Mutagenetix > Incidental Mutation

Incidental Mutation 'R4564:Gm10770'

343228

Institutional Source

Beutler Lab

Gene Symbol

Gm10770

Ensembl Gene

ENSMUSG00000074837

Gene Name

predicted gene 10770

Synonyms

MMRRC Submission

041789-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.194) question?

Stock #

R4564 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

150020802-150021496 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 150020831 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 229 (T229A)

Ref Sequence

ENSEMBL: ENSMUSP00000097015 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099418] [ENSMUST00000109926]

AlphaFold

F7D043

Predicted Effect

probably benign
Transcript: ENSMUST00000099418
AA Change: T229A

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

SMART Domains

Protein: ENSMUSP00000097015
Gene: ENSMUSG00000074837
AA Change: T229A

Domain	Start	End	E-Value	Type
ZnF_C2H2	12	34	8.09e0	SMART
ZnF_C2H2	40	62	8.4e1	SMART
ZnF_C2H2	68	90	1.12e-3	SMART
ZnF_C2H2	96	118	3.47e1	SMART
ZnF_C2H2	124	146	1.09e2	SMART
ZnF_C2H2	152	174	3.58e-2	SMART
ZnF_C2H2	180	202	6.23e-2	SMART
ZnF_C2H2	208	230	2.49e-1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000109926

SMART Domains

Protein: ENSMUSP00000105552
Gene: ENSMUSG00000079009

Domain	Start	End	E-Value	Type
Blast:KRAB	1	34	7e-15	BLAST
ZnF_C2H2	71	93	8.6e-5	SMART
ZnF_C2H2	99	121	1.76e-1	SMART
ZnF_C2H2	127	149	3.02e0	SMART
ZnF_C2H2	183	205	6.08e-5	SMART
ZnF_C2H2	211	233	1.04e-3	SMART
ZnF_C2H2	239	261	2.57e-3	SMART
ZnF_C2H2	267	289	1.06e-4	SMART
ZnF_C2H2	295	317	2.2e-2	SMART
ZnF_C2H2	323	345	4.47e-3	SMART
ZnF_C2H2	351	373	7.37e-4	SMART
ZnF_C2H2	379	401	4.24e-4	SMART
ZnF_C2H2	407	429	1.2e-3	SMART
ZnF_C2H2	435	457	2.61e-4	SMART

Meta Mutation Damage Score

0.3644

Coding Region Coverage

1x: 99.3%
3x: 98.5%
10x: 97.0%
20x: 94.6%

Validation Efficiency

97% (58/60)

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Atad3a	C	T	4: 155,831,766 (GRCm39)		probably null	Het
Bach2	T	G	4: 32,563,338 (GRCm39)	S602A	probably damaging	Het
Ccl19	G	T	4: 42,756,295 (GRCm39)	S12R	probably damaging	Het
Cfap54	T	C	10: 92,675,402 (GRCm39)		probably benign	Het
Cndp1	T	A	18: 84,640,411 (GRCm39)	I265F	probably damaging	Het
Cnot3	G	A	7: 3,656,257 (GRCm39)	R181H	probably damaging	Het
Dicer1	T	A	12: 104,671,010 (GRCm39)	K1011*	probably null	Het
Dip2b	G	A	15: 100,055,139 (GRCm39)	W99*	probably null	Het
Fpr-rs6	A	T	17: 20,403,168 (GRCm39)	Y64*	probably null	Het
Fubp1	C	A	3: 151,928,573 (GRCm39)	Y480*	probably null	Het
Gfra1	T	C	19: 58,227,682 (GRCm39)		probably null	Het
Gm826	A	C	2: 160,153,913 (GRCm39)		probably benign	Het
Gpd2	G	A	2: 57,197,095 (GRCm39)	V217I	possibly damaging	Het
Hectd4	T	C	5: 121,488,494 (GRCm39)	I3595T	probably benign	Het
Lmln	A	G	16: 32,930,226 (GRCm39)	E561G	probably benign	Het
Lrrc71	T	A	3: 87,652,715 (GRCm39)		probably benign	Het
Man2a2	T	A	7: 80,018,586 (GRCm39)	Y91F	probably benign	Het
Map4k4	A	G	1: 40,028,135 (GRCm39)	T319A	probably damaging	Het
Mcm6	G	T	1: 128,271,196 (GRCm39)	H474Q	probably damaging	Het
Mn1	C	G	5: 111,568,533 (GRCm39)	N834K	possibly damaging	Het
Mslnl	G	A	17: 25,961,908 (GRCm39)	V128M	probably damaging	Het
Niban3	T	C	8: 72,057,704 (GRCm39)		probably benign	Het
Npas2	A	G	1: 39,326,647 (GRCm39)	D44G	probably damaging	Het
Npc1	C	T	18: 12,324,789 (GRCm39)	G1235R	probably damaging	Het
Olfml2a	C	T	2: 38,850,306 (GRCm39)	T674I	probably benign	Het
Or4f58	A	G	2: 111,852,112 (GRCm39)	L29P	possibly damaging	Het
Pgap6	A	G	17: 26,336,837 (GRCm39)	R252G	possibly damaging	Het
Plxnb1	C	T	9: 108,942,488 (GRCm39)	A1779V	probably benign	Het
Ppil3	T	A	1: 58,470,481 (GRCm39)	D123V	probably damaging	Het
Prr23a3	A	G	9: 98,747,190 (GRCm39)	E48G	probably damaging	Het
Prr5l	T	C	2: 101,577,094 (GRCm39)	E110G	probably damaging	Het
Ptgir	A	G	7: 16,640,794 (GRCm39)	M29V	possibly damaging	Het
R3hdm1	C	T	1: 128,149,396 (GRCm39)	T839M	probably benign	Het
Rasgrf2	G	A	13: 92,033,773 (GRCm39)	Q544*	probably null	Het
Riok3	C	A	18: 12,281,936 (GRCm39)	R302S	probably damaging	Het
Rnf145	A	G	11: 44,439,635 (GRCm39)	K144E	probably benign	Het
Sin3b	A	C	8: 73,480,209 (GRCm39)	T904P	probably damaging	Het
Skint4	C	T	4: 111,977,066 (GRCm39)	T152M	probably damaging	Het
Slc22a2	A	G	17: 12,828,943 (GRCm39)	I350V	probably benign	Het
Slc6a11	G	T	6: 114,108,323 (GRCm39)	G29V	probably benign	Het
Speg	A	C	1: 75,368,478 (GRCm39)	H676P	probably damaging	Het
St6gal2	T	A	17: 55,789,648 (GRCm39)	H227Q	probably damaging	Het
Strn3	A	G	12: 51,680,404 (GRCm39)	S399P	probably benign	Het
Tbc1d1	A	G	5: 64,330,827 (GRCm39)	E2G	probably damaging	Het
Tecpr2	T	A	12: 110,921,219 (GRCm39)	M1264K	probably benign	Het
Trpm6	T	C	19: 18,809,961 (GRCm39)	L1119P	possibly damaging	Het
Upf2	A	C	2: 6,032,123 (GRCm39)	T890P	unknown	Het
Upk1b	T	A	16: 38,600,469 (GRCm39)	K170N	probably benign	Het
Vmn1r78	A	T	7: 11,886,485 (GRCm39)	Y32F	probably damaging	Het
Vps11	A	G	9: 44,272,894 (GRCm39)	F12S	probably damaging	Het
Zfp318	T	A	17: 46,723,741 (GRCm39)	C1915S	possibly damaging	Het
Zfp346	A	C	13: 55,261,520 (GRCm39)	R103S	probably damaging	Het

Other mutations in Gm10770

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
F5770:Gm10770	UTSW	2	150,021,404 (GRCm39)	nonsense	probably null
R1765:Gm10770	UTSW	2	150,021,258 (GRCm39)	missense	probably damaging	1.00
R1842:Gm10770	UTSW	2	150,021,076 (GRCm39)	missense	probably damaging	1.00
R3438:Gm10770	UTSW	2	150,021,469 (GRCm39)	splice site	probably null
R4863:Gm10770	UTSW	2	150,020,816 (GRCm39)	nonsense	probably null
R5134:Gm10770	UTSW	2	150,021,480 (GRCm39)	splice site	probably null
R5424:Gm10770	UTSW	2	150,020,948 (GRCm39)	missense	probably benign	0.01
R5429:Gm10770	UTSW	2	150,021,343 (GRCm39)	missense	probably benign	0.00
R5976:Gm10770	UTSW	2	150,021,320 (GRCm39)	nonsense	probably null
R6436:Gm10770	UTSW	2	150,020,830 (GRCm39)	missense	probably benign	0.07
R6679:Gm10770	UTSW	2	150,021,569 (GRCm39)	missense	probably damaging	1.00
V7580:Gm10770	UTSW	2	150,021,404 (GRCm39)	nonsense	probably null
V7581:Gm10770	UTSW	2	150,021,404 (GRCm39)	nonsense	probably null
V7583:Gm10770	UTSW	2	150,021,404 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- ATCCAGATGTCTCTCAACAGAC -3'
(R):5'- CTTTGCACATCAGTATGGTCTCT -3'

Sequencing Primer
(F):5'- TGTCTCTCAACAGACGAATGG -3'
(R):5'- GGTCAAGCCTTTAGAGTTCACAGTC -3'

Posted On

2015-09-24