Incidental Mutation 'R4477:Zfp770'
| ID |
331303 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp770
|
| Ensembl Gene |
ENSMUSG00000040321 |
| Gene Name |
zinc finger protein 770 |
| Synonyms |
6430601A21Rik |
| MMRRC Submission |
041734-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.233)
|
| Stock # |
R4477 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
2 |
| Chromosomal Location |
114023937-114031945 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
G to A
at 114027365 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Leucine to Phenylalanine
at position 235
(L235F)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000052194
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000050668]
|
| AlphaFold |
Q8BIQ8 |
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000050668
AA Change: L235F
PolyPhen 2
Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
|
| SMART Domains |
Protein: ENSMUSP00000052194
Gene: ENSMUSG00000040321
AA Change: L235F
| Domain | Start | End | E-Value | Type |
|---|
|
ZnF_C2H2
|
31 |
53 |
1.18e-2 |
SMART |
|
ZnF_C2H2
|
59 |
81 |
4.11e-2 |
SMART |
|
ZnF_C2H2
|
85 |
107 |
3.58e-2 |
SMART |
|
ZnF_C2H2
|
164 |
186 |
2.09e-3 |
SMART |
|
ZnF_C2H2
|
192 |
214 |
3.58e-2 |
SMART |
|
ZnF_C2H2
|
220 |
242 |
3.29e-1 |
SMART |
|
ZnF_C2H2
|
298 |
318 |
1.93e2 |
SMART |
|
low complexity region
|
341 |
354 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
485 |
507 |
5.9e-3 |
SMART |
|
ZnF_C2H2
|
513 |
535 |
1.82e-3 |
SMART |
|
low complexity region
|
576 |
595 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
640 |
662 |
9.58e-3 |
SMART |
|
ZnF_C2H2
|
668 |
690 |
7.37e-4 |
SMART |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000123562
|
| Meta Mutation Damage Score |
0.1143 |
| Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.6%
- 10x: 97.2%
- 20x: 95.2%
|
| Validation Efficiency |
93% (39/42) |
| Allele List at MGI |
All alleles(4) : Targeted(3) Gene trapped(1)
|
| Other mutations in this stock |
Total: 36 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Abcg4 |
A |
G |
9: 44,186,383 (GRCm39) |
S549P |
probably damaging |
Het |
| Agfg1 |
T |
C |
1: 82,853,061 (GRCm39) |
S75P |
probably damaging |
Het |
| AK157302 |
T |
A |
13: 21,679,861 (GRCm39) |
V129E |
possibly damaging |
Het |
| Angpt1 |
A |
G |
15: 42,331,560 (GRCm39) |
Y344H |
probably damaging |
Het |
| Ap1m2 |
A |
G |
9: 21,209,509 (GRCm39) |
V389A |
probably benign |
Het |
| Bicd2 |
T |
A |
13: 49,531,448 (GRCm39) |
I230N |
probably damaging |
Het |
| C5ar1 |
T |
C |
7: 15,982,789 (GRCm39) |
N77S |
probably damaging |
Het |
| Cacna1c |
C |
T |
6: 118,607,200 (GRCm39) |
V1235M |
possibly damaging |
Het |
| Cdh15 |
A |
G |
8: 123,591,415 (GRCm39) |
H517R |
probably benign |
Het |
| D130040H23Rik |
C |
A |
8: 69,755,155 (GRCm39) |
H187N |
possibly damaging |
Het |
| Dbn1 |
CCCGCTCCCGGTAGCGCCGCTC |
CCCGCTC |
13: 55,629,374 (GRCm39) |
|
probably benign |
Het |
| Eif4g1 |
G |
T |
16: 20,497,593 (GRCm39) |
|
probably benign |
Het |
| Fmn1 |
T |
A |
2: 113,274,744 (GRCm39) |
|
probably benign |
Het |
| Gm3159 |
T |
C |
14: 4,398,584 (GRCm38) |
Y92H |
probably damaging |
Het |
| Gm7138 |
A |
T |
10: 77,612,246 (GRCm39) |
|
probably benign |
Het |
| Ift172 |
C |
T |
5: 31,422,781 (GRCm39) |
A890T |
probably benign |
Het |
| Inpp5j |
T |
C |
11: 3,451,625 (GRCm39) |
T426A |
probably damaging |
Het |
| Katna1 |
T |
C |
10: 7,614,594 (GRCm39) |
V32A |
probably damaging |
Het |
| Lrrc71 |
G |
C |
3: 87,649,972 (GRCm39) |
R319G |
probably damaging |
Het |
| Lyst |
G |
A |
13: 13,809,968 (GRCm39) |
R546H |
probably damaging |
Het |
| Mmp19 |
A |
T |
10: 128,631,506 (GRCm39) |
T129S |
probably benign |
Het |
| Mrc2 |
G |
A |
11: 105,239,257 (GRCm39) |
|
probably null |
Het |
| Neo1 |
T |
C |
9: 58,784,582 (GRCm39) |
D1458G |
probably damaging |
Het |
| Nup35 |
T |
C |
2: 80,487,487 (GRCm39) |
|
probably benign |
Het |
| Obscn |
T |
C |
11: 59,022,472 (GRCm39) |
R758G |
possibly damaging |
Het |
| Pdlim5 |
C |
T |
3: 141,964,978 (GRCm39) |
S417N |
probably benign |
Het |
| Pla2g4f |
A |
G |
2: 120,134,153 (GRCm39) |
S478P |
probably damaging |
Het |
| Plekhn1 |
G |
A |
4: 156,307,856 (GRCm39) |
R357W |
probably damaging |
Het |
| Pom121 |
T |
C |
5: 135,410,842 (GRCm39) |
T772A |
unknown |
Het |
| Pramel20 |
A |
G |
4: 143,297,732 (GRCm39) |
I51V |
probably benign |
Het |
| Rasgef1a |
A |
T |
6: 118,062,436 (GRCm39) |
H232L |
possibly damaging |
Het |
| Sdad1 |
A |
G |
5: 92,445,019 (GRCm39) |
M315T |
probably damaging |
Het |
| Syt9 |
A |
G |
7: 107,024,428 (GRCm39) |
N107S |
probably damaging |
Het |
| Traf3 |
T |
C |
12: 111,215,036 (GRCm39) |
S202P |
probably benign |
Het |
| Vmn2r9 |
T |
C |
5: 108,994,143 (GRCm39) |
E502G |
probably benign |
Het |
| Vps8 |
A |
T |
16: 21,363,986 (GRCm39) |
|
probably benign |
Het |
|
| Other mutations in Zfp770 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00090:Zfp770
|
APN |
2 |
114,026,413 (GRCm39) |
missense |
probably benign |
0.20 |
|
IGL00478:Zfp770
|
APN |
2 |
114,027,946 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01539:Zfp770
|
APN |
2 |
114,027,574 (GRCm39) |
missense |
probably damaging |
0.99 |
|
IGL01778:Zfp770
|
APN |
2 |
114,026,719 (GRCm39) |
missense |
probably damaging |
0.96 |
|
IGL02596:Zfp770
|
APN |
2 |
114,026,308 (GRCm39) |
missense |
probably benign |
0.11 |
|
IGL03227:Zfp770
|
APN |
2 |
114,027,570 (GRCm39) |
nonsense |
probably null |
|
|
R0057:Zfp770
|
UTSW |
2 |
114,027,713 (GRCm39) |
nonsense |
probably null |
|
|
R0057:Zfp770
|
UTSW |
2 |
114,027,713 (GRCm39) |
nonsense |
probably null |
|
|
R1081:Zfp770
|
UTSW |
2 |
114,027,608 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1446:Zfp770
|
UTSW |
2 |
114,027,514 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4597:Zfp770
|
UTSW |
2 |
114,027,251 (GRCm39) |
missense |
possibly damaging |
0.46 |
|
R4964:Zfp770
|
UTSW |
2 |
114,027,868 (GRCm39) |
missense |
probably benign |
0.24 |
|
R4966:Zfp770
|
UTSW |
2 |
114,027,868 (GRCm39) |
missense |
probably benign |
0.24 |
|
R5259:Zfp770
|
UTSW |
2 |
114,027,674 (GRCm39) |
missense |
probably benign |
0.00 |
|
R5440:Zfp770
|
UTSW |
2 |
114,026,596 (GRCm39) |
missense |
probably benign |
0.42 |
|
R5910:Zfp770
|
UTSW |
2 |
114,026,713 (GRCm39) |
nonsense |
probably null |
|
|
R5941:Zfp770
|
UTSW |
2 |
114,028,027 (GRCm39) |
missense |
possibly damaging |
0.83 |
|
R6074:Zfp770
|
UTSW |
2 |
114,026,870 (GRCm39) |
missense |
possibly damaging |
0.68 |
|
R6341:Zfp770
|
UTSW |
2 |
114,027,240 (GRCm39) |
missense |
probably benign |
0.14 |
|
R7181:Zfp770
|
UTSW |
2 |
114,027,872 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7288:Zfp770
|
UTSW |
2 |
114,026,142 (GRCm39) |
nonsense |
probably null |
|
|
R7935:Zfp770
|
UTSW |
2 |
114,027,305 (GRCm39) |
missense |
probably benign |
0.00 |
|
R8119:Zfp770
|
UTSW |
2 |
114,027,508 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8304:Zfp770
|
UTSW |
2 |
114,027,891 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9356:Zfp770
|
UTSW |
2 |
114,026,917 (GRCm39) |
missense |
possibly damaging |
0.83 |
|
R9657:Zfp770
|
UTSW |
2 |
114,027,766 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R9663:Zfp770
|
UTSW |
2 |
114,026,949 (GRCm39) |
missense |
probably benign |
0.01 |
|
| Predicted Primers |
PCR Primer
(F):5'- TCCGAACACTGAAAAGGGAC -3'
(R):5'- TCTGTGGCAAAATGTTTCCG -3'
Sequencing Primer
(F):5'- GGACAATGTAAATTGAGTGGACATC -3'
(R):5'- GTGGCAAAATGTTTCCGTCACAATC -3'
|
| Posted On |
2015-07-21 |
Incidental Mutation