Mutagenetix > Incidental Mutation

Incidental Mutation 'R4599:Vmn2r13'

345429

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r13

Ensembl Gene

ENSMUSG00000091635

Gene Name

vomeronasal 2, receptor 13

Synonyms

Gm4867

MMRRC Submission

041815-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.086) question?

Stock #

R4599 (G1)

Quality Score

216

Status

Not validated

Chromosome

Chromosomal Location

109156068-109192107 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 109156456 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Asparagine at position 703 (I703N)

Ref Sequence

ENSEMBL: ENSMUSP00000052977 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053253]

AlphaFold

L7N1X2

Predicted Effect

probably damaging
Transcript: ENSMUST00000053253
AA Change: I703N

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000052977
Gene: ENSMUSG00000091635
AA Change: I703N

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	76	463	2.8e-29	PFAM
Pfam:NCD3G	506	560	1.3e-18	PFAM
Pfam:7tm_3	593	828	1.8e-54	PFAM

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 69 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca14	T	C	7: 120,255,403	V930A	probably benign	Het
Ankrd13b	T	C	11: 77,471,668	R677G	probably benign	Het
Apc	T	A	18: 34,317,987	Y2611*	probably null	Het
Apold1	A	G	6: 134,984,069	Y162C	probably damaging	Het
Atp6v0a4	T	C	6: 38,078,802	I325V	probably benign	Het
Cab39	A	G	1: 85,848,329	Y249C	probably damaging	Het
Cd22	T	A	7: 30,875,900	H239L	probably damaging	Het
Chrna7	A	G	7: 63,103,790	M327T	probably damaging	Het
Clock	T	C	5: 76,235,810	M499V	probably benign	Het
Clspn	A	G	4: 126,581,460	E1002G	probably benign	Het
Clta	C	T	4: 44,012,819	P10S	probably damaging	Het
Col5a3	A	G	9: 20,774,559		probably null	Het
Coq6	T	C	12: 84,362,139	V30A	probably benign	Het
Csmd2	G	T	4: 127,988,128	R20L	probably benign	Het
D430041D05Rik	A	T	2: 104,208,183	V1547D	probably damaging	Het
Dapk1	C	T	13: 60,718,047	P153S	probably benign	Het
Dock2	T	A	11: 34,239,536	Y1545F	probably damaging	Het
Dpp6	G	T	5: 27,634,548	G354C	probably damaging	Het
Dyrk1b	T	C	7: 28,182,431	L105P	probably damaging	Het
Epor	A	G	9: 21,961,859	S86P	probably benign	Het
Fam166b	T	C	4: 43,427,574	H250R	possibly damaging	Het
Gale	C	A	4: 135,967,837	S341*	probably null	Het
Galnt4	T	A	10: 99,109,493	V360E	probably damaging	Het
Gart	A	T	16: 91,622,945	C24*	probably null	Het
Gcnt2	G	T	13: 40,887,490	V42L	probably benign	Het
Herc6	A	G	6: 57,659,713	I805V	probably benign	Het
Ints12	T	A	3: 133,098,453	I67N	probably benign	Het
Irx1	C	A	13: 71,960,113	R150L	probably damaging	Het
Kif26b	A	G	1: 178,530,459	Y45C	unknown	Het
Krt35	T	C	11: 100,094,008	T275A	probably damaging	Het
Laptm5	G	T	4: 130,916,005		probably benign	Het
Lin7a	T	C	10: 107,412,166	S111P	unknown	Het
Med27	A	G	2: 29,524,458	D159G	probably damaging	Het
Msh2	A	G	17: 87,708,578	K546R	probably damaging	Het
Myo1a	A	T	10: 127,720,151		probably null	Het
Myo1c	T	C	11: 75,668,193	F604L	probably damaging	Het
Myrip	A	G	9: 120,464,784	K782E	probably damaging	Het
Ndc80	T	C	17: 71,521,068	D88G	probably damaging	Het
Nrxn2	A	G	19: 6,455,252	D375G	probably damaging	Het
Olfr1380	T	C	11: 49,564,718	S266P	probably damaging	Het
Olfr676	A	G	7: 105,036,073	I292V	probably benign	Het
Padi3	T	C	4: 140,798,111	H187R	probably damaging	Het
Pcdhgb1	A	T	18: 37,681,557	N367I	probably damaging	Het
Pdrg1	T	C	2: 153,012,390	I77V	probably benign	Het
Pfas	T	C	11: 68,991,069	E930G	probably benign	Het
Pik3cb	C	T	9: 99,061,764	R662Q	probably benign	Het
Pla2g4e	T	G	2: 120,186,382	H226P	possibly damaging	Het
Plxnd1	A	T	6: 115,994,276	V177E	probably damaging	Het
Prmt7	A	G	8: 106,250,329	S558G	possibly damaging	Het
Pspc1	A	C	14: 56,777,789		probably null	Het
Rilp	T	C	11: 75,512,760	S343P	probably benign	Het
Ror1	A	G	4: 100,407,910	M194V	probably damaging	Het
Rsu1	T	C	2: 13,170,004	Y225C	probably damaging	Het
Rundc1	A	G	11: 101,433,926	N486S	probably damaging	Het
Sema6d	T	A	2: 124,654,231	I65N	probably damaging	Het
Slc5a11	A	G	7: 123,258,378	E230G	probably benign	Het
Spint1	T	C	2: 119,246,460	S342P	probably damaging	Het
Stard3nl	A	G	13: 19,367,753	S214P	probably damaging	Het
Tcp10a	A	C	17: 7,336,924	T271P	probably damaging	Het
Tie1	A	G	4: 118,472,634	Y1091H	probably benign	Het
Tlr12	T	C	4: 128,617,332	Y375C	probably benign	Het
Tmem107	T	A	11: 69,071,448	M77K	probably damaging	Het
Tns2	C	T	15: 102,108,934	R281C	probably damaging	Het
Tns3	T	C	11: 8,531,747	K202E	probably damaging	Het
Tspoap1	C	T	11: 87,779,521	P1634L	probably damaging	Het
Ttc7b	G	A	12: 100,500,117	R79C	probably damaging	Het
Ush2a	A	G	1: 188,911,647	N4402S	probably benign	Het
Xrcc4	A	G	13: 90,062,007		probably null	Het
Zp3	A	T	5: 135,984,235	K168*	probably null	Het

Other mutations in Vmn2r13

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00965:Vmn2r13	APN	5	109156098	missense	probably damaging	1.00
IGL01373:Vmn2r13	APN	5	109156702	missense	probably damaging	1.00
IGL01946:Vmn2r13	APN	5	109174219	missense	probably benign	0.01
IGL01971:Vmn2r13	APN	5	109174115	missense	probably benign	0.01
IGL02636:Vmn2r13	APN	5	109192017	missense	probably damaging	0.98
IGL03062:Vmn2r13	APN	5	109156282	missense	probably damaging	1.00
IGL03173:Vmn2r13	APN	5	109171779	missense	possibly damaging	0.95
IGL03301:Vmn2r13	APN	5	109158089	missense	probably damaging	0.99
IGL03383:Vmn2r13	APN	5	109156532	missense	probably damaging	0.98
IGL03048:Vmn2r13	UTSW	5	109156285	missense	probably damaging	1.00
R0123:Vmn2r13	UTSW	5	109175049	missense	probably benign	0.00
R0134:Vmn2r13	UTSW	5	109175049	missense	probably benign	0.00
R0220:Vmn2r13	UTSW	5	109156466	missense	probably damaging	1.00
R0225:Vmn2r13	UTSW	5	109175049	missense	probably benign	0.00
R0393:Vmn2r13	UTSW	5	109156529	missense	probably benign	0.01
R0410:Vmn2r13	UTSW	5	109173813	missense	probably benign	0.35
R0787:Vmn2r13	UTSW	5	109156847	missense	probably damaging	0.99
R1200:Vmn2r13	UTSW	5	109174202	missense	probably damaging	1.00
R1448:Vmn2r13	UTSW	5	109174135	missense	probably damaging	1.00
R1782:Vmn2r13	UTSW	5	109158174	missense	probably benign	0.08
R1939:Vmn2r13	UTSW	5	109191986	missense	possibly damaging	0.88
R2029:Vmn2r13	UTSW	5	109192077	missense	probably benign	0.13
R2125:Vmn2r13	UTSW	5	109158192	missense	probably benign	0.00
R2126:Vmn2r13	UTSW	5	109158192	missense	probably benign	0.00
R2379:Vmn2r13	UTSW	5	109171778	missense	probably benign	0.05
R2680:Vmn2r13	UTSW	5	109174312	missense	possibly damaging	0.66
R2888:Vmn2r13	UTSW	5	109191974	missense	possibly damaging	0.88
R2889:Vmn2r13	UTSW	5	109191974	missense	possibly damaging	0.88
R2890:Vmn2r13	UTSW	5	109191974	missense	possibly damaging	0.88
R3014:Vmn2r13	UTSW	5	109171761	missense	possibly damaging	0.81
R3683:Vmn2r13	UTSW	5	109156855	missense	probably damaging	1.00
R4074:Vmn2r13	UTSW	5	109156700	missense	probably damaging	1.00
R4614:Vmn2r13	UTSW	5	109175199	missense	probably benign	0.01
R4805:Vmn2r13	UTSW	5	109156465	missense	probably damaging	1.00
R4822:Vmn2r13	UTSW	5	109174072	missense	probably damaging	0.99
R4943:Vmn2r13	UTSW	5	109175049	missense	probably benign	0.00
R5263:Vmn2r13	UTSW	5	109173975	missense	probably benign	0.00
R5297:Vmn2r13	UTSW	5	109191939	missense	probably benign	0.00
R5502:Vmn2r13	UTSW	5	109173714	missense	probably damaging	1.00
R5554:Vmn2r13	UTSW	5	109191994	missense	possibly damaging	0.49
R5563:Vmn2r13	UTSW	5	109173980	missense	probably benign	0.00
R5819:Vmn2r13	UTSW	5	109174100	missense	possibly damaging	0.79
R6074:Vmn2r13	UTSW	5	109174301	missense	probably benign	0.04
R6416:Vmn2r13	UTSW	5	109174116	missense	probably damaging	0.99
R6419:Vmn2r13	UTSW	5	109175219	missense	possibly damaging	0.87
R6484:Vmn2r13	UTSW	5	109156674	nonsense	probably null
R6486:Vmn2r13	UTSW	5	109156559	missense	probably benign	0.05
R6545:Vmn2r13	UTSW	5	109156940	splice site	probably null
R6700:Vmn2r13	UTSW	5	109175072	missense	probably benign	0.00
R6897:Vmn2r13	UTSW	5	109158149	missense	possibly damaging	0.90
R6957:Vmn2r13	UTSW	5	109156887	nonsense	probably null
R7276:Vmn2r13	UTSW	5	109173779	missense	probably damaging	1.00
R7363:Vmn2r13	UTSW	5	109192043	missense	probably benign	0.03
R7443:Vmn2r13	UTSW	5	109192043	missense	probably benign	0.03
R7555:Vmn2r13	UTSW	5	109171691	splice site	probably null
R7607:Vmn2r13	UTSW	5	109173640	missense	probably damaging	0.98
R7719:Vmn2r13	UTSW	5	109171752	missense	probably benign	0.00
R8116:Vmn2r13	UTSW	5	109175060	missense	probably benign	0.12
R8242:Vmn2r13	UTSW	5	109175006	missense	possibly damaging	0.65
R8294:Vmn2r13	UTSW	5	109175112	missense	probably benign	0.02
R8340:Vmn2r13	UTSW	5	109174140	missense	probably benign	0.00
R8692:Vmn2r13	UTSW	5	109171648	missense	probably benign	0.03
R8742:Vmn2r13	UTSW	5	109156397	missense	probably benign	0.02
R9022:Vmn2r13	UTSW	5	109156376	missense	possibly damaging	0.94
R9281:Vmn2r13	UTSW	5	109156087	missense	probably damaging	1.00
R9529:Vmn2r13	UTSW	5	109156198	missense	probably damaging	1.00
R9708:Vmn2r13	UTSW	5	109174141	missense	probably benign	0.00
R9746:Vmn2r13	UTSW	5	109191907	critical splice donor site	probably null
X0066:Vmn2r13	UTSW	5	109156219	missense	probably benign	0.44

Predicted Primers

PCR Primer
(F):5'- AGCATGCTGAAGGTTAGGAATTTG -3'
(R):5'- CCTGCAGCAGATCACATTTGG -3'

Sequencing Primer
(F):5'- GCTTCATTGAATCTGTCAGGAAG -3'
(R):5'- CACATTTGGAGTATTTTTCACAGTGG -3'

Posted On

2015-09-25