Mutagenetix > Incidental Mutation

Incidental Mutation 'R4572:Wnk1'

342191

Institutional Source

Beutler Lab

Gene Symbol

Wnk1

Ensembl Gene

ENSMUSG00000045962

Gene Name

WNK lysine deficient protein kinase 1

Synonyms

6430573H23Rik, Hsn2, EG406236, Prkwnk1

MMRRC Submission

041796-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4572 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

119923969-120038672 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 119951911 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 1319 (T1319A)

Ref Sequence

ENSEMBL: ENSMUSP00000086017 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000060043] [ENSMUST00000088644] [ENSMUST00000088646] [ENSMUST00000160686] [ENSMUST00000203030] [ENSMUST00000177761]

AlphaFold

P83741

Predicted Effect

probably benign
Transcript: ENSMUST00000060043
AA Change: T1061A

PolyPhen 2 Score 0.052 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000063001
Gene: ENSMUSG00000045962
AA Change: T1061A

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.5e-44	PFAM
Pfam:Pkinase	221	479	4.4e-58	PFAM
Pfam:OSR1_C	500	537	2e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	865	876	N/A	INTRINSIC
low complexity region	1018	1028	N/A	INTRINSIC
low complexity region	1042	1058	N/A	INTRINSIC
internal_repeat_1	1136	1178	2.15e-5	PROSPERO
low complexity region	1289	1305	N/A	INTRINSIC
low complexity region	1345	1357	N/A	INTRINSIC
low complexity region	1379	1396	N/A	INTRINSIC
low complexity region	1398	1428	N/A	INTRINSIC
low complexity region	1429	1454	N/A	INTRINSIC
low complexity region	1496	1515	N/A	INTRINSIC
low complexity region	1542	1556	N/A	INTRINSIC
low complexity region	1586	1609	N/A	INTRINSIC
low complexity region	1670	1691	N/A	INTRINSIC
low complexity region	1698	1712	N/A	INTRINSIC
low complexity region	1738	1764	N/A	INTRINSIC
low complexity region	1882	1900	N/A	INTRINSIC
coiled coil region	2065	2092	N/A	INTRINSIC
low complexity region	2103	2114	N/A	INTRINSIC
low complexity region	2116	2140	N/A	INTRINSIC
low complexity region	2208	2232	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000088644
AA Change: T1319A

PolyPhen 2 Score 0.490 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000086017
Gene: ENSMUSG00000045962
AA Change: T1319A

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	5.5e-44	PFAM
Pfam:Pkinase	221	479	4.3e-56	PFAM
Pfam:OSR1_C	500	537	1.9e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	742	753	N/A	INTRINSIC
low complexity region	919	935	N/A	INTRINSIC
low complexity region	1050	1063	N/A	INTRINSIC
low complexity region	1067	1076	N/A	INTRINSIC
low complexity region	1146	1158	N/A	INTRINSIC
low complexity region	1276	1286	N/A	INTRINSIC
low complexity region	1300	1316	N/A	INTRINSIC
internal_repeat_1	1394	1436	2.19e-5	PROSPERO
low complexity region	1547	1563	N/A	INTRINSIC
low complexity region	1603	1615	N/A	INTRINSIC
low complexity region	1637	1654	N/A	INTRINSIC
low complexity region	1656	1686	N/A	INTRINSIC
low complexity region	1687	1712	N/A	INTRINSIC
low complexity region	1754	1773	N/A	INTRINSIC
low complexity region	1800	1814	N/A	INTRINSIC
low complexity region	1844	1867	N/A	INTRINSIC
low complexity region	1928	1949	N/A	INTRINSIC
low complexity region	1956	1970	N/A	INTRINSIC
low complexity region	1996	2022	N/A	INTRINSIC
low complexity region	2140	2158	N/A	INTRINSIC
coiled coil region	2323	2350	N/A	INTRINSIC
low complexity region	2361	2372	N/A	INTRINSIC
low complexity region	2374	2398	N/A	INTRINSIC
low complexity region	2466	2490	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000088646
AA Change: T907A

PolyPhen 2 Score 0.300 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000086019
Gene: ENSMUSG00000045962
AA Change: T907A

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.3e-44	PFAM
Pfam:Pkinase	221	479	4e-58	PFAM
Pfam:OSR1_C	500	537	1.8e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	864	874	N/A	INTRINSIC
low complexity region	888	904	N/A	INTRINSIC
internal_repeat_1	982	1024	7.56e-6	PROSPERO
low complexity region	1135	1151	N/A	INTRINSIC
low complexity region	1191	1203	N/A	INTRINSIC
low complexity region	1225	1242	N/A	INTRINSIC
low complexity region	1244	1274	N/A	INTRINSIC
low complexity region	1275	1300	N/A	INTRINSIC
low complexity region	1342	1361	N/A	INTRINSIC
low complexity region	1388	1402	N/A	INTRINSIC
low complexity region	1432	1455	N/A	INTRINSIC
low complexity region	1516	1537	N/A	INTRINSIC
low complexity region	1544	1558	N/A	INTRINSIC
low complexity region	1584	1610	N/A	INTRINSIC
low complexity region	1700	1718	N/A	INTRINSIC
coiled coil region	1883	1910	N/A	INTRINSIC
low complexity region	1921	1932	N/A	INTRINSIC
low complexity region	1934	1958	N/A	INTRINSIC
low complexity region	2026	2050	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000159397

Predicted Effect

unknown
Transcript: ENSMUST00000159785
AA Change: T201A

SMART Domains

Protein: ENSMUSP00000124905
Gene: ENSMUSG00000045962
AA Change: T201A

Domain	Start	End	E-Value	Type
low complexity region	159	169	N/A	INTRINSIC
low complexity region	183	199	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000160686

SMART Domains

Protein: ENSMUSP00000137334
Gene: ENSMUSG00000045962

Domain	Start	End	E-Value	Type
low complexity region	102	115	N/A	INTRINSIC
low complexity region	119	128	N/A	INTRINSIC
low complexity region	198	210	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000160769

Predicted Effect

probably benign
Transcript: ENSMUST00000161048

SMART Domains

Protein: ENSMUSP00000125345
Gene: ENSMUSG00000045962

Domain	Start	End	E-Value	Type
Blast:S_TKc	2	68	3e-34	BLAST
SCOP:d1phk__	3	70	2e-7	SMART
Pfam:OSR1_C	89	126	9.5e-21	PFAM
coiled coil region	151	185	N/A	INTRINSIC
low complexity region	323	335	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000161185

Predicted Effect

probably benign
Transcript: ENSMUST00000161802

SMART Domains

Protein: ENSMUSP00000124724
Gene: ENSMUSG00000045962

Domain	Start	End	E-Value	Type
low complexity region	24	50	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000162368
AA Change: T160A

SMART Domains

Protein: ENSMUSP00000125383
Gene: ENSMUSG00000045962
AA Change: T160A

Domain	Start	End	E-Value	Type
low complexity region	83	95	N/A	INTRINSIC
low complexity region	142	158	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000203030
AA Change: T812A

SMART Domains

Protein: ENSMUSP00000145304
Gene: ENSMUSG00000045962
AA Change: T812A

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	4.1e-44	PFAM
Pfam:Pkinase	221	479	3.2e-56	PFAM
Pfam:OSR1_C	500	537	1.5e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	793	809	N/A	INTRINSIC
internal_repeat_1	887	929	5.8e-6	PROSPERO
low complexity region	1040	1056	N/A	INTRINSIC
low complexity region	1096	1108	N/A	INTRINSIC
low complexity region	1130	1147	N/A	INTRINSIC
low complexity region	1149	1179	N/A	INTRINSIC
low complexity region	1180	1205	N/A	INTRINSIC
low complexity region	1247	1266	N/A	INTRINSIC
low complexity region	1293	1307	N/A	INTRINSIC
low complexity region	1337	1360	N/A	INTRINSIC
low complexity region	1421	1442	N/A	INTRINSIC
low complexity region	1449	1463	N/A	INTRINSIC
low complexity region	1489	1515	N/A	INTRINSIC
low complexity region	1633	1651	N/A	INTRINSIC
coiled coil region	1816	1843	N/A	INTRINSIC
low complexity region	1854	1865	N/A	INTRINSIC
low complexity region	1867	1891	N/A	INTRINSIC
low complexity region	1959	1983	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000177761
AA Change: T1310A

SMART Domains

Protein: ENSMUSP00000136777
Gene: ENSMUSG00000045962
AA Change: T1310A

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.7e-44	PFAM
Pfam:Pkinase	221	479	5.1e-58	PFAM
Pfam:OSR1_C	500	537	2.2e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	710	722	N/A	INTRINSIC
low complexity region	828	839	N/A	INTRINSIC
low complexity region	1005	1021	N/A	INTRINSIC
low complexity region	1136	1149	N/A	INTRINSIC
low complexity region	1153	1162	N/A	INTRINSIC
low complexity region	1232	1244	N/A	INTRINSIC
low complexity region	1291	1307	N/A	INTRINSIC
internal_repeat_1	1385	1427	1.91e-5	PROSPERO
low complexity region	1538	1554	N/A	INTRINSIC
low complexity region	1594	1606	N/A	INTRINSIC
low complexity region	1628	1645	N/A	INTRINSIC
low complexity region	1647	1677	N/A	INTRINSIC
low complexity region	1678	1703	N/A	INTRINSIC
low complexity region	1745	1764	N/A	INTRINSIC
low complexity region	1791	1805	N/A	INTRINSIC
low complexity region	1835	1858	N/A	INTRINSIC
low complexity region	1919	1940	N/A	INTRINSIC
low complexity region	1947	1961	N/A	INTRINSIC
low complexity region	1987	2013	N/A	INTRINSIC
low complexity region	2131	2149	N/A	INTRINSIC
coiled coil region	2314	2341	N/A	INTRINSIC
low complexity region	2352	2363	N/A	INTRINSIC
low complexity region	2365	2389	N/A	INTRINSIC
low complexity region	2457	2481	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000204207

Predicted Effect

probably benign
Transcript: ENSMUST00000162541

SMART Domains

Protein: ENSMUSP00000125193
Gene: ENSMUSG00000045962

Domain	Start	End	E-Value	Type
low complexity region	63	84	N/A	INTRINSIC
low complexity region	91	105	N/A	INTRINSIC
low complexity region	131	157	N/A	INTRINSIC
low complexity region	247	265	N/A	INTRINSIC

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.2%
20x: 95.0%

Validation Efficiency

100% (71/71)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the WNK subfamily of serine/threonine protein kinases. The encoded protein may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.[provided by RefSeq, May 2010]
PHENOTYPE: Homozygous mutant mice die before birth, whereas heterozygotes survive and exhibit decreased blood pressure. Mice homozygous for an allele that does not produce the kidney isoform exhibit a slight increase in systemic arterial diastolic blood pressure and reduced sensitivity to amiloride. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca6	A	T	11: 110,216,548 (GRCm38)	I747N	probably benign	Het
Adamtsl2	A	G	2: 27,083,256 (GRCm38)	Y97C	probably damaging	Het
Alox12e	A	G	11: 70,321,181 (GRCm38)		probably benign	Het
Alpk2	A	T	18: 65,281,004 (GRCm38)	S2014T	probably damaging	Het
Ankrd36	A	T	11: 5,689,340 (GRCm38)		probably null	Het
Apobec1	T	C	6: 122,581,397 (GRCm38)	D133G	probably damaging	Het
Arfgef1	A	T	1: 10,213,141 (GRCm38)	I181N	probably damaging	Het
C130060K24Rik	A	T	6: 65,454,991 (GRCm38)	M293L	probably benign	Het
Cilp2	A	G	8: 69,882,410 (GRCm38)	V646A	probably damaging	Het
Clasrp	A	G	7: 19,584,464 (GRCm38)		probably null	Het
Cnot2	T	C	10: 116,494,846 (GRCm38)	T423A	probably benign	Het
Crtap	T	C	9: 114,384,806 (GRCm38)	D227G	probably benign	Het
Cyp17a1	A	G	19: 46,670,551 (GRCm38)	F217S	probably damaging	Het
Ddx60	A	G	8: 61,987,421 (GRCm38)	M1036V	probably damaging	Het
Dnah11	A	T	12: 118,010,125 (GRCm38)	I2818N	probably benign	Het
Dok6	T	A	18: 89,473,947 (GRCm38)	I169F	possibly damaging	Het
Duox2	T	A	2: 122,281,726 (GRCm38)	R1326S	probably benign	Het
Epha10	A	G	4: 124,902,568 (GRCm38)	T357A	unknown	Het
Ephb2	A	C	4: 136,655,940 (GRCm38)	F942C	probably damaging	Het
Fscn3	T	A	6: 28,430,635 (GRCm38)		probably null	Het
Gabrb2	A	G	11: 42,593,917 (GRCm38)	N267S	possibly damaging	Het
Gabrr3	A	T	16: 59,461,638 (GRCm38)	Y452F	probably benign	Het
Gen1	A	G	12: 11,242,418 (GRCm38)	S457P	probably damaging	Het
Gm1993	C	T	X: 25,560,356 (GRCm38)	R77H	probably damaging	Het
Gm5592	G	A	7: 41,216,159 (GRCm38)		probably benign	Het
Hmbox1	A	G	14: 64,903,233 (GRCm38)		probably null	Het
Hus1	A	T	11: 9,007,617 (GRCm38)		probably null	Het
Ino80	G	A	2: 119,402,358 (GRCm38)	R1160W	probably damaging	Het
Kalrn	A	T	16: 34,392,042 (GRCm38)	F27L	probably damaging	Het
Kri1	A	T	9: 21,280,384 (GRCm38)	F187L	probably damaging	Het
Lekr1	A	T	3: 65,783,915 (GRCm38)		noncoding transcript	Het
Mapk15	T	C	15: 75,998,750 (GRCm38)		probably benign	Het
Mrgpre	G	A	7: 143,781,104 (GRCm38)	L221F	probably damaging	Het
Mrpl50	A	T	4: 49,514,399 (GRCm38)	S91T	possibly damaging	Het
Muc4	T	C	16: 32,753,802 (GRCm38)	I1226T	probably benign	Het
Mup15	A	G	4: 61,438,217 (GRCm38)		probably null	Het
Ncapd3	A	G	9: 27,094,615 (GRCm38)	D1469G	probably damaging	Het
Nlrp9b	G	A	7: 20,026,681 (GRCm38)		probably null	Het
Npy6r	A	T	18: 44,275,917 (GRCm38)	Y135F	probably benign	Het
Olfr862	A	C	9: 19,883,979 (GRCm38)	C109G	probably benign	Het
Phf14	G	A	6: 12,006,824 (GRCm38)	R825Q	probably damaging	Het
Pigg	A	G	5: 108,332,885 (GRCm38)	M379V	probably benign	Het
Plppr3	T	A	10: 79,866,063 (GRCm38)	Q315L	probably benign	Het
Plxnd1	C	A	6: 115,955,756 (GRCm38)	C1921F	probably damaging	Het
Ptger4	A	C	15: 5,243,133 (GRCm38)	S2A	probably benign	Het
Rab4a	A	T	8: 123,834,060 (GRCm38)	D196V	probably benign	Het
Rbck1	G	A	2: 152,318,733 (GRCm38)	Q428*	probably null	Het
Rgs14	A	T	13: 55,380,062 (GRCm38)	N266I	probably damaging	Het
Serpina1c	A	T	12: 103,898,708 (GRCm38)		probably benign	Het
Sesn3	G	A	9: 14,321,220 (GRCm38)	R263H	probably benign	Het
Slfn1	A	T	11: 83,121,463 (GRCm38)	D135V	probably benign	Het
Spata17	T	C	1: 187,193,996 (GRCm38)	K46E	possibly damaging	Het
Srcin1	A	C	11: 97,534,934 (GRCm38)	D432E	probably damaging	Het
Stxbp5	T	C	10: 9,838,144 (GRCm38)	E217G	probably damaging	Het
Terf2ip	A	G	8: 112,012,017 (GRCm38)	D179G	probably damaging	Het
Tll1	A	G	8: 64,056,309 (GRCm38)	F556L	possibly damaging	Het
Tmed4	CTCTTTCT	CTCT	11: 6,274,461 (GRCm38)		probably null	Het
Trappc9	G	T	15: 72,937,067 (GRCm38)	Q537K	possibly damaging	Het
Trim30a	G	T	7: 104,411,188 (GRCm38)	C460*	probably null	Het
Trim35	T	C	14: 66,307,873 (GRCm38)	Y298H	probably damaging	Het
Ugt3a1	T	A	15: 9,306,393 (GRCm38)	H209Q	probably benign	Het
Ulk4	T	C	9: 121,192,764 (GRCm38)	K627R	probably damaging	Het
Wnt9b	G	A	11: 103,732,155 (GRCm38)	R141C	probably damaging	Het
Zfp735	A	G	11: 73,689,785 (GRCm38)	M37V	probably benign	Het
Zmym1	A	T	4: 127,050,835 (GRCm38)	N186K	probably benign	Het

Other mutations in Wnk1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00901:Wnk1	APN	6	119,960,708 (GRCm38)	missense	probably damaging	1.00
IGL01364:Wnk1	APN	6	119,937,411 (GRCm38)	missense	probably damaging	1.00
IGL01715:Wnk1	APN	6	119,948,397 (GRCm38)	missense	probably damaging	1.00
IGL01951:Wnk1	APN	6	119,963,485 (GRCm38)	missense	probably damaging	0.99
IGL02043:Wnk1	APN	6	119,949,078 (GRCm38)	unclassified	probably benign
IGL02268:Wnk1	APN	6	119,937,373 (GRCm38)	nonsense	probably null
IGL02348:Wnk1	APN	6	119,963,328 (GRCm38)	missense	probably damaging	0.98
IGL02425:Wnk1	APN	6	119,963,454 (GRCm38)	missense	probably damaging	1.00
IGL02850:Wnk1	APN	6	119,937,862 (GRCm38)	missense	probably benign	0.26
IGL03160:Wnk1	APN	6	119,926,633 (GRCm38)	missense	probably damaging	1.00
IGL03387:Wnk1	APN	6	119,954,187 (GRCm38)	missense	possibly damaging	0.76
IGL03405:Wnk1	APN	6	119,953,895 (GRCm38)	missense	probably benign	0.41
Actor	UTSW	6	119,970,978 (GRCm38)	missense	probably damaging	1.00
Bad	UTSW	6	119,965,678 (GRCm38)	intron	probably benign
Blink	UTSW	6	120,002,273 (GRCm38)	nonsense	probably null
Knock	UTSW	6	119,943,878 (GRCm38)	splice site	probably null
Narrow	UTSW	6	119,992,408 (GRCm38)	missense	probably damaging	1.00
nictitate	UTSW	6	120,002,280 (GRCm38)	missense	possibly damaging	0.88
opportunity	UTSW	6	119,951,068 (GRCm38)	missense	probably damaging	1.00
path	UTSW	6	120,037,149 (GRCm38)	missense	probably damaging	0.99
Stormy	UTSW	6	119,992,447 (GRCm38)	missense	probably damaging	1.00
tear	UTSW	6	119,963,470 (GRCm38)	missense	probably damaging	0.98
Tic	UTSW	6	119,950,083 (GRCm38)	missense	probably damaging	1.00
Unforgiving	UTSW	6	119,948,282 (GRCm38)	missense	probably damaging	1.00
Window	UTSW	6	119,992,453 (GRCm38)	missense	probably damaging	1.00
Woke	UTSW	6	119,962,955 (GRCm38)	missense	probably benign	0.12
IGL03052:Wnk1	UTSW	6	119,944,799 (GRCm38)	splice site	probably benign
PIT4480001:Wnk1	UTSW	6	119,963,367 (GRCm38)	nonsense	probably null
R0044:Wnk1	UTSW	6	120,037,149 (GRCm38)	missense	probably damaging	0.99
R0207:Wnk1	UTSW	6	119,952,733 (GRCm38)	missense	probably damaging	1.00
R0333:Wnk1	UTSW	6	119,928,163 (GRCm38)	intron	probably benign
R0453:Wnk1	UTSW	6	119,963,151 (GRCm38)	missense	probably damaging	0.99
R0457:Wnk1	UTSW	6	119,969,332 (GRCm38)	missense	probably damaging	1.00
R0501:Wnk1	UTSW	6	119,962,803 (GRCm38)	missense	probably damaging	1.00
R0525:Wnk1	UTSW	6	119,926,564 (GRCm38)	missense	probably damaging	1.00
R0526:Wnk1	UTSW	6	119,951,992 (GRCm38)	missense	probably damaging	0.99
R0606:Wnk1	UTSW	6	119,926,683 (GRCm38)	missense	probably damaging	1.00
R0658:Wnk1	UTSW	6	119,948,505 (GRCm38)	missense	probably damaging	0.98
R1148:Wnk1	UTSW	6	119,952,006 (GRCm38)	splice site	probably benign
R1188:Wnk1	UTSW	6	119,948,709 (GRCm38)	nonsense	probably null
R1245:Wnk1	UTSW	6	119,948,457 (GRCm38)	missense	probably benign	0.26
R1449:Wnk1	UTSW	6	119,952,818 (GRCm38)	missense	probably damaging	1.00
R1469:Wnk1	UTSW	6	119,950,684 (GRCm38)	splice site	probably benign
R1869:Wnk1	UTSW	6	119,951,089 (GRCm38)	missense	probably damaging	1.00
R1871:Wnk1	UTSW	6	119,951,089 (GRCm38)	missense	probably damaging	1.00
R1928:Wnk1	UTSW	6	119,952,923 (GRCm38)	missense	probably damaging	1.00
R1959:Wnk1	UTSW	6	119,969,247 (GRCm38)	missense	probably damaging	0.98
R1961:Wnk1	UTSW	6	119,969,247 (GRCm38)	missense	probably damaging	0.98
R1964:Wnk1	UTSW	6	119,934,382 (GRCm38)	missense	possibly damaging	0.86
R1983:Wnk1	UTSW	6	119,937,578 (GRCm38)	missense	probably damaging	1.00
R2062:Wnk1	UTSW	6	119,928,157 (GRCm38)	splice site	probably null
R2144:Wnk1	UTSW	6	119,948,988 (GRCm38)	unclassified	probably benign
R2186:Wnk1	UTSW	6	119,948,567 (GRCm38)	missense	probably benign	0.26
R2281:Wnk1	UTSW	6	119,963,640 (GRCm38)	splice site	probably null
R2338:Wnk1	UTSW	6	119,969,534 (GRCm38)	missense	probably benign	0.42
R2420:Wnk1	UTSW	6	119,936,367 (GRCm38)	critical splice donor site	probably null
R3727:Wnk1	UTSW	6	119,992,453 (GRCm38)	missense	probably damaging	1.00
R3773:Wnk1	UTSW	6	120,002,280 (GRCm38)	missense	possibly damaging	0.88
R3836:Wnk1	UTSW	6	119,950,043 (GRCm38)	missense	probably damaging	1.00
R3837:Wnk1	UTSW	6	119,950,043 (GRCm38)	missense	probably damaging	1.00
R3847:Wnk1	UTSW	6	119,969,354 (GRCm38)	missense	possibly damaging	0.70
R3903:Wnk1	UTSW	6	119,949,051 (GRCm38)	missense	probably damaging	1.00
R4031:Wnk1	UTSW	6	119,951,068 (GRCm38)	missense	probably damaging	1.00
R4095:Wnk1	UTSW	6	119,948,126 (GRCm38)	missense	probably damaging	1.00
R4232:Wnk1	UTSW	6	119,949,261 (GRCm38)	missense	possibly damaging	0.90
R4422:Wnk1	UTSW	6	119,953,895 (GRCm38)	missense	probably benign	0.41
R4423:Wnk1	UTSW	6	119,926,426 (GRCm38)	missense	probably damaging	1.00
R4704:Wnk1	UTSW	6	119,965,744 (GRCm38)	missense	possibly damaging	0.83
R4755:Wnk1	UTSW	6	119,963,470 (GRCm38)	missense	probably damaging	0.98
R4812:Wnk1	UTSW	6	119,952,771 (GRCm38)	missense	probably benign	0.16
R4822:Wnk1	UTSW	6	119,962,438 (GRCm38)	missense	probably benign	0.02
R4879:Wnk1	UTSW	6	119,949,377 (GRCm38)	missense	probably damaging	1.00
R4970:Wnk1	UTSW	6	119,965,735 (GRCm38)	intron	probably benign
R5002:Wnk1	UTSW	6	119,937,963 (GRCm38)	missense	probably benign	0.13
R5037:Wnk1	UTSW	6	119,965,735 (GRCm38)	intron	probably benign
R5152:Wnk1	UTSW	6	120,002,280 (GRCm38)	missense	possibly damaging	0.88
R5257:Wnk1	UTSW	6	120,037,188 (GRCm38)	missense	probably benign	0.00
R5354:Wnk1	UTSW	6	119,968,523 (GRCm38)	missense	probably benign	0.01
R5421:Wnk1	UTSW	6	119,952,818 (GRCm38)	missense	probably damaging	1.00
R5564:Wnk1	UTSW	6	119,948,891 (GRCm38)	unclassified	probably benign
R5600:Wnk1	UTSW	6	119,949,358 (GRCm38)	missense	probably damaging	1.00
R5847:Wnk1	UTSW	6	119,992,408 (GRCm38)	missense	probably damaging	1.00
R6083:Wnk1	UTSW	6	120,037,601 (GRCm38)	missense	probably damaging	0.99
R6110:Wnk1	UTSW	6	119,972,997 (GRCm38)	intron	probably benign
R6128:Wnk1	UTSW	6	119,963,786 (GRCm38)	splice site	probably null
R6237:Wnk1	UTSW	6	119,952,767 (GRCm38)	missense	probably damaging	1.00
R6341:Wnk1	UTSW	6	119,948,585 (GRCm38)	missense	probably damaging	1.00
R6467:Wnk1	UTSW	6	119,962,955 (GRCm38)	missense	probably benign	0.12
R6696:Wnk1	UTSW	6	119,948,282 (GRCm38)	missense	probably damaging	1.00
R6888:Wnk1	UTSW	6	119,948,781 (GRCm38)	missense	probably benign	0.26
R6923:Wnk1	UTSW	6	119,965,678 (GRCm38)	intron	probably benign
R7024:Wnk1	UTSW	6	119,965,726 (GRCm38)	intron	probably benign
R7072:Wnk1	UTSW	6	119,937,861 (GRCm38)	missense	unknown
R7087:Wnk1	UTSW	6	120,037,530 (GRCm38)	missense	possibly damaging	0.94
R7102:Wnk1	UTSW	6	119,948,307 (GRCm38)	missense	unknown
R7134:Wnk1	UTSW	6	119,926,428 (GRCm38)	missense	unknown
R7137:Wnk1	UTSW	6	120,038,212 (GRCm38)	unclassified	probably benign
R7142:Wnk1	UTSW	6	119,949,279 (GRCm38)	missense	probably benign	0.09
R7174:Wnk1	UTSW	6	119,970,978 (GRCm38)	missense	probably damaging	1.00
R7205:Wnk1	UTSW	6	119,943,878 (GRCm38)	splice site	probably null
R7218:Wnk1	UTSW	6	120,002,273 (GRCm38)	nonsense	probably null
R7498:Wnk1	UTSW	6	119,927,196 (GRCm38)	missense	unknown
R7599:Wnk1	UTSW	6	119,929,828 (GRCm38)	missense	possibly damaging	0.83
R7615:Wnk1	UTSW	6	119,932,738 (GRCm38)	missense	probably benign	0.27
R7799:Wnk1	UTSW	6	119,949,176 (GRCm38)	missense	probably benign	0.04
R7979:Wnk1	UTSW	6	120,037,448 (GRCm38)	missense	probably damaging	1.00
R8075:Wnk1	UTSW	6	119,932,714 (GRCm38)	missense	probably damaging	0.99
R8331:Wnk1	UTSW	6	119,953,833 (GRCm38)	missense	probably benign	0.09
R8343:Wnk1	UTSW	6	119,963,493 (GRCm38)	missense	probably damaging	0.99
R8348:Wnk1	UTSW	6	119,929,999 (GRCm38)	splice site	probably null
R8359:Wnk1	UTSW	6	119,992,447 (GRCm38)	missense	probably damaging	1.00
R8424:Wnk1	UTSW	6	119,934,427 (GRCm38)	missense	unknown
R8519:Wnk1	UTSW	6	119,950,083 (GRCm38)	missense	probably damaging	1.00
R8930:Wnk1	UTSW	6	119,963,265 (GRCm38)	missense	probably damaging	0.99
R8932:Wnk1	UTSW	6	119,963,265 (GRCm38)	missense	probably damaging	0.99
R8933:Wnk1	UTSW	6	120,036,998 (GRCm38)	missense	probably damaging	1.00
R9005:Wnk1	UTSW	6	119,962,432 (GRCm38)	missense	probably damaging	1.00
R9147:Wnk1	UTSW	6	119,948,670 (GRCm38)	missense	unknown
R9148:Wnk1	UTSW	6	119,948,670 (GRCm38)	missense	unknown
R9354:Wnk1	UTSW	6	119,965,699 (GRCm38)	missense	unknown
R9379:Wnk1	UTSW	6	119,951,717 (GRCm38)	missense	probably damaging	1.00
X0064:Wnk1	UTSW	6	120,037,032 (GRCm38)	missense	possibly damaging	0.93
Z1177:Wnk1	UTSW	6	119,948,205 (GRCm38)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- GGATGATGGAGCATTCTCATTGC -3'
(R):5'- GGCTGAGTTCACACCAACAG -3'

Sequencing Primer
(F):5'- GATGGAGCATTCTCATTGCCATCAC -3'
(R):5'- GCAACTAGCTTTGGAAACTCTC -3'

Posted On

2015-09-24