|Institutional Source||Beutler Lab|
|Gene Name||zinc fingers and homeoboxes 2|
|Synonyms||Afr1, Raf, Afr-1|
|Is this an essential gene?||Possibly non essential (E-score: 0.449)|
|Stock #||R0105 (G1)|
|Chromosomal Location||57694665-57839832 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to C at 57822695 bp|
|Amino Acid Change||Phenylalanine to Leucine at position 487 (F487L)|
|Ref Sequence||ENSEMBL: ENSMUSP00000094164 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000096430]|
|Predicted Effect||probably damaging
AA Change: F487L
PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
AA Change: F487L
|Predicted Effect||noncoding transcript
|Meta Mutation Damage Score||0.9654|
|Coding Region Coverage||
|Validation Efficiency||100% (70/70)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The members of the zinc fingers and homeoboxes gene family are nuclear homodimeric transcriptional repressors that interact with the A subunit of nuclear factor-Y (NF-YA) and contain two C2H2-type zinc fingers and five homeobox DNA-binding domains. This gene encodes member 2 of this gene family. In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family. [provided by RefSeq, Jul 2008]
PHENOTYPE: Alpha-fetoprotein levels in plasma decline precipitously after birth. This gene regulates a difference in adult level and rate of neonatal decrease of AFP. The BALB/cJ substrain carries a genetic variant allele determining a slow rate of decline to adultlevel. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Zhx2||
(F):5'- AAGAGACCTCTGGTGACTCCTCAAG -3'
(R):5'- TGGAATCCAACACAGCCTGCTC -3'
(F):5'- TCCACACATTGCCCAGGTG -3'
(R):5'- TCTCTGAGAACCAGGAGTCG -3'