Mutagenetix > Incidental Mutation

Incidental Mutation 'R4516:Lama3'

332933

Institutional Source

Beutler Lab

Gene Symbol

Lama3

Ensembl Gene

ENSMUSG00000024421

Gene Name

laminin, alpha 3

Synonyms

[a]3B, nicein, 150kDa

MMRRC Submission

041760-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4516 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

12466876-12716070 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 12628415 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glutamic Acid at position 1502 (D1502E)

Ref Sequence

ENSEMBL: ENSMUSP00000089703 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000092070]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000092070
AA Change: D1502E

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000089703
Gene: ENSMUSG00000024421
AA Change: D1502E

Domain	Start	End	E-Value	Type
signal peptide	1	31	N/A	INTRINSIC
LamNT	38	294	1.46e-153	SMART
EGF_Lam	296	350	1.39e-4	SMART
EGF_Lam	353	420	2.66e-10	SMART
EGF_Lam	423	464	3.51e-10	SMART
EGF_Lam	488	530	1.73e-9	SMART
EGF_Lam	533	576	3.81e-11	SMART
EGF_like	579	625	1.82e-1	SMART
EGF_Lam	628	678	5.15e-8	SMART
EGF_Lam	681	725	3.54e-6	SMART
low complexity region	768	781	N/A	INTRINSIC
EGF_Lam	1263	1306	3.15e-12	SMART
EGF_Lam	1309	1350	6.3e-3	SMART
EGF_Lam	1353	1399	1.49e-13	SMART
EGF_Lam	1402	1450	8.18e-11	SMART
LamB	1509	1638	4.34e-55	SMART
Pfam:Laminin_EGF	1647	1681	7.9e-5	PFAM
EGF_Lam	1684	1728	2.66e-10	SMART
EGF_Lam	1731	1781	7.81e-8	SMART
Pfam:Laminin_I	1836	2102	2.7e-93	PFAM
low complexity region	2185	2200	N/A	INTRINSIC
coiled coil region	2211	2238	N/A	INTRINSIC
LamG	2406	2566	1.67e-2	SMART
LamG	2614	2742	1.72e-17	SMART
LamG	2785	2900	3.96e-17	SMART
LamG	3005	3133	1.12e-34	SMART
LamG	3175	3308	3.41e-30	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000187766

Meta Mutation Damage Score

0.1564

Coding Region Coverage

1x: 99.4%
3x: 98.5%
10x: 96.6%
20x: 92.9%

Validation Efficiency

86% (51/59)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the laminin family of secreted molecules. Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. Laminins are essential for formation and function of the basement membrane and have additional functions in regulating cell migration and mechanical signal transduction. This gene encodes an alpha subunit and is responsive to several epithelial-mesenchymal regulators including keratinocyte growth factor, epidermal growth factor and insulin-like growth factor. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa and laryngoonychocutaneous syndrome. Alternative splicing and alternative promoter usage result in multiple transcript variants. [provided by RefSeq, Dec 2014]
PHENOTYPE: Mice homozygous for a targeted null mutation develop a lethal blistering phenotype similar to human junctional epidermolysis bullosa, and die 2-3 days after birth from a failure to thrive. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Bean1	T	C	8: 104,941,786 (GRCm39)	S211P	probably damaging	Het
Bltp1	T	C	3: 36,949,460 (GRCm39)	S369P	possibly damaging	Het
Camta1	A	G	4: 151,229,177 (GRCm39)	S552P	possibly damaging	Het
Cdh11	T	C	8: 103,400,594 (GRCm39)	T125A	possibly damaging	Het
Cdk5rap2	A	T	4: 70,194,952 (GRCm39)		probably null	Het
Cenpc1	A	G	5: 86,195,446 (GRCm39)	S108P	possibly damaging	Het
Cfap20dc	A	T	14: 8,536,609 (GRCm38)	D199E	probably damaging	Het
Cfap44	T	A	16: 44,294,227 (GRCm39)	Y224*	probably null	Het
Cfap46	G	A	7: 139,239,998 (GRCm39)		probably benign	Het
Cntrl	G	A	2: 35,017,993 (GRCm39)	V468I	probably benign	Het
Col6a6	C	T	9: 105,576,148 (GRCm39)	V2071I	possibly damaging	Het
Coq7	A	T	7: 118,109,130 (GRCm39)	L306Q	unknown	Het
D7Ertd443e	C	G	7: 133,895,057 (GRCm39)	Q591H	probably damaging	Het
Dchs1	C	T	7: 105,404,059 (GRCm39)	V2828M	probably damaging	Het
Dzank1	T	A	2: 144,352,042 (GRCm39)		probably benign	Het
Elmo1	C	T	13: 20,467,084 (GRCm39)	T235I	probably benign	Het
Elp3	A	T	14: 65,785,326 (GRCm39)	F492I	possibly damaging	Het
Espl1	A	G	15: 102,231,671 (GRCm39)	S90G	probably benign	Het
Fbxl20	T	C	11: 97,986,061 (GRCm39)		probably benign	Het
Gm10722	T	C	9: 3,000,937 (GRCm39)	C6R	probably benign	Het
Got1	T	C	19: 43,493,280 (GRCm39)	Y243C	probably damaging	Het
Hipk1	G	T	3: 103,657,688 (GRCm39)	H799N	probably damaging	Het
Kif21a	A	T	15: 90,855,345 (GRCm39)	M673K	probably benign	Het
Limk1	A	G	5: 134,705,640 (GRCm39)		probably benign	Het
Myo5b	A	G	18: 74,758,745 (GRCm39)	Y242C	probably damaging	Het
Naa12	G	A	18: 80,254,791 (GRCm39)	M28I	probably benign	Het
Ncbp1	T	C	4: 46,157,824 (GRCm39)	V354A	probably damaging	Het
Ncoa2	T	C	1: 13,217,130 (GRCm39)	D1380G	probably damaging	Het
Ntng1	A	G	3: 109,842,329 (GRCm39)	I148T	probably damaging	Het
Oas1d	A	T	5: 121,057,233 (GRCm39)	T280S	probably damaging	Het
Or1e29	C	T	11: 73,667,866 (GRCm39)	G96S	probably benign	Het
Or4c15b	T	A	2: 89,113,187 (GRCm39)	M118L	probably benign	Het
Pax7	T	G	4: 139,508,104 (GRCm39)	D307A	probably benign	Het
Pdxdc1	G	A	16: 13,656,210 (GRCm39)	Q621*	probably null	Het
Rab29	A	T	1: 131,795,469 (GRCm39)	Y27F	possibly damaging	Het
Rab3gap2	G	A	1: 184,999,265 (GRCm39)	V991I	probably benign	Het
Ric1	A	G	19: 29,548,165 (GRCm39)	T278A	probably benign	Het
Rufy4	T	C	1: 74,186,822 (GRCm39)	C537R	probably damaging	Het
Slc47a1	T	C	11: 61,235,339 (GRCm39)	H498R	probably benign	Het
Spmip6	T	C	4: 41,517,200 (GRCm39)		probably benign	Het
Tas2r116	T	C	6: 132,833,113 (GRCm39)	L238P	probably damaging	Het
Tigd5	A	T	15: 75,782,364 (GRCm39)	R252*	probably null	Het
Tlr6	A	G	5: 65,112,247 (GRCm39)	F220S	possibly damaging	Het
Tmem106b	C	T	6: 13,075,098 (GRCm39)	T95I	probably damaging	Het
Ubqlnl	C	T	7: 103,798,925 (GRCm39)	V191M	probably benign	Het
Vmn1r174	A	G	7: 23,453,768 (GRCm39)	I145V	probably benign	Het
Vps26a	T	C	10: 62,304,124 (GRCm39)	M116V	probably damaging	Het

Other mutations in Lama3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00091:Lama3	APN	18	12,713,349 (GRCm39)	missense	probably benign
IGL00272:Lama3	APN	18	12,624,605 (GRCm39)	missense	probably damaging	1.00
IGL00335:Lama3	APN	18	12,582,645 (GRCm39)	splice site	probably benign
IGL00836:Lama3	APN	18	12,605,285 (GRCm39)	missense	probably benign	0.01
IGL01017:Lama3	APN	18	12,574,200 (GRCm39)	critical splice donor site	probably null
IGL01025:Lama3	APN	18	12,614,094 (GRCm39)	missense	probably benign	0.09
IGL01394:Lama3	APN	18	12,664,983 (GRCm39)	missense	probably null	0.39
IGL01545:Lama3	APN	18	12,574,188 (GRCm39)	missense	probably benign	0.01
IGL01685:Lama3	APN	18	12,586,937 (GRCm39)	splice site	probably benign
IGL01863:Lama3	APN	18	12,552,993 (GRCm39)	splice site	probably benign
IGL01869:Lama3	APN	18	12,657,820 (GRCm39)	missense	possibly damaging	0.94
IGL01894:Lama3	APN	18	12,705,121 (GRCm39)	missense	probably benign	0.09
IGL02027:Lama3	APN	18	12,649,570 (GRCm39)	missense	probably damaging	1.00
IGL02106:Lama3	APN	18	12,601,371 (GRCm39)	missense	probably damaging	0.98
IGL02307:Lama3	APN	18	12,714,840 (GRCm39)	missense	probably benign	0.09
IGL02342:Lama3	APN	18	12,624,533 (GRCm39)	missense	probably damaging	1.00
IGL02377:Lama3	APN	18	12,689,807 (GRCm39)	missense	possibly damaging	0.49
IGL02401:Lama3	APN	18	12,690,784 (GRCm39)	missense	probably benign	0.02
IGL02517:Lama3	APN	18	12,670,915 (GRCm39)	critical splice donor site	probably null
IGL02644:Lama3	APN	18	12,658,910 (GRCm39)	missense	probably benign	0.12
IGL02733:Lama3	APN	18	12,711,184 (GRCm39)	missense	probably damaging	0.99
IGL02932:Lama3	APN	18	12,661,858 (GRCm39)	missense	probably damaging	1.00
IGL03006:Lama3	APN	18	12,601,425 (GRCm39)	splice site	probably benign
IGL03038:Lama3	APN	18	12,552,307 (GRCm39)	missense	probably damaging	0.99
IGL03064:Lama3	APN	18	12,572,406 (GRCm39)	missense	possibly damaging	0.72
IGL03146:Lama3	APN	18	12,660,681 (GRCm39)	missense	possibly damaging	0.66
IGL03233:Lama3	APN	18	12,614,095 (GRCm39)	missense	probably damaging	1.00
IGL03255:Lama3	APN	18	12,672,760 (GRCm39)	missense	probably damaging	1.00
IGL03369:Lama3	APN	18	12,686,340 (GRCm39)	missense	probably benign	0.05
IGL03412:Lama3	APN	18	12,552,239 (GRCm39)	missense	probably damaging	0.99
IGL02980:Lama3	UTSW	18	12,686,288 (GRCm39)	missense	probably benign	0.01
IGL03014:Lama3	UTSW	18	12,673,024 (GRCm39)	missense	possibly damaging	0.95
R0007:Lama3	UTSW	18	12,630,938 (GRCm39)	splice site	probably benign
R0007:Lama3	UTSW	18	12,630,938 (GRCm39)	splice site	probably benign
R0050:Lama3	UTSW	18	12,537,160 (GRCm39)	missense	probably damaging	1.00
R0050:Lama3	UTSW	18	12,537,160 (GRCm39)	missense	probably damaging	1.00
R0063:Lama3	UTSW	18	12,661,762 (GRCm39)	splice site	probably benign
R0063:Lama3	UTSW	18	12,661,762 (GRCm39)	splice site	probably benign
R0106:Lama3	UTSW	18	12,537,039 (GRCm39)	missense	probably damaging	0.96
R0148:Lama3	UTSW	18	12,581,329 (GRCm39)	missense	probably damaging	1.00
R0165:Lama3	UTSW	18	12,657,867 (GRCm39)	missense	probably damaging	0.99
R0240:Lama3	UTSW	18	12,672,880 (GRCm39)	splice site	probably null
R0240:Lama3	UTSW	18	12,672,880 (GRCm39)	splice site	probably null
R0316:Lama3	UTSW	18	12,652,934 (GRCm39)	missense	probably benign	0.09
R0325:Lama3	UTSW	18	12,615,183 (GRCm39)	missense	probably damaging	1.00
R0365:Lama3	UTSW	18	12,640,064 (GRCm39)	missense	probably damaging	0.96
R0390:Lama3	UTSW	18	12,540,620 (GRCm39)	missense	probably benign	0.10
R0408:Lama3	UTSW	18	12,589,894 (GRCm39)	missense	probably benign
R0449:Lama3	UTSW	18	12,633,569 (GRCm39)	splice site	probably null
R0453:Lama3	UTSW	18	12,598,535 (GRCm39)	missense	possibly damaging	0.63
R0480:Lama3	UTSW	18	12,583,481 (GRCm39)	missense	possibly damaging	0.81
R0536:Lama3	UTSW	18	12,658,951 (GRCm39)	missense	probably damaging	1.00
R0545:Lama3	UTSW	18	12,694,758 (GRCm39)	missense	possibly damaging	0.90
R0567:Lama3	UTSW	18	12,682,309 (GRCm39)	missense	probably benign
R0605:Lama3	UTSW	18	12,640,006 (GRCm39)	missense	probably benign	0.02
R0617:Lama3	UTSW	18	12,552,315 (GRCm39)	critical splice donor site	probably null
R0629:Lama3	UTSW	18	12,552,302 (GRCm39)	missense	possibly damaging	0.79
R0671:Lama3	UTSW	18	12,610,647 (GRCm39)	missense	possibly damaging	0.80
R0730:Lama3	UTSW	18	12,589,907 (GRCm39)	splice site	probably benign
R1216:Lama3	UTSW	18	12,554,191 (GRCm39)	splice site	probably benign
R1356:Lama3	UTSW	18	12,633,634 (GRCm39)	unclassified	probably benign
R1386:Lama3	UTSW	18	12,610,427 (GRCm39)	missense	probably benign	0.04
R1424:Lama3	UTSW	18	12,653,048 (GRCm39)	missense	probably benign	0.13
R1426:Lama3	UTSW	18	12,614,155 (GRCm39)	critical splice donor site	probably null
R1437:Lama3	UTSW	18	12,682,284 (GRCm39)	missense	possibly damaging	0.46
R1468:Lama3	UTSW	18	12,574,164 (GRCm39)	missense	probably benign	0.00
R1468:Lama3	UTSW	18	12,574,164 (GRCm39)	missense	probably benign	0.00
R1472:Lama3	UTSW	18	12,615,102 (GRCm39)	missense	probably benign	0.23
R1557:Lama3	UTSW	18	12,646,788 (GRCm39)	splice site	probably benign
R1571:Lama3	UTSW	18	12,672,774 (GRCm39)	missense	probably damaging	0.98
R1599:Lama3	UTSW	18	12,583,457 (GRCm39)	nonsense	probably null
R1631:Lama3	UTSW	18	12,540,551 (GRCm39)	missense	probably damaging	1.00
R1647:Lama3	UTSW	18	12,665,256 (GRCm39)	missense	possibly damaging	0.90
R1648:Lama3	UTSW	18	12,665,256 (GRCm39)	missense	possibly damaging	0.90
R1719:Lama3	UTSW	18	12,612,929 (GRCm39)	critical splice donor site	probably null
R1757:Lama3	UTSW	18	12,598,556 (GRCm39)	missense	probably benign	0.10
R1766:Lama3	UTSW	18	12,535,119 (GRCm39)	missense	probably damaging	1.00
R1853:Lama3	UTSW	18	12,646,762 (GRCm39)	missense	possibly damaging	0.75
R1856:Lama3	UTSW	18	12,670,838 (GRCm39)	nonsense	probably null
R1909:Lama3	UTSW	18	12,714,855 (GRCm39)	missense	probably benign	0.19
R1913:Lama3	UTSW	18	12,628,336 (GRCm39)	missense	probably benign	0.15
R1975:Lama3	UTSW	18	12,586,920 (GRCm39)	missense	probably damaging	1.00
R2014:Lama3	UTSW	18	12,657,778 (GRCm39)	splice site	probably benign
R2059:Lama3	UTSW	18	12,661,390 (GRCm39)	missense	probably damaging	0.98
R2060:Lama3	UTSW	18	12,661,783 (GRCm39)	missense	probably benign	0.30
R2086:Lama3	UTSW	18	12,657,887 (GRCm39)	missense	probably benign	0.39
R2115:Lama3	UTSW	18	12,535,906 (GRCm39)	missense	possibly damaging	0.94
R2291:Lama3	UTSW	18	12,658,136 (GRCm39)	missense	probably damaging	0.98
R2860:Lama3	UTSW	18	12,586,807 (GRCm39)	missense	probably damaging	1.00
R2861:Lama3	UTSW	18	12,586,807 (GRCm39)	missense	probably damaging	1.00
R2862:Lama3	UTSW	18	12,586,807 (GRCm39)	missense	probably damaging	1.00
R3410:Lama3	UTSW	18	12,546,915 (GRCm39)	critical splice donor site	probably null
R3614:Lama3	UTSW	18	12,581,345 (GRCm39)	missense	probably benign	0.03
R3696:Lama3	UTSW	18	12,572,532 (GRCm39)	splice site	probably benign
R3752:Lama3	UTSW	18	12,640,086 (GRCm39)	missense	probably damaging	1.00
R3967:Lama3	UTSW	18	12,713,398 (GRCm39)	missense	probably damaging	1.00
R3968:Lama3	UTSW	18	12,713,398 (GRCm39)	missense	probably damaging	1.00
R3969:Lama3	UTSW	18	12,713,398 (GRCm39)	missense	probably damaging	1.00
R3970:Lama3	UTSW	18	12,713,398 (GRCm39)	missense	probably damaging	1.00
R4088:Lama3	UTSW	18	12,637,365 (GRCm39)	nonsense	probably null
R4118:Lama3	UTSW	18	12,583,488 (GRCm39)	missense	probably benign	0.01
R4222:Lama3	UTSW	18	12,583,460 (GRCm39)	missense	probably damaging	1.00
R4223:Lama3	UTSW	18	12,583,460 (GRCm39)	missense	probably damaging	1.00
R4224:Lama3	UTSW	18	12,583,460 (GRCm39)	missense	probably damaging	1.00
R4225:Lama3	UTSW	18	12,583,460 (GRCm39)	missense	probably damaging	1.00
R4367:Lama3	UTSW	18	12,646,747 (GRCm39)	missense	probably damaging	1.00
R4404:Lama3	UTSW	18	12,715,588 (GRCm39)	missense	probably benign	0.01
R4424:Lama3	UTSW	18	12,652,929 (GRCm39)	nonsense	probably null
R4483:Lama3	UTSW	18	12,682,310 (GRCm39)	missense	probably benign	0.32
R4484:Lama3	UTSW	18	12,614,145 (GRCm39)	missense	probably benign
R4556:Lama3	UTSW	18	12,612,816 (GRCm39)	missense	possibly damaging	0.63
R4616:Lama3	UTSW	18	12,637,454 (GRCm39)	critical splice donor site	probably null
R4702:Lama3	UTSW	18	12,711,086 (GRCm39)	nonsense	probably null
R4704:Lama3	UTSW	18	12,686,280 (GRCm39)	missense	probably benign	0.08
R4750:Lama3	UTSW	18	12,637,416 (GRCm39)	missense	probably benign	0.25
R4753:Lama3	UTSW	18	12,615,141 (GRCm39)	missense	probably damaging	1.00
R4767:Lama3	UTSW	18	12,633,620 (GRCm39)	missense	probably benign	0.32
R4777:Lama3	UTSW	18	12,546,828 (GRCm39)	missense	probably damaging	1.00
R4782:Lama3	UTSW	18	12,544,627 (GRCm39)	nonsense	probably null
R4784:Lama3	UTSW	18	12,582,601 (GRCm39)	missense	probably benign	0.20
R4816:Lama3	UTSW	18	12,610,661 (GRCm39)	missense	possibly damaging	0.93
R4833:Lama3	UTSW	18	12,574,188 (GRCm39)	missense	probably benign	0.01
R4854:Lama3	UTSW	18	12,544,599 (GRCm39)	missense	probably benign	0.00
R4863:Lama3	UTSW	18	12,631,735 (GRCm39)	intron	probably benign
R4863:Lama3	UTSW	18	12,672,850 (GRCm39)	missense	probably damaging	0.99
R4953:Lama3	UTSW	18	12,581,362 (GRCm39)	missense	probably damaging	1.00
R4974:Lama3	UTSW	18	12,685,883 (GRCm39)	missense	probably damaging	0.98
R4996:Lama3	UTSW	18	12,651,800 (GRCm39)	missense	probably benign	0.24
R5049:Lama3	UTSW	18	12,715,668 (GRCm39)	missense	probably benign	0.19
R5057:Lama3	UTSW	18	12,665,005 (GRCm39)	missense	probably null	0.82
R5090:Lama3	UTSW	18	12,675,459 (GRCm39)	missense	possibly damaging	0.94
R5122:Lama3	UTSW	18	12,672,823 (GRCm39)	missense	possibly damaging	0.53
R5215:Lama3	UTSW	18	12,710,957 (GRCm39)	missense	probably damaging	1.00
R5245:Lama3	UTSW	18	12,552,950 (GRCm39)	missense	probably damaging	1.00
R5259:Lama3	UTSW	18	12,598,565 (GRCm39)	missense	probably damaging	1.00
R5320:Lama3	UTSW	18	12,685,912 (GRCm39)	missense	probably damaging	0.99
R5377:Lama3	UTSW	18	12,586,803 (GRCm39)	missense	probably damaging	0.99
R5432:Lama3	UTSW	18	12,705,123 (GRCm39)	missense	probably damaging	1.00
R5500:Lama3	UTSW	18	12,589,821 (GRCm39)	missense	possibly damaging	0.93
R5534:Lama3	UTSW	18	12,686,267 (GRCm39)	missense	probably benign	0.00
R5589:Lama3	UTSW	18	12,605,277 (GRCm39)	missense	possibly damaging	0.46
R5604:Lama3	UTSW	18	12,572,405 (GRCm39)	missense	probably benign
R5617:Lama3	UTSW	18	12,631,993 (GRCm39)	intron	probably benign
R5709:Lama3	UTSW	18	12,672,856 (GRCm39)	missense	probably damaging	1.00
R5965:Lama3	UTSW	18	12,562,944 (GRCm39)	missense	possibly damaging	0.67
R6042:Lama3	UTSW	18	12,707,311 (GRCm39)	missense	probably damaging	1.00
R6065:Lama3	UTSW	18	12,602,985 (GRCm39)	missense	possibly damaging	0.53
R6085:Lama3	UTSW	18	12,615,156 (GRCm39)	missense	probably benign	0.01
R6212:Lama3	UTSW	18	12,646,702 (GRCm39)	missense	probably damaging	1.00
R6268:Lama3	UTSW	18	12,657,794 (GRCm39)	missense	probably damaging	0.98
R6276:Lama3	UTSW	18	12,640,006 (GRCm39)	missense	probably benign	0.02
R6366:Lama3	UTSW	18	12,615,194 (GRCm39)	missense	probably damaging	1.00
R6393:Lama3	UTSW	18	12,612,813 (GRCm39)	missense	probably benign	0.44
R6493:Lama3	UTSW	18	12,615,205 (GRCm39)	critical splice donor site	probably null
R6505:Lama3	UTSW	18	12,628,405 (GRCm39)	missense	probably benign	0.02
R6563:Lama3	UTSW	18	12,670,823 (GRCm39)	missense	probably damaging	1.00
R6582:Lama3	UTSW	18	12,710,897 (GRCm39)	missense	probably damaging	1.00
R6585:Lama3	UTSW	18	12,552,314 (GRCm39)	critical splice donor site	probably null
R6609:Lama3	UTSW	18	12,646,735 (GRCm39)	missense	probably damaging	0.99
R6656:Lama3	UTSW	18	12,682,283 (GRCm39)	missense	possibly damaging	0.66
R6833:Lama3	UTSW	18	12,624,605 (GRCm39)	missense	probably damaging	1.00
R6834:Lama3	UTSW	18	12,624,605 (GRCm39)	missense	probably damaging	1.00
R7019:Lama3	UTSW	18	12,661,475 (GRCm39)	missense	probably damaging	0.97
R7026:Lama3	UTSW	18	12,649,605 (GRCm39)	missense	probably damaging	0.98
R7088:Lama3	UTSW	18	12,715,602 (GRCm39)	missense	possibly damaging	0.90
R7100:Lama3	UTSW	18	12,715,701 (GRCm39)	missense	possibly damaging	0.80
R7102:Lama3	UTSW	18	12,685,870 (GRCm39)	missense	possibly damaging	0.66
R7103:Lama3	UTSW	18	12,664,936 (GRCm39)	missense	probably benign	0.00
R7121:Lama3	UTSW	18	12,595,839 (GRCm39)	missense	probably benign	0.06
R7133:Lama3	UTSW	18	12,672,843 (GRCm39)	missense	probably benign	0.05
R7150:Lama3	UTSW	18	12,601,346 (GRCm39)	missense	probably damaging	1.00
R7158:Lama3	UTSW	18	12,589,869 (GRCm39)	missense	probably benign	0.20
R7170:Lama3	UTSW	18	12,537,133 (GRCm39)	missense	probably benign	0.26
R7216:Lama3	UTSW	18	12,563,057 (GRCm39)	missense	probably damaging	1.00
R7223:Lama3	UTSW	18	12,715,665 (GRCm39)	missense	possibly damaging	0.53
R7243:Lama3	UTSW	18	12,552,902 (GRCm39)	missense	probably damaging	1.00
R7282:Lama3	UTSW	18	12,572,449 (GRCm39)	missense	probably damaging	0.99
R7337:Lama3	UTSW	18	12,640,097 (GRCm39)	splice site	probably null
R7442:Lama3	UTSW	18	12,605,238 (GRCm39)	critical splice acceptor site	probably null
R7487:Lama3	UTSW	18	12,552,294 (GRCm39)	missense	probably benign
R7604:Lama3	UTSW	18	12,633,550 (GRCm39)	missense	possibly damaging	0.93
R7609:Lama3	UTSW	18	12,664,891 (GRCm39)	critical splice acceptor site	probably null
R7650:Lama3	UTSW	18	12,670,895 (GRCm39)	missense	probably benign	0.01
R7894:Lama3	UTSW	18	12,595,864 (GRCm39)	missense	probably benign	0.07
R7975:Lama3	UTSW	18	12,670,796 (GRCm39)	missense	probably damaging	1.00
R8099:Lama3	UTSW	18	12,667,120 (GRCm39)	missense	probably damaging	0.97
R8168:Lama3	UTSW	18	12,639,999 (GRCm39)	missense	probably null
R8219:Lama3	UTSW	18	12,572,417 (GRCm39)	missense	probably benign	0.07
R8227:Lama3	UTSW	18	12,540,608 (GRCm39)	missense	probably benign
R8229:Lama3	UTSW	18	12,540,608 (GRCm39)	missense	probably benign
R8298:Lama3	UTSW	18	12,658,910 (GRCm39)	missense	probably benign	0.12
R8351:Lama3	UTSW	18	12,673,670 (GRCm39)	missense	probably damaging	1.00
R8364:Lama3	UTSW	18	12,661,404 (GRCm39)	missense	probably damaging	0.99
R8463:Lama3	UTSW	18	12,582,896 (GRCm39)	missense	probably damaging	0.96
R8515:Lama3	UTSW	18	12,544,688 (GRCm39)	missense	probably null	0.01
R8784:Lama3	UTSW	18	12,554,212 (GRCm39)	missense	probably benign
R8799:Lama3	UTSW	18	12,624,000 (GRCm39)	missense	probably damaging	0.96
R8874:Lama3	UTSW	18	12,582,643 (GRCm39)	critical splice donor site	probably null
R8938:Lama3	UTSW	18	12,689,762 (GRCm39)	missense	probably damaging	1.00
R8967:Lama3	UTSW	18	12,665,096 (GRCm39)	missense	possibly damaging	0.46
R9039:Lama3	UTSW	18	12,614,120 (GRCm39)	nonsense	probably null
R9126:Lama3	UTSW	18	12,583,527 (GRCm39)	missense	probably damaging	1.00
R9200:Lama3	UTSW	18	12,605,297 (GRCm39)	missense	probably benign	0.00
R9203:Lama3	UTSW	18	12,595,869 (GRCm39)	missense	probably benign	0.04
R9246:Lama3	UTSW	18	12,710,959 (GRCm39)	missense	probably damaging	0.99
R9284:Lama3	UTSW	18	12,583,541 (GRCm39)	nonsense	probably null
R9553:Lama3	UTSW	18	12,563,019 (GRCm39)	missense	probably damaging	1.00
R9716:Lama3	UTSW	18	12,583,460 (GRCm39)	missense	probably damaging	1.00
R9734:Lama3	UTSW	18	12,682,320 (GRCm39)	missense	possibly damaging	0.94
X0019:Lama3	UTSW	18	12,715,631 (GRCm39)	missense	possibly damaging	0.94
Z1177:Lama3	UTSW	18	12,562,936 (GRCm39)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- ATAGGAATGGCCCCTTGAGG -3'
(R):5'- ACCTGAACTTCCAGATGCC -3'

Sequencing Primer
(F):5'- TGTATTCCACACGAGTGCG -3'
(R):5'- GAACTTCCAGATGCCTCCTC -3'

Posted On

2015-08-18