Mutagenetix > Incidental Mutation

Incidental Mutation 'R4535:Vmn2r70'

333290

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r70

Ensembl Gene

ENSMUSG00000090806

Gene Name

vomeronasal 2, receptor 70

Synonyms

EG620835

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.130) question?

Stock #

R4535 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

85207911-85218296 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 85214541 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tryptophan to Arginine at position 204 (W204R)

Ref Sequence

ENSEMBL: ENSMUSP00000129703 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168230]

AlphaFold

K7N702

Predicted Effect

probably damaging
Transcript: ENSMUST00000168230
AA Change: W204R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000129703
Gene: ENSMUSG00000090806
AA Change: W204R

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:ANF_receptor	77	468	2.5e-28	PFAM
Pfam:NCD3G	510	562	1.5e-19	PFAM
Pfam:7tm_3	592	830	1.2e-52	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Arhgef4	T	C	1: 34,762,162 (GRCm39)	S473P	unknown	Het
Azin1	A	T	15: 38,493,849 (GRCm39)	I258N	probably benign	Het
Bod1l	G	A	5: 41,989,574 (GRCm39)	A383V	probably benign	Het
Carmil3	GGACGA	GGA	14: 55,736,933 (GRCm39)		probably benign	Het
Cd200r3	T	A	16: 44,774,552 (GRCm39)	D188E	probably benign	Het
Cd4	A	T	6: 124,847,414 (GRCm39)	F250Y	probably benign	Het
Clcn4	T	A	7: 7,290,813 (GRCm39)	Y662F	probably benign	Het
Cpa2	T	C	6: 30,552,020 (GRCm39)	V249A	probably benign	Het
Depdc5	CTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCT	CTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCT	5: 33,067,751 (GRCm39)		probably benign	Het
Dhx57	A	G	17: 80,582,511 (GRCm39)	Y365H	probably damaging	Het
Dsg1c	A	G	18: 20,408,322 (GRCm39)	E457G	probably benign	Het
Eef2k	T	A	7: 120,457,822 (GRCm39)	Y60*	probably null	Het
Efcc1	A	G	6: 87,730,133 (GRCm39)	D482G	probably null	Het
Exoc4	C	T	6: 33,254,179 (GRCm39)	R112C	probably damaging	Het
Fam178b	A	T	1: 36,639,606 (GRCm39)	D293E	probably benign	Het
Fbxl21	G	A	13: 56,674,873 (GRCm39)	V49I	probably damaging	Het
Fyco1	A	G	9: 123,667,953 (GRCm39)	V91A	probably damaging	Het
H2-M10.4	T	C	17: 36,772,736 (GRCm39)	E82G	probably damaging	Het
Hmcn1	A	G	1: 150,439,531 (GRCm39)	I5434T	probably damaging	Het
Hormad1	T	C	3: 95,492,452 (GRCm39)	V343A	probably benign	Het
Incenp	T	C	19: 9,861,303 (GRCm39)	N450S	unknown	Het
Iqsec3	T	C	6: 121,356,977 (GRCm39)	K1035E	possibly damaging	Het
Ltn1	A	T	16: 87,223,174 (GRCm39)	V102D	probably damaging	Het
Mcur1	T	C	13: 43,698,016 (GRCm39)	T295A	probably damaging	Het
Pals1	A	T	12: 78,871,611 (GRCm39)	D397V	possibly damaging	Het
Pcdha3	T	C	18: 37,081,013 (GRCm39)	V585A	probably damaging	Het
Plcd4	A	G	1: 74,602,627 (GRCm39)	T594A	probably damaging	Het
Ppp1r3c	T	C	19: 36,711,522 (GRCm39)	K83E	probably damaging	Het
Sesn3	C	A	9: 14,233,954 (GRCm39)	T309K	probably benign	Het
Slc38a3	T	C	9: 107,533,405 (GRCm39)	N251S	probably benign	Het
Sptbn4	A	G	7: 27,067,127 (GRCm39)	V614A	probably damaging	Het
Srsf4	A	G	4: 131,601,175 (GRCm39)	K34R	probably damaging	Het
Tfpi2	T	C	6: 3,968,044 (GRCm39)	N32S	possibly damaging	Het
Ttll2	A	T	17: 7,619,120 (GRCm39)	I269N	probably benign	Het
Utp3	T	C	5: 88,703,458 (GRCm39)	V329A	probably benign	Het
Vmn2r102	A	G	17: 19,914,975 (GRCm39)	T847A	probably benign	Het
Xrcc3	A	G	12: 111,770,966 (GRCm39)	L321P	probably damaging	Het

Other mutations in Vmn2r70

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00978:Vmn2r70	APN	7	85,213,007 (GRCm39)	missense	probably benign	0.00
IGL01140:Vmn2r70	APN	7	85,214,379 (GRCm39)	nonsense	probably null
IGL01287:Vmn2r70	APN	7	85,218,227 (GRCm39)	nonsense	probably null
IGL01581:Vmn2r70	APN	7	85,213,122 (GRCm39)	splice site	probably null
IGL01632:Vmn2r70	APN	7	85,215,280 (GRCm39)	missense	probably benign	0.00
IGL01725:Vmn2r70	APN	7	85,208,594 (GRCm39)	missense	probably damaging	1.00
IGL02244:Vmn2r70	APN	7	85,214,211 (GRCm39)	missense	probably benign
IGL02288:Vmn2r70	APN	7	85,214,342 (GRCm39)	missense	probably benign	0.31
IGL02313:Vmn2r70	APN	7	85,214,376 (GRCm39)	missense	probably damaging	0.99
IGL02591:Vmn2r70	APN	7	85,214,153 (GRCm39)	missense	probably damaging	0.96
IGL02725:Vmn2r70	APN	7	85,214,553 (GRCm39)	missense	possibly damaging	0.46
IGL02797:Vmn2r70	APN	7	85,208,295 (GRCm39)	missense	probably benign	0.00
R0045:Vmn2r70	UTSW	7	85,215,252 (GRCm39)	missense	probably damaging	1.00
R0729:Vmn2r70	UTSW	7	85,215,112 (GRCm39)	missense	probably benign	0.00
R0967:Vmn2r70	UTSW	7	85,208,827 (GRCm39)	missense	probably damaging	0.99
R1217:Vmn2r70	UTSW	7	85,208,269 (GRCm39)	missense	probably damaging	1.00
R1351:Vmn2r70	UTSW	7	85,214,262 (GRCm39)	missense	probably damaging	1.00
R1387:Vmn2r70	UTSW	7	85,207,969 (GRCm39)	missense	probably benign	0.12
R1483:Vmn2r70	UTSW	7	85,208,375 (GRCm39)	missense	probably benign	0.04
R1796:Vmn2r70	UTSW	7	85,213,011 (GRCm39)	nonsense	probably null
R1809:Vmn2r70	UTSW	7	85,215,130 (GRCm39)	missense	probably benign	0.23
R2154:Vmn2r70	UTSW	7	85,212,923 (GRCm39)	missense	possibly damaging	0.67
R2173:Vmn2r70	UTSW	7	85,214,290 (GRCm39)	missense	probably benign
R2334:Vmn2r70	UTSW	7	85,208,800 (GRCm39)	missense	probably benign	0.05
R2871:Vmn2r70	UTSW	7	85,208,227 (GRCm39)	missense	probably damaging	1.00
R2871:Vmn2r70	UTSW	7	85,208,227 (GRCm39)	missense	probably damaging	1.00
R3975:Vmn2r70	UTSW	7	85,208,540 (GRCm39)	missense	probably benign	0.00
R4525:Vmn2r70	UTSW	7	85,208,787 (GRCm39)	missense	probably damaging	1.00
R4527:Vmn2r70	UTSW	7	85,208,787 (GRCm39)	missense	probably damaging	1.00
R5181:Vmn2r70	UTSW	7	85,208,387 (GRCm39)	missense	probably damaging	0.99
R5600:Vmn2r70	UTSW	7	85,212,935 (GRCm39)	missense	probably benign	0.07
R5641:Vmn2r70	UTSW	7	85,208,572 (GRCm39)	missense	probably damaging	0.99
R5726:Vmn2r70	UTSW	7	85,208,315 (GRCm39)	missense	probably damaging	1.00
R5943:Vmn2r70	UTSW	7	85,215,199 (GRCm39)	missense	probably benign	0.09
R6166:Vmn2r70	UTSW	7	85,215,189 (GRCm39)	missense	probably benign	0.25
R6272:Vmn2r70	UTSW	7	85,208,194 (GRCm39)	missense	probably damaging	1.00
R6324:Vmn2r70	UTSW	7	85,208,087 (GRCm39)	missense	probably benign	0.01
R6429:Vmn2r70	UTSW	7	85,208,276 (GRCm39)	missense	probably damaging	1.00
R6449:Vmn2r70	UTSW	7	85,214,157 (GRCm39)	missense	probably damaging	1.00
R6512:Vmn2r70	UTSW	7	85,215,305 (GRCm39)	missense	probably benign
R7000:Vmn2r70	UTSW	7	85,208,819 (GRCm39)	missense	probably damaging	0.99
R7141:Vmn2r70	UTSW	7	85,208,044 (GRCm39)	missense	probably benign
R7153:Vmn2r70	UTSW	7	85,214,262 (GRCm39)	missense	probably damaging	1.00
R7424:Vmn2r70	UTSW	7	85,213,076 (GRCm39)	missense	probably damaging	1.00
R7565:Vmn2r70	UTSW	7	85,214,499 (GRCm39)	missense	probably benign	0.35
R7567:Vmn2r70	UTSW	7	85,214,243 (GRCm39)	missense	probably benign	0.41
R7593:Vmn2r70	UTSW	7	85,215,312 (GRCm39)	nonsense	probably null
R7660:Vmn2r70	UTSW	7	85,218,130 (GRCm39)	missense	probably damaging	0.99
R7806:Vmn2r70	UTSW	7	85,208,401 (GRCm39)	missense	probably benign
R7892:Vmn2r70	UTSW	7	85,208,588 (GRCm39)	missense	possibly damaging	0.58
R7965:Vmn2r70	UTSW	7	85,211,071 (GRCm39)	missense	probably damaging	0.96
R8052:Vmn2r70	UTSW	7	85,212,923 (GRCm39)	missense	probably benign
R8251:Vmn2r70	UTSW	7	85,215,186 (GRCm39)	nonsense	probably null
R8814:Vmn2r70	UTSW	7	85,215,169 (GRCm39)	missense	probably benign	0.00
R8934:Vmn2r70	UTSW	7	85,211,188 (GRCm39)	missense	possibly damaging	0.87
R9225:Vmn2r70	UTSW	7	85,208,242 (GRCm39)	missense	probably damaging	1.00
R9322:Vmn2r70	UTSW	7	85,208,498 (GRCm39)	missense	possibly damaging	0.92
R9430:Vmn2r70	UTSW	7	85,215,240 (GRCm39)	missense	probably benign	0.10
R9477:Vmn2r70	UTSW	7	85,218,244 (GRCm39)	missense	possibly damaging	0.50
Z1088:Vmn2r70	UTSW	7	85,213,968 (GRCm39)	missense	possibly damaging	0.53
Z1176:Vmn2r70	UTSW	7	85,218,253 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- AGATTATGGGAGAGTCTTTGTATCC -3'
(R):5'- CTCGCATGAATATACTTTGCTCAAG -3'

Sequencing Primer
(F):5'- TGGGAGAGTCTTTGTATCCATAAAC -3'
(R):5'- GCTCAAGCAAAATCTGATCTTACAAG -3'

Posted On

2015-08-18