Mutagenetix > Incidental Mutation

Incidental Mutation 'R4601:Mslnl'

345669

Institutional Source

Beutler Lab

Gene Symbol

Mslnl

Ensembl Gene

ENSMUSG00000041062

Gene Name

mesothelin-like

Synonyms

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4601 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

25736040-25748330 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 25742934 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Methionine at position 128 (V128M)

Ref Sequence

ENSEMBL: ENSMUSP00000049020 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047098]

AlphaFold

Q8C160

Predicted Effect

probably damaging
Transcript: ENSMUST00000047098
AA Change: V128M

PolyPhen 2 Score 0.971 (Sensitivity: 0.77; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000049020
Gene: ENSMUSG00000041062
AA Change: V128M

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:Mesothelin	29	589	2.8e-70	PFAM
low complexity region	633	653	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000102319

Meta Mutation Damage Score

0.2148

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.1%
20x: 94.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 91 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700029J07Rik	A	T	8: 45,970,468 (GRCm38)	I69N	probably damaging	Het
4930435E12Rik	A	G	16: 38,828,018 (GRCm38)	V243A	probably benign	Het
Aars2	T	A	17: 45,516,921 (GRCm38)	D555E	probably damaging	Het
Abca16	T	G	7: 120,436,697 (GRCm38)	F334L	probably damaging	Het
Abcc4	T	A	14: 118,632,163 (GRCm38)	M186L	probably benign	Het
Agap3	T	C	5: 24,476,408 (GRCm38)	L120P	probably damaging	Het
Ak7	G	A	12: 105,713,575 (GRCm38)	V123M	probably benign	Het
Aldh1a7	A	T	19: 20,715,979 (GRCm38)	V192D	probably damaging	Het
Ankrd36	A	G	11: 5,570,102 (GRCm38)	D59G	probably benign	Het
Arsi	G	A	18: 60,916,651 (GRCm38)	G202E	probably benign	Het
Cacna1e	A	G	1: 154,471,613 (GRCm38)	V936A	probably benign	Het
Camkv	T	C	9: 107,946,096 (GRCm38)	V107A	probably damaging	Het
Camp	T	C	9: 109,848,662 (GRCm38)	E80G	probably damaging	Het
Ccdc66	T	C	14: 27,500,420 (GRCm38)	N122S	probably damaging	Het
Cd101	G	A	3: 100,993,888 (GRCm38)	T960M	possibly damaging	Het
Cdk18	A	G	1: 132,116,919 (GRCm38)	V323A	possibly damaging	Het
Celf2	G	T	2: 6,586,020 (GRCm38)	N279K	possibly damaging	Het
Cemip	T	C	7: 83,951,618 (GRCm38)	I932V	probably damaging	Het
Cep250	C	A	2: 155,962,053 (GRCm38)	Q28K	probably benign	Het
Ces2f	T	C	8: 104,949,964 (GRCm38)	C97R	probably damaging	Het
Cfap126	G	T	1: 171,114,058 (GRCm38)	G41C	possibly damaging	Het
Chat	T	C	14: 32,424,155 (GRCm38)	M354V	probably benign	Het
Clca4b	A	T	3: 144,927,184 (GRCm38)	D168E	possibly damaging	Het
Cpxm1	T	A	2: 130,393,576 (GRCm38)	M499L	possibly damaging	Het
Dennd3	T	A	15: 73,567,160 (GRCm38)	W1126R	probably damaging	Het
Dgkb	T	C	12: 38,602,820 (GRCm38)	S735P	probably damaging	Het
Dlec1	T	C	9: 119,147,134 (GRCm38)		probably null	Het
Dnajc6	T	C	4: 101,611,264 (GRCm38)	F166L	probably damaging	Het
Dph5	A	G	3: 115,899,777 (GRCm38)	N115D	possibly damaging	Het
Ercc3	G	A	18: 32,245,571 (GRCm38)	A202T	probably benign	Het
Erich3	A	T	3: 154,764,738 (GRCm38)	D136V	unknown	Het
Exoc2	T	C	13: 30,882,268 (GRCm38)	N475S	probably benign	Het
Fam160a1	T	C	3: 85,741,180 (GRCm38)	M26V	probably damaging	Het
Fam217a	T	C	13: 34,911,302 (GRCm38)	D310G	probably damaging	Het
Fam53a	C	T	5: 33,600,663 (GRCm38)	S372N	probably benign	Het
Fbn2	C	G	18: 58,053,733 (GRCm38)	G1699R	probably damaging	Het
Fbxo46	T	C	7: 19,135,564 (GRCm38)	V36A	probably benign	Het
G6pc	A	G	11: 101,372,741 (GRCm38)	Y127C	probably damaging	Het
Gba2	A	G	4: 43,573,810 (GRCm38)	F161L	probably damaging	Het
Gif	A	C	19: 11,752,190 (GRCm38)	D171A	probably damaging	Het
Gja1	T	C	10: 56,388,229 (GRCm38)	L228P	probably damaging	Het
Gm20834	A	G	Y: 10,323,178 (GRCm38)	V86A	probably benign	Het
Hmcn1	A	T	1: 150,738,645 (GRCm38)	C1337S	probably damaging	Het
Il1rl1	T	A	1: 40,441,300 (GRCm38)	S30T	possibly damaging	Het
Itgb4	A	G	11: 116,005,722 (GRCm38)	T1436A	probably damaging	Het
Itk	T	A	11: 46,336,515 (GRCm38)	Q427L	probably benign	Het
Klhdc4	T	C	8: 121,799,527 (GRCm38)	E291G	probably damaging	Het
Map4	T	A	9: 110,052,819 (GRCm38)	S250R	possibly damaging	Het
Mnt	T	A	11: 74,836,459 (GRCm38)	V57E	possibly damaging	Het
Mrc2	G	A	11: 105,348,431 (GRCm38)		probably null	Het
Musk	A	T	4: 58,301,625 (GRCm38)	I128F	probably damaging	Het
Myh4	G	A	11: 67,250,310 (GRCm38)	A733T	possibly damaging	Het
Myo5a	T	A	9: 75,136,388 (GRCm38)	F220I	probably damaging	Het
Npas3	A	G	12: 54,044,578 (GRCm38)	H305R	probably damaging	Het
Nrcam	A	T	12: 44,591,056 (GRCm38)	Y1132F	probably damaging	Het
Nt5c3b	A	C	11: 100,432,918 (GRCm38)	D189E	probably benign	Het
Nuf2	A	G	1: 169,506,114 (GRCm38)	L331P	probably damaging	Het
Nup214	C	T	2: 31,997,965 (GRCm38)	T646I	probably benign	Het
Olfr112	T	C	17: 37,564,185 (GRCm38)	N42S	probably damaging	Het
Pcdh18	T	C	3: 49,744,725 (GRCm38)	E1096G	probably damaging	Het
Pgm1	T	A	5: 64,107,727 (GRCm38)	F364I	probably benign	Het
Pikfyve	A	G	1: 65,234,262 (GRCm38)	N612S	probably damaging	Het
Pla2g4e	T	G	2: 120,186,382 (GRCm38)	H226P	possibly damaging	Het
Ppp3cb	T	C	14: 20,520,646 (GRCm38)	N339S	possibly damaging	Het
Prkd2	G	T	7: 16,843,648 (GRCm38)		probably benign	Het
Rnf133	T	C	6: 23,649,042 (GRCm38)	E296G	possibly damaging	Het
Sav1	A	T	12: 69,984,321 (GRCm38)	D142E	probably benign	Het
Scn3b	C	A	9: 40,288,423 (GRCm38)	P212T	probably damaging	Het
Sel1l	A	T	12: 91,833,053 (GRCm38)		probably null	Het
Sept9	A	G	11: 117,360,484 (GRCm38)	K543E	probably damaging	Het
Serpinb12	T	A	1: 106,949,153 (GRCm38)	D66E	probably benign	Het
Slc35e2	T	C	4: 155,617,649 (GRCm38)	F290S	probably benign	Het
Snrpa	A	G	7: 27,195,533 (GRCm38)	M1T	probably null	Het
Soga1	T	C	2: 157,039,924 (GRCm38)	K736R	probably benign	Het
Sptlc3	G	A	2: 139,636,680 (GRCm38)	V520I	probably benign	Het
Srpk3	A	G	X: 73,774,941 (GRCm38)	H79R	possibly damaging	Het
Stox2	A	G	8: 47,192,935 (GRCm38)	S497P	probably damaging	Het
Stradb	G	A	1: 58,993,572 (GRCm38)	S361N	probably damaging	Het
Sult3a2	T	C	10: 33,782,087 (GRCm38)	K10R	probably benign	Het
Susd4	A	G	1: 182,858,460 (GRCm38)	N192D	probably damaging	Het
Tcp10a	T	A	17: 7,325,975 (GRCm38)	D32E	probably benign	Het
Tdpoz4	T	A	3: 93,797,032 (GRCm38)	V212D	probably damaging	Het
Tdrd5	T	A	1: 156,284,374 (GRCm38)	T479S	probably benign	Het
Tex10	T	C	4: 48,452,946 (GRCm38)	D671G	probably benign	Het
Traj37	T	C	14: 54,181,539 (GRCm38)		probably benign	Het
Ttc7b	G	A	12: 100,500,117 (GRCm38)	R79C	probably damaging	Het
Vmn2r115	T	A	17: 23,346,399 (GRCm38)	L420Q	probably benign	Het
Wdr24	T	A	17: 25,828,207 (GRCm38)		probably null	Het
Wdr81	T	A	11: 75,445,658 (GRCm38)	Q516L	probably damaging	Het
Zfp516	A	G	18: 82,956,039 (GRCm38)	T121A	probably benign	Het
Zmat1	A	T	X: 134,972,945 (GRCm38)	S566T	probably damaging	Homo

Other mutations in Mslnl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01621:Mslnl	APN	17	25,743,667 (GRCm38)	unclassified	probably benign
IGL01629:Mslnl	APN	17	25,744,775 (GRCm38)	missense	possibly damaging	0.95
IGL02084:Mslnl	APN	17	25,746,151 (GRCm38)	missense	probably benign	0.07
IGL02408:Mslnl	APN	17	25,747,998 (GRCm38)	missense	possibly damaging	0.80
IGL02726:Mslnl	APN	17	25,744,103 (GRCm38)	critical splice donor site	probably null
IGL03387:Mslnl	APN	17	25,744,077 (GRCm38)	missense	probably benign	0.06
R0561:Mslnl	UTSW	17	25,743,203 (GRCm38)	nonsense	probably null
R0881:Mslnl	UTSW	17	25,742,965 (GRCm38)	missense	possibly damaging	0.82
R1295:Mslnl	UTSW	17	25,743,240 (GRCm38)	missense	probably damaging	1.00
R1296:Mslnl	UTSW	17	25,743,240 (GRCm38)	missense	probably damaging	1.00
R1582:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1629:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1630:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1631:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1632:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1794:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1850:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1866:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1876:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1914:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2166:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2241:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2243:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2247:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2282:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2284:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2852:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2877:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2878:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2919:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2920:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3026:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3405:Mslnl	UTSW	17	25,746,181 (GRCm38)	missense	probably damaging	1.00
R3406:Mslnl	UTSW	17	25,746,181 (GRCm38)	missense	probably damaging	1.00
R3411:Mslnl	UTSW	17	25,744,517 (GRCm38)	missense	probably benign	0.05
R3434:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3546:Mslnl	UTSW	17	25,744,969 (GRCm38)	missense	probably damaging	0.98
R3612:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3729:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3730:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3802:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3804:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3894:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3895:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4454:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4455:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4456:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4457:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4561:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4562:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4564:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4600:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4610:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4704:Mslnl	UTSW	17	25,738,978 (GRCm38)	missense	possibly damaging	0.73
R5155:Mslnl	UTSW	17	25,738,968 (GRCm38)	nonsense	probably null
R5257:Mslnl	UTSW	17	25,746,165 (GRCm38)	missense	probably benign	0.00
R5456:Mslnl	UTSW	17	25,743,159 (GRCm38)	missense	probably damaging	0.98
R5645:Mslnl	UTSW	17	25,737,842 (GRCm38)	missense	possibly damaging	0.95
R6007:Mslnl	UTSW	17	25,746,775 (GRCm38)	missense	probably benign	0.00
R6083:Mslnl	UTSW	17	25,737,902 (GRCm38)	missense	possibly damaging	0.83
R6142:Mslnl	UTSW	17	25,744,557 (GRCm38)	missense	probably damaging	1.00
R6761:Mslnl	UTSW	17	25,746,073 (GRCm38)	missense	probably damaging	1.00
R7058:Mslnl	UTSW	17	25,743,212 (GRCm38)	missense	probably benign	0.03
R7156:Mslnl	UTSW	17	25,743,210 (GRCm38)	missense	probably benign	0.20
R7467:Mslnl	UTSW	17	25,736,921 (GRCm38)	start codon destroyed	probably benign	0.33
R7687:Mslnl	UTSW	17	25,743,183 (GRCm38)	missense	probably damaging	0.97
R7807:Mslnl	UTSW	17	25,746,777 (GRCm38)	missense	probably benign	0.03
R8682:Mslnl	UTSW	17	25,746,988 (GRCm38)	missense	probably benign
R8735:Mslnl	UTSW	17	25,745,088 (GRCm38)	missense	probably benign	0.09
R8742:Mslnl	UTSW	17	25,745,073 (GRCm38)	missense	probably damaging	1.00
R9208:Mslnl	UTSW	17	25,742,720 (GRCm38)	missense	possibly damaging	0.94
R9264:Mslnl	UTSW	17	25,742,532 (GRCm38)	intron	probably benign
RF007:Mslnl	UTSW	17	25,743,228 (GRCm38)	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- CCATCAGAAGGGAGGTTTGAAC -3'
(R):5'- AGAAGCCACAGGTCTGAAGC -3'

Sequencing Primer
(F):5'- TTTGAACCCAGGACTGAGGTGAC -3'
(R):5'- CCACAGGTCTGAAGCACTGAG -3'

Posted On

2015-09-25