Mutagenetix > Incidental Mutation

Incidental Mutation 'R4526:Vmn2r60'

334444

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r60

Ensembl Gene

ENSMUSG00000090619

Gene Name

vomeronasal 2, receptor 60

Synonyms

Casr-rs3, EG637898, Gprc2a-rs3

MMRRC Submission

041591-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.100) question?

Stock #

R4526 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

41765895-41845200 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 41844667 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 677 (T677A)

Ref Sequence

ENSEMBL: ENSMUSP00000128493 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166447]

AlphaFold

A0A3B2WBC8

Predicted Effect

probably damaging
Transcript: ENSMUST00000166447
AA Change: T677A

PolyPhen 2 Score 0.960 (Sensitivity: 0.78; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000128493
Gene: ENSMUSG00000090619
AA Change: T677A

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	78	471	1.2e-44	PFAM
Pfam:NCD3G	514	567	5.1e-23	PFAM
Pfam:7tm_3	600	835	1.4e-51	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930474N05Rik	C	T	14: 35,818,535 (GRCm39)	R178C	probably damaging	Het
Adamts16	G	A	13: 70,927,637 (GRCm39)		probably benign	Het
Adgre4	C	A	17: 56,092,016 (GRCm39)	S92*	probably null	Het
Arl13b	T	C	16: 62,632,374 (GRCm39)	D73G	probably damaging	Het
Cd109	CATTTATTTATTTATTTATTTATTTATTTATTTAT	CATTTATTTATTTATTTATTTATTTATTTATTTATTTAT	9: 78,619,782 (GRCm39)		probably benign	Het
Crebzf	A	C	7: 90,092,968 (GRCm39)	E16A	possibly damaging	Het
D430041D05Rik	A	G	2: 104,022,778 (GRCm39)		probably null	Het
Dnah1	C	A	14: 31,007,955 (GRCm39)	D2151Y	probably benign	Het
Gli3	A	G	13: 15,888,216 (GRCm39)	K544E	probably damaging	Het
Gm17542	T	C	10: 58,549,435 (GRCm39)	D31G	probably null	Het
Lcn9	A	C	2: 25,714,520 (GRCm39)	K128T	possibly damaging	Het
Lman2l	T	C	1: 36,477,844 (GRCm39)	E164G	probably damaging	Het
Lpin2	T	A	17: 71,544,373 (GRCm39)		probably null	Het
Ly75	G	A	2: 60,161,117 (GRCm39)	T900M	probably benign	Het
Mapk15	A	T	15: 75,867,104 (GRCm39)	I74F	possibly damaging	Het
Myh10	C	T	11: 68,705,875 (GRCm39)	T2007I	probably benign	Het
Nek1	T	C	8: 61,559,978 (GRCm39)	S937P	probably damaging	Het
Oas1b	T	A	5: 120,960,167 (GRCm39)		probably null	Het
Or4x6	T	C	2: 89,949,016 (GRCm39)	K309E	probably benign	Het
Or5l13	T	C	2: 87,779,753 (GRCm39)	T275A	probably benign	Het
Or8h8	C	T	2: 86,753,339 (GRCm39)	C179Y	possibly damaging	Het
Otogl	G	A	10: 107,722,841 (GRCm39)	P297S	probably damaging	Het
Plekhm1	A	G	11: 103,286,130 (GRCm39)	S102P	probably damaging	Het
Plvap	G	A	8: 71,960,415 (GRCm39)	R334W	probably damaging	Het
Polr2b	T	G	5: 77,474,561 (GRCm39)	V466G	probably damaging	Het
Ptpn13	T	C	5: 103,649,335 (GRCm39)	I246T	probably benign	Het
Rims2	A	T	15: 39,301,113 (GRCm39)	K281N	probably damaging	Het
Scamp4	T	C	10: 80,446,891 (GRCm39)	F108S	probably damaging	Het
Snrnp200	A	G	2: 127,071,022 (GRCm39)	N1101S	probably benign	Het
Sntb2	C	A	8: 107,736,595 (GRCm39)	L490M	probably damaging	Het
Ssu2	A	G	6: 112,359,383 (GRCm39)	V79A	possibly damaging	Het
Stard7	A	G	2: 127,139,128 (GRCm39)	S347G	probably benign	Het
Usp5	C	G	6: 124,799,593 (GRCm39)	K318N	possibly damaging	Het
Vmn2r6	A	G	3: 64,445,145 (GRCm39)	V860A	probably benign	Het
Zfp318	T	A	17: 46,723,284 (GRCm39)	H1762Q	probably benign	Het
Zmynd8	TTGCTGCTGCTGCTGCTG	TTGCTGCTGCTGCTG	2: 165,649,527 (GRCm39)		probably benign	Het
Zng1	A	G	19: 24,935,328 (GRCm39)	Y59H	probably benign	Het

Other mutations in Vmn2r60

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01622:Vmn2r60	APN	7	41,785,910 (GRCm39)	missense	probably benign	0.09
IGL01623:Vmn2r60	APN	7	41,785,910 (GRCm39)	missense	probably benign	0.09
IGL02363:Vmn2r60	APN	7	41,844,578 (GRCm39)	missense	probably benign	0.02
IGL02485:Vmn2r60	APN	7	41,844,890 (GRCm39)	missense	possibly damaging	0.54
IGL02651:Vmn2r60	APN	7	41,845,010 (GRCm39)	missense	probably damaging	0.99
IGL02660:Vmn2r60	APN	7	41,791,720 (GRCm39)	nonsense	probably null
IGL03135:Vmn2r60	APN	7	41,786,018 (GRCm39)	missense	probably benign	0.13
IGL03307:Vmn2r60	APN	7	41,765,971 (GRCm39)	missense	probably benign	0.14
R0310:Vmn2r60	UTSW	7	41,844,564 (GRCm39)	missense	possibly damaging	0.54
R0314:Vmn2r60	UTSW	7	41,784,985 (GRCm39)	splice site	probably benign
R0328:Vmn2r60	UTSW	7	41,791,744 (GRCm39)	splice site	probably benign
R0464:Vmn2r60	UTSW	7	41,785,255 (GRCm39)	missense	probably damaging	0.99
R0755:Vmn2r60	UTSW	7	41,844,869 (GRCm39)	missense	probably damaging	1.00
R1119:Vmn2r60	UTSW	7	41,844,365 (GRCm39)	missense	possibly damaging	0.68
R1162:Vmn2r60	UTSW	7	41,845,195 (GRCm39)	missense	probably benign	0.29
R1241:Vmn2r60	UTSW	7	41,786,476 (GRCm39)	missense	probably benign	0.01
R1404:Vmn2r60	UTSW	7	41,786,211 (GRCm39)	missense	probably damaging	0.99
R1404:Vmn2r60	UTSW	7	41,786,211 (GRCm39)	missense	probably damaging	0.99
R1488:Vmn2r60	UTSW	7	41,786,137 (GRCm39)	missense	probably benign	0.17
R1623:Vmn2r60	UTSW	7	41,785,279 (GRCm39)	nonsense	probably null
R1628:Vmn2r60	UTSW	7	41,785,830 (GRCm39)	nonsense	probably null
R1883:Vmn2r60	UTSW	7	41,786,094 (GRCm39)	missense	probably damaging	0.99
R1884:Vmn2r60	UTSW	7	41,786,094 (GRCm39)	missense	probably damaging	0.99
R2182:Vmn2r60	UTSW	7	41,844,931 (GRCm39)	missense	probably benign	0.06
R2275:Vmn2r60	UTSW	7	41,786,251 (GRCm39)	nonsense	probably null
R2847:Vmn2r60	UTSW	7	41,785,857 (GRCm39)	missense	probably benign	0.07
R2885:Vmn2r60	UTSW	7	41,790,403 (GRCm39)	missense	possibly damaging	0.91
R2894:Vmn2r60	UTSW	7	41,785,220 (GRCm39)	missense	probably benign
R2921:Vmn2r60	UTSW	7	41,790,459 (GRCm39)	missense	probably damaging	0.98
R2922:Vmn2r60	UTSW	7	41,790,459 (GRCm39)	missense	probably damaging	0.98
R3772:Vmn2r60	UTSW	7	41,765,980 (GRCm39)	missense	probably benign	0.35
R3820:Vmn2r60	UTSW	7	41,785,125 (GRCm39)	missense	probably damaging	0.98
R3822:Vmn2r60	UTSW	7	41,785,125 (GRCm39)	missense	probably damaging	0.98
R3872:Vmn2r60	UTSW	7	41,785,878 (GRCm39)	missense	probably benign	0.19
R4222:Vmn2r60	UTSW	7	41,765,952 (GRCm39)	missense	probably benign	0.08
R4223:Vmn2r60	UTSW	7	41,765,952 (GRCm39)	missense	probably benign	0.08
R4224:Vmn2r60	UTSW	7	41,765,952 (GRCm39)	missense	probably benign	0.08
R4547:Vmn2r60	UTSW	7	41,785,087 (GRCm39)	missense	probably null	0.54
R4840:Vmn2r60	UTSW	7	41,785,285 (GRCm39)	missense	probably damaging	1.00
R5173:Vmn2r60	UTSW	7	41,844,935 (GRCm39)	missense	probably damaging	0.97
R5231:Vmn2r60	UTSW	7	41,786,448 (GRCm39)	missense	possibly damaging	0.93
R5480:Vmn2r60	UTSW	7	41,785,154 (GRCm39)	missense	probably damaging	0.98
R5521:Vmn2r60	UTSW	7	41,845,049 (GRCm39)	missense	probably damaging	0.99
R5834:Vmn2r60	UTSW	7	41,765,932 (GRCm39)	missense	probably benign	0.17
R6038:Vmn2r60	UTSW	7	41,844,386 (GRCm39)	missense	probably benign	0.04
R6038:Vmn2r60	UTSW	7	41,844,386 (GRCm39)	missense	probably benign	0.04
R6112:Vmn2r60	UTSW	7	41,844,847 (GRCm39)	missense	probably damaging	1.00
R6149:Vmn2r60	UTSW	7	41,786,400 (GRCm39)	missense	probably damaging	1.00
R6170:Vmn2r60	UTSW	7	41,785,045 (GRCm39)	missense	possibly damaging	0.94
R6383:Vmn2r60	UTSW	7	41,765,895 (GRCm39)	start codon destroyed	probably null	0.04
R6811:Vmn2r60	UTSW	7	41,844,310 (GRCm39)	missense	probably damaging	1.00
R6876:Vmn2r60	UTSW	7	41,785,087 (GRCm39)	missense	probably null	0.54
R6997:Vmn2r60	UTSW	7	41,791,716 (GRCm39)	missense	probably benign	0.00
R7040:Vmn2r60	UTSW	7	41,791,666 (GRCm39)	missense	probably benign	0.00
R7116:Vmn2r60	UTSW	7	41,786,487 (GRCm39)	missense	probably benign	0.00
R7128:Vmn2r60	UTSW	7	41,844,536 (GRCm39)	missense	probably damaging	0.96
R7232:Vmn2r60	UTSW	7	41,786,166 (GRCm39)	missense	possibly damaging	0.83
R7296:Vmn2r60	UTSW	7	41,785,826 (GRCm39)	missense	probably benign	0.01
R7376:Vmn2r60	UTSW	7	41,844,631 (GRCm39)	missense	probably damaging	1.00
R7526:Vmn2r60	UTSW	7	41,845,158 (GRCm39)	frame shift	probably null
R7527:Vmn2r60	UTSW	7	41,845,158 (GRCm39)	frame shift	probably null
R7528:Vmn2r60	UTSW	7	41,845,158 (GRCm39)	frame shift	probably null
R7764:Vmn2r60	UTSW	7	41,844,535 (GRCm39)	missense	probably damaging	0.99
R7843:Vmn2r60	UTSW	7	41,844,511 (GRCm39)	missense	probably benign	0.00
R8080:Vmn2r60	UTSW	7	41,790,521 (GRCm39)	missense	probably benign	0.30
R8290:Vmn2r60	UTSW	7	41,791,690 (GRCm39)	missense	probably damaging	1.00
R8342:Vmn2r60	UTSW	7	41,790,494 (GRCm39)	missense	possibly damaging	0.63
R8362:Vmn2r60	UTSW	7	41,844,954 (GRCm39)	missense	probably damaging	1.00
R8418:Vmn2r60	UTSW	7	41,844,850 (GRCm39)	missense	probably damaging	0.97
R8848:Vmn2r60	UTSW	7	41,786,169 (GRCm39)	missense	probably damaging	1.00
R8860:Vmn2r60	UTSW	7	41,791,654 (GRCm39)	missense	probably damaging	0.99
R8882:Vmn2r60	UTSW	7	41,790,518 (GRCm39)	missense	probably benign	0.00
R8913:Vmn2r60	UTSW	7	41,785,778 (GRCm39)	missense	probably benign	0.27
R9190:Vmn2r60	UTSW	7	41,844,935 (GRCm39)	missense	probably damaging	0.99
R9229:Vmn2r60	UTSW	7	41,791,723 (GRCm39)	missense	possibly damaging	0.95
R9295:Vmn2r60	UTSW	7	41,785,955 (GRCm39)	missense	probably benign	0.01
R9335:Vmn2r60	UTSW	7	41,844,332 (GRCm39)	missense	probably damaging	1.00
R9796:Vmn2r60	UTSW	7	41,785,172 (GRCm39)	missense	probably benign
RF024:Vmn2r60	UTSW	7	41,790,363 (GRCm39)	missense	probably benign	0.01
X0023:Vmn2r60	UTSW	7	41,790,538 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- AGAGACACACCCATTGTCAAGG -3'
(R):5'- CTGTTGAACCCATGTTGCACAC -3'

Sequencing Primer
(F):5'- TTGTCAAGGCCAATAACAGGACTCTC -3'
(R):5'- GTTGCACACAATGATGATGTGACC -3'

Posted On

2015-08-18