Mutagenetix > Incidental Mutation

Incidental Mutation 'R4506:Gpr158'

332001

Institutional Source

Beutler Lab

Gene Symbol

Gpr158

Ensembl Gene

ENSMUSG00000045967

Gene Name

G protein-coupled receptor 158

Synonyms

5330427M13Rik

MMRRC Submission

041584-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4506 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

21367542-21830547 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 21826999 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 970 (M970K)

Ref Sequence

ENSEMBL: ENSMUSP00000049708 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055946]

AlphaFold

Q8C419

Predicted Effect

probably damaging
Transcript: ENSMUST00000055946
AA Change: M970K

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000049708
Gene: ENSMUSG00000045967
AA Change: M970K

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
low complexity region	110	125	N/A	INTRINSIC
SCOP:d1edmb_	313	359	5e-4	SMART
Blast:EGF	318	365	2e-27	BLAST
Pfam:7tm_3	426	669	1.2e-35	PFAM
low complexity region	840	863	N/A	INTRINSIC

Meta Mutation Damage Score

0.1418

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.1%
20x: 94.8%

Validation Efficiency

98% (42/43)

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700012B07Rik	G	T	11: 109,794,261 (GRCm38)	P137T	probably damaging	Het
Abcc5	A	T	16: 20,333,695 (GRCm38)	I1367N	probably damaging	Het
Ace	T	A	11: 105,976,666 (GRCm38)	L152Q	probably damaging	Het
Adam19	G	A	11: 46,118,444 (GRCm38)	D232N	possibly damaging	Het
Anapc4	A	T	5: 52,835,730 (GRCm38)	N61I	possibly damaging	Het
Ap5m1	T	C	14: 49,073,761 (GRCm38)	V96A	probably damaging	Het
Atr	T	A	9: 95,865,237 (GRCm38)	C191S	probably benign	Het
Carmil3	GGACGA	GGA	14: 55,499,476 (GRCm38)		probably benign	Het
Cnot6l	A	T	5: 96,086,174 (GRCm38)	V326E	possibly damaging	Het
Fcer2a	A	T	8: 3,688,603 (GRCm38)		probably null	Het
Fgfr3	A	G	5: 33,729,999 (GRCm38)	T221A	probably damaging	Het
Gm6632	C	T	5: 59,054,478 (GRCm38)		noncoding transcript	Het
Gm8909	A	T	17: 36,161,480 (GRCm38)		probably benign	Het
Ip6k3	A	T	17: 27,145,180 (GRCm38)	L298Q	possibly damaging	Het
Itpr1	A	G	6: 108,432,686 (GRCm38)	D1727G	probably damaging	Het
Kdm2b	T	C	5: 122,888,625 (GRCm38)	T589A	possibly damaging	Het
Krtcap2	A	G	3: 89,246,256 (GRCm38)		probably benign	Het
Map3k13	A	G	16: 21,922,178 (GRCm38)	S752G	probably benign	Het
Mvb12a	G	T	8: 71,543,459 (GRCm38)	A86S	probably benign	Het
Nktr	T	A	9: 121,748,883 (GRCm38)		probably benign	Het
Or1e33	A	T	11: 73,847,695 (GRCm38)	C143*	probably null	Het
Or8a1b	C	T	9: 37,711,464 (GRCm38)	V272I	probably benign	Het
Parp4	T	A	14: 56,652,304 (GRCm38)	N1847K	unknown	Het
Pcdhga8	G	C	18: 37,816,763 (GRCm38)	V411L	probably damaging	Het
Piwil4	T	C	9: 14,725,963 (GRCm38)	T352A	probably damaging	Het
Ric8a	A	G	7: 140,858,516 (GRCm38)	I223V	probably benign	Het
Slc25a51	C	T	4: 45,399,768 (GRCm38)	V141M	probably benign	Het
Slc7a5	A	T	8: 121,887,495 (GRCm38)		probably null	Het
Slc7a9	A	T	7: 35,453,420 (GRCm38)	T88S	probably damaging	Het
Tbrg1	T	A	9: 37,654,395 (GRCm38)	E87V	probably damaging	Het
Tnfsf4	T	C	1: 161,417,174 (GRCm38)	S145P	probably damaging	Het
Tomm34	C	A	2: 164,054,372 (GRCm38)		probably null	Het
Trpv3	A	G	11: 73,295,324 (GRCm38)	N647S	probably benign	Het
Upf1	G	A	8: 70,337,566 (GRCm38)	R637C	probably damaging	Het
Vmn2r23	A	T	6: 123,702,925 (GRCm38)	Q35H	probably damaging	Het
Wdtc1	C	T	4: 133,308,819 (GRCm38)	V137M	probably damaging	Het
Zfp276	G	A	8: 123,264,927 (GRCm38)		probably null	Het
Zfp90	C	T	8: 106,424,864 (GRCm38)	P403L	possibly damaging	Het

Other mutations in Gpr158

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00340:Gpr158	APN	2	21,368,683 (GRCm38)	missense	probably damaging	1.00
IGL00469:Gpr158	APN	2	21,746,795 (GRCm38)	splice site	probably benign
IGL00706:Gpr158	APN	2	21,746,773 (GRCm38)	missense	probably damaging	1.00
IGL00780:Gpr158	APN	2	21,826,818 (GRCm38)	nonsense	probably null
IGL00885:Gpr158	APN	2	21,649,021 (GRCm38)	missense	probably damaging	1.00
IGL01339:Gpr158	APN	2	21,369,031 (GRCm38)	missense	possibly damaging	0.73
IGL01368:Gpr158	APN	2	21,827,098 (GRCm38)	missense	probably damaging	1.00
IGL02141:Gpr158	APN	2	21,783,290 (GRCm38)	missense	probably damaging	0.99
IGL02455:Gpr158	APN	2	21,368,700 (GRCm38)	missense	probably benign	0.00
IGL02554:Gpr158	APN	2	21,826,596 (GRCm38)	missense	probably benign
IGL02681:Gpr158	APN	2	21,815,630 (GRCm38)	missense	probably damaging	1.00
IGL02752:Gpr158	APN	2	21,826,827 (GRCm38)	missense	possibly damaging	0.95
IGL02756:Gpr158	APN	2	21,827,079 (GRCm38)	missense	possibly damaging	0.47
IGL03181:Gpr158	APN	2	21,783,161 (GRCm38)	missense	probably benign	0.02
IGL03258:Gpr158	APN	2	21,825,274 (GRCm38)	missense	probably damaging	1.00
IGL03386:Gpr158	APN	2	21,826,246 (GRCm38)	missense	probably damaging	1.00
PIT4810001:Gpr158	UTSW	2	21,826,871 (GRCm38)	missense	probably benign	0.01
R0071:Gpr158	UTSW	2	21,810,668 (GRCm38)	missense	probably benign	0.08
R0081:Gpr158	UTSW	2	21,826,717 (GRCm38)	missense	probably damaging	1.00
R0528:Gpr158	UTSW	2	21,825,208 (GRCm38)	missense	probably damaging	1.00
R0560:Gpr158	UTSW	2	21,825,274 (GRCm38)	missense	probably damaging	1.00
R0603:Gpr158	UTSW	2	21,815,669 (GRCm38)	missense	possibly damaging	0.67
R1560:Gpr158	UTSW	2	21,826,314 (GRCm38)	missense	probably damaging	1.00
R1561:Gpr158	UTSW	2	21,815,694 (GRCm38)	splice site	probably null
R1609:Gpr158	UTSW	2	21,783,293 (GRCm38)	missense	possibly damaging	0.61
R1741:Gpr158	UTSW	2	21,827,548 (GRCm38)	missense	probably benign	0.00
R1827:Gpr158	UTSW	2	21,827,318 (GRCm38)	missense	probably benign
R1854:Gpr158	UTSW	2	21,369,124 (GRCm38)	missense	probably damaging	1.00
R1871:Gpr158	UTSW	2	21,815,615 (GRCm38)	missense	probably damaging	1.00
R2151:Gpr158	UTSW	2	21,827,514 (GRCm38)	missense	possibly damaging	0.82
R2273:Gpr158	UTSW	2	21,826,863 (GRCm38)	missense	probably benign
R2275:Gpr158	UTSW	2	21,826,863 (GRCm38)	missense	probably benign
R3004:Gpr158	UTSW	2	21,826,999 (GRCm38)	missense	probably damaging	0.99
R3151:Gpr158	UTSW	2	21,576,960 (GRCm38)	missense	possibly damaging	0.68
R3943:Gpr158	UTSW	2	21,368,559 (GRCm38)	missense	possibly damaging	0.65
R4238:Gpr158	UTSW	2	21,368,551 (GRCm38)	missense	probably damaging	1.00
R4379:Gpr158	UTSW	2	21,825,214 (GRCm38)	missense	probably damaging	1.00
R4381:Gpr158	UTSW	2	21,827,592 (GRCm38)	missense	probably damaging	1.00
R4464:Gpr158	UTSW	2	21,826,999 (GRCm38)	missense	probably damaging	0.99
R4467:Gpr158	UTSW	2	21,826,999 (GRCm38)	missense	probably damaging	0.99
R4496:Gpr158	UTSW	2	21,826,999 (GRCm38)	missense	probably damaging	0.99
R4530:Gpr158	UTSW	2	21,369,000 (GRCm38)	missense	probably benign	0.03
R4646:Gpr158	UTSW	2	21,827,053 (GRCm38)	missense	probably benign
R4798:Gpr158	UTSW	2	21,783,182 (GRCm38)	missense	probably damaging	1.00
R4882:Gpr158	UTSW	2	21,825,248 (GRCm38)	missense	probably damaging	0.98
R4943:Gpr158	UTSW	2	21,827,157 (GRCm38)	missense	probably damaging	1.00
R5334:Gpr158	UTSW	2	21,827,505 (GRCm38)	missense	probably benign	0.01
R5560:Gpr158	UTSW	2	21,826,290 (GRCm38)	missense	possibly damaging	0.67
R5600:Gpr158	UTSW	2	21,827,235 (GRCm38)	missense	probably benign
R5637:Gpr158	UTSW	2	21,783,272 (GRCm38)	missense	probably benign	0.00
R5701:Gpr158	UTSW	2	21,746,709 (GRCm38)	missense	probably damaging	1.00
R5744:Gpr158	UTSW	2	21,368,520 (GRCm38)	missense	probably damaging	1.00
R5911:Gpr158	UTSW	2	21,369,121 (GRCm38)	missense	possibly damaging	0.95
R5991:Gpr158	UTSW	2	21,368,508 (GRCm38)	missense	probably damaging	0.99
R6200:Gpr158	UTSW	2	21,399,416 (GRCm38)	missense	probably damaging	0.97
R6306:Gpr158	UTSW	2	21,815,611 (GRCm38)	missense	possibly damaging	0.84
R6324:Gpr158	UTSW	2	21,810,554 (GRCm38)	missense	probably damaging	1.00
R6384:Gpr158	UTSW	2	21,826,288 (GRCm38)	missense	probably damaging	1.00
R6698:Gpr158	UTSW	2	21,827,110 (GRCm38)	missense	probably damaging	1.00
R6997:Gpr158	UTSW	2	21,648,991 (GRCm38)	missense	possibly damaging	0.46
R7086:Gpr158	UTSW	2	21,826,575 (GRCm38)	missense	probably benign	0.01
R7175:Gpr158	UTSW	2	21,368,302 (GRCm38)	missense	probably benign	0.13
R7197:Gpr158	UTSW	2	21,810,601 (GRCm38)	missense	probably damaging	0.99
R7293:Gpr158	UTSW	2	21,576,939 (GRCm38)	missense	possibly damaging	0.47
R7427:Gpr158	UTSW	2	21,827,318 (GRCm38)	missense	probably benign
R7515:Gpr158	UTSW	2	21,368,281 (GRCm38)	missense	probably damaging	1.00
R7730:Gpr158	UTSW	2	21,826,347 (GRCm38)	missense	probably damaging	1.00
R8122:Gpr158	UTSW	2	21,826,863 (GRCm38)	missense	probably benign
R8311:Gpr158	UTSW	2	21,368,890 (GRCm38)	missense	probably benign	0.00
R8754:Gpr158	UTSW	2	21,576,882 (GRCm38)	missense	probably benign	0.00
R8782:Gpr158	UTSW	2	21,399,338 (GRCm38)	missense	probably damaging	1.00
R8792:Gpr158	UTSW	2	21,553,326 (GRCm38)	missense	probably damaging	1.00
R8842:Gpr158	UTSW	2	21,576,940 (GRCm38)	missense	possibly damaging	0.88
R9009:Gpr158	UTSW	2	21,576,949 (GRCm38)	missense	probably damaging	1.00
R9102:Gpr158	UTSW	2	21,825,267 (GRCm38)	missense	probably damaging	1.00
R9150:Gpr158	UTSW	2	21,826,440 (GRCm38)	missense	probably benign	0.17
R9254:Gpr158	UTSW	2	21,368,231 (GRCm38)	start gained	probably benign
R9317:Gpr158	UTSW	2	21,827,226 (GRCm38)	missense	probably benign
R9379:Gpr158	UTSW	2	21,368,231 (GRCm38)	start gained	probably benign
R9428:Gpr158	UTSW	2	21,783,161 (GRCm38)	missense	probably benign
R9497:Gpr158	UTSW	2	21,827,014 (GRCm38)	missense	probably benign	0.00
R9667:Gpr158	UTSW	2	21,825,243 (GRCm38)	missense	probably damaging	0.99
R9681:Gpr158	UTSW	2	21,826,504 (GRCm38)	missense	probably damaging	0.99
X0062:Gpr158	UTSW	2	21,826,369 (GRCm38)	missense	probably damaging	1.00
Z1176:Gpr158	UTSW	2	21,810,690 (GRCm38)	critical splice donor site	probably null
Z1177:Gpr158	UTSW	2	21,827,272 (GRCm38)	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- GTCATTGCAAGTGCCAAGGAG -3'
(R):5'- AAAACGAAGCAGGGTTTTGTTCC -3'

Sequencing Primer
(F):5'- AGAAGACACTTGGCCTGGCTG -3'
(R):5'- CACTTCAGAAGCTGCGATTG -3'

Posted On

2015-07-21