Mutagenetix > Incidental Mutation

Incidental Mutation 'R4610:Ltbp4'

344606

Institutional Source

Beutler Lab

Gene Symbol

Ltbp4

Ensembl Gene

ENSMUSG00000040488

Gene Name

latent transforming growth factor beta binding protein 4

Synonyms

2310046A13Rik

MMRRC Submission

041821-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4610 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

27004561-27037117 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 27006125 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Lysine at position 1453 (E1453K)

Ref Sequence

ENSEMBL: ENSMUSP00000113674 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000038618] [ENSMUST00000108369] [ENSMUST00000118583] [ENSMUST00000121175] [ENSMUST00000125455]

AlphaFold

Q8K4G1

Predicted Effect

probably damaging
Transcript: ENSMUST00000038618
AA Change: E1519K

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000037536
Gene: ENSMUSG00000040488
AA Change: E1519K

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
low complexity region	87	100	N/A	INTRINSIC
low complexity region	112	125	N/A	INTRINSIC
EGF	151	180	2.74e-3	SMART
low complexity region	244	265	N/A	INTRINSIC
EGF_CA	356	396	3.51e-10	SMART
Pfam:TB	416	457	4.8e-15	PFAM
low complexity region	490	501	N/A	INTRINSIC
low complexity region	507	568	N/A	INTRINSIC
low complexity region	579	590	N/A	INTRINSIC
EGF	591	629	6.06e-5	SMART
EGF_CA	630	671	8.3e-12	SMART
EGF_CA	672	713	7.34e-13	SMART
EGF_CA	714	751	8.43e-13	SMART
EGF_CA	753	794	1.66e-11	SMART
EGF_CA	795	836	3.61e-12	SMART
EGF_CA	837	876	5.61e-9	SMART
EGF_CA	877	919	1.73e-9	SMART
EGF_CA	920	961	7.12e-11	SMART
EGF_CA	962	1002	3.56e-11	SMART
EGF_CA	1003	1046	1.61e-9	SMART
EGF_CA	1047	1090	2.13e-9	SMART
EGF_CA	1091	1132	1.02e-11	SMART
EGF	1136	1175	1.69e1	SMART
low complexity region	1185	1223	N/A	INTRINSIC
Pfam:TB	1234	1276	1.7e-13	PFAM
EGF_CA	1295	1337	2.72e-7	SMART
EGF_CA	1338	1379	1.36e-7	SMART
Pfam:TB	1402	1443	4.3e-14	PFAM
low complexity region	1449	1461	N/A	INTRINSIC
low complexity region	1478	1500	N/A	INTRINSIC
EGF	1578	1615	6.06e-5	SMART
EGF_CA	1616	1660	9.54e-12	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000108369
AA Change: E1518K

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000104006
Gene: ENSMUSG00000040488
AA Change: E1518K

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
low complexity region	116	145	N/A	INTRINSIC
EGF	150	179	2.74e-3	SMART
low complexity region	243	264	N/A	INTRINSIC
EGF_CA	355	395	3.51e-10	SMART
Pfam:TB	414	456	2.5e-14	PFAM
low complexity region	489	500	N/A	INTRINSIC
low complexity region	506	567	N/A	INTRINSIC
low complexity region	578	589	N/A	INTRINSIC
EGF	590	628	6.06e-5	SMART
EGF_CA	629	670	8.3e-12	SMART
EGF_CA	671	712	7.34e-13	SMART
EGF_CA	713	750	8.43e-13	SMART
EGF_CA	752	793	1.66e-11	SMART
EGF_CA	794	835	3.61e-12	SMART
EGF_CA	836	875	5.61e-9	SMART
EGF_CA	876	918	1.73e-9	SMART
EGF_CA	919	960	7.12e-11	SMART
EGF_CA	961	1001	3.56e-11	SMART
EGF_CA	1002	1045	1.61e-9	SMART
EGF_CA	1046	1089	2.13e-9	SMART
EGF_CA	1090	1131	1.02e-11	SMART
EGF	1135	1174	1.69e1	SMART
low complexity region	1184	1222	N/A	INTRINSIC
Pfam:TB	1232	1275	2.4e-13	PFAM
EGF_CA	1294	1336	2.72e-7	SMART
EGF_CA	1337	1378	1.36e-7	SMART
Pfam:TB	1400	1442	7.6e-15	PFAM
low complexity region	1448	1460	N/A	INTRINSIC
low complexity region	1477	1499	N/A	INTRINSIC
EGF	1577	1614	6.06e-5	SMART
EGF_CA	1615	1659	9.54e-12	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000118583
AA Change: E1453K

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000113523
Gene: ENSMUSG00000040488
AA Change: E1453K

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
low complexity region	69	80	N/A	INTRINSIC
EGF	85	114	2.74e-3	SMART
low complexity region	178	199	N/A	INTRINSIC
EGF_CA	290	330	3.51e-10	SMART
Pfam:TB	349	391	2.3e-14	PFAM
low complexity region	424	435	N/A	INTRINSIC
low complexity region	441	502	N/A	INTRINSIC
low complexity region	513	524	N/A	INTRINSIC
EGF	525	563	6.06e-5	SMART
EGF_CA	564	605	8.3e-12	SMART
EGF_CA	606	647	7.34e-13	SMART
EGF_CA	648	685	8.43e-13	SMART
EGF_CA	687	728	1.66e-11	SMART
EGF_CA	729	770	3.61e-12	SMART
EGF_CA	771	810	5.61e-9	SMART
EGF_CA	811	853	1.73e-9	SMART
EGF_CA	854	895	7.12e-11	SMART
EGF_CA	896	936	3.56e-11	SMART
EGF_CA	937	980	1.61e-9	SMART
EGF_CA	981	1024	2.13e-9	SMART
EGF_CA	1025	1066	1.02e-11	SMART
EGF	1070	1109	1.69e1	SMART
low complexity region	1119	1157	N/A	INTRINSIC
Pfam:TB	1167	1210	2.2e-13	PFAM
EGF_CA	1229	1271	2.72e-7	SMART
EGF_CA	1272	1313	1.36e-7	SMART
Pfam:TB	1335	1377	7.1e-15	PFAM
low complexity region	1383	1395	N/A	INTRINSIC
low complexity region	1412	1434	N/A	INTRINSIC
EGF	1512	1549	6.06e-5	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000121175
AA Change: E1453K

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000113674
Gene: ENSMUSG00000040488
AA Change: E1453K

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
low complexity region	69	80	N/A	INTRINSIC
EGF	85	114	2.74e-3	SMART
low complexity region	178	199	N/A	INTRINSIC
EGF_CA	290	330	3.51e-10	SMART
Pfam:TB	349	391	3.4e-14	PFAM
low complexity region	424	435	N/A	INTRINSIC
low complexity region	441	502	N/A	INTRINSIC
low complexity region	513	524	N/A	INTRINSIC
EGF	525	563	6.06e-5	SMART
EGF_CA	564	605	8.3e-12	SMART
EGF_CA	606	647	7.34e-13	SMART
EGF_CA	648	685	8.43e-13	SMART
EGF_CA	687	728	1.66e-11	SMART
EGF_CA	729	770	3.61e-12	SMART
EGF_CA	771	810	5.61e-9	SMART
EGF_CA	811	853	1.73e-9	SMART
EGF_CA	854	895	7.12e-11	SMART
EGF_CA	896	936	3.56e-11	SMART
EGF_CA	937	980	1.61e-9	SMART
EGF_CA	981	1024	2.13e-9	SMART
EGF_CA	1025	1066	1.02e-11	SMART
EGF	1070	1109	1.69e1	SMART
low complexity region	1119	1157	N/A	INTRINSIC
Pfam:TB	1167	1210	3.3e-13	PFAM
EGF_CA	1229	1271	2.72e-7	SMART
EGF_CA	1272	1313	1.36e-7	SMART
Pfam:TB	1335	1377	1e-14	PFAM
low complexity region	1383	1395	N/A	INTRINSIC
low complexity region	1412	1434	N/A	INTRINSIC
EGF	1512	1549	6.06e-5	SMART
EGF_CA	1550	1594	9.54e-12	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000125455
AA Change: E203K

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000120326
Gene: ENSMUSG00000040488
AA Change: E203K

Domain	Start	End	E-Value	Type
Pfam:TB	9	52	1.9e-14	PFAM
Pfam:TB	85	127	6e-16	PFAM
low complexity region	133	145	N/A	INTRINSIC
low complexity region	162	184	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000129471

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000134464

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000148594

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000206413

Meta Mutation Damage Score

0.1659

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.5%

Validation Efficiency

98% (121/124)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene binds transforming growth factor beta (TGFB) as it is secreted and targeted to the extracellular matrix. TGFB is biologically latent after secretion and insertion into the extracellular matrix, and sheds TGFB and other proteins upon activation. Defects in this gene may be a cause of cutis laxa and severe pulmonary, gastrointestinal, and urinary abnormalities. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]
PHENOTYPE: Homozygous disruption of this locus results in pulmonary emphysema, cardiomyopathy, and colon cancer. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 117 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aars2	T	A	17: 45,827,847 (GRCm39)	D555E	probably damaging	Het
Adgre1	C	A	17: 57,757,073 (GRCm39)	Q777K	possibly damaging	Het
Agpat4	G	A	17: 12,429,264 (GRCm39)		probably null	Het
Ak7	G	A	12: 105,679,834 (GRCm39)	V123M	probably benign	Het
Ankle1	AT	A	8: 71,859,851 (GRCm39)		probably benign	Het
Ankrd44	T	G	1: 54,805,907 (GRCm39)		probably benign	Het
Aprt	A	T	8: 123,302,154 (GRCm39)		probably null	Het
Aptx	T	C	4: 40,702,766 (GRCm39)		probably null	Het
Arsi	G	A	18: 61,049,723 (GRCm39)	G202E	probably benign	Het
AY358078	T	A	14: 52,063,532 (GRCm39)	C393S	possibly damaging	Het
Bbip1	T	C	19: 53,920,606 (GRCm39)	M1V	probably null	Het
Cacng7	T	A	7: 3,385,207 (GRCm39)	M36K	probably benign	Het
Camta1	A	G	4: 151,169,284 (GRCm39)	W156R	probably damaging	Het
Casd1	G	A	6: 4,631,165 (GRCm39)		probably null	Het
Casz1	T	A	4: 149,017,724 (GRCm39)	Y338N	probably damaging	Het
Ccdc66	T	C	14: 27,222,377 (GRCm39)	N122S	probably damaging	Het
Celf2	G	T	2: 6,590,831 (GRCm39)	N279K	possibly damaging	Het
Cfap96	A	T	8: 46,423,505 (GRCm39)	I69N	probably damaging	Het
Cit	A	G	5: 116,132,146 (GRCm39)	T1801A	probably benign	Het
Cnot2	A	G	10: 116,335,323 (GRCm39)	I275T	probably damaging	Het
Ddx4	T	C	13: 112,748,594 (GRCm39)	K435E	probably damaging	Het
Dnajc6	T	C	4: 101,468,461 (GRCm39)	F166L	probably damaging	Het
Dst	T	C	1: 34,208,937 (GRCm39)	L820P	probably damaging	Het
Dusp11	T	A	6: 85,927,037 (GRCm39)	N193Y	probably damaging	Het
Eif1ad7	T	C	12: 88,238,544 (GRCm39)	N72S	probably benign	Het
Eif3m	A	T	2: 104,843,633 (GRCm39)	N116K	probably benign	Het
Epb41l1	A	T	2: 156,351,181 (GRCm39)	E418D	possibly damaging	Het
Esyt2	T	G	12: 116,282,510 (GRCm39)	N153K	probably damaging	Het
Exoc6b	T	G	6: 84,980,141 (GRCm39)		probably benign	Het
Ezr	C	T	17: 7,007,121 (GRCm39)	E502K	possibly damaging	Het
Fbxw11	T	A	11: 32,661,859 (GRCm39)	Y66N	possibly damaging	Het
Frem2	A	G	3: 53,455,228 (GRCm39)	L2116S	possibly damaging	Het
Fry	T	C	5: 150,309,569 (GRCm39)	L671P	probably damaging	Het
Galnt7	A	G	8: 57,998,803 (GRCm39)	I262T	probably damaging	Het
Glp1r	T	C	17: 31,150,221 (GRCm39)	F381S	probably benign	Het
Gm3867	T	C	9: 36,168,567 (GRCm39)		noncoding transcript	Het
Gm8741	G	T	17: 35,555,062 (GRCm39)		noncoding transcript	Het
Golgb1	A	G	16: 36,738,987 (GRCm39)	D2442G	probably damaging	Het
Gp1bb	A	T	16: 18,439,893 (GRCm39)	L67Q	probably damaging	Het
Gstm7	G	A	3: 107,834,235 (GRCm39)	T206I	possibly damaging	Het
H2bc7	A	T	13: 23,758,231 (GRCm39)	V45E	possibly damaging	Het
Hs3st5	A	T	10: 36,704,802 (GRCm39)	D35V	probably benign	Het
Hspa13	T	C	16: 75,558,190 (GRCm39)	H125R	probably benign	Het
Hspa1a	T	G	17: 35,190,156 (GRCm39)	H249P	probably damaging	Het
Igkv10-95	A	T	6: 68,657,562 (GRCm39)	Q6L	probably damaging	Het
Il1rap	T	A	16: 26,533,526 (GRCm39)	L474H	probably benign	Het
Ipo11	T	A	13: 107,016,245 (GRCm39)	Y489F	probably benign	Het
Itga5	A	G	15: 103,259,259 (GRCm39)	Y723H	probably damaging	Het
Itih2	T	C	2: 10,109,971 (GRCm39)	N594S	probably damaging	Het
Itk	T	A	11: 46,227,342 (GRCm39)	Q427L	probably benign	Het
Kif26b	A	G	1: 178,506,920 (GRCm39)	Y332C	probably damaging	Het
Kmt2c	G	A	5: 25,559,382 (GRCm39)	R1086W	probably damaging	Het
Ktn1	T	A	14: 47,963,636 (GRCm39)		probably benign	Het
Lars2	T	A	9: 123,247,758 (GRCm39)	I305N	probably damaging	Het
Lgmn	G	T	12: 102,366,383 (GRCm39)		probably benign	Het
Lypd8	G	A	11: 58,277,675 (GRCm39)	M152I	probably benign	Het
Man2a1	T	A	17: 65,019,454 (GRCm39)	S773T	probably benign	Het
Map2k6	T	G	11: 110,390,300 (GRCm39)	L278R	probably damaging	Het
Mbtps1	A	T	8: 120,262,086 (GRCm39)	D354E	probably damaging	Het
Mcpt9	C	T	14: 56,266,049 (GRCm39)	V60M	probably damaging	Het
Mical3	C	A	6: 120,911,799 (GRCm39)	E1083*	probably null	Het
Mms19	A	G	19: 41,933,935 (GRCm39)	V811A	possibly damaging	Het
Mrc2	G	A	11: 105,239,257 (GRCm39)		probably null	Het
Mslnl	G	A	17: 25,961,908 (GRCm39)	V128M	probably damaging	Het
Mtcl1	T	A	17: 66,684,882 (GRCm39)	H520L	probably benign	Het
Mymk	A	T	2: 26,952,719 (GRCm39)	F130I	probably damaging	Het
Myo1f	C	T	17: 33,801,306 (GRCm39)	R333C	probably damaging	Het
Myo9a	T	A	9: 59,779,165 (GRCm39)	H1640Q	probably benign	Het
Nav1	A	G	1: 135,520,186 (GRCm39)		probably benign	Het
Ncbp3	G	T	11: 72,969,844 (GRCm39)	G564C	probably damaging	Het
Ncoa4	A	T	14: 31,898,682 (GRCm39)	I501L	probably benign	Het
Ngp	T	A	9: 110,249,883 (GRCm39)	N60K	possibly damaging	Het
Npc1l1	G	T	11: 6,178,215 (GRCm39)	D398E	probably damaging	Het
Nphs2	T	A	1: 156,153,701 (GRCm39)	M264K	probably damaging	Het
Or4s2b	G	A	2: 88,508,240 (GRCm39)	V14I	probably benign	Het
Or4s2b	T	G	2: 88,508,523 (GRCm39)	V101G	probably benign	Het
Or7e165	T	A	9: 19,694,442 (GRCm39)	Y4*	probably null	Het
Or8b8	T	A	9: 37,809,622 (GRCm39)	S307R	probably benign	Het
Patz1	A	G	11: 3,256,241 (GRCm39)	Y509C	probably damaging	Het
Pax8	G	A	2: 24,311,595 (GRCm39)	P447S	probably damaging	Het
Pde11a	A	G	2: 75,988,677 (GRCm39)	V488A	probably benign	Het
Pex11g	C	T	8: 3,515,899 (GRCm39)	V45M	probably benign	Het
Pik3ip1	T	A	11: 3,283,327 (GRCm39)	S142R	probably damaging	Het
Pitpnm2	C	G	5: 124,263,434 (GRCm39)	A819P	probably damaging	Het
Pla2g4e	T	G	2: 120,016,863 (GRCm39)	H226P	possibly damaging	Het
Plin4	T	A	17: 56,412,418 (GRCm39)	M538L	probably benign	Het
Ppp3cb	T	C	14: 20,570,714 (GRCm39)	N339S	possibly damaging	Het
Rev1	T	C	1: 38,092,730 (GRCm39)	E1202G	probably damaging	Het
Rngtt	T	A	4: 33,339,133 (GRCm39)		probably benign	Het
Serpinb12	T	A	1: 106,876,883 (GRCm39)	D66E	probably benign	Het
Sgsm1	A	T	5: 113,403,173 (GRCm39)	F958Y	probably damaging	Het
Slc35e2	T	C	4: 155,702,106 (GRCm39)	F290S	probably benign	Het
Sorl1	C	T	9: 41,943,210 (GRCm39)	V889M	possibly damaging	Het
Sptlc3	G	A	2: 139,478,600 (GRCm39)	V520I	probably benign	Het
Stam	A	T	2: 14,120,669 (GRCm39)	H53L	probably damaging	Het
Stox2	A	G	8: 47,645,970 (GRCm39)	S497P	probably damaging	Het
Tarbp1	A	G	8: 127,201,069 (GRCm39)	Y246H	probably damaging	Het
Tbx15	A	G	3: 99,259,683 (GRCm39)	Y518C	probably damaging	Het
Tdrd5	T	A	1: 156,111,944 (GRCm39)	T479S	probably benign	Het
Tescl	T	C	7: 24,032,683 (GRCm39)	E214G	probably damaging	Het
Tex10	T	C	4: 48,452,946 (GRCm39)	D671G	probably benign	Het
Tmem132d	A	G	5: 128,061,360 (GRCm39)	V414A	probably benign	Het
Tmem41b	T	A	7: 109,573,941 (GRCm39)		probably benign	Het
Tnfrsf18	A	G	4: 156,106,337 (GRCm39)		probably benign	Het
Tulp4	T	A	17: 6,249,108 (GRCm39)	D42E	probably damaging	Het
Ubtd1	A	G	19: 42,022,103 (GRCm39)	N125S	probably damaging	Het
Ubxn4	T	A	1: 128,183,186 (GRCm39)	F68I	probably benign	Het
Urb1	A	G	16: 90,573,159 (GRCm39)	S958P	probably benign	Het
Vash2	T	C	1: 190,692,498 (GRCm39)	S226G	probably benign	Het
Vmn2r120	C	T	17: 57,816,120 (GRCm39)	G745E	probably damaging	Het
Vmn2r58	T	A	7: 41,487,117 (GRCm39)	I593F	probably benign	Het
Zfp398	T	C	6: 47,817,361 (GRCm39)	L67P	probably damaging	Het
Zfp607b	T	A	7: 27,403,120 (GRCm39)	H525Q	probably damaging	Het
Zfp629	T	C	7: 127,211,492 (GRCm39)	T106A	probably benign	Het
Zfp980	G	A	4: 145,428,653 (GRCm39)	G461S	probably benign	Het
Zic5	T	A	14: 122,702,212 (GRCm39)	D173V	probably damaging	Het
Zranb2	G	A	3: 157,247,521 (GRCm39)		probably benign	Het

Other mutations in Ltbp4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00434:Ltbp4	APN	7	27,028,230 (GRCm39)	missense	probably damaging	0.96
IGL00585:Ltbp4	APN	7	27,026,158 (GRCm39)	missense	probably damaging	0.98
IGL01320:Ltbp4	APN	7	27,027,784 (GRCm39)	splice site	probably benign
IGL01860:Ltbp4	APN	7	27,019,071 (GRCm39)	missense	probably damaging	1.00
IGL02026:Ltbp4	APN	7	27,026,842 (GRCm39)	nonsense	probably null
IGL02226:Ltbp4	APN	7	27,006,359 (GRCm39)	missense	probably damaging	1.00
IGL02422:Ltbp4	APN	7	27,019,097 (GRCm39)	missense	probably damaging	1.00
IGL02611:Ltbp4	APN	7	27,010,080 (GRCm39)	missense	probably damaging	1.00
IGL02892:Ltbp4	APN	7	27,010,074 (GRCm39)	missense	probably damaging	1.00
IGL02926:Ltbp4	APN	7	27,028,297 (GRCm39)	splice site	probably null
IGL02950:Ltbp4	APN	7	27,006,143 (GRCm39)	missense	probably damaging	0.99
IGL03008:Ltbp4	APN	7	27,023,789 (GRCm39)	missense	probably damaging	0.99
IGL03271:Ltbp4	APN	7	27,029,240 (GRCm39)	missense	unknown
IGL02837:Ltbp4	UTSW	7	27,013,806 (GRCm39)	missense	probably damaging	1.00
R0792:Ltbp4	UTSW	7	27,024,485 (GRCm39)	missense	probably damaging	1.00
R0980:Ltbp4	UTSW	7	27,023,587 (GRCm39)	missense	probably damaging	1.00
R1017:Ltbp4	UTSW	7	27,005,501 (GRCm39)	missense	possibly damaging	0.95
R1185:Ltbp4	UTSW	7	27,009,960 (GRCm39)	missense	probably damaging	1.00
R1185:Ltbp4	UTSW	7	27,009,960 (GRCm39)	missense	probably damaging	1.00
R1185:Ltbp4	UTSW	7	27,009,960 (GRCm39)	missense	probably damaging	1.00
R1403:Ltbp4	UTSW	7	27,028,464 (GRCm39)	missense	unknown
R1403:Ltbp4	UTSW	7	27,028,464 (GRCm39)	missense	unknown
R1448:Ltbp4	UTSW	7	27,006,002 (GRCm39)	missense	possibly damaging	0.86
R1575:Ltbp4	UTSW	7	27,022,245 (GRCm39)	missense	probably damaging	1.00
R1918:Ltbp4	UTSW	7	27,036,994 (GRCm39)	unclassified	probably benign
R1932:Ltbp4	UTSW	7	27,007,191 (GRCm39)	critical splice donor site	probably null
R1959:Ltbp4	UTSW	7	27,028,443 (GRCm39)	missense	unknown
R1960:Ltbp4	UTSW	7	27,028,443 (GRCm39)	missense	unknown
R1976:Ltbp4	UTSW	7	27,026,195 (GRCm39)	missense	probably damaging	1.00
R2060:Ltbp4	UTSW	7	27,008,378 (GRCm39)	missense	probably damaging	1.00
R2333:Ltbp4	UTSW	7	27,027,203 (GRCm39)	missense	possibly damaging	0.82
R2431:Ltbp4	UTSW	7	27,019,101 (GRCm39)	missense	possibly damaging	0.91
R3125:Ltbp4	UTSW	7	27,027,203 (GRCm39)	missense	possibly damaging	0.82
R4093:Ltbp4	UTSW	7	27,024,641 (GRCm39)	missense	possibly damaging	0.93
R4095:Ltbp4	UTSW	7	27,024,641 (GRCm39)	missense	possibly damaging	0.93
R4592:Ltbp4	UTSW	7	27,024,608 (GRCm39)	missense	probably damaging	0.96
R4650:Ltbp4	UTSW	7	27,013,734 (GRCm39)	missense	probably damaging	1.00
R4912:Ltbp4	UTSW	7	27,005,541 (GRCm39)	nonsense	probably null
R5002:Ltbp4	UTSW	7	27,027,110 (GRCm39)	frame shift	probably null
R5016:Ltbp4	UTSW	7	27,027,110 (GRCm39)	frame shift	probably null
R5216:Ltbp4	UTSW	7	27,026,736 (GRCm39)	small deletion	probably benign
R5218:Ltbp4	UTSW	7	27,026,736 (GRCm39)	small deletion	probably benign
R5219:Ltbp4	UTSW	7	27,026,746 (GRCm39)	missense	probably benign	0.01
R5219:Ltbp4	UTSW	7	27,026,736 (GRCm39)	small deletion	probably benign
R5539:Ltbp4	UTSW	7	27,027,149 (GRCm39)	missense	probably damaging	0.97
R5991:Ltbp4	UTSW	7	27,008,741 (GRCm39)	missense	probably damaging	1.00
R6082:Ltbp4	UTSW	7	27,035,105 (GRCm39)	unclassified	probably benign
R6125:Ltbp4	UTSW	7	27,027,180 (GRCm39)	missense	probably damaging	1.00
R6146:Ltbp4	UTSW	7	27,019,149 (GRCm39)	missense	probably damaging	0.99
R6156:Ltbp4	UTSW	7	27,029,587 (GRCm39)	missense	unknown
R6414:Ltbp4	UTSW	7	27,010,140 (GRCm39)	missense	probably damaging	1.00
R6563:Ltbp4	UTSW	7	27,008,488 (GRCm39)	missense	probably damaging	1.00
R6719:Ltbp4	UTSW	7	27,028,188 (GRCm39)	missense	probably damaging	0.99
R6940:Ltbp4	UTSW	7	27,008,369 (GRCm39)	missense	probably damaging	1.00
R7054:Ltbp4	UTSW	7	27,007,191 (GRCm39)	critical splice donor site	probably null
R7116:Ltbp4	UTSW	7	27,004,852 (GRCm39)	missense	probably damaging	0.99
R7326:Ltbp4	UTSW	7	27,029,180 (GRCm39)	missense	unknown
R7419:Ltbp4	UTSW	7	27,029,192 (GRCm39)	missense	unknown
R8068:Ltbp4	UTSW	7	27,023,593 (GRCm39)	missense	probably damaging	0.99
R8435:Ltbp4	UTSW	7	27,034,870 (GRCm39)	missense	unknown
R8543:Ltbp4	UTSW	7	27,024,666 (GRCm39)	missense	possibly damaging	0.84
R8897:Ltbp4	UTSW	7	27,026,119 (GRCm39)	missense	probably benign	0.09
R9103:Ltbp4	UTSW	7	27,028,186 (GRCm39)	missense	unknown
R9131:Ltbp4	UTSW	7	27,036,976 (GRCm39)	missense	unknown
R9206:Ltbp4	UTSW	7	27,022,350 (GRCm39)	missense	probably damaging	1.00
R9301:Ltbp4	UTSW	7	27,021,578 (GRCm39)	missense	probably damaging	0.98
R9302:Ltbp4	UTSW	7	27,008,481 (GRCm39)	missense	possibly damaging	0.80
X0025:Ltbp4	UTSW	7	27,025,227 (GRCm39)	missense	probably damaging	1.00
X0066:Ltbp4	UTSW	7	27,005,490 (GRCm39)	critical splice donor site	probably null
Z1088:Ltbp4	UTSW	7	27,007,217 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGCATCTCCCATCTTGTGTG -3'
(R):5'- CTGGTGGCTTTGGAATTCCC -3'

Sequencing Primer
(F):5'- CATCTCCCATCTTGTGTGTGTGTG -3'
(R):5'- GGAATTCCCTATGAATATGGCCCAG -3'

Posted On

2015-09-25