Mutagenetix > Incidental Mutation

Incidental Mutation 'R4515:Or7g12'

332859

Institutional Source

Beutler Lab

Gene Symbol

Or7g12

Ensembl Gene

ENSMUSG00000095525

Gene Name

olfactory receptor family 7 subfamily G member 12

Synonyms

GA_x6K02T2PVTD-12724921-12725859, MOR153-2, Olfr834, MOR153-4_p

MMRRC Submission

041589-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.471) question?

Stock #

R4515 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

18987990-18988928 bp(+) (GRCm38)

Type of Mutation

splice site

DNA Base Change (assembly)

T to G at 18987982 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000083680 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086492]

AlphaFold

Q7TRG8

Predicted Effect

probably null
Transcript: ENSMUST00000086492

SMART Domains

Protein: ENSMUSP00000083680
Gene: ENSMUSG00000095525

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	7.2e-51	PFAM
Pfam:7TM_GPCR_Srsx	35	305	7e-6	PFAM
Pfam:7tm_1	41	290	2.9e-20	PFAM

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.5%
3x: 98.7%
10x: 97.1%
20x: 94.6%

Validation Efficiency

96% (65/68)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A430033K04Rik	G	T	5: 138,647,744 (GRCm38)	K630N	probably damaging	Het
Acss2	C	A	2: 155,556,363 (GRCm38)	L335I	probably benign	Het
Alg6	A	T	4: 99,752,786 (GRCm38)		probably benign	Het
Atp8b3	C	T	10: 80,523,847 (GRCm38)	M984I	probably benign	Het
Bsn	G	T	9: 108,104,078 (GRCm38)		probably null	Het
Ccdc88a	T	C	11: 29,482,651 (GRCm38)	I1219T	probably benign	Het
Cdhr4	G	A	9: 107,992,951 (GRCm38)	E52K	probably benign	Het
Ces1a	T	A	8: 93,020,904 (GRCm38)	N500Y	probably damaging	Het
Chd3	G	T	11: 69,349,877 (GRCm38)	R1579S	probably benign	Het
Chrnb3	T	C	8: 27,385,090 (GRCm38)	L39P	probably damaging	Het
Ckm	A	G	7: 19,420,284 (GRCm38)	K319E	probably damaging	Het
Cnot6	C	T	11: 49,702,536 (GRCm38)		probably null	Het
Copg1	A	G	6: 87,907,546 (GRCm38)		probably benign	Het
Dbn1	A	T	13: 55,476,229 (GRCm38)	I350N	possibly damaging	Het
Dnah2	A	G	11: 69,465,631 (GRCm38)	S2135P	possibly damaging	Het
Dsg2	C	A	18: 20,601,387 (GRCm38)	D807E	probably benign	Het
Foxi1	A	G	11: 34,207,972 (GRCm38)	F18L	probably damaging	Het
Galnt3	C	A	2: 66,093,610 (GRCm38)	R438L	probably damaging	Het
Glod4	T	A	11: 76,243,571 (GRCm38)	D25V	probably damaging	Het
Gm11492	A	G	11: 87,568,057 (GRCm38)	H419R	probably benign	Het
Golga4	T	A	9: 118,559,008 (GRCm38)	S1733T	probably benign	Het
H2-M10.3	C	T	17: 36,367,830 (GRCm38)		probably null	Het
Hemgn	T	C	4: 46,396,477 (GRCm38)	E253G	probably damaging	Het
Itsn1	G	A	16: 91,899,649 (GRCm38)	V47M	probably damaging	Het
Kif5b	A	G	18: 6,208,257 (GRCm38)	V947A	probably benign	Het
Kif9	A	G	9: 110,489,867 (GRCm38)	H133R	probably benign	Het
Lce1l	T	C	3: 92,850,474 (GRCm38)	T26A	unknown	Het
Macf1	T	C	4: 123,493,988 (GRCm38)	E1172G	probably damaging	Het
Nkx3-2	C	T	5: 41,763,938 (GRCm38)	V3M	probably damaging	Het
Nr2e1	G	A	10: 42,578,191 (GRCm38)	T49I	probably benign	Het
Oc90	A	G	15: 65,892,393 (GRCm38)	L138P	probably damaging	Het
Or8g34	C	T	9: 39,462,231 (GRCm38)	R264*	probably null	Het
Orc4	G	A	2: 48,937,489 (GRCm38)	P31S	probably benign	Het
Pabir3	G	A	X: 53,293,499 (GRCm38)	R94H	possibly damaging	Het
Paqr3	T	A	5: 97,103,361 (GRCm38)	N168I	possibly damaging	Het
Pcdhac1	T	A	18: 37,091,379 (GRCm38)	I415N	probably damaging	Het
Pdik1l	T	C	4: 134,278,896 (GRCm38)	N75S	probably damaging	Het
Pik3r4	A	G	9: 105,672,725 (GRCm38)	H1005R	probably damaging	Het
Plekhn1	T	C	4: 156,225,531 (GRCm38)	S109G	probably damaging	Het
Prkch	A	G	12: 73,702,838 (GRCm38)	T402A	possibly damaging	Het
Ptger4	C	A	15: 5,242,379 (GRCm38)	R253L	probably damaging	Het
Rabac1	A	T	7: 24,970,160 (GRCm38)	Y173*	probably null	Het
Rapsn	G	A	2: 91,043,212 (GRCm38)	V288M	possibly damaging	Het
Rnf26rt	C	T	6: 76,496,809 (GRCm38)	V275I	probably benign	Het
Sec31a	A	G	5: 100,365,958 (GRCm38)	S993P	probably damaging	Het
Serpina9	T	A	12: 104,001,294 (GRCm38)	M281L	probably benign	Het
Serpine1	G	A	5: 137,069,468 (GRCm38)	A117V	probably damaging	Het
Sh3tc2	A	G	18: 61,987,693 (GRCm38)		probably null	Het
Sipa1l2	T	C	8: 125,492,226 (GRCm38)	D124G	probably benign	Het
Slc6a12	T	A	6: 121,353,530 (GRCm38)		probably null	Het
Stk11	A	G	10: 80,116,601 (GRCm38)		probably benign	Het
Tcaf3	A	G	6: 42,589,996 (GRCm38)	Y720H	probably damaging	Het
Tmem38a	C	T	8: 72,572,161 (GRCm38)	P20S	possibly damaging	Het
Tmprss11a	C	T	5: 86,420,196 (GRCm38)	R224K	probably damaging	Het
Tmprss15	C	T	16: 78,957,356 (GRCm38)	S988N	probably benign	Het
Txn2	A	T	15: 77,915,443 (GRCm38)		probably null	Het
Ugt1a10	T	A	1: 88,056,197 (GRCm38)	V239E	probably damaging	Het
Vmn2r102	A	G	17: 19,681,213 (GRCm38)	Y534C	probably damaging	Het
Vmn2r78	A	T	7: 86,954,258 (GRCm38)	D548V	probably damaging	Het

Other mutations in Or7g12

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01804:Or7g12	APN	9	18,988,840 (GRCm38)	missense	probably benign	0.16
IGL02073:Or7g12	APN	9	18,988,325 (GRCm38)	missense	possibly damaging	0.89
IGL02119:Or7g12	APN	9	18,988,612 (GRCm38)	missense	probably benign	0.00
IGL02705:Or7g12	APN	9	18,988,400 (GRCm38)	missense	probably benign	0.03
R0462:Or7g12	UTSW	9	18,988,902 (GRCm38)	missense	probably benign
R0466:Or7g12	UTSW	9	18,988,255 (GRCm38)	missense	probably benign	0.00
R0709:Or7g12	UTSW	9	18,988,126 (GRCm38)	missense	probably damaging	0.98
R0711:Or7g12	UTSW	9	18,988,151 (GRCm38)	missense	probably benign	0.04
R1268:Or7g12	UTSW	9	18,988,356 (GRCm38)	missense	probably damaging	0.98
R1663:Or7g12	UTSW	9	18,988,710 (GRCm38)	missense	probably damaging	0.99
R1680:Or7g12	UTSW	9	18,988,516 (GRCm38)	missense	possibly damaging	0.81
R1686:Or7g12	UTSW	9	18,988,543 (GRCm38)	missense	probably damaging	1.00
R1903:Or7g12	UTSW	9	18,988,896 (GRCm38)	nonsense	probably null
R1907:Or7g12	UTSW	9	18,988,441 (GRCm38)	missense	possibly damaging	0.82
R1911:Or7g12	UTSW	9	18,988,900 (GRCm38)	missense	probably damaging	0.99
R2143:Or7g12	UTSW	9	18,988,803 (GRCm38)	missense	probably benign	0.06
R2431:Or7g12	UTSW	9	18,988,003 (GRCm38)	missense	probably damaging	1.00
R4014:Or7g12	UTSW	9	18,988,882 (GRCm38)	missense	probably benign	0.08
R4575:Or7g12	UTSW	9	18,988,705 (GRCm38)	nonsense	probably null
R6974:Or7g12	UTSW	9	18,988,393 (GRCm38)	missense	probably damaging	0.99
R7394:Or7g12	UTSW	9	18,988,710 (GRCm38)	missense	probably damaging	0.99
R7455:Or7g12	UTSW	9	18,988,854 (GRCm38)	missense	possibly damaging	0.92
R7828:Or7g12	UTSW	9	18,988,920 (GRCm38)	missense	probably benign
R7962:Or7g12	UTSW	9	18,988,656 (GRCm38)	missense	probably damaging	0.97
R8360:Or7g12	UTSW	9	18,988,843 (GRCm38)	missense	probably benign	0.28
R8812:Or7g12	UTSW	9	18,988,516 (GRCm38)	missense	possibly damaging	0.81
R8905:Or7g12	UTSW	9	18,988,198 (GRCm38)	missense	possibly damaging	0.92
R8973:Or7g12	UTSW	9	18,988,678 (GRCm38)	nonsense	probably null
R8980:Or7g12	UTSW	9	18,988,127 (GRCm38)	missense	probably damaging	1.00
R9013:Or7g12	UTSW	9	18,988,578 (GRCm38)	missense	possibly damaging	0.94
R9058:Or7g12	UTSW	9	18,988,926 (GRCm38)	makesense	probably null
R9614:Or7g12	UTSW	9	18,988,230 (GRCm38)	missense	possibly damaging	0.75
R9779:Or7g12	UTSW	9	18,988,839 (GRCm38)	missense	probably benign	0.04

Predicted Primers

PCR Primer
(F):5'- TATGTGTTGGTTAACCTAGTTCCTC -3'
(R):5'- ACCAGCATTTTGGGGATGG -3'

Sequencing Primer
(F):5'- AAGTAGAATTACTCTGTTCCAACATG -3'
(R):5'- TGGTGGTAGTGAAACATATGTCAATG -3'

Posted On

2015-08-18