|Institutional Source||Beutler Lab|
|Gene Name||CD8 antigen, beta chain 1|
|Synonyms||Ly-3, Ly-C, Lyt-3|
|Is this an essential gene?||Probably non essential (E-score: 0.082)|
|Stock #||R4583 (G1)|
|Chromosomal Location||71322788-71337494 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to A at 71326097 bp (GRCm38)|
|Amino Acid Change||Isoleucine to Asparagine at position 52 (I52N)|
|Ref Sequence||ENSEMBL: ENSMUSP00000070131 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000065248] [ENSMUST00000114188] [ENSMUST00000173297]|
Crystal structure of a CD8ab heterodimer [X-RAY DIFFRACTION]
Crystal structure of CD8alpha-beta in complex with YTS 156.7 FAB [X-RAY DIFFRACTION]
Crystal structure of the CD8 alpha beta/H-2Dd complex [X-RAY DIFFRACTION]
AA Change: I52N
PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
AA Change: I52N
|Coding Region Coverage||
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. [provided by RefSeq, May 2010]
PHENOTYPE: Animals homozygous for a mutation in this gene lack CD8+CD4- cytotoxic T cells in the thymus and spleen and do not mount a cytotoxic response to alloantigens. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Cd8b1||
(F):5'- CATTGTGACACAAACCACTGT -3'
(R):5'- TCCTCTGGCTTCACATTCATGAT -3'
(F):5'- GGCAACCAAACTTGAAGTTCTGGTC -3'
(R):5'- CTGGCTTCACATTCATGATACTGAG -3'