Mutagenetix > Incidental Mutation

Incidental Mutation 'R4520:Gm5444'

334191

Institutional Source

Beutler Lab

Gene Symbol

Gm5444

Ensembl Gene

ENSMUSG00000053499

Gene Name

predicted gene 5444

Synonyms

MMRRC Submission

041763-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.080) question?

Stock #

R4520 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

4821650-4886484 bp(+) (GRCm39)

Type of Mutation

exon

DNA Base Change (assembly)

A to G at 4884225 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s):

AlphaFold

no structure available at present

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000065956

SMART Domains

Protein: ENSMUSP00000070493
Gene: ENSMUSG00000053499

Domain	Start	End	E-Value	Type
transmembrane domain	7	29	N/A	INTRINSIC
low complexity region	83	103	N/A	INTRINSIC

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	A	G	3: 137,776,027 (GRCm39)	T1739A	probably damaging	Het
Ascc3	A	G	10: 50,536,766 (GRCm39)	N700D	probably benign	Het
Ccdc88c	G	T	12: 100,879,591 (GRCm39)	S1843R	possibly damaging	Het
Cel	C	T	2: 28,447,980 (GRCm39)	V349M	probably benign	Het
Cspp1	A	G	1: 10,204,452 (GRCm39)	T1127A	probably benign	Het
Cyp2b10	T	C	7: 25,610,982 (GRCm39)	V113A	probably benign	Het
Cyp2c50	A	G	19: 40,079,133 (GRCm39)	T159A	probably benign	Het
Degs1	T	C	1: 182,104,373 (GRCm39)	D304G	possibly damaging	Het
Esr2	G	A	12: 76,214,323 (GRCm39)	P43S	possibly damaging	Het
Fbxo41	A	G	6: 85,461,024 (GRCm39)	I228T	probably damaging	Het
Golga3	T	A	5: 110,351,617 (GRCm39)	L790*	probably null	Het
Gtpbp6	T	C	5: 110,255,725 (GRCm39)	T105A	probably benign	Het
Ido2	T	C	8: 25,066,194 (GRCm39)	E24G	probably damaging	Het
Ihh	T	A	1: 74,990,109 (GRCm39)	I89F	probably damaging	Het
Krt13	A	G	11: 100,010,174 (GRCm39)	M269T	probably damaging	Het
Marf1	T	C	16: 13,950,530 (GRCm39)	H952R	probably damaging	Het
Mga	T	A	2: 119,778,579 (GRCm39)	F2041L	possibly damaging	Het
Msmo1	A	C	8: 65,173,557 (GRCm39)		probably benign	Het
Mterf1a	A	G	5: 3,940,992 (GRCm39)	V292A	probably damaging	Het
Naxe	A	G	3: 87,965,289 (GRCm39)		probably null	Het
Ntng1	A	G	3: 109,842,312 (GRCm39)	S154P	probably damaging	Het
Or51h5	A	T	7: 102,577,764 (GRCm39)	I310F	probably benign	Het
Or56b1	T	A	7: 104,285,376 (GRCm39)	V165E	probably damaging	Het
Or8h7	T	C	2: 86,721,363 (GRCm39)	D52G	probably benign	Het
Otog	T	A	7: 45,890,477 (GRCm39)		probably benign	Het
Pigo	A	T	4: 43,020,301 (GRCm39)	H880Q	probably benign	Het
Plce1	T	C	19: 38,512,763 (GRCm39)	S21P	possibly damaging	Het
Pole	T	A	5: 110,445,790 (GRCm39)	D555E	probably damaging	Het
Prkag2	A	G	5: 25,071,169 (GRCm39)	V403A	probably damaging	Het
Psg28	T	A	7: 18,156,826 (GRCm39)	M470L	probably benign	Het
Rpgrip1	A	G	14: 52,389,746 (GRCm39)	T1138A	probably benign	Het
Rpl10l	T	C	12: 66,330,512 (GRCm39)	D207G	probably benign	Het
Rufy4	T	C	1: 74,186,822 (GRCm39)	C537R	probably damaging	Het
Serpinb9c	T	C	13: 33,335,840 (GRCm39)		probably null	Het
Sh2d6	T	C	6: 72,495,936 (GRCm39)	N101D	possibly damaging	Het
Shisa8	C	G	15: 82,096,163 (GRCm39)	V151L	possibly damaging	Het
Slc13a1	T	C	6: 24,134,512 (GRCm39)	T124A	probably benign	Het
Slc6a3	G	A	13: 73,688,975 (GRCm39)	V100M	possibly damaging	Het
Sspn	A	G	6: 145,907,033 (GRCm39)	T79A	probably benign	Het
Tlr8	C	T	X: 166,026,171 (GRCm39)	R893H	probably damaging	Het
Tnrc18	T	C	5: 142,717,905 (GRCm39)	K2183R	unknown	Het
Trim15	T	C	17: 37,177,242 (GRCm39)	I139M	probably benign	Het
Trmt13	A	T	3: 116,375,262 (GRCm39)		probably null	Het
Vmn2r84	T	A	10: 130,222,391 (GRCm39)	T610S	probably damaging	Het
Zcchc14	A	T	8: 122,335,834 (GRCm39)		probably benign	Het

Other mutations in Gm5444

Allele	Source	Chr	Coord	Type	Predicted Effect
R0716:Gm5444	UTSW	13	4,884,192 (GRCm39)	exon	noncoding transcript
R2332:Gm5444	UTSW	13	4,883,624 (GRCm39)	exon	noncoding transcript
R2341:Gm5444	UTSW	13	4,884,344 (GRCm39)	exon	noncoding transcript
R3901:Gm5444	UTSW	13	4,884,278 (GRCm39)	exon	noncoding transcript
R5148:Gm5444	UTSW	13	4,884,314 (GRCm39)	exon	noncoding transcript
R5856:Gm5444	UTSW	13	4,821,683 (GRCm39)	exon	noncoding transcript

Predicted Primers

PCR Primer
(F):5'- CACTAGCTTCAGCCCATAGG -3'
(R):5'- TGAAGACCTGAATTCAACCACTTC -3'

Sequencing Primer
(F):5'- ATGGTTGTAAGCCACCATGC -3'
(R):5'- GCTTATAACCCTTGTGCCAGAAAG -3'

Posted On

2015-08-18