|Institutional Source||Beutler Lab|
|Gene Name||matrix metallopeptidase 7|
|Is this an essential gene?||Possibly non essential (E-score: 0.252)|
|Stock #||R4595 (G1)|
|Chromosomal Location||7692090-7699585 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to C at 7697666 bp|
|Amino Acid Change||Valine to Alanine at position 234 (V234A)|
|Ref Sequence||ENSEMBL: ENSMUSP00000018767 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000018767]|
|Predicted Effect||probably damaging
AA Change: V234A
PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
AA Change: V234A
|Predicted Effect||noncoding transcript
|Meta Mutation Damage Score||0.4358|
|Coding Region Coverage||
|Validation Efficiency||94% (62/66)|
FUNCTION: This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme. Mice lacking the encoded protein are deficient in functional cryptdins. This gene is located in a cluster of other matrix metalloproteinase genes on chromosome 9. [provided by RefSeq, Feb 2016]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit increased sensitivity to induced colitis and corneal wound neovascularization, decreased sensitivity to myocardial infarction and pancreatic duct ligation, impaired tracheal wound healing, and altered tumor susceptibility. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Mmp7||
(F):5'- CCAATAACTGAGGAAACCTGGG -3'
(R):5'- GGCGAATCTTGCATTCTGCTG -3'
(F):5'- CTGAGGAAACCTGGGGACTAG -3'
(R):5'- AGTGCATAGGTTTTCAGTCCC -3'