Mutagenetix > Incidental Mutation

Incidental Mutation 'R0255:Rusc2'

34645

Institutional Source

Beutler Lab

Gene Symbol

Rusc2

Ensembl Gene

ENSMUSG00000035969

Gene Name

RUN and SH3 domain containing 2

Synonyms

MMRRC Submission

038486-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.158) question?

Stock #

R0255 (G1)

Quality Score

212

Status

Validated

Chromosome

Chromosomal Location

43381979-43427088 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 43423954 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 1036 (V1036A)

Ref Sequence

ENSEMBL: ENSMUSP00000118528 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000035645] [ENSMUST00000052829] [ENSMUST00000098106] [ENSMUST00000107928] [ENSMUST00000107929] [ENSMUST00000131668] [ENSMUST00000149221] [ENSMUST00000149676] [ENSMUST00000173682] [ENSMUST00000171134]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000035645
AA Change: V1036A

PolyPhen 2 Score 0.540 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000038379
Gene: ENSMUSG00000035969
AA Change: V1036A

Domain	Start	End	E-Value	Type
low complexity region	39	47	N/A	INTRINSIC
low complexity region	212	230	N/A	INTRINSIC
low complexity region	253	265	N/A	INTRINSIC
low complexity region	411	427	N/A	INTRINSIC
low complexity region	435	448	N/A	INTRINSIC
low complexity region	530	544	N/A	INTRINSIC
low complexity region	600	617	N/A	INTRINSIC
low complexity region	795	809	N/A	INTRINSIC
RUN	1109	1177	3.66e-21	SMART
low complexity region	1235	1260	N/A	INTRINSIC
low complexity region	1289	1324	N/A	INTRINSIC
low complexity region	1330	1341	N/A	INTRINSIC
SH3	1457	1512	7.4e-11	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000052829

SMART Domains

Protein: ENSMUSP00000058980
Gene: ENSMUSG00000042788

Domain	Start	End	E-Value	Type
Pfam:DUF2475	15	47	2.9e-12	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000098106
AA Change: V1036A

PolyPhen 2 Score 0.540 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000095710
Gene: ENSMUSG00000035969
AA Change: V1036A

Domain	Start	End	E-Value	Type
low complexity region	39	47	N/A	INTRINSIC
low complexity region	212	230	N/A	INTRINSIC
low complexity region	253	265	N/A	INTRINSIC
low complexity region	411	427	N/A	INTRINSIC
low complexity region	435	448	N/A	INTRINSIC
low complexity region	530	544	N/A	INTRINSIC
low complexity region	600	617	N/A	INTRINSIC
low complexity region	795	809	N/A	INTRINSIC
RUN	1109	1177	3.66e-21	SMART
low complexity region	1235	1260	N/A	INTRINSIC
low complexity region	1289	1324	N/A	INTRINSIC
low complexity region	1330	1341	N/A	INTRINSIC
SH3	1457	1512	7.4e-11	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000107928

SMART Domains

Protein: ENSMUSP00000103561
Gene: ENSMUSG00000042788

Domain	Start	End	E-Value	Type
Pfam:DUF2475	15	76	1.3e-20	PFAM
Pfam:DUF2475	212	251	6.9e-10	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000107929

SMART Domains

Protein: ENSMUSP00000103562
Gene: ENSMUSG00000042788

Domain	Start	End	E-Value	Type
Pfam:DUF2475	15	76	1.5e-20	PFAM
Pfam:DUF2475	232	271	7.7e-10	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000123447

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000125393

Predicted Effect

probably damaging
Transcript: ENSMUST00000131668
AA Change: V1036A

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000118528
Gene: ENSMUSG00000035969
AA Change: V1036A

Domain	Start	End	E-Value	Type
low complexity region	39	47	N/A	INTRINSIC
low complexity region	212	230	N/A	INTRINSIC
low complexity region	253	265	N/A	INTRINSIC
low complexity region	411	427	N/A	INTRINSIC
low complexity region	435	448	N/A	INTRINSIC
low complexity region	530	544	N/A	INTRINSIC
low complexity region	600	617	N/A	INTRINSIC
low complexity region	795	809	N/A	INTRINSIC
RUN	1109	1177	3.66e-21	SMART
low complexity region	1235	1260	N/A	INTRINSIC
low complexity region	1289	1324	N/A	INTRINSIC
low complexity region	1330	1341	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000146710

Predicted Effect

probably benign
Transcript: ENSMUST00000149221

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000149556

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000150066

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000154754

Predicted Effect

probably benign
Transcript: ENSMUST00000149676

Predicted Effect

probably benign
Transcript: ENSMUST00000173682

SMART Domains

Protein: ENSMUSP00000133715
Gene: ENSMUSG00000035969

Domain	Start	End	E-Value	Type
low complexity region	39	47	N/A	INTRINSIC
low complexity region	212	230	N/A	INTRINSIC
low complexity region	253	265	N/A	INTRINSIC
low complexity region	411	427	N/A	INTRINSIC
low complexity region	435	448	N/A	INTRINSIC
low complexity region	530	544	N/A	INTRINSIC
low complexity region	685	703	N/A	INTRINSIC
low complexity region	733	740	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000155080

Predicted Effect

probably benign
Transcript: ENSMUST00000171134

SMART Domains

Protein: ENSMUSP00000127145
Gene: ENSMUSG00000042788

Domain	Start	End	E-Value	Type
Pfam:DUF2475	15	76	7.2e-21	PFAM

Meta Mutation Damage Score

0.1143

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 95.6%

Validation Efficiency

93% (102/110)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a RUN and SH3 domain containing protein that interacts with Rab1b and Rab1-binding protein GM130. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jun 2012]

Allele List at MGI

Other mutations in this stock

Total: 108 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700113H08Rik	T	C	10: 87,226,045 (GRCm38)	F119S	probably damaging	Het
1810011H11Rik	A	G	14: 32,808,363 (GRCm38)	K83E	possibly damaging	Het
Abca13	C	A	11: 9,581,545 (GRCm38)	Q4591K	probably damaging	Het
Anapc1	A	T	2: 128,634,711 (GRCm38)	M1329K	probably damaging	Het
Aoah	A	T	13: 20,979,540 (GRCm38)	K338*	probably null	Het
Ascc3	A	T	10: 50,645,058 (GRCm38)	T416S	probably benign	Het
Baz2a	A	G	10: 128,114,639 (GRCm38)	T484A	possibly damaging	Het
Btnl6	T	C	17: 34,508,503 (GRCm38)	N351S	probably benign	Het
Cacna1s	T	G	1: 136,118,806 (GRCm38)	I1772S	possibly damaging	Het
Ccdc8	A	G	7: 16,995,657 (GRCm38)	D357G	unknown	Het
Ccna1	T	C	3: 55,050,628 (GRCm38)	E152G	probably damaging	Het
Cct4	A	G	11: 22,999,073 (GRCm38)	D273G	probably damaging	Het
Cd9	A	G	6: 125,463,740 (GRCm38)	V96A	probably damaging	Het
Cdc5l	G	A	17: 45,415,684 (GRCm38)	R321W	probably damaging	Het
Cdh7	T	C	1: 109,994,306 (GRCm38)	S43P	probably benign	Het
Cep95	G	A	11: 106,811,271 (GRCm38)	V365M	probably benign	Het
Ces1c	T	C	8: 93,127,524 (GRCm38)	T128A	probably benign	Het
Chil5	A	G	3: 106,019,267 (GRCm38)	V82A	probably damaging	Het
Clmn	T	C	12: 104,781,764 (GRCm38)	D508G	probably benign	Het
Cog8	A	T	8: 107,049,145 (GRCm38)		probably benign	Het
Cst3	A	T	2: 148,875,169 (GRCm38)	V70E	probably damaging	Het
Ctcf	A	G	8: 105,664,039 (GRCm38)	T93A	possibly damaging	Het
Ctsk	C	A	3: 95,508,877 (GRCm38)	N315K	probably benign	Het
Cyp2j12	G	T	4: 96,141,025 (GRCm38)	D6E	probably benign	Het
Dhx34	A	G	7: 16,205,992 (GRCm38)	V655A	probably benign	Het
Dock10	T	C	1: 80,605,876 (GRCm38)	Y203C	probably damaging	Het
Dok7	A	T	5: 35,064,334 (GRCm38)	D26V	probably damaging	Het
Epha8	G	A	4: 136,940,286 (GRCm38)	H295Y	probably damaging	Het
Esf1	A	G	2: 140,148,923 (GRCm38)		probably benign	Het
Fam83c	A	G	2: 155,829,752 (GRCm38)	S588P	probably benign	Het
Fat3	G	A	9: 15,969,706 (GRCm38)		probably benign	Het
Fhdc1	T	C	3: 84,453,510 (GRCm38)		probably benign	Het
Frmd4b	A	G	6: 97,308,086 (GRCm38)	V338A	probably damaging	Het
Fsd1l	A	G	4: 53,694,727 (GRCm38)	T394A	probably damaging	Het
Gbf1	A	G	19: 46,254,110 (GRCm38)		probably benign	Het
Glg1	G	T	8: 111,159,858 (GRCm38)	Q1101K	possibly damaging	Het
Glt8d2	T	A	10: 82,651,527 (GRCm38)		probably null	Het
Gm19345	T	C	7: 19,854,930 (GRCm38)		probably benign	Het
Gpr179	A	T	11: 97,336,066 (GRCm38)	D1754E	probably benign	Het
Hydin	A	G	8: 110,565,018 (GRCm38)	T3381A	probably benign	Het
Igkv12-41	G	A	6: 69,858,838 (GRCm38)	T16I	possibly damaging	Het
Insl5	A	G	4: 103,018,116 (GRCm38)	*146Q	probably null	Het
Iqce	A	T	5: 140,666,202 (GRCm38)	I655N	possibly damaging	Het
Irf2bp1	G	A	7: 19,005,002 (GRCm38)	R189H	possibly damaging	Het
Itsn1	C	T	16: 91,806,090 (GRCm38)		probably benign	Het
Kansl3	A	T	1: 36,344,969 (GRCm38)	I724N	probably benign	Het
Kcna4	T	C	2: 107,296,562 (GRCm38)	I547T	probably damaging	Het
Klk1b4	A	T	7: 44,210,734 (GRCm38)	I91F	probably benign	Het
Lmbr1	A	G	5: 29,252,755 (GRCm38)	S282P	probably damaging	Het
Lrrc17	G	A	5: 21,560,969 (GRCm38)	A150T	probably benign	Het
Lrrc2	A	G	9: 110,980,898 (GRCm38)	E334G	possibly damaging	Het
Lrrc7	G	A	3: 158,160,838 (GRCm38)	Q1077*	probably null	Het
Mapk8	A	T	14: 33,387,307 (GRCm38)		probably benign	Het
Mast1	T	A	8: 84,912,021 (GRCm38)	T1560S	probably benign	Het
Mdh1b	C	A	1: 63,719,618 (GRCm38)	A272S	probably damaging	Het
Myo15b	T	C	11: 115,886,283 (GRCm38)	Y912H	probably damaging	Het
Nf1	T	G	11: 79,408,699 (GRCm38)		probably null	Het
Nme7	T	A	1: 164,345,375 (GRCm38)	D218E	probably damaging	Het
Nsun7	T	A	5: 66,289,408 (GRCm38)		probably benign	Het
Nxpe2	A	G	9: 48,340,570 (GRCm38)		probably null	Het
Olfr135	T	G	17: 38,208,395 (GRCm38)	I50R	probably benign	Het
Olfr394	A	G	11: 73,887,829 (GRCm38)	V181A	probably benign	Het
Olfr473	T	A	7: 107,934,168 (GRCm38)	V216D	probably damaging	Het
Olfr698	G	A	7: 106,752,989 (GRCm38)	T133I	probably benign	Het
P2ry1	T	C	3: 61,003,530 (GRCm38)	V30A	probably benign	Het
Pgm1	T	A	5: 64,112,043 (GRCm38)	I491N	possibly damaging	Het
Pkd1l3	A	T	8: 109,638,754 (GRCm38)	D1169V	probably damaging	Het
Poldip2	T	A	11: 78,512,363 (GRCm38)	S18T	probably benign	Het
Ppp1r32	C	T	19: 10,475,054 (GRCm38)	R364Q	probably damaging	Het
Pqlc1	C	T	18: 80,263,518 (GRCm38)	A101V	probably benign	Het
Prg4	C	T	1: 150,455,807 (GRCm38)		probably benign	Het
Prkab2	T	A	3: 97,667,412 (GRCm38)	Y241*	probably null	Het
Prmt7	A	G	8: 106,227,207 (GRCm38)		probably benign	Het
Proser3	T	A	7: 30,546,417 (GRCm38)	R80W	probably damaging	Het
Prr12	A	G	7: 45,049,991 (GRCm38)		probably benign	Het
Psmd1	A	T	1: 86,078,582 (GRCm38)	L223F	probably damaging	Het
Rab13	A	G	3: 90,223,781 (GRCm38)		probably benign	Het
Rgl1	C	T	1: 152,552,596 (GRCm38)	C294Y	probably damaging	Het
Rpl21-ps4	C	A	14: 11,227,556 (GRCm38)		noncoding transcript	Het
Sae1	T	A	7: 16,370,322 (GRCm38)	K121*	probably null	Het
Sap130	C	T	18: 31,680,506 (GRCm38)	P539S	probably damaging	Het
Scube2	A	G	7: 109,824,872 (GRCm38)	L475P	probably damaging	Het
Sec16a	T	C	2: 26,431,186 (GRCm38)	D1298G	probably damaging	Het
Serpinb9b	T	C	13: 33,038,020 (GRCm38)	F206L	probably benign	Het
Sh3bp1	T	A	15: 78,904,334 (GRCm38)	Y202*	probably null	Het
Shprh	T	A	10: 11,186,391 (GRCm38)	C1177S	possibly damaging	Het
Slc27a6	T	C	18: 58,609,865 (GRCm38)	Y542H	possibly damaging	Het
Slc41a1	T	C	1: 131,843,912 (GRCm38)		probably benign	Het
Slc46a1	T	C	11: 78,470,799 (GRCm38)	F424L	probably damaging	Het
Slc6a12	G	A	6: 121,356,918 (GRCm38)	V238I	probably damaging	Het
Slc6a20a	A	G	9: 123,664,621 (GRCm38)	V65A	probably damaging	Het
Slc9c1	T	A	16: 45,554,300 (GRCm38)	S343T	probably benign	Het
Spcs3	C	A	8: 54,528,380 (GRCm38)	R60I	probably benign	Het
Ssfa2	T	A	2: 79,660,466 (GRCm38)	L976Q	probably damaging	Het
Tbc1d16	C	A	11: 119,147,575 (GRCm38)	R764L	possibly damaging	Het
Tcaf2	A	T	6: 42,642,904 (GRCm38)	V63E	possibly damaging	Het
Tmc1	G	T	19: 20,789,587 (GRCm38)	A750E	possibly damaging	Het
Tmem81	C	G	1: 132,507,829 (GRCm38)	I124M	probably damaging	Het
Tmx3	T	C	18: 90,540,006 (GRCm38)	I394T	probably damaging	Het
Trank1	G	A	9: 111,366,024 (GRCm38)	E1039K	possibly damaging	Het
Trpc4ap	A	G	2: 155,657,946 (GRCm38)		probably benign	Het
Tsen54	T	C	11: 115,815,408 (GRCm38)	C123R	probably damaging	Het
Ubtd1	A	G	19: 42,031,934 (GRCm38)	D39G	possibly damaging	Het
Vmn1r181	A	T	7: 23,984,334 (GRCm38)	M75L	probably benign	Het
Zc3h7a	T	C	16: 11,140,737 (GRCm38)	T847A	probably damaging	Het
Zc3hav1	A	G	6: 38,336,550 (GRCm38)	C187R	probably damaging	Het
Zfp692	G	A	11: 58,310,403 (GRCm38)		probably benign	Het
Zmiz1	G	A	14: 25,654,495 (GRCm38)		probably benign	Het

Other mutations in Rusc2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01358:Rusc2	APN	4	43,426,116 (GRCm38)	missense	probably damaging	0.97
IGL01474:Rusc2	APN	4	43,416,434 (GRCm38)	missense	probably damaging	0.98
IGL01541:Rusc2	APN	4	43,415,840 (GRCm38)	missense	probably benign	0.08
IGL01628:Rusc2	APN	4	43,425,729 (GRCm38)	missense	probably damaging	1.00
IGL01969:Rusc2	APN	4	43,415,738 (GRCm38)	missense	probably benign	0.02
IGL02030:Rusc2	APN	4	43,416,095 (GRCm38)	missense	possibly damaging	0.86
IGL02079:Rusc2	APN	4	43,425,668 (GRCm38)	missense	probably benign
IGL02115:Rusc2	APN	4	43,426,136 (GRCm38)	splice site	probably benign
IGL02122:Rusc2	APN	4	43,421,685 (GRCm38)	missense	possibly damaging	0.67
IGL02350:Rusc2	APN	4	43,425,351 (GRCm38)	missense	possibly damaging	0.86
IGL02357:Rusc2	APN	4	43,425,351 (GRCm38)	missense	possibly damaging	0.86
IGL02437:Rusc2	APN	4	43,415,545 (GRCm38)	missense	probably damaging	1.00
IGL02930:Rusc2	APN	4	43,416,376 (GRCm38)	missense	probably damaging	0.99
IGL03154:Rusc2	APN	4	43,425,806 (GRCm38)	missense	probably benign	0.00
P0026:Rusc2	UTSW	4	43,415,840 (GRCm38)	missense	possibly damaging	0.93
R0036:Rusc2	UTSW	4	43,424,009 (GRCm38)	missense	probably damaging	1.00
R0068:Rusc2	UTSW	4	43,424,100 (GRCm38)	splice site	probably benign
R0068:Rusc2	UTSW	4	43,424,100 (GRCm38)	splice site	probably benign
R0114:Rusc2	UTSW	4	43,422,055 (GRCm38)	missense	probably damaging	1.00
R0471:Rusc2	UTSW	4	43,425,486 (GRCm38)	missense	probably damaging	0.99
R1381:Rusc2	UTSW	4	43,416,137 (GRCm38)	missense	probably damaging	1.00
R1413:Rusc2	UTSW	4	43,416,568 (GRCm38)	missense	probably benign	0.00
R1416:Rusc2	UTSW	4	43,421,617 (GRCm38)	missense	possibly damaging	0.86
R1731:Rusc2	UTSW	4	43,426,046 (GRCm38)	missense	probably benign
R1864:Rusc2	UTSW	4	43,421,719 (GRCm38)	missense	possibly damaging	0.49
R1897:Rusc2	UTSW	4	43,421,749 (GRCm38)	missense	probably damaging	1.00
R2010:Rusc2	UTSW	4	43,415,212 (GRCm38)	missense	probably benign	0.06
R2212:Rusc2	UTSW	4	43,415,935 (GRCm38)	missense	probably damaging	1.00
R2275:Rusc2	UTSW	4	43,416,260 (GRCm38)	missense	probably damaging	1.00
R2885:Rusc2	UTSW	4	43,415,456 (GRCm38)	missense	probably benign	0.28
R2886:Rusc2	UTSW	4	43,415,456 (GRCm38)	missense	probably benign	0.28
R3412:Rusc2	UTSW	4	43,415,935 (GRCm38)	missense	probably damaging	1.00
R3413:Rusc2	UTSW	4	43,415,935 (GRCm38)	missense	probably damaging	1.00
R3414:Rusc2	UTSW	4	43,415,935 (GRCm38)	missense	probably damaging	1.00
R3852:Rusc2	UTSW	4	43,416,424 (GRCm38)	missense	probably benign	0.45
R4135:Rusc2	UTSW	4	43,425,563 (GRCm38)	missense	possibly damaging	0.49
R4272:Rusc2	UTSW	4	43,415,533 (GRCm38)	missense	probably damaging	1.00
R4574:Rusc2	UTSW	4	43,416,080 (GRCm38)	missense	probably damaging	0.99
R4888:Rusc2	UTSW	4	43,423,942 (GRCm38)	missense	probably damaging	1.00
R5010:Rusc2	UTSW	4	43,415,926 (GRCm38)	missense	probably damaging	1.00
R5071:Rusc2	UTSW	4	43,415,240 (GRCm38)	missense	probably benign	0.05
R5131:Rusc2	UTSW	4	43,414,948 (GRCm38)	missense	probably benign	0.03
R5177:Rusc2	UTSW	4	43,421,805 (GRCm38)	splice site	probably null
R5540:Rusc2	UTSW	4	43,423,975 (GRCm38)	missense	probably damaging	1.00
R5561:Rusc2	UTSW	4	43,415,932 (GRCm38)	nonsense	probably null
R5628:Rusc2	UTSW	4	43,425,348 (GRCm38)	missense	probably damaging	1.00
R5645:Rusc2	UTSW	4	43,425,758 (GRCm38)	missense	probably benign	0.06
R6129:Rusc2	UTSW	4	43,424,271 (GRCm38)	missense	probably damaging	1.00
R6362:Rusc2	UTSW	4	43,416,416 (GRCm38)	missense	probably benign	0.30
R6633:Rusc2	UTSW	4	43,414,852 (GRCm38)	missense	probably damaging	0.99
R6980:Rusc2	UTSW	4	43,422,846 (GRCm38)	missense	probably benign	0.35
R7491:Rusc2	UTSW	4	43,426,528 (GRCm38)	missense	probably damaging	1.00
R7641:Rusc2	UTSW	4	43,425,335 (GRCm38)	missense	possibly damaging	0.84
R7698:Rusc2	UTSW	4	43,414,900 (GRCm38)	nonsense	probably null
R7710:Rusc2	UTSW	4	43,416,119 (GRCm38)	missense	probably benign	0.07
R8052:Rusc2	UTSW	4	43,421,851 (GRCm38)	missense	probably benign
R8061:Rusc2	UTSW	4	43,422,492 (GRCm38)	missense	probably damaging	1.00
R8127:Rusc2	UTSW	4	43,423,747 (GRCm38)	missense	possibly damaging	0.54
R8319:Rusc2	UTSW	4	43,425,378 (GRCm38)	missense	probably damaging	1.00
R8355:Rusc2	UTSW	4	43,422,846 (GRCm38)	missense	probably benign	0.35
R8397:Rusc2	UTSW	4	43,424,206 (GRCm38)	missense	possibly damaging	0.95
R8455:Rusc2	UTSW	4	43,422,846 (GRCm38)	missense	probably benign	0.35
R8553:Rusc2	UTSW	4	43,416,508 (GRCm38)	missense	probably benign	0.05
R8725:Rusc2	UTSW	4	43,401,351 (GRCm38)	intron	probably benign
R8725:Rusc2	UTSW	4	43,415,396 (GRCm38)	missense	probably damaging	0.99
R8727:Rusc2	UTSW	4	43,401,351 (GRCm38)	intron	probably benign
R8834:Rusc2	UTSW	4	43,416,431 (GRCm38)	missense	possibly damaging	0.94
R9295:Rusc2	UTSW	4	43,416,382 (GRCm38)	missense	probably damaging	0.98
R9483:Rusc2	UTSW	4	43,415,897 (GRCm38)	missense	probably damaging	0.97
R9666:Rusc2	UTSW	4	43,416,262 (GRCm38)	missense	probably benign	0.21
R9705:Rusc2	UTSW	4	43,424,936 (GRCm38)	missense	probably benign	0.00
X0025:Rusc2	UTSW	4	43,422,226 (GRCm38)	missense	probably benign	0.00
X0066:Rusc2	UTSW	4	43,422,204 (GRCm38)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- CACTATCAAGAGCTGAGCTATGCCC -3'
(R):5'- TGTTGTAGAGGCCATGCAAGACC -3'

Sequencing Primer
(F):5'- GGTCTGACTAAGCCAGACTAAG -3'
(R):5'- CATGCAAGACCTTGGTGGATG -3'

Posted On

2013-05-09