|Institutional Source||Beutler Lab|
|Gene Name||serine/arginine-rich splicing factor 4|
|Synonyms||MNCb-2616, Sfrs4, SRp75, 5730499P16Rik|
|Is this an essential gene?||Possibly essential (E-score: 0.514)|
|Stock #||R4535 (G1)|
|Chromosomal Location||131873617-131901706 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to G at 131873864 bp (GRCm38)|
|Amino Acid Change||Lysine to Arginine at position 34 (K34R)|
|Ref Sequence||ENSEMBL: ENSMUSP00000061474 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000053819]|
|AlphaFold||no structure available at present|
AA Change: K34R
PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
AA Change: K34R
AA Change: K16R
|Coding Region Coverage||
|MGI Phenotype||FUNCTION: The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. [provided by RefSeq, Sep 2010]|
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Srsf4||
(F):5'- AGGACGTGCTGCAGCTGT -3'
(R):5'- CATAGTCCAGCGACGGAAA -3'
(F):5'- TGCAGCTGTCGCTCACTCG -3'
(R):5'- AGTCTAACCCGGGCCTC -3'