Mutagenetix > Incidental Mutation

Incidental Mutation 'R4579:Miip'

343483

Institutional Source

Beutler Lab

Gene Symbol

Miip

Ensembl Gene

ENSMUSG00000029022

Gene Name

migration and invasion inhibitory protein

Synonyms

D4Wsu114e

MMRRC Submission

041801-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R4579 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

147860778-147868816 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 147861061 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Proline to Serine at position 373 (P373S)

Ref Sequence

ENSEMBL: ENSMUSP00000134085 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000030886] [ENSMUST00000119975] [ENSMUST00000172710]

AlphaFold

A2A7Y5

Predicted Effect

probably damaging
Transcript: ENSMUST00000030886
AA Change: P373S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000030886
Gene: ENSMUSG00000029022
AA Change: P373S

Domain	Start	End	E-Value	Type
low complexity region	13	24	N/A	INTRINSIC
low complexity region	60	69	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000119975

SMART Domains

Protein: ENSMUSP00000113897
Gene: ENSMUSG00000029022

Domain	Start	End	E-Value	Type
low complexity region	13	24	N/A	INTRINSIC
Pfam:MIIP	41	382	1.4e-142	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141166

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141534

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000156374

Predicted Effect

probably damaging
Transcript: ENSMUST00000172710
AA Change: P373S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000134085
Gene: ENSMUSG00000029022
AA Change: P373S

Domain	Start	End	E-Value	Type
low complexity region	13	24	N/A	INTRINSIC
low complexity region	60	69	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000173034

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.3%

Validation Efficiency

97% (88/91)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that interacts with the oncogene protein insulin-like growth factor binding protein 2 and may function as an inhibitor of cell migration and invasion. This protein also interacts with the cell division protein 20 and may be involved in regulating mitotic progression. This protein may function as a tumor suppressor by inhibiting the growth or certain cancers. [provided by RefSeq, Sep 2011]

Allele List at MGI

Other mutations in this stock

Total: 79 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A930004D18Rik	A	G	2: 18,027,037	I90T	probably damaging	Het
Acox3	A	G	5: 35,604,643	N444D	probably damaging	Het
Adamts16	A	G	13: 70,779,624	Y499H	probably damaging	Het
Ank2	A	T	3: 126,958,963	V368D	probably damaging	Het
Atad5	T	C	11: 80,095,191	V368A	probably damaging	Het
Atp13a5	T	C	16: 29,248,338		probably null	Het
Bdh1	A	T	16: 31,436,136		probably benign	Het
Bmp6	A	T	13: 38,469,725	Y256F	probably damaging	Het
Bub1b	C	A	2: 118,623,176	S496*	probably null	Het
Capn15	C	T	17: 25,959,837	R1128H	probably damaging	Het
Ccnf	T	A	17: 24,231,329	R461*	probably null	Het
Col6a1	C	T	10: 76,711,357	V725I	unknown	Het
Cp	T	C	3: 19,957,435		probably null	Het
Cul2	T	C	18: 3,430,957	V577A	probably benign	Het
Cux2	A	T	5: 121,860,653	I1408K	probably benign	Het
Cyp2c69	T	A	19: 39,881,186	T130S	possibly damaging	Het
Cyp3a57	A	G	5: 145,374,264	T278A	probably benign	Het
Dchs1	T	G	7: 105,754,765	T2857P	probably damaging	Het
Dchs1	A	G	7: 105,758,973	M1884T	probably benign	Het
Dnah3	T	C	7: 120,009,331	S1802G	probably damaging	Het
Dner	T	C	1: 84,383,816	S691G	probably damaging	Het
Dzip1l	A	T	9: 99,647,214	Q332L	probably damaging	Het
Eprs	A	G	1: 185,401,607	Y827C	probably damaging	Het
Ermp1	A	T	19: 29,616,651	N706K	probably damaging	Het
F830045P16Rik	A	G	2: 129,463,503	L317S	probably damaging	Het
Fam120c	G	T	X: 151,436,183	G696W	probably damaging	Het
Fance	T	A	17: 28,317,151		probably null	Het
Fancl	G	T	11: 26,468,423		probably null	Het
Fbxo30	T	A	10: 11,289,549	V5E	probably benign	Het
Foxn4	A	T	5: 114,256,825	I347N	possibly damaging	Het
Galr2	A	T	11: 116,281,499	D5V	probably benign	Het
Gm14139	T	A	2: 150,192,223	Y186N	probably damaging	Het
Gm4845	T	G	1: 141,257,127		noncoding transcript	Het
Gm8909	C	T	17: 36,161,757		probably benign	Het
Gm996	T	A	2: 25,579,649	R83S	possibly damaging	Het
Gnao1	A	T	8: 93,966,904	Q73L	probably damaging	Het
Gnpat	T	A	8: 124,878,502		probably null	Het
Hectd1	A	G	12: 51,744,573	M2594T	probably damaging	Het
Hint3	T	C	10: 30,610,432	H117R	probably damaging	Het
Hsd3b6	A	G	3: 98,806,225	F253L	probably damaging	Het
Itgal	A	G	7: 127,305,294	D313G	possibly damaging	Het
Kbtbd2	A	T	6: 56,778,908	D614E	probably damaging	Het
Ksr2	A	T	5: 117,756,270	I825F	probably damaging	Het
L3mbtl4	A	T	17: 68,764,640	S521C	probably benign	Het
Lamc1	T	C	1: 153,247,269	N725S	probably damaging	Het
Med1	T	G	11: 98,158,422	E516A	possibly damaging	Het
Mical3	A	T	6: 120,958,699	V1622E	probably benign	Het
Myo7a	C	A	7: 98,073,193	S1175I	probably damaging	Het
Ndst3	A	T	3: 123,546,825	D781E	probably benign	Het
Nf1	T	A	11: 79,468,757	V1407E	probably damaging	Het
Olfr1066	T	C	2: 86,455,515	Y252C	probably damaging	Het
Olfr1216	T	C	2: 89,013,144	I307V	probably benign	Het
Olfr133	G	C	17: 38,149,405	K272N	probably damaging	Het
Olfr282	A	T	15: 98,437,679	D70V	probably damaging	Het
Olfr452	A	T	6: 42,790,524	I162F	probably benign	Het
Olfr870	G	T	9: 20,171,114	S152R	probably damaging	Het
Opalin	A	G	19: 41,067,757	L33P	probably damaging	Het
Pcdhb2	T	A	18: 37,296,115	N23K	probably damaging	Het
Pdap1	A	G	5: 145,136,881		probably benign	Het
Pds5b	A	G	5: 150,746,732	E395G	probably damaging	Het
Pex1	C	A	5: 3,618,880	R624S	probably benign	Het
Pitrm1	G	A	13: 6,558,225	V329I	probably benign	Het
Pop1	G	A	15: 34,515,824		probably benign	Het
Prkaa1	A	G	15: 5,160,601		probably null	Het
Slc38a6	G	T	12: 73,288,524		probably null	Het
Slc5a9	T	A	4: 111,893,187	Y158F	probably damaging	Het
Spata3	T	A	1: 86,026,453	V114E	probably damaging	Het
Tbcb	T	A	7: 30,231,594	I34F	possibly damaging	Het
Tek	T	A	4: 94,863,666	Y1014*	probably null	Het
Togaram1	G	T	12: 64,967,907	C644F	probably damaging	Het
Trim56	A	C	5: 137,114,064	D199E	possibly damaging	Het
Tssk3	A	T	4: 129,489,317	D187E	probably benign	Het
Ttn	G	A	2: 76,896,803		probably benign	Het
Twf2	G	A	9: 106,212,826	R126Q	probably benign	Het
Ugt1a10	T	C	1: 88,056,116	M212T	probably benign	Het
Vmn1r177	A	T	7: 23,866,347	F35I	possibly damaging	Het
Vmn1r44	T	A	6: 89,893,933	H77Q	possibly damaging	Het
Zfp524	G	A	7: 5,018,348	V292I	probably benign	Het
Zfp788	T	G	7: 41,647,594	I56S	probably benign	Het

Other mutations in Miip

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00531:Miip	APN	4	147865865	missense	probably damaging	1.00
IGL02134:Miip	APN	4	147865278	splice site	probably benign
IGL02829:Miip	APN	4	147863061	missense	probably benign	0.01
IGL03350:Miip	APN	4	147862522	missense	probably benign	0.01
R0200:Miip	UTSW	4	147862263	missense	probably damaging	0.99
R1647:Miip	UTSW	4	147865234	missense	probably benign	0.02
R1783:Miip	UTSW	4	147865774	missense	probably damaging	1.00
R1848:Miip	UTSW	4	147863092	missense	probably damaging	0.99
R1944:Miip	UTSW	4	147865965	missense	probably benign	0.15
R3615:Miip	UTSW	4	147865914	missense	probably benign	0.00
R3616:Miip	UTSW	4	147865914	missense	probably benign	0.00
R3882:Miip	UTSW	4	147861052	missense	possibly damaging	0.93
R5183:Miip	UTSW	4	147863069	missense	probably damaging	1.00
R6054:Miip	UTSW	4	147865678	missense	probably benign	0.00
R6056:Miip	UTSW	4	147862335	missense	probably damaging	1.00
R6304:Miip	UTSW	4	147863083	missense	probably benign	0.12
R6568:Miip	UTSW	4	147865915	missense	probably benign
R6603:Miip	UTSW	4	147865923	missense	possibly damaging	0.92
R7639:Miip	UTSW	4	147862564	missense	probably benign	0.22
R7701:Miip	UTSW	4	147862914	missense	probably null	0.86
R7795:Miip	UTSW	4	147862918	missense	probably benign	0.17
R7796:Miip	UTSW	4	147862918	missense	probably benign	0.17
R7797:Miip	UTSW	4	147862918	missense	probably benign	0.17
R7872:Miip	UTSW	4	147862918	missense	probably benign	0.17
R7920:Miip	UTSW	4	147862918	missense	probably benign	0.17
R8468:Miip	UTSW	4	147861471	missense	probably damaging	1.00
R8492:Miip	UTSW	4	147861424	missense	probably damaging	1.00
R8677:Miip	UTSW	4	147863046	missense	probably damaging	1.00
R8852:Miip	UTSW	4	147866382	start gained	probably benign
R8860:Miip	UTSW	4	147866382	start gained	probably benign
R9755:Miip	UTSW	4	147865862	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- TAGCACGCTACTCAGAGCAC -3'
(R):5'- TGACACTCCTTGACACCTGC -3'

Sequencing Primer
(F):5'- TTTCAGTATCTCAGTAAACAGAGGGG -3'
(R):5'- CTCCAGTCTCGCCAGGAAAG -3'

Posted On

2015-09-24