Mutagenetix > Incidental Mutation

Incidental Mutation 'R4581:Or8k35'

343644

Institutional Source

Beutler Lab

Gene Symbol

Or8k35

Ensembl Gene

ENSMUSG00000111689

Gene Name

olfactory receptor family 8 subfamily K member 35

Synonyms

MOR192-4_p, GA_x6K02T2Q125-48079993-48079157, Olfr1082

MMRRC Submission

041802-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.398) question?

Stock #

R4581 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

86424229-86429153 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 86424572 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 200 (M200K)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000215600]

AlphaFold

A0A1L1SUC9

Predicted Effect

probably benign
Transcript: ENSMUST00000111578
AA Change: M200K

PolyPhen 2 Score 0.075 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000107204
Gene: ENSMUSG00000079239
AA Change: M200K

Domain	Start	End	E-Value	Type
Pfam:7tm_1	41	290	5.6e-31	PFAM
Pfam:7tm_4	139	283	4.5e-45	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000215600
AA Change: M200K

PolyPhen 2 Score 0.061 (Sensitivity: 0.94; Specificity: 0.84)

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 95.1%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 71 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3425401B19Rik	G	T	14: 32,383,828 (GRCm39)	S712R	possibly damaging	Het
4933427I04Rik	A	G	4: 123,754,146 (GRCm39)	D20G	possibly damaging	Het
Abca7	A	G	10: 79,842,402 (GRCm39)	D1112G	probably benign	Het
Actc1	G	T	2: 113,880,089 (GRCm39)	H175N	possibly damaging	Het
Adgrd1	C	T	5: 129,279,595 (GRCm39)	A863V	possibly damaging	Het
Ankrd17	T	C	5: 90,430,979 (GRCm39)	D935G	possibly damaging	Het
Ankrd7	T	A	6: 18,868,020 (GRCm39)	N95K	probably damaging	Het
Arhgef4	G	A	1: 34,771,205 (GRCm39)	E1171K	possibly damaging	Het
Ascc3	T	C	10: 50,587,121 (GRCm39)	I991T	probably damaging	Het
Cacna1s	T	A	1: 135,998,708 (GRCm39)		probably null	Het
Camk1d	A	T	2: 5,359,515 (GRCm39)	V177E	probably benign	Het
Cdh18	T	A	15: 23,226,869 (GRCm39)	I110N	probably damaging	Het
Cenpe	A	G	3: 134,952,761 (GRCm39)	K1484E	probably benign	Het
Cep68	A	G	11: 20,189,333 (GRCm39)	S560P	probably benign	Het
Cog3	G	T	14: 75,970,391 (GRCm39)	T352K	probably benign	Het
Cox6a2	A	G	7: 127,805,152 (GRCm39)	S44P	possibly damaging	Het
Cplane1	C	A	15: 8,201,282 (GRCm39)	P20T	possibly damaging	Het
Csmd2	T	C	4: 128,262,881 (GRCm39)	V689A	probably benign	Het
Ddx60	A	T	8: 62,476,295 (GRCm39)	M1548L	possibly damaging	Het
Dennd5b	G	T	6: 148,918,482 (GRCm39)		silent	Het
Dlgap2	T	C	8: 14,896,679 (GRCm39)	Y1052H	probably damaging	Het
Dnaaf5	T	A	5: 139,170,440 (GRCm39)	D502E	probably damaging	Het
Efhb	C	A	17: 53,733,303 (GRCm39)	A523S	probably damaging	Het
Epha8	C	T	4: 136,660,775 (GRCm39)	V648M	probably damaging	Het
Fanca	C	T	8: 124,001,077 (GRCm39)		probably null	Het
Fbxw7	G	A	3: 84,874,852 (GRCm39)	E205K	probably benign	Het
Fer1l6	C	T	15: 58,512,075 (GRCm39)	T1514I	probably damaging	Het
Gm12886	T	C	4: 121,273,880 (GRCm39)	E112G	probably damaging	Het
Gm7535	T	C	17: 18,131,345 (GRCm39)		probably benign	Het
Irf2bp2	T	A	8: 127,317,994 (GRCm39)	Q524L	probably damaging	Het
Itih4	A	G	14: 30,622,925 (GRCm39)	D864G	probably benign	Het
Kdm4c	T	G	4: 74,275,576 (GRCm39)		probably null	Het
Ltn1	A	T	16: 87,198,912 (GRCm39)		probably null	Het
Mafa	G	T	15: 75,619,585 (GRCm39)	P63T	unknown	Het
Mars2	T	A	1: 55,277,021 (GRCm39)	L208H	probably damaging	Het
Myom2	A	G	8: 15,156,459 (GRCm39)	I769V	probably benign	Het
Nyap1	A	G	5: 137,734,284 (GRCm39)	S250P	probably damaging	Het
Osmr	C	T	15: 6,872,375 (GRCm39)	V240I	probably benign	Het
Pcdhga3	A	G	18: 37,809,934 (GRCm39)	T796A	probably benign	Het
Pclo	T	C	5: 14,725,519 (GRCm39)	V1459A	unknown	Het
Phactr3	A	C	2: 177,924,965 (GRCm39)	H300P	probably damaging	Het
Pla2g4e	T	G	2: 120,016,863 (GRCm39)	H226P	possibly damaging	Het
Plcd4	T	A	1: 74,587,383 (GRCm39)	W48R	probably damaging	Het
Prdm16	A	G	4: 154,407,810 (GRCm39)	S1140P	probably damaging	Het
Rarg	A	C	15: 102,160,986 (GRCm39)	S18A	possibly damaging	Het
Rfx4	T	A	10: 84,680,164 (GRCm39)	S114T	possibly damaging	Het
Sec14l4	G	A	11: 3,993,375 (GRCm39)		probably null	Het
Six1	T	G	12: 73,092,708 (GRCm39)	T165P	probably benign	Het
Skint4	T	C	4: 111,944,239 (GRCm39)	L17P	probably damaging	Het
Slc25a23	T	A	17: 57,359,740 (GRCm39)	Y337F	probably damaging	Het
Slc9a3	A	G	13: 74,312,284 (GRCm39)	Y627C	probably damaging	Het
Smu1	A	C	4: 40,737,401 (GRCm39)		probably null	Het
Spryd3	A	G	15: 102,038,799 (GRCm39)	S141P	probably damaging	Het
Src	A	T	2: 157,304,958 (GRCm39)	N175I	probably damaging	Het
Srcap	GCTCCTCCTCCTCCTCCT	GCTCCTCCTCCTCCT	7: 127,157,482 (GRCm39)		probably benign	Het
Stc2	A	G	11: 31,315,326 (GRCm39)		probably null	Het
Taf6l	T	C	19: 8,755,572 (GRCm39)	D261G	probably damaging	Het
Tal1	T	G	4: 114,921,919 (GRCm39)	V167G	probably damaging	Het
Tfec	G	A	6: 16,834,124 (GRCm39)	T261I	probably damaging	Het
Tgfb1	G	T	7: 25,396,655 (GRCm39)	S273I	possibly damaging	Het
Tmem8b	T	A	4: 43,685,760 (GRCm39)	V636E	probably damaging	Het
Top2b	T	G	14: 16,409,189 (GRCm38)	I777M	probably damaging	Het
Trappc11	A	T	8: 47,946,380 (GRCm39)	M1084K	probably damaging	Het
Trem3	A	C	17: 48,556,639 (GRCm39)	T37P	possibly damaging	Het
Ttc7b	G	A	12: 100,466,376 (GRCm39)	R79C	probably damaging	Het
Urb1	T	C	16: 90,585,034 (GRCm39)	D529G	probably benign	Het
Vmn2r8	T	A	5: 108,949,570 (GRCm39)	T426S	probably benign	Het
Yipf4	T	C	17: 74,806,089 (GRCm39)	Y243H	probably benign	Het
Zfp574	T	A	7: 24,780,738 (GRCm39)	C587S	probably damaging	Het
Zfp93	C	A	7: 23,975,093 (GRCm39)	H359Q	probably damaging	Het
Znfx1	T	C	2: 166,892,236 (GRCm39)	E660G	probably damaging	Het

Other mutations in Or8k35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0483:Or8k35	UTSW	2	86,424,752 (GRCm39)	missense	probably benign	0.07
R0675:Or8k35	UTSW	2	86,424,423 (GRCm39)	missense	probably benign	0.00
R0718:Or8k35	UTSW	2	86,424,425 (GRCm39)	missense	probably benign	0.01
R0727:Or8k35	UTSW	2	86,424,724 (GRCm39)	nonsense	probably null
R1517:Or8k35	UTSW	2	86,424,948 (GRCm39)	missense	probably damaging	0.99
R1850:Or8k35	UTSW	2	86,424,448 (GRCm39)	nonsense	probably null
R2430:Or8k35	UTSW	2	86,425,052 (GRCm39)	missense	probably benign	0.00
R2474:Or8k35	UTSW	2	86,424,957 (GRCm39)	missense	probably benign	0.00
R3009:Or8k35	UTSW	2	86,424,714 (GRCm39)	missense	probably benign	0.07
R3122:Or8k35	UTSW	2	86,424,954 (GRCm39)	missense	possibly damaging	0.91
R4006:Or8k35	UTSW	2	86,424,908 (GRCm39)	missense	probably benign	0.14
R4007:Or8k35	UTSW	2	86,424,908 (GRCm39)	missense	probably benign	0.14
R4762:Or8k35	UTSW	2	86,424,381 (GRCm39)	missense	possibly damaging	0.60
R5617:Or8k35	UTSW	2	86,424,345 (GRCm39)	missense	probably benign	0.07
R6118:Or8k35	UTSW	2	86,424,758 (GRCm39)	missense	probably benign
R6140:Or8k35	UTSW	2	86,424,448 (GRCm39)	nonsense	probably null
R6313:Or8k35	UTSW	2	86,424,411 (GRCm39)	missense	possibly damaging	0.76
R6528:Or8k35	UTSW	2	86,424,809 (GRCm39)	missense	probably damaging	1.00
R6785:Or8k35	UTSW	2	86,424,765 (GRCm39)	missense	probably damaging	0.98
R6792:Or8k35	UTSW	2	86,424,283 (GRCm39)	missense	probably benign	0.09
R6857:Or8k35	UTSW	2	86,424,608 (GRCm39)	missense	probably damaging	1.00
R6998:Or8k35	UTSW	2	86,424,488 (GRCm39)	missense	probably damaging	1.00
R7241:Or8k35	UTSW	2	86,424,498 (GRCm39)	missense	possibly damaging	0.89
R8790:Or8k35	UTSW	2	86,424,278 (GRCm39)	missense	possibly damaging	0.76
R8865:Or8k35	UTSW	2	86,424,744 (GRCm39)	missense	possibly damaging	0.89
R9521:Or8k35	UTSW	2	86,424,771 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- CTGTAGATCAGGGGATTCAATAAGG -3'
(R):5'- TGGCCATATGTAAGCCTCTGC -3'

Sequencing Primer
(F):5'- GTATCAATTGAATGGCTAGATACAGG -3'
(R):5'- AAGTATGTTGGGTTCTAGTCACAATG -3'

Posted On

2015-09-24