|Institutional Source||Beutler Lab|
|Gene Name||dual specificity phosphatase 5|
|Is this an essential gene?||Non essential (E-score: 0.000)|
|Stock #||R4523 (G1)|
|Chromosomal Location||53529109-53542431 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to G at 53537601 bp|
|Amino Acid Change||Tyrosine to Aspartic acid at position 225 (Y225D)|
|Ref Sequence||ENSEMBL: ENSMUSP00000047900 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000038287]|
|Predicted Effect||probably damaging
AA Change: Y225D
PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
AA Change: Y225D
|Meta Mutation Damage Score||0.9717|
|Coding Region Coverage||
|Validation Efficiency||100% (61/61)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, is expressed in a variety of tissues with the highest levels in pancreas and brain, and is localized in the nucleus. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit increased proliferation and apoptosis and altered metabolic profiles in T cells. Mice homozygous for other null alleles exhibit altered eosinophilic response to parasicitc infection and increased susceptibility to induced tumors. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Dusp5||
(F):5'- TCTTCTTCCGAATGAGCAAGC -3'
(R):5'- GGTTCCTTCCTTTCCAAGAAGG -3'
(F):5'- GAGCAAGCTAATCGGATCTTTCCTG -3'
(R):5'- CCTTCCTTTCCAAGAAGGTAACC -3'