Mutagenetix > Incidental Mutation

Incidental Mutation 'R4530:Vcan'

333090

Institutional Source

Beutler Lab

Gene Symbol

Vcan

Ensembl Gene

ENSMUSG00000021614

Gene Name

versican

Synonyms

PG-M, hdf, heart defect, Cspg2, DPEAAE, 5430420N07Rik

MMRRC Submission

041770-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4530 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

89803431-89890628 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 89852147 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Isoleucine at position 938 (F938I)

Ref Sequence

ENSEMBL: ENSMUSP00000105173 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109543] [ENSMUST00000109544] [ENSMUST00000109546] [ENSMUST00000159910]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000109543

SMART Domains

Protein: ENSMUSP00000105170
Gene: ENSMUSG00000021614

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
EGF	351	384	2.72e-7	SMART
EGF_CA	386	422	1.16e-10	SMART
CLECT	428	549	3.08e-34	SMART
CCP	555	611	1.04e-8	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000109544
AA Change: F938I

PolyPhen 2 Score 0.880 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000105171
Gene: ENSMUSG00000021614
AA Change: F938I

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	728	743	N/A	INTRINSIC
low complexity region	1205	1219	N/A	INTRINSIC
EGF	1311	1344	2.72e-7	SMART
EGF_CA	1346	1382	1.16e-10	SMART
CLECT	1388	1509	3.08e-34	SMART
CCP	1515	1571	1.04e-8	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000109546
AA Change: F938I

PolyPhen 2 Score 0.974 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000105173
Gene: ENSMUSG00000021614
AA Change: F938I

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	728	743	N/A	INTRINSIC
low complexity region	1205	1219	N/A	INTRINSIC
low complexity region	1322	1333	N/A	INTRINSIC
low complexity region	1546	1569	N/A	INTRINSIC
low complexity region	1837	1852	N/A	INTRINSIC
low complexity region	2013	2026	N/A	INTRINSIC
low complexity region	2354	2367	N/A	INTRINSIC
low complexity region	2468	2482	N/A	INTRINSIC
low complexity region	2719	2728	N/A	INTRINSIC
EGF	3050	3083	2.72e-7	SMART
EGF_CA	3085	3121	1.16e-10	SMART
CLECT	3127	3248	3.08e-34	SMART
CCP	3254	3310	1.04e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000159910

SMART Domains

Protein: ENSMUSP00000125446
Gene: ENSMUSG00000021614

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	362	373	N/A	INTRINSIC
low complexity region	586	609	N/A	INTRINSIC
low complexity region	877	892	N/A	INTRINSIC
low complexity region	1053	1066	N/A	INTRINSIC
low complexity region	1394	1407	N/A	INTRINSIC
low complexity region	1508	1522	N/A	INTRINSIC
low complexity region	1759	1768	N/A	INTRINSIC
EGF	2090	2123	2.72e-7	SMART
EGF_CA	2125	2161	1.16e-10	SMART
CLECT	2167	2288	3.08e-34	SMART
CCP	2294	2350	1.04e-8	SMART

Meta Mutation Damage Score

0.0938

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.6%

Validation Efficiency

93% (37/40)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
PHENOTYPE: Homozygotes for an insertional mutation exhibit anterior-posterior segmental defects of the heart, lack endocardial cushions of the conus and atrioventricular region, and die and around embryonic day 10.5. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 36 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adcy4	T	C	14: 56,016,485 (GRCm39)	D322G	probably damaging	Het
Akap9	T	A	5: 4,093,948 (GRCm39)	F2157I	probably damaging	Het
Arhgap42	T	C	9: 9,011,433 (GRCm39)	D451G	probably damaging	Het
Arhgef7	T	C	8: 11,850,802 (GRCm39)	M144T	possibly damaging	Het
Arid4b	C	A	13: 14,301,040 (GRCm39)	T41N	probably damaging	Het
Axin1	T	A	17: 26,407,146 (GRCm39)	Y580N	probably benign	Het
Cdc27	T	C	11: 104,419,252 (GRCm39)	N227D	possibly damaging	Het
Cetn4	C	T	3: 37,364,094 (GRCm39)	V39I	probably benign	Het
Clec2h	A	G	6: 128,639,457 (GRCm39)	D18G	possibly damaging	Het
Clec4e	T	C	6: 123,266,733 (GRCm39)		probably benign	Het
Cntnap4	T	C	8: 113,584,842 (GRCm39)	I1093T	probably benign	Het
Dner	G	T	1: 84,560,736 (GRCm39)	N136K	probably damaging	Het
Gpr158	A	G	2: 21,373,811 (GRCm39)	S249G	probably benign	Het
Il16	A	C	7: 83,330,518 (GRCm39)		probably benign	Het
Intu	T	G	3: 40,637,794 (GRCm39)	C427G	possibly damaging	Het
Kif21a	A	C	15: 90,852,292 (GRCm39)		probably null	Het
Lin54	G	A	5: 100,594,419 (GRCm39)	T582I	possibly damaging	Het
Mroh7	T	C	4: 106,577,634 (GRCm39)	E348G	possibly damaging	Het
Or10g6	G	A	9: 39,934,589 (GRCm39)	R300K	probably benign	Het
Or2h1b	C	T	17: 37,462,498 (GRCm39)	V122M	possibly damaging	Het
Or8g2b	G	T	9: 39,751,379 (GRCm39)	M216I	probably benign	Het
Or8k38	T	C	2: 86,487,905 (GRCm39)	D299G	probably benign	Het
Pabir3	G	A	X: 52,382,376 (GRCm39)	R94H	possibly damaging	Het
Plbd1	T	A	6: 136,628,823 (GRCm39)	I82F	probably benign	Het
Prss43	A	T	9: 110,658,572 (GRCm39)	M291L	probably benign	Het
Rap1gds1	T	C	3: 138,663,186 (GRCm39)	N338D	probably damaging	Het
Rsf1	CGGCGGCGG	CGGCGGCGGGGGCGGCGG	7: 97,229,130 (GRCm39)		probably benign	Het
Stip1	G	T	19: 7,013,026 (GRCm39)	N19K	probably benign	Het
Tat	T	A	8: 110,722,842 (GRCm39)	F301L	probably benign	Het
Tmprss11a	C	A	5: 86,576,540 (GRCm39)	V104L	possibly damaging	Het
Ttc3	T	A	16: 94,267,736 (GRCm39)		probably benign	Het
Tubgcp3	G	T	8: 12,713,932 (GRCm39)	L62I	probably damaging	Het
Wrap73	A	G	4: 154,241,164 (GRCm39)		probably benign	Het
Xndc1	C	A	7: 101,727,942 (GRCm39)	N85K	probably benign	Het
Zfp282	C	T	6: 47,867,567 (GRCm39)	P248S	probably benign	Het
Zfp930	C	T	8: 69,681,483 (GRCm39)	Q393*	probably null	Het

Other mutations in Vcan

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00434:Vcan	APN	13	89,852,821 (GRCm39)	missense	probably damaging	1.00
IGL00502:Vcan	APN	13	89,840,438 (GRCm39)	missense	probably benign
IGL00504:Vcan	APN	13	89,839,394 (GRCm39)	missense	possibly damaging	0.70
IGL00566:Vcan	APN	13	89,837,098 (GRCm39)	missense	probably benign	0.01
IGL00701:Vcan	APN	13	89,851,845 (GRCm39)	missense	probably benign
IGL00743:Vcan	APN	13	89,873,425 (GRCm39)	missense	probably damaging	0.98
IGL00962:Vcan	APN	13	89,810,171 (GRCm39)	missense	probably damaging	1.00
IGL01085:Vcan	APN	13	89,828,077 (GRCm39)	missense	probably damaging	1.00
IGL01317:Vcan	APN	13	89,839,787 (GRCm39)	missense	probably benign	0.00
IGL01349:Vcan	APN	13	89,852,062 (GRCm39)	missense	probably damaging	0.98
IGL01391:Vcan	APN	13	89,852,288 (GRCm39)	missense	probably benign	0.19
IGL01644:Vcan	APN	13	89,836,794 (GRCm39)	missense	probably benign	0.13
IGL01657:Vcan	APN	13	89,838,705 (GRCm39)	missense	probably damaging	1.00
IGL01707:Vcan	APN	13	89,837,864 (GRCm39)	missense	probably damaging	1.00
IGL01764:Vcan	APN	13	89,873,507 (GRCm39)	missense	probably damaging	1.00
IGL01920:Vcan	APN	13	89,837,324 (GRCm39)	missense	probably benign	0.04
IGL01989:Vcan	APN	13	89,837,478 (GRCm39)	missense	possibly damaging	0.86
IGL01999:Vcan	APN	13	89,832,557 (GRCm39)	missense	probably damaging	1.00
IGL02083:Vcan	APN	13	89,873,684 (GRCm39)	missense	probably damaging	1.00
IGL02160:Vcan	APN	13	89,832,612 (GRCm39)	missense	probably damaging	1.00
IGL02217:Vcan	APN	13	89,851,196 (GRCm39)	missense	probably damaging	1.00
IGL02522:Vcan	APN	13	89,852,968 (GRCm39)	missense	probably benign	0.00
IGL02527:Vcan	APN	13	89,838,776 (GRCm39)	missense	possibly damaging	0.95
IGL02926:Vcan	APN	13	89,836,742 (GRCm39)	missense	probably damaging	0.98
IGL03061:Vcan	APN	13	89,851,394 (GRCm39)	missense	probably benign	0.25
IGL03331:Vcan	APN	13	89,810,051 (GRCm39)	missense	probably damaging	1.00
IGL03352:Vcan	APN	13	89,853,125 (GRCm39)	missense	probably benign	0.00
R0041:Vcan	UTSW	13	89,810,104 (GRCm39)	missense	probably damaging	1.00
R0102:Vcan	UTSW	13	89,851,787 (GRCm39)	missense	probably benign	0.01
R0102:Vcan	UTSW	13	89,851,787 (GRCm39)	missense	probably benign	0.01
R0109:Vcan	UTSW	13	89,826,192 (GRCm39)	critical splice donor site	probably null
R0139:Vcan	UTSW	13	89,839,380 (GRCm39)	missense	probably damaging	1.00
R0295:Vcan	UTSW	13	89,860,310 (GRCm39)	missense	probably benign	0.06
R0375:Vcan	UTSW	13	89,839,394 (GRCm39)	missense	probably damaging	0.99
R0379:Vcan	UTSW	13	89,851,665 (GRCm39)	missense	probably damaging	0.99
R0457:Vcan	UTSW	13	89,851,318 (GRCm39)	missense	possibly damaging	0.78
R0482:Vcan	UTSW	13	89,826,264 (GRCm39)	missense	probably damaging	1.00
R0485:Vcan	UTSW	13	89,852,779 (GRCm39)	missense	possibly damaging	0.92
R0532:Vcan	UTSW	13	89,851,891 (GRCm39)	missense	probably damaging	0.99
R0561:Vcan	UTSW	13	89,879,583 (GRCm39)	missense	possibly damaging	0.86
R0561:Vcan	UTSW	13	89,860,372 (GRCm39)	missense	probably damaging	1.00
R0636:Vcan	UTSW	13	89,860,386 (GRCm39)	missense	probably damaging	1.00
R0636:Vcan	UTSW	13	89,852,825 (GRCm39)	missense	probably damaging	0.99
R0680:Vcan	UTSW	13	89,827,941 (GRCm39)	missense	probably damaging	1.00
R0849:Vcan	UTSW	13	89,853,072 (GRCm39)	missense	possibly damaging	0.75
R1006:Vcan	UTSW	13	89,833,196 (GRCm39)	critical splice donor site	probably null
R1104:Vcan	UTSW	13	89,840,529 (GRCm39)	missense	probably damaging	1.00
R1118:Vcan	UTSW	13	89,853,782 (GRCm39)	missense	probably damaging	1.00
R1137:Vcan	UTSW	13	89,852,422 (GRCm39)	missense	probably damaging	1.00
R1199:Vcan	UTSW	13	89,827,913 (GRCm39)	splice site	probably null
R1219:Vcan	UTSW	13	89,828,023 (GRCm39)	missense	probably damaging	1.00
R1296:Vcan	UTSW	13	89,805,675 (GRCm39)	missense	probably damaging	1.00
R1332:Vcan	UTSW	13	89,841,174 (GRCm39)	missense	probably damaging	1.00
R1336:Vcan	UTSW	13	89,841,174 (GRCm39)	missense	probably damaging	1.00
R1403:Vcan	UTSW	13	89,836,603 (GRCm39)	missense	probably benign	0.00
R1403:Vcan	UTSW	13	89,836,603 (GRCm39)	missense	probably benign	0.00
R1546:Vcan	UTSW	13	89,841,075 (GRCm39)	missense	probably damaging	0.99
R1604:Vcan	UTSW	13	89,837,780 (GRCm39)	missense	probably benign	0.42
R1616:Vcan	UTSW	13	89,853,782 (GRCm39)	missense	probably damaging	1.00
R1636:Vcan	UTSW	13	89,851,786 (GRCm39)	missense	possibly damaging	0.90
R1654:Vcan	UTSW	13	89,810,065 (GRCm39)	missense	probably damaging	1.00
R1680:Vcan	UTSW	13	89,851,666 (GRCm39)	missense	probably benign	0.19
R1694:Vcan	UTSW	13	89,836,602 (GRCm39)	missense	probably damaging	0.98
R1712:Vcan	UTSW	13	89,869,894 (GRCm39)	missense	probably damaging	1.00
R1754:Vcan	UTSW	13	89,852,854 (GRCm39)	missense	probably benign	0.01
R1756:Vcan	UTSW	13	89,839,800 (GRCm39)	missense	probably benign	0.05
R1824:Vcan	UTSW	13	89,853,331 (GRCm39)	missense	possibly damaging	0.75
R1852:Vcan	UTSW	13	89,853,511 (GRCm39)	missense	probably damaging	0.99
R1868:Vcan	UTSW	13	89,838,990 (GRCm39)	missense	probably benign	0.12
R1920:Vcan	UTSW	13	89,841,134 (GRCm39)	missense	probably damaging	1.00
R1932:Vcan	UTSW	13	89,853,653 (GRCm39)	missense	possibly damaging	0.78
R1934:Vcan	UTSW	13	89,851,045 (GRCm39)	missense	probably damaging	1.00
R1942:Vcan	UTSW	13	89,851,543 (GRCm39)	missense	probably benign	0.01
R1964:Vcan	UTSW	13	89,840,861 (GRCm39)	missense	probably benign	0.02
R1970:Vcan	UTSW	13	89,837,157 (GRCm39)	missense	probably damaging	1.00
R2045:Vcan	UTSW	13	89,839,104 (GRCm39)	missense	probably benign	0.00
R2110:Vcan	UTSW	13	89,841,422 (GRCm39)	missense	probably damaging	1.00
R2111:Vcan	UTSW	13	89,841,422 (GRCm39)	missense	probably damaging	1.00
R2112:Vcan	UTSW	13	89,841,422 (GRCm39)	missense	probably damaging	1.00
R2136:Vcan	UTSW	13	89,837,856 (GRCm39)	missense	probably damaging	1.00
R2158:Vcan	UTSW	13	89,851,648 (GRCm39)	missense	possibly damaging	0.68
R2376:Vcan	UTSW	13	89,851,529 (GRCm39)	missense	possibly damaging	0.80
R2385:Vcan	UTSW	13	89,837,568 (GRCm39)	missense	probably damaging	1.00
R2443:Vcan	UTSW	13	89,852,794 (GRCm39)	missense	probably damaging	1.00
R2876:Vcan	UTSW	13	89,852,356 (GRCm39)	missense	probably damaging	1.00
R3607:Vcan	UTSW	13	89,851,420 (GRCm39)	missense	probably damaging	0.98
R4042:Vcan	UTSW	13	89,840,662 (GRCm39)	missense	probably benign	0.35
R4043:Vcan	UTSW	13	89,840,662 (GRCm39)	missense	probably benign	0.35
R4044:Vcan	UTSW	13	89,840,662 (GRCm39)	missense	probably benign	0.35
R4065:Vcan	UTSW	13	89,828,006 (GRCm39)	missense	probably damaging	1.00
R4161:Vcan	UTSW	13	89,833,277 (GRCm39)	missense	probably damaging	1.00
R4178:Vcan	UTSW	13	89,873,666 (GRCm39)	missense	probably damaging	1.00
R4290:Vcan	UTSW	13	89,873,605 (GRCm39)	missense	probably damaging	1.00
R4666:Vcan	UTSW	13	89,828,053 (GRCm39)	missense	probably damaging	1.00
R4785:Vcan	UTSW	13	89,853,908 (GRCm39)	missense	probably damaging	1.00
R4870:Vcan	UTSW	13	89,852,858 (GRCm39)	missense	probably benign	0.01
R4973:Vcan	UTSW	13	89,836,961 (GRCm39)	missense	probably benign	0.30
R5037:Vcan	UTSW	13	89,852,096 (GRCm39)	missense	probably damaging	1.00
R5104:Vcan	UTSW	13	89,805,591 (GRCm39)	intron	probably benign
R5124:Vcan	UTSW	13	89,873,636 (GRCm39)	missense	probably damaging	1.00
R5129:Vcan	UTSW	13	89,838,359 (GRCm39)	missense	probably damaging	1.00
R5198:Vcan	UTSW	13	89,838,991 (GRCm39)	missense	probably damaging	1.00
R5240:Vcan	UTSW	13	89,840,651 (GRCm39)	missense	probably benign	0.08
R5254:Vcan	UTSW	13	89,839,719 (GRCm39)	missense	probably damaging	0.99
R5280:Vcan	UTSW	13	89,838,405 (GRCm39)	missense	probably benign	0.00
R5522:Vcan	UTSW	13	89,839,929 (GRCm39)	missense	possibly damaging	0.62
R5557:Vcan	UTSW	13	89,851,231 (GRCm39)	missense	possibly damaging	0.77
R5568:Vcan	UTSW	13	89,836,790 (GRCm39)	missense	probably damaging	1.00
R5578:Vcan	UTSW	13	89,839,622 (GRCm39)	missense	probably benign	0.01
R5627:Vcan	UTSW	13	89,839,254 (GRCm39)	frame shift	probably null
R5687:Vcan	UTSW	13	89,826,253 (GRCm39)	missense	probably damaging	1.00
R5752:Vcan	UTSW	13	89,828,069 (GRCm39)	missense	probably damaging	1.00
R5879:Vcan	UTSW	13	89,852,071 (GRCm39)	missense	probably damaging	0.99
R5941:Vcan	UTSW	13	89,840,810 (GRCm39)	missense	probably damaging	0.98
R6113:Vcan	UTSW	13	89,805,655 (GRCm39)	nonsense	probably null
R6135:Vcan	UTSW	13	89,838,045 (GRCm39)	missense	probably benign	0.36
R6252:Vcan	UTSW	13	89,839,339 (GRCm39)	nonsense	probably null
R6280:Vcan	UTSW	13	89,873,492 (GRCm39)	missense	probably damaging	1.00
R6317:Vcan	UTSW	13	89,839,716 (GRCm39)	missense	probably benign	0.22
R6327:Vcan	UTSW	13	89,852,951 (GRCm39)	missense	probably damaging	0.99
R6460:Vcan	UTSW	13	89,838,806 (GRCm39)	missense	possibly damaging	0.61
R6669:Vcan	UTSW	13	89,852,850 (GRCm39)	missense	probably benign	0.21
R6744:Vcan	UTSW	13	89,853,301 (GRCm39)	missense	probably damaging	1.00
R6819:Vcan	UTSW	13	89,853,244 (GRCm39)	missense	probably benign	0.00
R6880:Vcan	UTSW	13	89,860,500 (GRCm39)	missense	probably damaging	1.00
R6956:Vcan	UTSW	13	89,837,550 (GRCm39)	missense	probably damaging	0.99
R6971:Vcan	UTSW	13	89,826,252 (GRCm39)	missense	probably damaging	1.00
R6985:Vcan	UTSW	13	89,828,075 (GRCm39)	missense	probably damaging	1.00
R6994:Vcan	UTSW	13	89,841,526 (GRCm39)	missense	possibly damaging	0.94
R6997:Vcan	UTSW	13	89,838,737 (GRCm39)	missense	probably damaging	0.98
R7029:Vcan	UTSW	13	89,838,360 (GRCm39)	missense	probably damaging	1.00
R7066:Vcan	UTSW	13	89,853,805 (GRCm39)	missense	probably damaging	1.00
R7156:Vcan	UTSW	13	89,837,229 (GRCm39)	missense	possibly damaging	0.95
R7171:Vcan	UTSW	13	89,873,710 (GRCm39)	missense	probably damaging	1.00
R7176:Vcan	UTSW	13	89,837,055 (GRCm39)	missense	probably benign	0.01
R7229:Vcan	UTSW	13	89,853,389 (GRCm39)	missense	possibly damaging	0.87
R7250:Vcan	UTSW	13	89,879,576 (GRCm39)	critical splice donor site	probably null
R7250:Vcan	UTSW	13	89,869,805 (GRCm39)	missense	probably damaging	1.00
R7262:Vcan	UTSW	13	89,853,280 (GRCm39)	missense	possibly damaging	0.62
R7289:Vcan	UTSW	13	89,840,852 (GRCm39)	nonsense	probably null
R7299:Vcan	UTSW	13	89,853,385 (GRCm39)	missense	probably benign
R7301:Vcan	UTSW	13	89,853,385 (GRCm39)	missense	probably benign
R7425:Vcan	UTSW	13	89,837,951 (GRCm39)	missense	probably damaging	0.99
R7514:Vcan	UTSW	13	89,852,237 (GRCm39)	missense	probably damaging	0.97
R7579:Vcan	UTSW	13	89,840,577 (GRCm39)	missense	probably damaging	1.00
R7618:Vcan	UTSW	13	89,840,342 (GRCm39)	missense	probably damaging	0.99
R7655:Vcan	UTSW	13	89,833,233 (GRCm39)	missense	probably damaging	1.00
R7656:Vcan	UTSW	13	89,833,233 (GRCm39)	missense	probably damaging	1.00
R7676:Vcan	UTSW	13	89,839,908 (GRCm39)	missense	probably damaging	1.00
R7719:Vcan	UTSW	13	89,852,738 (GRCm39)	missense	probably damaging	0.98
R7753:Vcan	UTSW	13	89,837,442 (GRCm39)	missense	probably damaging	1.00
R7762:Vcan	UTSW	13	89,841,056 (GRCm39)	missense	probably damaging	1.00
R7778:Vcan	UTSW	13	89,836,773 (GRCm39)	missense	probably damaging	1.00
R7824:Vcan	UTSW	13	89,836,773 (GRCm39)	missense	probably damaging	1.00
R7995:Vcan	UTSW	13	89,839,977 (GRCm39)	missense	probably benign
R7998:Vcan	UTSW	13	89,852,446 (GRCm39)	missense	probably damaging	1.00
R8033:Vcan	UTSW	13	89,852,479 (GRCm39)	missense	probably benign	0.04
R8061:Vcan	UTSW	13	89,805,409 (GRCm39)	missense	probably benign	0.45
R8103:Vcan	UTSW	13	89,851,439 (GRCm39)	nonsense	probably null
R8103:Vcan	UTSW	13	89,805,777 (GRCm39)	missense	probably damaging	1.00
R8124:Vcan	UTSW	13	89,852,373 (GRCm39)	missense	possibly damaging	0.93
R8162:Vcan	UTSW	13	89,853,106 (GRCm39)	nonsense	probably null
R8166:Vcan	UTSW	13	89,840,855 (GRCm39)	missense	probably benign	0.02
R8274:Vcan	UTSW	13	89,853,089 (GRCm39)	missense	probably benign	0.02
R8284:Vcan	UTSW	13	89,852,454 (GRCm39)	missense	possibly damaging	0.68
R8417:Vcan	UTSW	13	89,836,862 (GRCm39)	missense	probably benign	0.19
R8696:Vcan	UTSW	13	89,839,217 (GRCm39)	missense	probably benign	0.00
R8738:Vcan	UTSW	13	89,840,439 (GRCm39)	missense	probably benign	0.17
R8792:Vcan	UTSW	13	89,840,230 (GRCm39)	missense	possibly damaging	0.91
R8887:Vcan	UTSW	13	89,853,026 (GRCm39)	missense	probably benign
R9049:Vcan	UTSW	13	89,826,224 (GRCm39)	missense	probably damaging	1.00
R9074:Vcan	UTSW	13	89,839,146 (GRCm39)	missense	possibly damaging	0.95
R9095:Vcan	UTSW	13	89,852,644 (GRCm39)	missense	probably benign	0.32
R9172:Vcan	UTSW	13	89,828,050 (GRCm39)	missense	probably damaging	1.00
R9199:Vcan	UTSW	13	89,838,615 (GRCm39)	nonsense	probably null
R9259:Vcan	UTSW	13	89,838,989 (GRCm39)	missense	probably damaging	0.99
R9455:Vcan	UTSW	13	89,837,452 (GRCm39)	missense	probably damaging	1.00
R9476:Vcan	UTSW	13	89,851,531 (GRCm39)	missense	possibly damaging	0.95
R9477:Vcan	UTSW	13	89,841,128 (GRCm39)	missense	probably damaging	1.00
R9555:Vcan	UTSW	13	89,839,659 (GRCm39)	missense
R9579:Vcan	UTSW	13	89,837,713 (GRCm39)	missense	possibly damaging	0.67
R9606:Vcan	UTSW	13	89,853,491 (GRCm39)	missense	probably damaging	1.00
R9645:Vcan	UTSW	13	89,841,081 (GRCm39)	missense	probably benign	0.00
R9659:Vcan	UTSW	13	89,839,860 (GRCm39)	missense	probably damaging	0.99
R9766:Vcan	UTSW	13	89,839,247 (GRCm39)	missense	probably benign	0.00
R9778:Vcan	UTSW	13	89,837,930 (GRCm39)	missense	probably damaging	1.00
X0058:Vcan	UTSW	13	89,840,612 (GRCm39)	missense	probably benign	0.21
X0065:Vcan	UTSW	13	89,853,868 (GRCm39)	missense	probably damaging	0.96
Z1176:Vcan	UTSW	13	89,840,690 (GRCm39)	missense	probably benign	0.10
Z1177:Vcan	UTSW	13	89,852,192 (GRCm39)	missense	probably damaging	1.00
Z1177:Vcan	UTSW	13	89,851,907 (GRCm39)	nonsense	probably null
Z1177:Vcan	UTSW	13	89,851,643 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- AAAGCTCCAGGAGACATGGTTG -3'
(R):5'- CATCTACATCAATTGGCAGTGC -3'

Sequencing Primer
(F):5'- CTCCAGGAGACATGGTTGCTTCTAG -3'
(R):5'- CAGTGCAGAAAAGTCAGCTTC -3'

Posted On

2015-08-18