Incidental Mutation 'R4517:Rufy4'
ID 332938
Institutional Source Beutler Lab
Gene Symbol Rufy4
Ensembl Gene ENSMUSG00000061815
Gene Name RUN and FYVE domain containing 4
Synonyms F930048N03Rik
MMRRC Submission 041761-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.194) question?
Stock # R4517 (G1)
Quality Score 225
Status Validated
Chromosome 1
Chromosomal Location 74164700-74187382 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 74186822 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Cysteine to Arginine at position 537 (C537R)
Ref Sequence ENSEMBL: ENSMUSP00000115873 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000080167] [ENSMUST00000127134]
AlphaFold Q3TYX8
Predicted Effect probably damaging
Transcript: ENSMUST00000080167
AA Change: C453R

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)
SMART Domains Protein: ENSMUSP00000079062
Gene: ENSMUSG00000061815
AA Change: C453R

DomainStartEndE-ValueType
Pfam:RUN 2 81 1.5e-8 PFAM
coiled coil region 331 404 N/A INTRINSIC
Blast:FYVE 415 472 2e-6 BLAST
SCOP:d1vfya_ 428 473 4e-7 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000127134
AA Change: C537R

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)
SMART Domains Protein: ENSMUSP00000115873
Gene: ENSMUSG00000061815
AA Change: C537R

DomainStartEndE-ValueType
Pfam:RUN 41 165 6.2e-10 PFAM
coiled coil region 415 488 N/A INTRINSIC
Blast:FYVE 499 556 2e-6 BLAST
SCOP:d1vfya_ 512 557 3e-7 SMART
Meta Mutation Damage Score 0.8605 question?
Coding Region Coverage
  • 1x: 99.4%
  • 3x: 98.5%
  • 10x: 96.7%
  • 20x: 93.6%
Validation Efficiency 95% (39/41)
Allele List at MGI
Other mutations in this stock
Total: 32 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Ank A G 15: 27,562,835 (GRCm39) H181R possibly damaging Het
B4galnt4 A G 7: 140,647,635 (GRCm39) K408E probably damaging Het
Cd209a T A 8: 3,795,525 (GRCm39) D123V probably damaging Het
Cyp4f37 A G 17: 32,850,566 (GRCm39) I340V probably benign Het
Echs1 A G 7: 139,692,409 (GRCm39) S113P possibly damaging Het
Fap T C 2: 62,361,059 (GRCm39) I391V probably benign Het
Glb1l C T 1: 75,185,347 (GRCm39) C121Y probably damaging Het
Glod4 T A 11: 76,134,397 (GRCm39) D25V probably damaging Het
Gpc5 T A 14: 115,789,651 (GRCm39) N508K possibly damaging Het
H2-M10.3 C T 17: 36,678,722 (GRCm39) probably null Het
Ibsp C A 5: 104,453,863 (GRCm39) S67* probably null Het
Ifit1bl2 C T 19: 34,607,164 (GRCm39) probably benign Het
Iqgap2 A G 13: 95,800,569 (GRCm39) probably null Het
Kcnma1 A G 14: 23,387,097 (GRCm39) S982P probably damaging Het
Kif5b T C 18: 6,213,272 (GRCm39) S707G probably benign Het
Lrrk2 A G 15: 91,589,323 (GRCm39) I437V probably benign Het
Mapkbp1 T C 2: 119,855,545 (GRCm39) probably benign Het
Mcu T C 10: 59,303,456 (GRCm39) Y127C probably damaging Het
Mlst8 T C 17: 24,695,031 (GRCm39) Y284C probably damaging Het
Nr2f2 T G 7: 70,007,870 (GRCm39) N204T probably benign Het
Or13a26 A G 7: 140,285,004 (GRCm39) Y280C probably damaging Het
Or7a42 T A 10: 78,791,877 (GRCm39) Y279* probably null Het
Pabir3 G A X: 52,382,376 (GRCm39) R94H possibly damaging Het
Pcf11 T C 7: 92,295,696 (GRCm39) Y1451C probably damaging Het
Plekhn1 T C 4: 156,309,988 (GRCm39) S109G probably damaging Het
Rttn T C 18: 89,047,097 (GRCm39) S920P probably damaging Het
Tas2r135 A T 6: 42,383,013 (GRCm39) H184L probably benign Het
Tmem38a C T 8: 73,326,005 (GRCm39) P20S possibly damaging Het
Ubqlnl C T 7: 103,798,925 (GRCm39) V191M probably benign Het
Vmn2r102 A G 17: 19,901,475 (GRCm39) Y534C probably damaging Het
Wdr62 A G 7: 29,969,683 (GRCm39) V259A probably damaging Het
Whrn A G 4: 63,379,517 (GRCm39) probably null Het
Other mutations in Rufy4
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01617:Rufy4 APN 1 74,168,513 (GRCm39) missense probably damaging 1.00
IGL02075:Rufy4 APN 1 74,168,518 (GRCm39) missense probably damaging 1.00
IGL02604:Rufy4 APN 1 74,173,348 (GRCm39) missense probably damaging 1.00
IGL02606:Rufy4 APN 1 74,172,509 (GRCm39) splice site probably benign
IGL02928:Rufy4 APN 1 74,168,241 (GRCm39) unclassified probably benign
R0091:Rufy4 UTSW 1 74,168,095 (GRCm39) unclassified probably benign
R0507:Rufy4 UTSW 1 74,185,875 (GRCm39) missense probably benign 0.02
R0589:Rufy4 UTSW 1 74,172,042 (GRCm39) missense probably damaging 1.00
R0595:Rufy4 UTSW 1 74,180,089 (GRCm39) missense possibly damaging 0.94
R0742:Rufy4 UTSW 1 74,185,875 (GRCm39) missense probably benign 0.02
R1533:Rufy4 UTSW 1 74,169,002 (GRCm39) critical splice donor site probably null
R1666:Rufy4 UTSW 1 74,186,837 (GRCm39) missense probably benign 0.06
R1668:Rufy4 UTSW 1 74,186,837 (GRCm39) missense probably benign 0.06
R1827:Rufy4 UTSW 1 74,173,279 (GRCm39) missense probably damaging 1.00
R2018:Rufy4 UTSW 1 74,180,106 (GRCm39) missense possibly damaging 0.49
R2095:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R2306:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R2307:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R2472:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R2475:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3022:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3054:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3055:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3056:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3117:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3118:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3236:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3237:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3545:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3546:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3547:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3548:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3767:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3768:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3770:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3816:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3817:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3818:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3819:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3895:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4050:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4091:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4117:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4124:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4125:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4127:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4231:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4233:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4234:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4236:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4254:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4255:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4319:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4320:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4321:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4321:Rufy4 UTSW 1 74,171,943 (GRCm39) missense possibly damaging 0.93
R4322:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4323:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4324:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4360:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4361:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4406:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4408:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4516:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4520:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4522:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4524:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4531:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4533:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4617:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4661:Rufy4 UTSW 1 74,172,266 (GRCm39) missense probably damaging 0.99
R4778:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4779:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4840:Rufy4 UTSW 1 74,168,198 (GRCm39) missense possibly damaging 0.82
R4897:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4898:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4899:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4915:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4917:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4918:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4997:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5092:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5097:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5189:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5191:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5195:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5196:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5197:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5226:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5227:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5228:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5230:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5372:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5373:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5374:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5375:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5376:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5377:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5378:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5699:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5748:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5750:Rufy4 UTSW 1 74,172,068 (GRCm39) missense probably benign 0.01
R5767:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5865:Rufy4 UTSW 1 74,185,914 (GRCm39) missense probably damaging 0.99
R6083:Rufy4 UTSW 1 74,168,556 (GRCm39) missense probably damaging 0.99
R6149:Rufy4 UTSW 1 74,186,892 (GRCm39) missense probably benign 0.15
R6279:Rufy4 UTSW 1 74,172,383 (GRCm39) missense probably benign 0.00
R6300:Rufy4 UTSW 1 74,172,383 (GRCm39) missense probably benign 0.00
R6629:Rufy4 UTSW 1 74,171,526 (GRCm39) splice site probably null
R6809:Rufy4 UTSW 1 74,172,206 (GRCm39) missense probably benign 0.00
R7179:Rufy4 UTSW 1 74,172,035 (GRCm39) missense probably benign 0.12
R7218:Rufy4 UTSW 1 74,172,174 (GRCm39) missense probably damaging 0.99
R7453:Rufy4 UTSW 1 74,168,493 (GRCm39) splice site probably null
R9377:Rufy4 UTSW 1 74,171,879 (GRCm39) missense probably benign 0.08
X0023:Rufy4 UTSW 1 74,180,208 (GRCm39) missense probably benign 0.04
X0025:Rufy4 UTSW 1 74,172,178 (GRCm39) missense probably benign
Predicted Primers PCR Primer
(F):5'- AAGCCATCAGGAGAAGCTGC -3'
(R):5'- AGCTAGGCCTATCTGTGGAC -3'

Sequencing Primer
(F):5'- GGAGAAGCTGCCACCTCAATTAG -3'
(R):5'- TGGACTGCCAGCTGTTCC -3'
Posted On 2015-08-18