Mutagenetix > Incidental Mutations

Incidental Mutation 'R4464:Zfp429'

330280

Institutional Source

Beutler Lab

Gene Symbol

Zfp429

Ensembl Gene

ENSMUSG00000078994

Gene Name

zinc finger protein 429

Synonyms

2810487A22Rik

MMRRC Submission

041722-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.078) question?

Stock #

R4464 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

67387905-67399819 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 67390498 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 276 (I276V)

Ref Sequence

ENSEMBL: ENSMUSP00000105354 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109732] [ENSMUST00000181071] [ENSMUST00000224684] [ENSMUST00000224825]

AlphaFold

Q7M6Y0

Predicted Effect

probably benign
Transcript: ENSMUST00000109732
AA Change: I276V

PolyPhen 2 Score 0.132 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000105354
Gene: ENSMUSG00000078994
AA Change: I276V

Domain	Start	End	E-Value	Type
KRAB	15	75	7.16e-34	SMART
ZnF_C2H2	119	141	5.12e1	SMART
ZnF_C2H2	147	169	2.27e-4	SMART
ZnF_C2H2	175	197	1.28e-3	SMART
ZnF_C2H2	203	225	1.56e-2	SMART
ZnF_C2H2	259	281	4.62e1	SMART
ZnF_C2H2	287	309	5.14e-3	SMART
ZnF_C2H2	315	337	6.78e-3	SMART
ZnF_C2H2	343	365	3.11e-2	SMART
ZnF_C2H2	371	393	1.25e-1	SMART
ZnF_C2H2	399	421	6.32e-3	SMART
ZnF_C2H2	427	449	1.47e-3	SMART
ZnF_C2H2	455	477	5.42e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000181071

SMART Domains

Protein: ENSMUSP00000137755
Gene: ENSMUSG00000078994

Domain	Start	End	E-Value	Type
KRAB	15	75	7.16e-34	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000224684

Predicted Effect

probably benign
Transcript: ENSMUST00000224825

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000225810

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.1%
20x: 94.8%

Validation Efficiency

97% (35/36)

Allele List at MGI

Other mutations in this stock

Total: 31 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610040J01Rik	G	T	5: 63,898,839		probably benign	Het
Abcc3	A	G	11: 94,358,786	V1111A	probably benign	Het
Acot10	G	A	15: 20,665,744	R304*	probably null	Het
Aldh8a1	C	A	10: 21,388,941		probably benign	Het
Alms1	A	G	6: 85,620,021	T1079A	possibly damaging	Het
Armc3	T	C	2: 19,248,659	Y204H	probably damaging	Het
Asnsd1	C	A	1: 53,352,527		probably null	Het
Atad5	T	A	11: 80,100,311		probably null	Het
Cst12	G	A	2: 148,789,517	V53I	possibly damaging	Het
Cylc2	C	G	4: 51,229,651	T331R	unknown	Het
Dennd1a	A	T	2: 38,243,390		probably benign	Het
Fam213a	T	A	14: 40,997,875	K127N	probably damaging	Het
Gm7535	C	A	17: 17,911,662		probably benign	Het
Gpr158	T	A	2: 21,826,999	M970K	probably damaging	Het
Ifngr1	G	A	10: 19,597,517	V72I	possibly damaging	Het
Kifap3	C	A	1: 163,817,895	Q269K	probably benign	Het
Krt86	G	A	15: 101,473,914	D122N	probably damaging	Het
Lrrcc1	A	G	3: 14,557,318	K694E	probably damaging	Het
Mbd4	A	G	6: 115,849,502	L155S	probably damaging	Het
Nalcn	T	C	14: 123,323,350	N772D	probably benign	Het
Olfr109	C	T	17: 37,466,851	S215F	probably damaging	Het
Psg29	A	T	7: 17,210,650	N362Y	possibly damaging	Het
Ptpn23	G	A	9: 110,386,813	T1325I	probably damaging	Het
Rad51ap1	T	C	6: 126,934,768	N52S	possibly damaging	Het
Rb1	C	A	14: 73,199,198		probably null	Het
Slc34a2	T	C	5: 53,069,182	L490P	probably damaging	Het
Sost	G	A	11: 101,966,844	P44S	probably damaging	Het
St3gal2	A	G	8: 110,967,502	N207D	probably benign	Het
Stat1	T	G	1: 52,137,416	D257E	possibly damaging	Het
Tkt	A	G	14: 30,568,274	T165A	possibly damaging	Het
Trim66	A	T	7: 109,477,690	S347R	possibly damaging	Het

Other mutations in Zfp429

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01160:Zfp429	APN	13	67391013	missense	probably damaging	0.96
IGL01913:Zfp429	APN	13	67396674	missense	probably damaging	1.00
IGL02343:Zfp429	APN	13	67390725	missense	probably damaging	0.98
IGL02679:Zfp429	APN	13	67399736	intron	probably benign
IGL03396:Zfp429	APN	13	67396040	splice site	probably benign
FR4342:Zfp429	UTSW	13	67396650	missense	probably benign	0.02
R0012:Zfp429	UTSW	13	67390677	missense	probably benign	0.01
R1232:Zfp429	UTSW	13	67390632	missense	possibly damaging	0.47
R1330:Zfp429	UTSW	13	67396143	splice site	probably null
R1653:Zfp429	UTSW	13	67389924	missense	possibly damaging	0.87
R1761:Zfp429	UTSW	13	67396076	missense	probably benign	0.28
R1813:Zfp429	UTSW	13	67390386	missense	possibly damaging	0.55
R2356:Zfp429	UTSW	13	67390627	missense	probably benign
R4280:Zfp429	UTSW	13	67390795	missense	probably damaging	1.00
R4283:Zfp429	UTSW	13	67390795	missense	probably damaging	1.00
R4789:Zfp429	UTSW	13	67390404	missense	probably benign	0.06
R5187:Zfp429	UTSW	13	67390840	missense	probably damaging	0.99
R5250:Zfp429	UTSW	13	67390519	missense	probably benign	0.00
R6688:Zfp429	UTSW	13	67396130	missense	probably damaging	0.98
R6772:Zfp429	UTSW	13	67390198	missense	probably damaging	1.00
R6989:Zfp429	UTSW	13	67389961	missense	probably benign	0.00
R7041:Zfp429	UTSW	13	67390711	missense	probably damaging	1.00
R7101:Zfp429	UTSW	13	67390812	missense	possibly damaging	0.88
R7593:Zfp429	UTSW	13	67390291	missense	probably damaging	1.00
R7792:Zfp429	UTSW	13	67390439	nonsense	probably null
R8500:Zfp429	UTSW	13	67390709	nonsense	probably null
R8721:Zfp429	UTSW	13	67390212	missense	probably damaging	0.98
R8891:Zfp429	UTSW	13	67390711	missense	probably damaging	1.00
R9364:Zfp429	UTSW	13	67390412	missense	probably benign	0.12

Predicted Primers

PCR Primer
(F):5'- TGTCTTTTAAGGCAGGAAGATGAGG -3'
(R):5'- GGCCTTCAGTACTCGCTCAT -3'

Sequencing Primer
(F):5'- GGAATAGCATTTGCCACAGTC -3'
(R):5'- GAATTCATTCTGGAGAGAAACCC -3'

Posted On

2015-07-21