Incidental Mutation 'R4464:Zfp429'
| ID |
330280 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp429
|
| Ensembl Gene |
ENSMUSG00000078994 |
| Gene Name |
zinc finger protein 429 |
| Synonyms |
2810487A22Rik |
| MMRRC Submission |
041722-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.068)
|
| Stock # |
R4464 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
13 |
| Chromosomal Location |
67536024-67547938 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to C
at 67538617 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Isoleucine to Valine
at position 276
(I276V)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000105354
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000109732]
[ENSMUST00000181071]
[ENSMUST00000224684]
[ENSMUST00000224825]
|
| AlphaFold |
Q7M6Y0 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000109732
AA Change: I276V
PolyPhen 2
Score 0.132 (Sensitivity: 0.93; Specificity: 0.86)
|
| SMART Domains |
Protein: ENSMUSP00000105354
Gene: ENSMUSG00000078994
AA Change: I276V
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
15 |
75 |
7.16e-34 |
SMART |
|
ZnF_C2H2
|
119 |
141 |
5.12e1 |
SMART |
|
ZnF_C2H2
|
147 |
169 |
2.27e-4 |
SMART |
|
ZnF_C2H2
|
175 |
197 |
1.28e-3 |
SMART |
|
ZnF_C2H2
|
203 |
225 |
1.56e-2 |
SMART |
|
ZnF_C2H2
|
259 |
281 |
4.62e1 |
SMART |
|
ZnF_C2H2
|
287 |
309 |
5.14e-3 |
SMART |
|
ZnF_C2H2
|
315 |
337 |
6.78e-3 |
SMART |
|
ZnF_C2H2
|
343 |
365 |
3.11e-2 |
SMART |
|
ZnF_C2H2
|
371 |
393 |
1.25e-1 |
SMART |
|
ZnF_C2H2
|
399 |
421 |
6.32e-3 |
SMART |
|
ZnF_C2H2
|
427 |
449 |
1.47e-3 |
SMART |
|
ZnF_C2H2
|
455 |
477 |
5.42e-2 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000181071
|
| SMART Domains |
Protein: ENSMUSP00000137755
Gene: ENSMUSG00000078994
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
15 |
75 |
7.16e-34 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000224684
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000224825
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000225810
|
| Meta Mutation Damage Score |
0.0898 |
| Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.5%
- 10x: 97.1%
- 20x: 94.8%
|
| Validation Efficiency |
97% (35/36) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 31 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 0610040J01Rik |
G |
T |
5: 64,056,182 (GRCm39) |
|
probably benign |
Het |
| Abcc3 |
A |
G |
11: 94,249,612 (GRCm39) |
V1111A |
probably benign |
Het |
| Acot10 |
G |
A |
15: 20,665,830 (GRCm39) |
R304* |
probably null |
Het |
| Aldh8a1 |
C |
A |
10: 21,264,840 (GRCm39) |
|
probably benign |
Het |
| Alms1 |
A |
G |
6: 85,597,003 (GRCm39) |
T1079A |
possibly damaging |
Het |
| Armc3 |
T |
C |
2: 19,253,470 (GRCm39) |
Y204H |
probably damaging |
Het |
| Asnsd1 |
C |
A |
1: 53,391,686 (GRCm39) |
|
probably null |
Het |
| Atad5 |
T |
A |
11: 79,991,137 (GRCm39) |
|
probably null |
Het |
| Cst12 |
G |
A |
2: 148,631,437 (GRCm39) |
V53I |
possibly damaging |
Het |
| Cylc2 |
C |
G |
4: 51,229,651 (GRCm39) |
T331R |
unknown |
Het |
| Dennd1a |
A |
T |
2: 38,133,402 (GRCm39) |
|
probably benign |
Het |
| Gm7535 |
C |
A |
17: 18,131,924 (GRCm39) |
|
probably benign |
Het |
| Gpr158 |
T |
A |
2: 21,831,810 (GRCm39) |
M970K |
probably damaging |
Het |
| Ifngr1 |
G |
A |
10: 19,473,265 (GRCm39) |
V72I |
possibly damaging |
Het |
| Kifap3 |
C |
A |
1: 163,645,464 (GRCm39) |
Q269K |
probably benign |
Het |
| Krt86 |
G |
A |
15: 101,371,795 (GRCm39) |
D122N |
probably damaging |
Het |
| Lrrcc1 |
A |
G |
3: 14,622,378 (GRCm39) |
K694E |
probably damaging |
Het |
| Mbd4 |
A |
G |
6: 115,826,463 (GRCm39) |
L155S |
probably damaging |
Het |
| Nalcn |
T |
C |
14: 123,560,762 (GRCm39) |
N772D |
probably benign |
Het |
| Or12d17 |
C |
T |
17: 37,777,742 (GRCm39) |
S215F |
probably damaging |
Het |
| Prxl2a |
T |
A |
14: 40,719,832 (GRCm39) |
K127N |
probably damaging |
Het |
| Psg29 |
A |
T |
7: 16,944,575 (GRCm39) |
N362Y |
possibly damaging |
Het |
| Ptpn23 |
G |
A |
9: 110,215,881 (GRCm39) |
T1325I |
probably damaging |
Het |
| Rad51ap1 |
T |
C |
6: 126,911,731 (GRCm39) |
N52S |
possibly damaging |
Het |
| Rb1 |
C |
A |
14: 73,436,638 (GRCm39) |
|
probably null |
Het |
| Slc34a2 |
T |
C |
5: 53,226,524 (GRCm39) |
L490P |
probably damaging |
Het |
| Sost |
G |
A |
11: 101,857,670 (GRCm39) |
P44S |
probably damaging |
Het |
| St3gal2 |
A |
G |
8: 111,694,134 (GRCm39) |
N207D |
probably benign |
Het |
| Stat1 |
T |
G |
1: 52,176,575 (GRCm39) |
D257E |
possibly damaging |
Het |
| Tkt |
A |
G |
14: 30,290,231 (GRCm39) |
T165A |
possibly damaging |
Het |
| Trim66 |
A |
T |
7: 109,076,897 (GRCm39) |
S347R |
possibly damaging |
Het |
|
| Other mutations in Zfp429 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01160:Zfp429
|
APN |
13 |
67,539,132 (GRCm39) |
missense |
probably damaging |
0.96 |
|
IGL01913:Zfp429
|
APN |
13 |
67,544,793 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02343:Zfp429
|
APN |
13 |
67,538,844 (GRCm39) |
missense |
probably damaging |
0.98 |
|
IGL02679:Zfp429
|
APN |
13 |
67,547,855 (GRCm39) |
intron |
probably benign |
|
|
IGL03396:Zfp429
|
APN |
13 |
67,544,159 (GRCm39) |
splice site |
probably benign |
|
|
FR4342:Zfp429
|
UTSW |
13 |
67,544,769 (GRCm39) |
missense |
probably benign |
0.02 |
|
R0012:Zfp429
|
UTSW |
13 |
67,538,796 (GRCm39) |
missense |
probably benign |
0.01 |
|
R1232:Zfp429
|
UTSW |
13 |
67,538,751 (GRCm39) |
missense |
possibly damaging |
0.47 |
|
R1330:Zfp429
|
UTSW |
13 |
67,544,262 (GRCm39) |
splice site |
probably null |
|
|
R1653:Zfp429
|
UTSW |
13 |
67,538,043 (GRCm39) |
missense |
possibly damaging |
0.87 |
|
R1761:Zfp429
|
UTSW |
13 |
67,544,195 (GRCm39) |
missense |
probably benign |
0.28 |
|
R1813:Zfp429
|
UTSW |
13 |
67,538,505 (GRCm39) |
missense |
possibly damaging |
0.55 |
|
R2356:Zfp429
|
UTSW |
13 |
67,538,746 (GRCm39) |
missense |
probably benign |
|
|
R4280:Zfp429
|
UTSW |
13 |
67,538,914 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4283:Zfp429
|
UTSW |
13 |
67,538,914 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4789:Zfp429
|
UTSW |
13 |
67,538,523 (GRCm39) |
missense |
probably benign |
0.06 |
|
R5187:Zfp429
|
UTSW |
13 |
67,538,959 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R5250:Zfp429
|
UTSW |
13 |
67,538,638 (GRCm39) |
missense |
probably benign |
0.00 |
|
R6688:Zfp429
|
UTSW |
13 |
67,544,249 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R6772:Zfp429
|
UTSW |
13 |
67,538,317 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6989:Zfp429
|
UTSW |
13 |
67,538,080 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7041:Zfp429
|
UTSW |
13 |
67,538,830 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7101:Zfp429
|
UTSW |
13 |
67,538,931 (GRCm39) |
missense |
possibly damaging |
0.88 |
|
R7593:Zfp429
|
UTSW |
13 |
67,538,410 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7792:Zfp429
|
UTSW |
13 |
67,538,558 (GRCm39) |
nonsense |
probably null |
|
|
R8500:Zfp429
|
UTSW |
13 |
67,538,828 (GRCm39) |
nonsense |
probably null |
|
|
R8721:Zfp429
|
UTSW |
13 |
67,538,331 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R8891:Zfp429
|
UTSW |
13 |
67,538,830 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9364:Zfp429
|
UTSW |
13 |
67,538,531 (GRCm39) |
missense |
probably benign |
0.12 |
|
R9554:Zfp429
|
UTSW |
13 |
67,538,531 (GRCm39) |
missense |
probably benign |
0.12 |
|
| Predicted Primers |
PCR Primer
(F):5'- TGTCTTTTAAGGCAGGAAGATGAGG -3'
(R):5'- GGCCTTCAGTACTCGCTCAT -3'
Sequencing Primer
(F):5'- GGAATAGCATTTGCCACAGTC -3'
(R):5'- GAATTCATTCTGGAGAGAAACCC -3'
|
| Posted On |
2015-07-21 |
Incidental Mutation