38) missense probably damaging 0.97
R6243:Trim66 UTSW 7 109,460,274 (GRCm38) missense probably benign 0.01
R6374:Trim66 UTSW 7 109,486,062 (GRCm38) missense probably benign
R6450:Trim66 UTSW 7 109,460,738 (GRCm38) missense probably benign 0.09
R6543:Trim66 UTSW 7 109,475,879 (GRCm38) missense probably benign 0.01
R6788:Trim66 UTSW 7 109,477,754 (GRCm38) missense probably damaging 1.00
R6842:Trim66 UTSW 7 109,460,776 (GRCm38) missense probably benign 0.00
R7169:Trim66 UTSW 7 109,455,121 (GRCm38) missense probably benign 0.25
R7257:Trim66 UTSW 7 109,460,244 (GRCm38) missense probably damaging 1.00
R7328:Trim66 UTSW 7 109,457,751 (GRCm38) missense probably damaging 0.99
R7616:Trim66 UTSW 7 109,483,749 (GRCm38) missense probably damaging 0.99
R8423:Trim66 UTSW 7 109,475,392 (GRCm38) missense possibly damaging 0.77
R8855:Trim66 UTSW 7 109,481,981 (GRCm38) missense probably damaging 1.00
R9130:Trim66 UTSW 7 109,477,689 (GRCm38) missense possibly damaging 0.90
R9137:Trim66 UTSW 7 109,475,123 (GRCm38) missense probably damaging 0.99
R9640:Trim66 UTSW 7 109,475,618 (GRCm38) missense probably damaging 1.00
RF013:Trim66 UTSW 7 109,460,753 (GRCm38) missense probably damaging 0.99
RF024:Trim66 UTSW 7 109,460,740 (GRCm38) missense possibly damaging 0.62
Predicted Primers PCR Primer
(F):5'- TGACTGGCTCCATGATCAATG -3'
(R):5'- AACACTGTCTGGGAAGACTG -3'

Sequencing Primer
(F):5'- CAATGGCAGTCTGAGAGCACTTTC -3'
(R):5'- TCCTGGGGACATGGAACTG -3'
Posted On 2015-07-21