Mutagenetix > Incidental Mutation

Incidental Mutation 'R4526:Mapk15'

334458

Institutional Source

Beutler Lab

Gene Symbol

Mapk15

Ensembl Gene

ENSMUSG00000063704

Gene Name

mitogen-activated protein kinase 15

Synonyms

MMRRC Submission

041591-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4526 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

75865618-75871003 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 75867104 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 74 (I74F)

Ref Sequence

ENSEMBL: ENSMUSP00000087098 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000089669]

AlphaFold

Q80Y86

Predicted Effect

possibly damaging
Transcript: ENSMUST00000089669
AA Change: I74F

PolyPhen 2 Score 0.832 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000087098
Gene: ENSMUSG00000063704
AA Change: I74F

Domain	Start	End	E-Value	Type
S_TKc	14	305	7.08e-97	SMART
low complexity region	391	404	N/A	INTRINSIC
low complexity region	424	434	N/A	INTRINSIC
low complexity region	475	505	N/A	INTRINSIC
low complexity region	513	525	N/A	INTRINSIC
low complexity region	538	548	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000160092

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000160637

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000161961

Predicted Effect

probably benign
Transcript: ENSMUST00000230929

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930474N05Rik	C	T	14: 35,818,535 (GRCm39)	R178C	probably damaging	Het
Adamts16	G	A	13: 70,927,637 (GRCm39)		probably benign	Het
Adgre4	C	A	17: 56,092,016 (GRCm39)	S92*	probably null	Het
Arl13b	T	C	16: 62,632,374 (GRCm39)	D73G	probably damaging	Het
Cd109	CATTTATTTATTTATTTATTTATTTATTTATTTAT	CATTTATTTATTTATTTATTTATTTATTTATTTATTTAT	9: 78,619,782 (GRCm39)		probably benign	Het
Crebzf	A	C	7: 90,092,968 (GRCm39)	E16A	possibly damaging	Het
D430041D05Rik	A	G	2: 104,022,778 (GRCm39)		probably null	Het
Dnah1	C	A	14: 31,007,955 (GRCm39)	D2151Y	probably benign	Het
Gli3	A	G	13: 15,888,216 (GRCm39)	K544E	probably damaging	Het
Gm17542	T	C	10: 58,549,435 (GRCm39)	D31G	probably null	Het
Lcn9	A	C	2: 25,714,520 (GRCm39)	K128T	possibly damaging	Het
Lman2l	T	C	1: 36,477,844 (GRCm39)	E164G	probably damaging	Het
Lpin2	T	A	17: 71,544,373 (GRCm39)		probably null	Het
Ly75	G	A	2: 60,161,117 (GRCm39)	T900M	probably benign	Het
Myh10	C	T	11: 68,705,875 (GRCm39)	T2007I	probably benign	Het
Nek1	T	C	8: 61,559,978 (GRCm39)	S937P	probably damaging	Het
Oas1b	T	A	5: 120,960,167 (GRCm39)		probably null	Het
Or4x6	T	C	2: 89,949,016 (GRCm39)	K309E	probably benign	Het
Or5l13	T	C	2: 87,779,753 (GRCm39)	T275A	probably benign	Het
Or8h8	C	T	2: 86,753,339 (GRCm39)	C179Y	possibly damaging	Het
Otogl	G	A	10: 107,722,841 (GRCm39)	P297S	probably damaging	Het
Plekhm1	A	G	11: 103,286,130 (GRCm39)	S102P	probably damaging	Het
Plvap	G	A	8: 71,960,415 (GRCm39)	R334W	probably damaging	Het
Polr2b	T	G	5: 77,474,561 (GRCm39)	V466G	probably damaging	Het
Ptpn13	T	C	5: 103,649,335 (GRCm39)	I246T	probably benign	Het
Rims2	A	T	15: 39,301,113 (GRCm39)	K281N	probably damaging	Het
Scamp4	T	C	10: 80,446,891 (GRCm39)	F108S	probably damaging	Het
Snrnp200	A	G	2: 127,071,022 (GRCm39)	N1101S	probably benign	Het
Sntb2	C	A	8: 107,736,595 (GRCm39)	L490M	probably damaging	Het
Ssu2	A	G	6: 112,359,383 (GRCm39)	V79A	possibly damaging	Het
Stard7	A	G	2: 127,139,128 (GRCm39)	S347G	probably benign	Het
Usp5	C	G	6: 124,799,593 (GRCm39)	K318N	possibly damaging	Het
Vmn2r6	A	G	3: 64,445,145 (GRCm39)	V860A	probably benign	Het
Vmn2r60	A	G	7: 41,844,667 (GRCm39)	T677A	probably damaging	Het
Zfp318	T	A	17: 46,723,284 (GRCm39)	H1762Q	probably benign	Het
Zmynd8	TTGCTGCTGCTGCTGCTG	TTGCTGCTGCTGCTG	2: 165,649,527 (GRCm39)		probably benign	Het
Zng1	A	G	19: 24,935,328 (GRCm39)	Y59H	probably benign	Het

Other mutations in Mapk15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01595:Mapk15	APN	15	75,867,129 (GRCm39)	missense	probably benign	0.04
IGL02075:Mapk15	APN	15	75,866,737 (GRCm39)	missense	probably benign	0.35
IGL02395:Mapk15	APN	15	75,870,019 (GRCm39)	missense	probably benign
IGL03052:Mapk15	UTSW	15	75,865,731 (GRCm39)	missense	probably benign	0.01
R0008:Mapk15	UTSW	15	75,870,103 (GRCm39)	missense	probably benign	0.08
R0109:Mapk15	UTSW	15	75,867,926 (GRCm39)	nonsense	probably null
R0109:Mapk15	UTSW	15	75,867,926 (GRCm39)	nonsense	probably null
R1148:Mapk15	UTSW	15	75,870,004 (GRCm39)	missense	probably benign
R1148:Mapk15	UTSW	15	75,870,004 (GRCm39)	missense	probably benign
R2406:Mapk15	UTSW	15	75,870,697 (GRCm39)	missense	possibly damaging	0.75
R4572:Mapk15	UTSW	15	75,870,599 (GRCm39)	splice site	probably benign
R4613:Mapk15	UTSW	15	75,867,759 (GRCm39)	missense	probably damaging	0.98
R5861:Mapk15	UTSW	15	75,868,208 (GRCm39)	unclassified	probably benign
R6912:Mapk15	UTSW	15	75,865,747 (GRCm39)	missense	probably damaging	0.99
R7554:Mapk15	UTSW	15	75,867,745 (GRCm39)	missense	possibly damaging	0.63
R7620:Mapk15	UTSW	15	75,870,697 (GRCm39)	missense	probably benign	0.00
R7923:Mapk15	UTSW	15	75,868,295 (GRCm39)	missense	probably damaging	1.00
R9308:Mapk15	UTSW	15	75,865,714 (GRCm39)	nonsense	probably null
R9744:Mapk15	UTSW	15	75,869,912 (GRCm39)	missense	possibly damaging	0.78
Z1177:Mapk15	UTSW	15	75,870,310 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- ACCTTCCGTGAGATTATGCTTC -3'
(R):5'- GGCTTTGAAGTCAGTGGTCAC -3'

Sequencing Primer
(F):5'- ATGCTTCTCAAGGTAGGTGAC -3'
(R):5'- AAGTCAGTGGTCACCAGCTTTG -3'

Posted On

2015-08-18