Mutagenetix > Incidental Mutation

Incidental Mutation 'R4521:Mrgprx1'

334222

Institutional Source

Beutler Lab

Gene Symbol

Mrgprx1

Ensembl Gene

ENSMUSG00000070552

Gene Name

MAS-related GPR, member X1

Synonyms

Mrgprc11, MrgC11

MMRRC Submission

041764-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4521 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

47670719-47677345 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 47671447 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 100 (D100G)

Ref Sequence

ENSEMBL: ENSMUSP00000091954 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094390]

AlphaFold

Q8CIP3

Predicted Effect

probably benign
Transcript: ENSMUST00000094390
AA Change: D100G

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000091954
Gene: ENSMUSG00000070552
AA Change: D100G

Domain	Start	End	E-Value	Type
Pfam:7tm_1	43	202	1.9e-7	PFAM
low complexity region	227	245	N/A	INTRINSIC

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.8%

Validation Efficiency

100% (49/49)

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ascc3	A	G	10: 50,536,766 (GRCm39)	N700D	probably benign	Het
Bora	G	T	14: 99,305,984 (GRCm39)	S451I	probably damaging	Het
Cadm3	A	T	1: 173,172,630 (GRCm39)		probably null	Het
Car14	A	G	3: 95,811,690 (GRCm39)		probably benign	Het
Ccdc88c	G	T	12: 100,879,591 (GRCm39)	S1843R	possibly damaging	Het
Cdc20b	A	G	13: 113,217,725 (GRCm39)	I381M	probably damaging	Het
Col12a1	C	T	9: 79,540,639 (GRCm39)	V2449I	probably benign	Het
Crb2	T	C	2: 37,685,349 (GRCm39)		probably benign	Het
Dcaf6	A	T	1: 165,218,059 (GRCm39)	D347E	probably damaging	Het
Dlgap2	G	A	8: 14,777,871 (GRCm39)	R372H	probably damaging	Het
Fat3	T	C	9: 15,834,238 (GRCm39)	N4118S	probably null	Het
Fbxo41	A	G	6: 85,461,024 (GRCm39)	I228T	probably damaging	Het
Fpr2	A	C	17: 18,113,509 (GRCm39)	R168S	probably benign	Het
Ghr	A	G	15: 3,355,440 (GRCm39)	I281T	probably damaging	Het
Glmp	C	A	3: 88,235,346 (GRCm39)	N259K	possibly damaging	Het
Grhl3	T	C	4: 135,273,561 (GRCm39)	K564E	probably damaging	Het
Helz2	T	C	2: 180,870,626 (GRCm39)	H2875R	probably benign	Het
Lcp1	A	G	14: 75,452,608 (GRCm39)	D438G	possibly damaging	Het
Lrfn2	T	A	17: 49,376,922 (GRCm39)	M1K	probably null	Het
Lrp6	A	G	6: 134,462,825 (GRCm39)	C612R	probably damaging	Het
Map3k13	T	C	16: 21,724,525 (GRCm39)	V341A	possibly damaging	Het
Megf8	T	A	7: 25,042,126 (GRCm39)	C1315S	probably benign	Het
Mrgpra9	A	T	7: 46,884,938 (GRCm39)	L243H	probably damaging	Het
Nop2	G	T	6: 125,110,515 (GRCm39)	R47L	probably damaging	Het
Nup93	T	C	8: 95,041,264 (GRCm39)	Y801H	probably damaging	Het
Or51b6b	C	T	7: 103,309,539 (GRCm39)	R306H	probably benign	Het
Or6c69b	T	A	10: 129,627,050 (GRCm39)	N136I	possibly damaging	Het
Orc4	G	A	2: 48,827,501 (GRCm39)	P31S	probably benign	Het
Plce1	T	C	19: 38,512,763 (GRCm39)	S21P	possibly damaging	Het
Plpp2	A	G	10: 79,366,459 (GRCm39)	Y118H	probably damaging	Het
Ppwd1	A	G	13: 104,346,167 (GRCm39)	V496A	probably benign	Het
Rbm20	A	G	19: 53,805,633 (GRCm39)	N466S	probably benign	Het
Rpl10l	T	C	12: 66,330,512 (GRCm39)	D207G	probably benign	Het
Skida1	T	C	2: 18,050,683 (GRCm39)		probably benign	Het
Stk17b	A	G	1: 53,803,197 (GRCm39)	Y73H	probably damaging	Het
Tapbpl	A	G	6: 125,205,085 (GRCm39)	I287T	probably damaging	Het
Tmem38a	C	T	8: 73,326,005 (GRCm39)	P20S	possibly damaging	Het
Topbp1	T	C	9: 103,211,401 (GRCm39)		probably benign	Het
Tsen54	G	A	11: 115,707,932 (GRCm39)		probably null	Het
Ttn	G	A	2: 76,555,560 (GRCm39)	R28736*	probably null	Het
Wdr36	G	A	18: 32,974,201 (GRCm39)		probably null	Het

Other mutations in Mrgprx1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01152:Mrgprx1	APN	7	47,671,234 (GRCm39)	missense	probably benign	0.00
IGL01326:Mrgprx1	APN	7	47,671,517 (GRCm39)	missense	probably benign	0.26
IGL02117:Mrgprx1	APN	7	47,671,371 (GRCm39)	nonsense	probably null
IGL02219:Mrgprx1	APN	7	47,671,477 (GRCm39)	missense	probably benign	0.20
IGL02431:Mrgprx1	APN	7	47,670,875 (GRCm39)	missense	probably benign	0.00
IGL02441:Mrgprx1	APN	7	47,671,336 (GRCm39)	missense	probably benign	0.39
IGL02682:Mrgprx1	APN	7	47,671,740 (GRCm39)	missense	probably damaging	1.00
R0219:Mrgprx1	UTSW	7	47,671,294 (GRCm39)	missense	probably damaging	1.00
R4366:Mrgprx1	UTSW	7	47,670,941 (GRCm39)	missense	probably damaging	0.98
R4801:Mrgprx1	UTSW	7	47,670,959 (GRCm39)	missense	possibly damaging	0.89
R4802:Mrgprx1	UTSW	7	47,670,959 (GRCm39)	missense	possibly damaging	0.89
R5452:Mrgprx1	UTSW	7	47,671,556 (GRCm39)	missense	probably benign	0.07
R5537:Mrgprx1	UTSW	7	47,670,898 (GRCm39)	missense	probably benign
R6444:Mrgprx1	UTSW	7	47,671,562 (GRCm39)	missense	possibly damaging	0.87
R6834:Mrgprx1	UTSW	7	47,671,385 (GRCm39)	missense	probably damaging	0.99
R7406:Mrgprx1	UTSW	7	47,671,733 (GRCm39)	missense	possibly damaging	0.62
R9664:Mrgprx1	UTSW	7	47,671,273 (GRCm39)	missense	probably benign	0.38
RF020:Mrgprx1	UTSW	7	47,671,259 (GRCm39)	small insertion	probably benign
RF024:Mrgprx1	UTSW	7	47,671,259 (GRCm39)	small insertion	probably benign
RF026:Mrgprx1	UTSW	7	47,671,257 (GRCm39)	small insertion	probably benign
RF043:Mrgprx1	UTSW	7	47,671,257 (GRCm39)	small insertion	probably benign
Z1088:Mrgprx1	UTSW	7	47,670,877 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- TGATGAGTCTCACCCAGGAATCC -3'
(R):5'- TTGGACTGGCAGGAAACACC -3'

Sequencing Primer
(F):5'- TCCTGAGAACCAATCGAGGATGC -3'
(R):5'- GGAAACACCATTGTACTCTGGCTC -3'

Posted On

2015-08-18