Mutagenetix > Incidental Mutation

Incidental Mutation 'R4501:Pldi'

331818

Institutional Source

Beutler Lab

Gene Symbol

Pldi

Ensembl Gene

ENSMUSG00000037247

Gene Name

polymorphic derived intron containing

Synonyms

Poldi, 1700125F08Rik

MMRRC Submission

041753-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.122) question?

Stock #

R4501 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

60764005-60773911 bp(-) (GRCm39)

Type of Mutation

unclassified

DNA Base Change (assembly)

G to T at 60764188 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s):

AlphaFold

no structure available at present

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000036304

SMART Domains

Protein: ENSMUSP00000043979
Gene: ENSMUSG00000037247

Domain	Start	End	E-Value	Type
low complexity region	54	62	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000182497

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 95.0%

Validation Efficiency

100% (37/37)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit reduced testis weight and decreased sperm motility. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 31 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acin1	A	G	14: 54,924,044 (GRCm39)	V65A	probably damaging	Het
Ankrd28	A	G	14: 31,428,753 (GRCm39)	L956S	probably damaging	Het
Atp2a1	G	A	7: 126,052,555 (GRCm39)	T388I	probably benign	Het
AU018091	A	G	7: 3,208,919 (GRCm39)	V389A	probably benign	Het
Cdh2	A	G	18: 16,762,642 (GRCm39)	V434A	possibly damaging	Het
Dennd4a	G	A	9: 64,817,405 (GRCm39)	D1680N	possibly damaging	Het
Dnah2	T	A	11: 69,368,485 (GRCm39)	M1717L	probably benign	Het
Dusp6	T	A	10: 99,100,457 (GRCm39)	L151Q	probably benign	Het
Hc	A	T	2: 34,887,488 (GRCm39)		probably null	Het
Hmcn1	A	T	1: 150,509,417 (GRCm39)	S3644T	probably damaging	Het
Kcnt2	T	C	1: 140,480,718 (GRCm39)	I761T	probably damaging	Het
Mmd2	A	G	5: 142,560,965 (GRCm39)	V90A	probably benign	Het
Ncf2	C	G	1: 152,710,784 (GRCm39)	Q432E	probably benign	Het
Nxn	T	C	11: 76,165,438 (GRCm39)	E172G	probably damaging	Het
P2ry12	A	G	3: 59,125,078 (GRCm39)	I199T	probably damaging	Het
Phldb3	A	G	7: 24,311,986 (GRCm39)	E100G	probably benign	Het
Pidd1	A	G	7: 141,021,356 (GRCm39)		probably benign	Het
Plk5	T	C	10: 80,195,305 (GRCm39)	C208R	probably benign	Het
Ptpn12	G	T	5: 21,224,278 (GRCm39)	A105E	probably damaging	Het
Pusl1	T	C	4: 155,973,999 (GRCm39)	T252A	probably benign	Het
Rpl13a	T	C	7: 44,775,564 (GRCm39)	H95R	probably benign	Het
Sh3d21	GAATCTCCTGGGAAAATC	GAATC	4: 126,056,652 (GRCm39)		probably null	Het
Slc30a4	A	G	2: 122,527,136 (GRCm39)	I370T	probably benign	Het
Taf1c	A	G	8: 120,326,168 (GRCm39)	F565L	probably damaging	Het
Tdrd9	A	G	12: 112,009,243 (GRCm39)	K1050E	probably benign	Het
Tnrc6c	T	A	11: 117,613,324 (GRCm39)	L494Q	probably damaging	Het
Ttn	T	A	2: 76,624,991 (GRCm39)	I13450L	possibly damaging	Het
Usp34	C	A	11: 23,351,529 (GRCm39)	P1439Q	probably damaging	Het
Usp5	C	G	6: 124,799,593 (GRCm39)	K318N	possibly damaging	Het
Vmn1r80	A	G	7: 11,927,318 (GRCm39)	N143D	probably benign	Het
Zbtb44	G	A	9: 30,965,462 (GRCm39)	V291I	probably damaging	Het

Predicted Primers

PCR Primer
(F):5'- AGCCAATGAGGACAATCTGTC -3'
(R):5'- CAGGGCCAACTCATCTTTCCTG -3'

Sequencing Primer
(F):5'- AGGTTCTGACACTCAGAC -3'
(R):5'- CTGTTGTCTCTCAGCATAGTAATG -3'

Posted On

2015-07-21