Mutagenetix > Incidental Mutation

Incidental Mutation 'R4573:Trio'

342294

Institutional Source

Beutler Lab

Gene Symbol

Trio

Ensembl Gene

ENSMUSG00000022263

Gene Name

triple functional domain (PTPRF interacting)

Synonyms

Solo, 6720464I07Rik

MMRRC Submission

041598-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4573 (G1)

Quality Score

217

Status

Not validated

Chromosome

Chromosomal Location

27730651-28025848 bp(-) (GRCm38)

Type of Mutation

small deletion (1 aa in frame mutation)

DNA Base Change (assembly)

CCTTCTTCTTCT to CCTTCTTCT at 27772998 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000154653 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000090247] [ENSMUST00000226287] [ENSMUST00000226644] [ENSMUST00000227337]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000090247

SMART Domains

Protein: ENSMUSP00000087714
Gene: ENSMUSG00000022263

Domain	Start	End	E-Value	Type
low complexity region	2	40	N/A	INTRINSIC
SEC14	68	207	3.4e-26	SMART
SPEC	221	337	2.48e-9	SMART
SPEC	343	445	1.92e-15	SMART
SPEC	569	671	5.35e-14	SMART
SPEC	674	783	1.18e-6	SMART
SPEC	910	1011	2.6e-12	SMART
SPEC	1141	1243	7e-18	SMART
low complexity region	1249	1258	N/A	INTRINSIC
RhoGEF	1296	1466	2.79e-53	SMART
PH	1480	1593	1.53e-9	SMART
SH3	1659	1720	1.9e-8	SMART
low complexity region	1788	1802	N/A	INTRINSIC
low complexity region	1837	1863	N/A	INTRINSIC
low complexity region	1936	1954	N/A	INTRINSIC
RhoGEF	1973	2144	1.32e-63	SMART
PH	2158	2273	3.6e-6	SMART
low complexity region	2291	2341	N/A	INTRINSIC
low complexity region	2371	2390	N/A	INTRINSIC
low complexity region	2491	2503	N/A	INTRINSIC
SH3	2558	2619	1.04e0	SMART
low complexity region	2640	2660	N/A	INTRINSIC
IGc2	2701	2770	4e-12	SMART
S_TKc	2800	3054	4.84e-72	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000226287

Predicted Effect

probably benign
Transcript: ENSMUST00000226644

Predicted Effect

probably benign
Transcript: ENSMUST00000227337

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 95.1%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a large protein that functions as a GDP to GTP exchange factor. This protein promotes the reorganization of the actin cytoskeleton, thereby playing a role in cell migration and growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
PHENOTYPE: Homozygous mutant mice die during late embryonic development or shortly after birth. They exhibit abnormal skeletal myogenesis and display aberrant organization within the hippocampus and olfactory bulb. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 79 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam15	T	C	3: 89,345,986 (GRCm38)	E212G	probably damaging	Het
Adam1b	A	C	5: 121,500,793 (GRCm38)	S730A	probably benign	Het
Akr1b10	T	C	6: 34,392,129 (GRCm38)	V153A	probably damaging	Het
Ap1m2	T	C	9: 21,305,758 (GRCm38)	Y94C	probably damaging	Het
Arhgef33	T	C	17: 80,365,282 (GRCm38)	S320P	probably damaging	Het
Arsg	T	C	11: 109,517,282 (GRCm38)	S87P	probably damaging	Het
Asic3	A	G	5: 24,417,192 (GRCm38)	Y458C	probably damaging	Het
Aspm	G	T	1: 139,479,507 (GRCm38)	W2044L	probably damaging	Het
Atad2b	T	C	12: 4,954,663 (GRCm38)		probably null	Het
Atp1a2	T	C	1: 172,278,637 (GRCm38)	I869M	possibly damaging	Het
Bpifb2	C	T	2: 153,889,492 (GRCm38)	L263F	probably damaging	Het
C1s2	T	C	6: 124,628,243 (GRCm38)		probably null	Het
Carf	G	T	1: 60,148,112 (GRCm38)	A590S	probably benign	Het
Cbarp	G	T	10: 80,131,411 (GRCm38)	D658E	probably damaging	Het
Cd1d2	A	G	3: 86,987,554 (GRCm38)	I78V	probably benign	Het
Cdc42bpb	A	T	12: 111,323,141 (GRCm38)	M418K	probably benign	Het
Cdhr3	A	T	12: 33,068,153 (GRCm38)		probably null	Het
Cep290	A	G	10: 100,518,850 (GRCm38)	K932R	probably benign	Het
Ces1d	A	C	8: 93,181,534 (GRCm38)	N310K	probably benign	Het
Chka	A	G	19: 3,885,960 (GRCm38)	K240R	probably damaging	Het
Cltb	C	T	13: 54,598,761 (GRCm38)	R64H	probably damaging	Het
Cyp4f37	A	G	17: 32,629,087 (GRCm38)	E193G	probably benign	Het
Dhrs9	C	A	2: 69,397,641 (GRCm38)	H200N	probably benign	Het
Dnah6	T	A	6: 73,086,181 (GRCm38)	N2698I	probably damaging	Het
Dnah8	T	C	17: 30,700,406 (GRCm38)	S1118P	probably benign	Het
Dyrk1a	A	G	16: 94,692,023 (GRCm38)	Y705C	possibly damaging	Het
Elmod1	T	C	9: 53,925,972 (GRCm38)	N183S	probably damaging	Het
Fam129a	T	C	1: 151,703,766 (GRCm38)	V412A	possibly damaging	Het
Fer1l6	G	A	15: 58,626,280 (GRCm38)		probably null	Het
Fsip2	A	T	2: 82,986,166 (GRCm38)	Y4081F	possibly damaging	Het
Gm38394	A	G	1: 133,659,389 (GRCm38)	I70T	probably benign	Het
Gsr	A	G	8: 33,693,853 (GRCm38)	D381G	probably benign	Het
Gucy1a1	A	G	3: 82,108,922 (GRCm38)	L253S	possibly damaging	Het
Herc3	C	A	6: 58,894,113 (GRCm38)	T69K	possibly damaging	Het
Hoxb13	A	G	11: 96,194,951 (GRCm38)	Y170C	probably damaging	Het
Hsd3b5	T	C	3: 98,619,648 (GRCm38)	M161V	probably benign	Het
Lrp6	G	A	6: 134,470,730 (GRCm38)	R985*	probably null	Het
March5	T	A	19: 37,220,394 (GRCm38)	I154K	probably damaging	Het
Mcemp1	A	G	8: 3,665,835 (GRCm38)		probably null	Het
Mrpl2	G	T	17: 46,649,041 (GRCm38)	C212F	possibly damaging	Het
Mterf1a	G	A	5: 3,891,119 (GRCm38)	R250W	possibly damaging	Het
Mthfd1	A	G	12: 76,294,138 (GRCm38)		probably null	Het
Mul1	T	C	4: 138,436,349 (GRCm38)	F19L	probably benign	Het
Mycbp2	G	T	14: 103,346,297 (GRCm38)	A74E	probably benign	Het
Myo5a	A	T	9: 75,201,297 (GRCm38)		probably null	Het
Ncor2	A	T	5: 125,055,825 (GRCm38)	S33T	probably damaging	Het
Ninj1	A	G	13: 49,194,987 (GRCm38)	N191S	probably damaging	Het
Nrap	T	C	19: 56,342,338 (GRCm38)		probably null	Het
Ofcc1	G	A	13: 40,015,388 (GRCm38)	T841I	probably damaging	Het
Olfr353	T	C	2: 36,890,190 (GRCm38)	I219M	probably damaging	Het
Olfr50	T	A	2: 36,793,479 (GRCm38)	M81K	probably damaging	Het
Osbpl5	C	A	7: 143,694,316 (GRCm38)	V671L	probably benign	Het
Paics	T	C	5: 76,956,603 (GRCm38)	L25S	probably benign	Het
Pcmtd1	A	G	1: 7,120,367 (GRCm38)	E20G	probably damaging	Het
Pgbd5	G	A	8: 124,376,227 (GRCm38)	Q228*	probably null	Het
Pnpla7	T	C	2: 25,050,873 (GRCm38)	V1079A	probably damaging	Het
Pou2f1	G	A	1: 165,913,261 (GRCm38)	T113I	probably benign	Het
Ppp1r14c	A	G	10: 3,463,416 (GRCm38)	I150V	possibly damaging	Het
Ppp1r2	A	G	16: 31,260,637 (GRCm38)	Y115H	possibly damaging	Het
Ptchd4	T	C	17: 42,502,777 (GRCm38)	V523A	probably benign	Het
Rabep1	G	C	11: 70,917,751 (GRCm38)	S468T	probably damaging	Het
Rgs6	T	A	12: 83,066,015 (GRCm38)	W200R	probably damaging	Het
Ryr3	C	T	2: 112,755,174 (GRCm38)		probably null	Het
Scamp2	A	T	9: 57,577,194 (GRCm38)	D20V	probably damaging	Het
Sec24d	G	A	3: 123,358,870 (GRCm38)	V844M	probably damaging	Het
Sept10	T	A	10: 59,192,329 (GRCm38)	N57Y	probably damaging	Het
Sis	T	C	3: 72,928,237 (GRCm38)	K931E	possibly damaging	Het
Slamf7	T	C	1: 171,636,366 (GRCm38)	T258A	probably benign	Het
Slc15a1	A	G	14: 121,487,029 (GRCm38)	S144P	probably damaging	Het
Slc6a7	A	T	18: 61,002,181 (GRCm38)	V425E	probably benign	Het
Syt7	C	T	19: 10,439,212 (GRCm38)	R253*	probably null	Het
Tm4sf1	A	T	3: 57,294,785 (GRCm38)	C2S	possibly damaging	Het
Trpm3	C	T	19: 22,902,142 (GRCm38)	H594Y	probably damaging	Het
Tshz1	A	T	18: 84,015,082 (GRCm38)	N400K	probably damaging	Het
Vat1	A	T	11: 101,460,615 (GRCm38)	M300K	probably benign	Het
Vmn1r70	C	A	7: 10,633,629 (GRCm38)		probably null	Het
Vmn2r5	C	T	3: 64,503,918 (GRCm38)	D410N	probably damaging	Het
Yap1	T	C	9: 7,934,681 (GRCm38)	D428G	probably damaging	Het
Zfp157	A	G	5: 138,456,929 (GRCm38)	Y463C	probably damaging	Het

Other mutations in Trio

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00480:Trio	APN	15	27,912,743 (GRCm38)	splice site	probably benign
IGL01011:Trio	APN	15	27,736,489 (GRCm38)	missense	probably damaging	0.96
IGL01090:Trio	APN	15	27,773,007 (GRCm38)	missense	probably damaging	1.00
IGL01145:Trio	APN	15	27,818,167 (GRCm38)	splice site	probably benign
IGL01147:Trio	APN	15	27,881,320 (GRCm38)	missense	probably damaging	1.00
IGL01161:Trio	APN	15	27,749,781 (GRCm38)	missense	probably damaging	1.00
IGL01324:Trio	APN	15	27,905,323 (GRCm38)	missense	probably benign	0.42
IGL01352:Trio	APN	15	27,901,229 (GRCm38)	missense	probably benign	0.01
IGL01366:Trio	APN	15	27,732,868 (GRCm38)	missense	possibly damaging	0.76
IGL01443:Trio	APN	15	27,838,775 (GRCm38)	splice site	probably benign
IGL01454:Trio	APN	15	27,832,985 (GRCm38)	missense	probably benign	0.32
IGL01695:Trio	APN	15	27,773,001 (GRCm38)	missense	probably damaging	1.00
IGL01765:Trio	APN	15	27,764,026 (GRCm38)	missense	possibly damaging	0.85
IGL01860:Trio	APN	15	27,846,810 (GRCm38)	missense	probably damaging	1.00
IGL01879:Trio	APN	15	27,741,033 (GRCm38)	missense	probably benign	0.12
IGL01991:Trio	APN	15	27,871,274 (GRCm38)	missense	possibly damaging	0.95
IGL02106:Trio	APN	15	27,744,158 (GRCm38)	missense	possibly damaging	0.85
IGL02209:Trio	APN	15	27,744,053 (GRCm38)	missense	probably damaging	1.00
IGL02232:Trio	APN	15	27,902,561 (GRCm38)	missense	probably benign	0.24
IGL02304:Trio	APN	15	27,735,436 (GRCm38)	missense	probably damaging	0.96
IGL02504:Trio	APN	15	27,847,390 (GRCm38)	nonsense	probably null
IGL02508:Trio	APN	15	27,818,104 (GRCm38)	missense	possibly damaging	0.65
IGL02541:Trio	APN	15	27,844,930 (GRCm38)	splice site	probably benign
IGL02617:Trio	APN	15	27,841,849 (GRCm38)	splice site	probably benign
IGL02675:Trio	APN	15	27,768,039 (GRCm38)	unclassified	probably benign
IGL02817:Trio	APN	15	27,902,881 (GRCm38)	missense	probably benign	0.01
IGL02993:Trio	APN	15	27,830,239 (GRCm38)	splice site	probably benign
IGL03007:Trio	APN	15	27,902,742 (GRCm38)	missense	probably damaging	0.99
IGL03135:Trio	APN	15	27,832,011 (GRCm38)	splice site	probably benign
IGL03225:Trio	APN	15	27,902,695 (GRCm38)	missense	probably benign	0.30
R0063:Trio	UTSW	15	27,881,437 (GRCm38)	splice site	probably benign
R0063:Trio	UTSW	15	27,881,437 (GRCm38)	splice site	probably benign
R0302:Trio	UTSW	15	27,902,517 (GRCm38)	missense	probably damaging	1.00
R0505:Trio	UTSW	15	27,767,907 (GRCm38)	missense	probably benign	0.00
R0506:Trio	UTSW	15	27,854,963 (GRCm38)	missense	probably benign	0.12
R0564:Trio	UTSW	15	27,805,822 (GRCm38)	missense	probably damaging	1.00
R0659:Trio	UTSW	15	27,831,399 (GRCm38)	missense	probably damaging	0.97
R0882:Trio	UTSW	15	27,732,894 (GRCm38)	missense	probably damaging	1.00
R0939:Trio	UTSW	15	27,741,250 (GRCm38)	critical splice donor site	probably null
R1018:Trio	UTSW	15	27,871,171 (GRCm38)	missense	probably damaging	1.00
R1439:Trio	UTSW	15	27,897,914 (GRCm38)	missense	probably damaging	1.00
R1456:Trio	UTSW	15	27,753,804 (GRCm38)	splice site	probably benign
R1488:Trio	UTSW	15	27,740,967 (GRCm38)	missense	probably damaging	1.00
R1522:Trio	UTSW	15	27,732,640 (GRCm38)	missense	probably benign	0.28
R1531:Trio	UTSW	15	27,832,985 (GRCm38)	missense	probably benign	0.32
R1640:Trio	UTSW	15	27,833,044 (GRCm38)	missense	probably damaging	1.00
R1646:Trio	UTSW	15	27,758,347 (GRCm38)	missense	possibly damaging	0.91
R1682:Trio	UTSW	15	27,744,146 (GRCm38)	splice site	probably null
R1780:Trio	UTSW	15	27,744,038 (GRCm38)	missense	possibly damaging	0.93
R1791:Trio	UTSW	15	27,841,756 (GRCm38)	missense	probably damaging	1.00
R1803:Trio	UTSW	15	27,748,340 (GRCm38)	missense	probably benign
R1817:Trio	UTSW	15	27,742,495 (GRCm38)	nonsense	probably null
R1853:Trio	UTSW	15	27,756,536 (GRCm38)	missense	probably damaging	1.00
R1898:Trio	UTSW	15	27,742,380 (GRCm38)	missense	possibly damaging	0.52
R1937:Trio	UTSW	15	27,833,056 (GRCm38)	missense	probably damaging	1.00
R1938:Trio	UTSW	15	27,732,891 (GRCm38)	missense	probably damaging	0.98
R2025:Trio	UTSW	15	27,773,927 (GRCm38)	missense	probably damaging	1.00
R2025:Trio	UTSW	15	27,744,137 (GRCm38)	missense	probably damaging	0.99
R2050:Trio	UTSW	15	27,851,945 (GRCm38)	missense	possibly damaging	0.85
R2186:Trio	UTSW	15	27,823,975 (GRCm38)	splice site	probably null
R2913:Trio	UTSW	15	27,854,912 (GRCm38)	missense	probably damaging	1.00
R3151:Trio	UTSW	15	27,805,776 (GRCm38)	missense	probably damaging	1.00
R3771:Trio	UTSW	15	27,748,091 (GRCm38)	missense	probably damaging	0.98
R3773:Trio	UTSW	15	27,748,091 (GRCm38)	missense	probably damaging	0.98
R3826:Trio	UTSW	15	27,833,070 (GRCm38)	missense	probably damaging	1.00
R4015:Trio	UTSW	15	27,744,101 (GRCm38)	missense	possibly damaging	0.71
R4359:Trio	UTSW	15	27,749,797 (GRCm38)	nonsense	probably null
R4370:Trio	UTSW	15	27,748,337 (GRCm38)	nonsense	probably null
R4547:Trio	UTSW	15	27,818,982 (GRCm38)	missense	possibly damaging	0.89
R4620:Trio	UTSW	15	27,871,171 (GRCm38)	missense	probably damaging	1.00
R4735:Trio	UTSW	15	27,752,789 (GRCm38)	splice site	probably null
R4764:Trio	UTSW	15	27,732,538 (GRCm38)	nonsense	probably null
R4775:Trio	UTSW	15	27,881,342 (GRCm38)	nonsense	probably null
R4942:Trio	UTSW	15	27,752,725 (GRCm38)	missense	probably benign	0.21
R5004:Trio	UTSW	15	27,755,178 (GRCm38)	missense	probably damaging	1.00
R5149:Trio	UTSW	15	27,754,029 (GRCm38)	missense	possibly damaging	0.74
R5183:Trio	UTSW	15	27,902,600 (GRCm38)	missense	probably benign	0.00
R5186:Trio	UTSW	15	27,897,991 (GRCm38)	missense	probably damaging	0.97
R5268:Trio	UTSW	15	27,748,286 (GRCm38)	missense	probably benign	0.02
R5344:Trio	UTSW	15	27,735,532 (GRCm38)	missense	probably benign	0.12
R5407:Trio	UTSW	15	27,844,806 (GRCm38)	splice site	probably null
R5442:Trio	UTSW	15	27,856,194 (GRCm38)	missense	probably benign	0.04
R5617:Trio	UTSW	15	27,902,748 (GRCm38)	missense	probably benign
R5778:Trio	UTSW	15	27,856,164 (GRCm38)	missense	probably benign	0.33
R5986:Trio	UTSW	15	27,851,933 (GRCm38)	missense	possibly damaging	0.88
R5990:Trio	UTSW	15	27,891,459 (GRCm38)	missense	probably benign	0.10
R6011:Trio	UTSW	15	27,735,545 (GRCm38)	missense	probably damaging	0.98
R6063:Trio	UTSW	15	27,891,379 (GRCm38)	missense	possibly damaging	0.94
R6166:Trio	UTSW	15	27,818,071 (GRCm38)	missense	probably damaging	0.96
R6187:Trio	UTSW	15	27,743,952 (GRCm38)	critical splice donor site	probably null
R6387:Trio	UTSW	15	27,752,739 (GRCm38)	missense	probably damaging	1.00
R6402:Trio	UTSW	15	27,902,911 (GRCm38)	missense	probably benign	0.02
R6478:Trio	UTSW	15	27,856,107 (GRCm38)	missense	probably benign	0.01
R6528:Trio	UTSW	15	27,805,870 (GRCm38)	missense	probably damaging	1.00
R6662:Trio	UTSW	15	27,854,996 (GRCm38)	missense	probably benign	0.00
R6825:Trio	UTSW	15	27,889,308 (GRCm38)	missense	probably damaging	0.98
R6890:Trio	UTSW	15	27,919,288 (GRCm38)	unclassified	probably benign
R6945:Trio	UTSW	15	27,824,090 (GRCm38)	missense	probably damaging	1.00
R7027:Trio	UTSW	15	27,805,654 (GRCm38)	missense	possibly damaging	0.86
R7046:Trio	UTSW	15	27,832,051 (GRCm38)	missense	probably damaging	1.00
R7049:Trio	UTSW	15	27,749,799 (GRCm38)	missense	possibly damaging	0.66
R7075:Trio	UTSW	15	27,898,000 (GRCm38)	missense	unknown
R7094:Trio	UTSW	15	27,891,448 (GRCm38)	missense	unknown
R7123:Trio	UTSW	15	27,742,313 (GRCm38)	critical splice donor site	probably benign
R7130:Trio	UTSW	15	27,742,313 (GRCm38)	critical splice donor site	probably benign
R7214:Trio	UTSW	15	27,871,187 (GRCm38)	missense	probably damaging	0.97
R7292:Trio	UTSW	15	27,828,351 (GRCm38)	missense	possibly damaging	0.63
R7293:Trio	UTSW	15	27,871,289 (GRCm38)	missense	possibly damaging	0.66
R7352:Trio	UTSW	15	27,732,876 (GRCm38)	missense	probably damaging	0.96
R7426:Trio	UTSW	15	27,856,107 (GRCm38)	missense	probably benign	0.01
R7451:Trio	UTSW	15	27,747,913 (GRCm38)	missense	probably benign	0.07
R7558:Trio	UTSW	15	27,831,394 (GRCm38)	missense	possibly damaging	0.90
R7578:Trio	UTSW	15	27,854,939 (GRCm38)	missense	possibly damaging	0.94
R7596:Trio	UTSW	15	27,749,826 (GRCm38)	missense	probably damaging	0.99
R7604:Trio	UTSW	15	27,736,445 (GRCm38)	critical splice donor site	probably null
R7609:Trio	UTSW	15	27,912,642 (GRCm38)	missense	unknown
R7767:Trio	UTSW	15	27,889,418 (GRCm38)	missense	unknown
R7784:Trio	UTSW	15	27,763,994 (GRCm38)	missense	probably damaging	1.00
R7817:Trio	UTSW	15	27,749,866 (GRCm38)	missense	probably benign	0.35
R7833:Trio	UTSW	15	27,774,086 (GRCm38)	missense	probably damaging	0.99
R7873:Trio	UTSW	15	27,805,684 (GRCm38)	missense	possibly damaging	0.83
R7879:Trio	UTSW	15	27,851,924 (GRCm38)	missense	possibly damaging	0.94
R7989:Trio	UTSW	15	27,772,935 (GRCm38)	missense	probably damaging	0.97
R8022:Trio	UTSW	15	27,749,866 (GRCm38)	missense	probably benign	0.35
R8050:Trio	UTSW	15	27,891,454 (GRCm38)	missense	unknown
R8217:Trio	UTSW	15	27,818,969 (GRCm38)	missense	probably damaging	0.97
R8280:Trio	UTSW	15	27,902,910 (GRCm38)	missense	unknown
R8283:Trio	UTSW	15	27,756,542 (GRCm38)	missense	possibly damaging	0.79
R8300:Trio	UTSW	15	27,855,022 (GRCm38)	missense	possibly damaging	0.66
R8321:Trio	UTSW	15	27,881,326 (GRCm38)	missense	possibly damaging	0.90
R8477:Trio	UTSW	15	27,773,952 (GRCm38)	missense	possibly damaging	0.83
R8479:Trio	UTSW	15	27,901,200 (GRCm38)	missense	probably benign	0.25
R8682:Trio	UTSW	15	27,905,192 (GRCm38)	missense	unknown
R8688:Trio	UTSW	15	27,748,238 (GRCm38)	missense	possibly damaging	0.61
R8708:Trio	UTSW	15	27,732,546 (GRCm38)	missense	probably damaging	0.99
R8709:Trio	UTSW	15	27,919,237 (GRCm38)	missense	unknown
R8713:Trio	UTSW	15	27,743,951 (GRCm38)	critical splice donor site	probably benign
R8798:Trio	UTSW	15	27,851,837 (GRCm38)	missense	possibly damaging	0.92
R8812:Trio	UTSW	15	27,905,225 (GRCm38)	missense	unknown
R8816:Trio	UTSW	15	27,741,271 (GRCm38)	missense	probably damaging	0.96
R8828:Trio	UTSW	15	27,741,064 (GRCm38)	missense	possibly damaging	0.93
R8987:Trio	UTSW	15	27,732,687 (GRCm38)	missense	probably benign	0.23
R9051:Trio	UTSW	15	27,732,684 (GRCm38)	missense	possibly damaging	0.78
R9069:Trio	UTSW	15	27,852,011 (GRCm38)	missense	possibly damaging	0.83
R9075:Trio	UTSW	15	27,773,936 (GRCm38)	nonsense	probably null
R9079:Trio	UTSW	15	27,732,937 (GRCm38)	missense	possibly damaging	0.52
R9139:Trio	UTSW	15	27,749,836 (GRCm38)	nonsense	probably null
R9494:Trio	UTSW	15	27,846,757 (GRCm38)	missense	probably benign	0.00
R9680:Trio	UTSW	15	27,744,072 (GRCm38)	missense	possibly damaging	0.93
R9720:Trio	UTSW	15	27,847,409 (GRCm38)	missense	probably benign	0.00
R9726:Trio	UTSW	15	27,912,666 (GRCm38)	missense	unknown
X0024:Trio	UTSW	15	27,765,726 (GRCm38)	missense	possibly damaging	0.91
Z1176:Trio	UTSW	15	27,771,387 (GRCm38)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- AGAGTTCCCTGCTTCTGGTG -3'
(R):5'- AGTGTATGCTTGATCCCAGC -3'

Sequencing Primer
(F):5'- CTTCTGGTGGGTGCCTTCC -3'
(R):5'- GATCCCAGCCTCACTGTG -3'

Posted On

2015-09-24