Mutagenetix > Incidental Mutation

Incidental Mutation 'R4545:Zfp810'

333722

Institutional Source

Beutler Lab

Gene Symbol

Zfp810

Ensembl Gene

ENSMUSG00000066829

Gene Name

zinc finger protein 810

Synonyms

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.088) question?

Stock #

R4545 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

22276748-22307648 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 22278745 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Isoleucine at position 289 (T289I)

Ref Sequence

ENSEMBL: ENSMUSP00000083459 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086278] [ENSMUST00000215202]

AlphaFold

Q99K45

Predicted Effect

probably damaging
Transcript: ENSMUST00000086278
AA Change: T289I

PolyPhen 2 Score 0.965 (Sensitivity: 0.78; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000083459
Gene: ENSMUSG00000066829
AA Change: T289I

Domain	Start	End	E-Value	Type
KRAB	4	64	1.09e-33	SMART
ZnF_C2H2	126	148	2.44e2	SMART
ZnF_C2H2	182	204	3.07e-1	SMART
ZnF_C2H2	210	232	8.47e-4	SMART
ZnF_C2H2	238	260	6.78e-3	SMART
ZnF_C2H2	266	288	6.13e-1	SMART
ZnF_C2H2	294	316	5.06e-2	SMART
ZnF_C2H2	322	344	4.79e-3	SMART
ZnF_C2H2	350	372	2.99e-4	SMART
ZnF_C2H2	378	400	1.33e-1	SMART
ZnF_C2H2	406	428	2.75e-3	SMART
ZnF_C2H2	434	456	1.58e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000214499

Predicted Effect

probably benign
Transcript: ENSMUST00000215202

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.1%
20x: 94.7%

Validation Efficiency

98% (41/42)

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Akr1c20	A	C	13: 4,507,844	V201G	probably damaging	Het
Atf7	A	G	15: 102,534,327	V449A	probably benign	Het
Ccr1	G	A	9: 123,964,400	A31V	probably benign	Het
Chrna6	A	T	8: 27,406,683	S389T	probably benign	Het
Clic6	C	T	16: 92,492,157		probably benign	Het
Cmya5	G	A	13: 93,091,918	R2221*	probably null	Het
Coq8b	G	T	7: 27,233,505	C13F	probably benign	Het
Cspg4	T	A	9: 56,888,629	L1216Q	possibly damaging	Het
Decr1	C	A	4: 15,930,979	V118F	probably damaging	Het
Dlec1	A	T	9: 119,128,078	I796F	probably damaging	Het
Dnajc11	T	A	4: 151,979,941	D516E	probably damaging	Het
Dock8	G	A	19: 25,188,358	V1869M	probably damaging	Het
Dst	A	G	1: 34,188,738	D1982G	probably damaging	Het
Gm11808	C	T	4: 3,973,244	R106H	probably benign	Het
Gnptab	G	A	10: 88,414,595	D190N	probably benign	Het
Golga4	A	G	9: 118,556,845	K22E	probably damaging	Het
Hecw2	T	C	1: 53,813,222	*1579W	probably null	Het
Ica1l	A	G	1: 60,013,818		probably null	Het
Ift122	T	A	6: 115,890,588	L433Q	probably damaging	Het
Iqgap1	T	C	7: 80,762,567		probably null	Het
Klra13-ps	T	C	6: 130,291,269		noncoding transcript	Het
Mndal	A	T	1: 173,875,664	Y58*	probably null	Het
Mvb12b	G	C	2: 33,827,700	P172R	possibly damaging	Het
Ncapg	T	C	5: 45,671,212	F102L	probably damaging	Het
Olfr1447	T	A	19: 12,901,268	K171*	probably null	Het
Olfr235	T	C	19: 12,268,824	V198A	possibly damaging	Het
Olfr390	T	C	11: 73,787,166	V76A	probably damaging	Het
Pde8a	A	T	7: 81,328,099	R713S	probably damaging	Het
Rbks	T	C	5: 31,624,568	N296S	probably benign	Het
Sema3c	G	A	5: 17,694,772	V421I	probably benign	Het
Tm9sf1	A	G	14: 55,638,108	V393A	possibly damaging	Het
Tubgcp2	G	A	7: 139,996,071	P893L	possibly damaging	Het
Vnn3	G	A	10: 23,856,326	R158H	probably benign	Het
Zfa-ps	G	T	10: 52,544,936		noncoding transcript	Het
Zfp414	T	C	17: 33,631,648		probably benign	Het
Zfp819	G	T	7: 43,617,785	R488L	probably damaging	Het
Zfp942	C	T	17: 21,928,304	G448D	probably benign	Het
Zscan12	A	G	13: 21,366,705	K165E	possibly damaging	Het

Other mutations in Zfp810

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00484:Zfp810	APN	9	22278309	nonsense	probably null
IGL03079:Zfp810	APN	9	22284127	missense	probably damaging	1.00
IGL03402:Zfp810	APN	9	22279145	splice site	probably null
H8562:Zfp810	UTSW	9	22279091	missense	probably benign	0.42
R1116:Zfp810	UTSW	9	22279085	missense	probably benign	0.11
R1160:Zfp810	UTSW	9	22278532	missense	possibly damaging	0.64
R1171:Zfp810	UTSW	9	22278826	missense	possibly damaging	0.95
R1393:Zfp810	UTSW	9	22280514	missense	probably benign
R1608:Zfp810	UTSW	9	22278920	missense	probably benign	0.00
R1644:Zfp810	UTSW	9	22279028	missense	possibly damaging	0.67
R1766:Zfp810	UTSW	9	22278532	missense	possibly damaging	0.64
R2568:Zfp810	UTSW	9	22279238	missense	probably benign	0.01
R3684:Zfp810	UTSW	9	22278235	missense	probably benign	0.01
R4002:Zfp810	UTSW	9	22278892	missense	probably damaging	1.00
R4134:Zfp810	UTSW	9	22279073	missense	probably damaging	0.97
R4135:Zfp810	UTSW	9	22279073	missense	probably damaging	0.97
R4334:Zfp810	UTSW	9	22278784	missense	probably benign	0.00
R5399:Zfp810	UTSW	9	22278829	missense	possibly damaging	0.91
R5622:Zfp810	UTSW	9	22279096	missense	probably benign	0.00
R5643:Zfp810	UTSW	9	22283171	missense	probably benign	0.26
R7375:Zfp810	UTSW	9	22290537	critical splice donor site	probably null
R7441:Zfp810	UTSW	9	22279272	nonsense	probably null
R7809:Zfp810	UTSW	9	22278982	missense	possibly damaging	0.51
R8422:Zfp810	UTSW	9	22283222	nonsense	probably null
R8526:Zfp810	UTSW	9	22278290	missense	probably damaging	1.00
R8719:Zfp810	UTSW	9	22279275	missense	probably benign	0.00
R9177:Zfp810	UTSW	9	22278640	missense	probably damaging	1.00
R9479:Zfp810	UTSW	9	22283201	missense	possibly damaging	0.68
R9521:Zfp810	UTSW	9	22278931	missense	possibly damaging	0.81
R9683:Zfp810	UTSW	9	22278503	missense	possibly damaging	0.90

Predicted Primers

PCR Primer
(F):5'- AACGGACTTATTAAGTATTACCCTGCT -3'
(R):5'- TGCAAGTCTCACCTCACTGT -3'

Sequencing Primer
(F):5'- ACTCGGGACATTCATATGGC -3'
(R):5'- TCTGAGAATTCACACCGGTG -3'

Posted On

2015-08-18