Mutagenetix > Incidental Mutation

Incidental Mutation 'R0105:Spen'

33248

Institutional Source

Beutler Lab

Gene Symbol

Spen

Ensembl Gene

ENSMUSG00000040761

Gene Name

spen family transcription repressor

Synonyms

Mint

MMRRC Submission

038391-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R0105 (G1)

Quality Score

143

Status

Validated (trace)

Chromosome

Chromosomal Location

141195201-141265908 bp(-) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

T to C at 141197121 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000101412 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000006377] [ENSMUST00000078886] [ENSMUST00000105786]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000006377

SMART Domains

Protein: ENSMUSP00000006377
Gene: ENSMUSG00000006215

Domain	Start	End	E-Value	Type
BTB	24	116	1.38e-27	SMART
low complexity region	203	222	N/A	INTRINSIC
ZnF_C2H2	297	319	6.42e-4	SMART
ZnF_C2H2	325	347	3.11e-2	SMART
ZnF_C2H2	353	375	2.49e-1	SMART
ZnF_C2H2	381	403	8.47e-4	SMART
ZnF_C2H2	409	431	8.47e-4	SMART
ZnF_C2H2	437	459	1.22e-4	SMART
ZnF_C2H2	465	487	4.94e-5	SMART
ZnF_C2H2	493	515	3.26e-5	SMART
ZnF_C2H2	521	543	7.26e-3	SMART
ZnF_C2H2	549	571	4.79e-3	SMART
ZnF_C2H2	577	599	1.58e-3	SMART
ZnF_C2H2	605	628	2.57e-3	SMART
low complexity region	654	674	N/A	INTRINSIC
ZnF_C2H2	708	730	4.4e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000078886

SMART Domains

Protein: ENSMUSP00000077925
Gene: ENSMUSG00000040761

Domain	Start	End	E-Value	Type
RRM	7	77	7.77e-12	SMART
low complexity region	109	121	N/A	INTRINSIC
low complexity region	235	257	N/A	INTRINSIC
low complexity region	262	311	N/A	INTRINSIC
RRM	338	411	8.6e-5	SMART
RRM	441	511	1.56e-16	SMART
RRM	520	587	1.84e-13	SMART
low complexity region	617	632	N/A	INTRINSIC
low complexity region	669	691	N/A	INTRINSIC
low complexity region	695	720	N/A	INTRINSIC
low complexity region	749	773	N/A	INTRINSIC
coiled coil region	800	825	N/A	INTRINSIC
low complexity region	830	841	N/A	INTRINSIC
internal_repeat_2	844	954	6.27e-5	PROSPERO
coiled coil region	1494	1522	N/A	INTRINSIC
low complexity region	1587	1627	N/A	INTRINSIC
low complexity region	1635	1641	N/A	INTRINSIC
low complexity region	1642	1671	N/A	INTRINSIC
low complexity region	1747	1758	N/A	INTRINSIC
low complexity region	1810	1823	N/A	INTRINSIC
low complexity region	1888	1903	N/A	INTRINSIC
low complexity region	1940	1955	N/A	INTRINSIC
low complexity region	2003	2012	N/A	INTRINSIC
internal_repeat_2	2015	2115	6.27e-5	PROSPERO
low complexity region	2127	2147	N/A	INTRINSIC
low complexity region	2169	2191	N/A	INTRINSIC
low complexity region	2207	2219	N/A	INTRINSIC
low complexity region	2304	2323	N/A	INTRINSIC
low complexity region	2332	2371	N/A	INTRINSIC
low complexity region	2396	2413	N/A	INTRINSIC
low complexity region	2518	2533	N/A	INTRINSIC
low complexity region	2545	2555	N/A	INTRINSIC
low complexity region	2696	2722	N/A	INTRINSIC
low complexity region	2931	2942	N/A	INTRINSIC
low complexity region	2994	3006	N/A	INTRINSIC
low complexity region	3192	3212	N/A	INTRINSIC
low complexity region	3299	3337	N/A	INTRINSIC
Pfam:SPOC	3465	3586	2.7e-29	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000105786

SMART Domains

Protein: ENSMUSP00000101412
Gene: ENSMUSG00000040761

Domain	Start	End	E-Value	Type
RRM	7	77	7.77e-12	SMART
low complexity region	109	121	N/A	INTRINSIC
low complexity region	235	257	N/A	INTRINSIC
low complexity region	262	311	N/A	INTRINSIC
RRM	338	411	8.6e-5	SMART
RRM	441	511	1.56e-16	SMART
RRM	520	587	1.84e-13	SMART
low complexity region	692	714	N/A	INTRINSIC
low complexity region	718	743	N/A	INTRINSIC
low complexity region	772	796	N/A	INTRINSIC
coiled coil region	823	848	N/A	INTRINSIC
low complexity region	853	864	N/A	INTRINSIC
internal_repeat_2	867	977	8.58e-5	PROSPERO
coiled coil region	1517	1545	N/A	INTRINSIC
low complexity region	1610	1650	N/A	INTRINSIC
low complexity region	1658	1664	N/A	INTRINSIC
low complexity region	1665	1694	N/A	INTRINSIC
low complexity region	1770	1781	N/A	INTRINSIC
low complexity region	1833	1846	N/A	INTRINSIC
low complexity region	1911	1926	N/A	INTRINSIC
low complexity region	1963	1978	N/A	INTRINSIC
low complexity region	2026	2035	N/A	INTRINSIC
internal_repeat_2	2038	2138	8.58e-5	PROSPERO
low complexity region	2150	2170	N/A	INTRINSIC
low complexity region	2192	2214	N/A	INTRINSIC
low complexity region	2230	2242	N/A	INTRINSIC
low complexity region	2327	2346	N/A	INTRINSIC
low complexity region	2355	2394	N/A	INTRINSIC
low complexity region	2419	2436	N/A	INTRINSIC
low complexity region	2541	2556	N/A	INTRINSIC
low complexity region	2568	2578	N/A	INTRINSIC
low complexity region	2719	2745	N/A	INTRINSIC
low complexity region	2954	2965	N/A	INTRINSIC
low complexity region	3017	3029	N/A	INTRINSIC
low complexity region	3215	3235	N/A	INTRINSIC
low complexity region	3322	3360	N/A	INTRINSIC
Pfam:SPOC	3488	3609	2.7e-29	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000123477

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000130482

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000142020

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000142438

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000142695

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000147227

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.0%
20x: 94.9%

Validation Efficiency

100% (70/70)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a hormone inducible transcriptional repressor. Repression of transcription by this gene product can occur through interactions with other repressors, by the recruitment of proteins involved in histone deacetylation, or through sequestration of transcriptional activators. The product of this gene contains a carboxy-terminal domain that permits binding to other corepressor proteins. This domain also permits interaction with members of the NuRD complex, a nucleosome remodeling protein complex that contains deacetylase activity. In addition, this repressor contains several RNA recognition motifs that confer binding to a steroid receptor RNA coactivator; this binding can modulate the activity of both liganded and nonliganded steroid receptors. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous mutant mice die during late gestation exhibiting morphological abnormalities of the heart, pancreas, and liver. Inactivation of this gene also affects the differentiation of B cells. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5530400C23Rik	G	A	6: 133,271,277 (GRCm39)	R107K	probably benign	Het
A530053G22Rik	T	C	6: 60,379,137 (GRCm39)		noncoding transcript	Het
Adcy9	A	G	16: 4,106,252 (GRCm39)	V954A	probably damaging	Het
Aldh8a1	T	A	10: 21,271,438 (GRCm39)	M388K	probably damaging	Het
Ankhd1	A	G	18: 36,779,819 (GRCm39)	I1720M	probably damaging	Het
Atp6v0a4	T	C	6: 38,030,064 (GRCm39)		probably benign	Het
C1qtnf4	T	A	2: 90,720,707 (GRCm39)	*327R	probably null	Het
C1s1	T	C	6: 124,518,277 (GRCm39)		probably benign	Het
Cdsn	A	C	17: 35,867,035 (GRCm39)	R521S	possibly damaging	Het
Cgnl1	T	C	9: 71,563,384 (GRCm39)	M848V	probably benign	Het
Cog3	A	G	14: 75,959,580 (GRCm39)	S591P	probably damaging	Het
Col6a3	A	G	1: 90,725,883 (GRCm39)	V1375A	possibly damaging	Het
Cplane1	T	A	15: 8,216,876 (GRCm39)	V698D	probably benign	Het
Cr1l	A	G	1: 194,794,720 (GRCm39)		probably benign	Het
Crmp1	T	A	5: 37,441,479 (GRCm39)	D520E	probably damaging	Het
Ctdspl2	T	A	2: 121,807,801 (GRCm39)		probably benign	Het
Dnah6	C	T	6: 73,132,262 (GRCm39)	A1147T	probably damaging	Het
Dsg2	T	C	18: 20,735,111 (GRCm39)	S1030P	probably benign	Het
Elavl3	C	A	9: 21,948,129 (GRCm39)	V12F	possibly damaging	Het
Fam20b	T	C	1: 156,518,140 (GRCm39)	E218G	probably damaging	Het
Fam227a	T	C	15: 79,505,033 (GRCm39)	D466G	possibly damaging	Het
Fto	G	A	8: 92,249,430 (GRCm39)	E421K	probably damaging	Het
Gab2	T	C	7: 96,948,279 (GRCm39)	Y290H	probably damaging	Het
Gm973	A	G	1: 59,621,633 (GRCm39)	Q591R	probably null	Het
Gsdmc2	T	C	15: 63,700,026 (GRCm39)	T249A	probably benign	Het
Il15ra	T	A	2: 11,735,459 (GRCm39)		probably null	Het
Il1rl1	CTTGTTGTTGTTGTTGTTG	CTTGTTGTTGTTGTTGTTGTTG	1: 40,481,734 (GRCm39)		probably benign	Het
Il6ra	A	G	3: 89,784,125 (GRCm39)	I382T	probably damaging	Het
Isy1	G	A	6: 87,796,167 (GRCm39)	R257W	probably damaging	Het
Krt76	T	C	15: 101,793,347 (GRCm39)	T564A	unknown	Het
Lhpp	T	C	7: 132,232,254 (GRCm39)	S57P	probably damaging	Het
Lrrk1	G	T	7: 65,942,089 (GRCm39)	D716E	probably damaging	Het
Mcm3ap	T	A	10: 76,335,368 (GRCm39)	D1263E	probably damaging	Het
Mogat1	A	G	1: 78,500,307 (GRCm39)	T124A	probably benign	Het
Mroh7	T	C	4: 106,568,467 (GRCm39)	T48A	possibly damaging	Het
Nccrp1	T	C	7: 28,246,463 (GRCm39)	D33G	probably benign	Het
Neurog1	G	T	13: 56,399,050 (GRCm39)	D232E	probably benign	Het
Or4a71	T	C	2: 89,358,707 (GRCm39)	T16A	probably benign	Het
Or4c105	T	A	2: 88,648,253 (GRCm39)	V246D	probably damaging	Het
Otog	C	A	7: 45,937,790 (GRCm39)	T1833K	possibly damaging	Het
Perm1	C	A	4: 156,302,682 (GRCm39)	H409N	probably benign	Het
Pik3r5	A	T	11: 68,381,337 (GRCm39)	E174D	probably damaging	Het
Pkhd1	G	A	1: 20,593,956 (GRCm39)	Q1386*	probably null	Het
Pla2r1	T	C	2: 60,345,325 (GRCm39)	R344G	possibly damaging	Het
Plekha5	G	A	6: 140,537,473 (GRCm39)	R646K	possibly damaging	Het
Plekhg4	G	A	8: 106,108,644 (GRCm39)	V1202M	possibly damaging	Het
Ppil4	A	G	10: 7,674,210 (GRCm39)	Y118C	probably damaging	Het
Prrc2b	G	T	2: 32,103,323 (GRCm39)	E934*	probably null	Het
Psmb9	A	G	17: 34,406,249 (GRCm39)	F12S	probably benign	Het
Ptdss2	T	C	7: 140,732,793 (GRCm39)	W183R	probably damaging	Het
Ptpn4	C	T	1: 119,615,335 (GRCm39)		probably null	Het
Reln	G	A	5: 22,253,813 (GRCm39)	R600W	probably damaging	Het
Scml4	T	A	10: 42,806,595 (GRCm39)	V161E	probably damaging	Het
Sdcbp2	A	T	2: 151,431,478 (GRCm39)	T284S	probably benign	Het
Slc22a29	T	C	19: 8,137,991 (GRCm39)		probably benign	Het
Slc35e1	T	C	8: 73,246,415 (GRCm39)		probably benign	Het
Sumf2	T	A	5: 129,878,735 (GRCm39)		probably benign	Het
Tbx10	A	G	19: 4,043,121 (GRCm39)		probably benign	Het
Tex10	C	A	4: 48,468,957 (GRCm39)	V73F	probably damaging	Het
Tgm5	C	A	2: 120,907,493 (GRCm39)	G77W	probably damaging	Het
Tnfrsf21	T	A	17: 43,351,082 (GRCm39)		probably null	Het
Treml2	C	T	17: 48,609,856 (GRCm39)	T96I	probably damaging	Het
Trim65	T	C	11: 116,016,892 (GRCm39)	*523W	probably null	Het
Zcchc17	T	A	4: 130,243,099 (GRCm39)	D28V	probably benign	Het
Zhx2	T	C	15: 57,686,091 (GRCm39)	F487L	probably damaging	Het
Zkscan6	T	A	11: 65,712,811 (GRCm39)	L248Q	probably damaging	Het

Other mutations in Spen

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01133:Spen	APN	4	141,217,212 (GRCm39)	missense	unknown
IGL01357:Spen	APN	4	141,244,424 (GRCm39)	missense	unknown
IGL02184:Spen	APN	4	141,214,917 (GRCm39)	missense	unknown
IGL02226:Spen	APN	4	141,205,457 (GRCm39)	missense	unknown
IGL02321:Spen	APN	4	141,244,441 (GRCm39)	missense	unknown
IGL02350:Spen	APN	4	141,204,890 (GRCm39)	missense	unknown
IGL02357:Spen	APN	4	141,204,890 (GRCm39)	missense	unknown
IGL02627:Spen	APN	4	141,200,326 (GRCm39)	missense	probably damaging	0.99
IGL02683:Spen	APN	4	141,198,956 (GRCm39)	missense	probably benign	0.06
IGL02945:Spen	APN	4	141,221,624 (GRCm39)	missense	unknown
IGL02950:Spen	APN	4	141,196,819 (GRCm39)	missense	probably damaging	1.00
IGL03008:Spen	APN	4	141,203,448 (GRCm39)	missense	possibly damaging	0.70
IGL03019:Spen	APN	4	141,206,227 (GRCm39)	missense	unknown
IGL03038:Spen	APN	4	141,265,550 (GRCm39)	missense	unknown
IGL03334:Spen	APN	4	141,197,280 (GRCm39)	missense	probably damaging	1.00
filtered	UTSW	4	141,204,683 (GRCm39)	missense	unknown
mentholated	UTSW	4	141,196,711 (GRCm39)	missense	possibly damaging	0.78
R0268:Spen	UTSW	4	141,204,868 (GRCm39)	missense	unknown
R0359:Spen	UTSW	4	141,244,181 (GRCm39)	missense	unknown
R0394:Spen	UTSW	4	141,201,514 (GRCm39)	missense	probably benign	0.03
R0423:Spen	UTSW	4	141,206,647 (GRCm39)	missense	unknown
R0433:Spen	UTSW	4	141,211,069 (GRCm39)	missense	unknown
R0462:Spen	UTSW	4	141,200,962 (GRCm39)	missense	probably damaging	1.00
R0687:Spen	UTSW	4	141,215,339 (GRCm39)	missense	unknown
R0699:Spen	UTSW	4	141,201,702 (GRCm39)	missense	possibly damaging	0.72
R0865:Spen	UTSW	4	141,199,181 (GRCm39)	missense	probably benign	0.11
R0918:Spen	UTSW	4	141,212,875 (GRCm39)	missense	unknown
R1034:Spen	UTSW	4	141,203,063 (GRCm39)	missense	probably benign	0.33
R1341:Spen	UTSW	4	141,196,711 (GRCm39)	missense	possibly damaging	0.78
R1401:Spen	UTSW	4	141,199,132 (GRCm39)	missense	probably damaging	0.98
R1509:Spen	UTSW	4	141,203,011 (GRCm39)	missense	possibly damaging	0.53
R1509:Spen	UTSW	4	141,202,946 (GRCm39)	missense	probably benign	0.00
R1561:Spen	UTSW	4	141,199,694 (GRCm39)	nonsense	probably null
R1589:Spen	UTSW	4	141,215,335 (GRCm39)	missense	unknown
R1640:Spen	UTSW	4	141,196,254 (GRCm39)	missense	probably damaging	0.98
R1758:Spen	UTSW	4	141,203,686 (GRCm39)	missense	unknown
R1764:Spen	UTSW	4	141,200,261 (GRCm39)	missense	probably damaging	1.00
R1824:Spen	UTSW	4	141,200,096 (GRCm39)	missense	probably damaging	1.00
R1899:Spen	UTSW	4	141,197,654 (GRCm39)	missense	probably benign	0.17
R1916:Spen	UTSW	4	141,199,909 (GRCm39)	missense	probably damaging	1.00
R2011:Spen	UTSW	4	141,200,640 (GRCm39)	missense	probably damaging	1.00
R2295:Spen	UTSW	4	141,204,584 (GRCm39)	missense	unknown
R2379:Spen	UTSW	4	141,244,238 (GRCm39)	missense	unknown
R2404:Spen	UTSW	4	141,205,216 (GRCm39)	missense	unknown
R3719:Spen	UTSW	4	141,244,494 (GRCm39)	missense	unknown
R3889:Spen	UTSW	4	141,205,192 (GRCm39)	missense	unknown
R3945:Spen	UTSW	4	141,204,664 (GRCm39)	missense	unknown
R4227:Spen	UTSW	4	141,249,458 (GRCm39)	missense	unknown
R4326:Spen	UTSW	4	141,204,683 (GRCm39)	missense	unknown
R4382:Spen	UTSW	4	141,200,450 (GRCm39)	missense	possibly damaging	0.88
R4542:Spen	UTSW	4	141,204,097 (GRCm39)	missense	unknown
R4757:Spen	UTSW	4	141,200,390 (GRCm39)	nonsense	probably null
R4771:Spen	UTSW	4	141,199,907 (GRCm39)	missense	probably benign	0.14
R5072:Spen	UTSW	4	141,249,613 (GRCm39)	missense	unknown
R5121:Spen	UTSW	4	141,203,410 (GRCm39)	missense	probably benign	0.00
R5176:Spen	UTSW	4	141,203,587 (GRCm39)	missense	unknown
R5290:Spen	UTSW	4	141,201,127 (GRCm39)	missense	probably damaging	1.00
R5291:Spen	UTSW	4	141,215,390 (GRCm39)	missense	unknown
R5293:Spen	UTSW	4	141,199,717 (GRCm39)	missense	possibly damaging	0.89
R5347:Spen	UTSW	4	141,198,796 (GRCm39)	missense	probably benign	0.26
R5511:Spen	UTSW	4	141,244,149 (GRCm39)	missense	unknown
R5511:Spen	UTSW	4	141,202,375 (GRCm39)	missense	possibly damaging	0.86
R5772:Spen	UTSW	4	141,205,495 (GRCm39)	missense	unknown
R5834:Spen	UTSW	4	141,199,154 (GRCm39)	missense	possibly damaging	0.63
R5858:Spen	UTSW	4	141,201,182 (GRCm39)	missense	probably benign	0.05
R6214:Spen	UTSW	4	141,206,423 (GRCm39)	missense	unknown
R6232:Spen	UTSW	4	141,244,333 (GRCm39)	missense	unknown
R6345:Spen	UTSW	4	141,198,944 (GRCm39)	missense	possibly damaging	0.86
R6419:Spen	UTSW	4	141,203,621 (GRCm39)	missense	unknown
R6455:Spen	UTSW	4	141,202,820 (GRCm39)	missense	probably damaging	0.97
R6979:Spen	UTSW	4	141,205,374 (GRCm39)	missense	unknown
R6994:Spen	UTSW	4	141,220,770 (GRCm39)	missense	unknown
R7018:Spen	UTSW	4	141,220,755 (GRCm39)	missense	unknown
R7040:Spen	UTSW	4	141,221,693 (GRCm39)	missense	unknown
R7127:Spen	UTSW	4	141,203,419 (GRCm39)	missense	possibly damaging	0.53
R7218:Spen	UTSW	4	141,199,961 (GRCm39)	missense	possibly damaging	0.54
R7234:Spen	UTSW	4	141,206,446 (GRCm39)	missense	unknown
R7316:Spen	UTSW	4	141,204,365 (GRCm39)	missense	unknown
R7350:Spen	UTSW	4	141,206,696 (GRCm39)	missense	unknown
R7356:Spen	UTSW	4	141,199,235 (GRCm39)	nonsense	probably null
R7400:Spen	UTSW	4	141,201,052 (GRCm39)	missense	probably damaging	1.00
R7470:Spen	UTSW	4	141,206,605 (GRCm39)	missense	unknown
R7698:Spen	UTSW	4	141,200,156 (GRCm39)	missense	probably damaging	1.00
R7858:Spen	UTSW	4	141,215,442 (GRCm39)	splice site	probably null
R8033:Spen	UTSW	4	141,199,057 (GRCm39)	missense	probably benign	0.03
R8064:Spen	UTSW	4	141,203,011 (GRCm39)	missense	possibly damaging	0.53
R8159:Spen	UTSW	4	141,202,314 (GRCm39)	missense	possibly damaging	0.53
R8187:Spen	UTSW	4	141,200,216 (GRCm39)	missense	possibly damaging	0.93
R8463:Spen	UTSW	4	141,249,590 (GRCm39)	missense	unknown
R8557:Spen	UTSW	4	141,197,681 (GRCm39)	missense	probably benign	0.14
R8558:Spen	UTSW	4	141,197,681 (GRCm39)	missense	probably benign	0.14
R8672:Spen	UTSW	4	141,197,681 (GRCm39)	missense	probably benign	0.14
R8673:Spen	UTSW	4	141,197,681 (GRCm39)	missense	probably benign	0.14
R8674:Spen	UTSW	4	141,197,681 (GRCm39)	missense	probably benign	0.14
R8714:Spen	UTSW	4	141,215,314 (GRCm39)	missense	unknown
R8735:Spen	UTSW	4	141,197,129 (GRCm39)	missense	probably benign	0.32
R8762:Spen	UTSW	4	141,200,261 (GRCm39)	missense	probably damaging	1.00
R8877:Spen	UTSW	4	141,199,137 (GRCm39)	nonsense	probably null
R8878:Spen	UTSW	4	141,204,520 (GRCm39)	missense	unknown
R8937:Spen	UTSW	4	141,201,374 (GRCm39)	missense	probably damaging	1.00
R8939:Spen	UTSW	4	141,202,969 (GRCm39)	missense	possibly damaging	0.72
R8968:Spen	UTSW	4	141,197,701 (GRCm39)	missense	probably benign	0.02
R8971:Spen	UTSW	4	141,201,889 (GRCm39)	missense	possibly damaging	0.53
R9016:Spen	UTSW	4	141,200,938 (GRCm39)	missense	probably damaging	1.00
R9072:Spen	UTSW	4	141,203,702 (GRCm39)	missense	unknown
R9073:Spen	UTSW	4	141,203,702 (GRCm39)	missense	unknown
R9120:Spen	UTSW	4	141,200,233 (GRCm39)	missense
R9136:Spen	UTSW	4	141,249,623 (GRCm39)	missense	unknown
R9138:Spen	UTSW	4	141,196,797 (GRCm39)	missense	probably damaging	1.00
R9150:Spen	UTSW	4	141,244,468 (GRCm39)	missense	unknown
R9225:Spen	UTSW	4	141,202,943 (GRCm39)	missense	possibly damaging	0.53
R9492:Spen	UTSW	4	141,199,098 (GRCm39)	missense	probably benign	0.26
R9537:Spen	UTSW	4	141,244,156 (GRCm39)	small deletion	probably benign
R9537:Spen	UTSW	4	141,199,015 (GRCm39)	missense	probably benign	0.15
R9602:Spen	UTSW	4	141,205,183 (GRCm39)	missense	unknown
R9609:Spen	UTSW	4	141,215,419 (GRCm39)	missense	unknown
R9686:Spen	UTSW	4	141,199,946 (GRCm39)	missense	probably benign	0.27
R9697:Spen	UTSW	4	141,196,275 (GRCm39)	missense	probably damaging	1.00
R9713:Spen	UTSW	4	141,244,331 (GRCm39)	missense	unknown
T0722:Spen	UTSW	4	141,201,664 (GRCm39)	missense	probably benign	0.33
T0975:Spen	UTSW	4	141,201,664 (GRCm39)	missense	probably benign	0.33
Z1088:Spen	UTSW	4	141,205,287 (GRCm39)	missense	unknown
Z1088:Spen	UTSW	4	141,205,288 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- ACAATGACCAACCTGATTGGAGCC -3'
(R):5'- GCTAAGCAGAAGTACCCCATCGTG -3'

Sequencing Primer
(F):5'- GTCCTAACTAAGTGCCCTAAGGTG -3'
(R):5'- TACCCCATCGTGTGGCAG -3'

Posted On

2013-05-09