Mutagenetix > Incidental Mutation

Incidental Mutation 'R4672:Otog'

348529

Institutional Source

Beutler Lab

Gene Symbol

Otog

Ensembl Gene

ENSMUSG00000009487

Gene Name

otogelin

Synonyms

Otgn

MMRRC Submission

041927-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.770) question?

Stock #

R4672 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

45890411-45960858 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 45939210 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Aspartic acid at position 2080 (A2080D)

Ref Sequence

ENSEMBL: ENSMUSP00000130949 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164538]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000164538
AA Change: A2080D

PolyPhen 2 Score 0.980 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000130949
Gene: ENSMUSG00000009487
AA Change: A2080D

Domain	Start	End	E-Value	Type
signal peptide	1	25	N/A	INTRINSIC
low complexity region	72	85	N/A	INTRINSIC
VWD	128	288	7.98e-45	SMART
C8	330	404	1.05e-13	SMART
VWC	463	505	1.24e0	SMART
VWD	490	655	4.94e-50	SMART
C8	693	758	1.23e-5	SMART
Pfam:TIL	767	831	3.4e-13	PFAM
VWC	935	983	1.83e0	SMART
VWD	962	1118	6.05e-45	SMART
C8	1153	1227	1.02e-34	SMART
Pfam:AbfB	1270	1384	7.5e-10	PFAM
low complexity region	1488	1513	N/A	INTRINSIC
low complexity region	1524	1536	N/A	INTRINSIC
low complexity region	1560	1578	N/A	INTRINSIC
low complexity region	1637	1644	N/A	INTRINSIC
low complexity region	1677	1696	N/A	INTRINSIC
low complexity region	1731	1748	N/A	INTRINSIC
VWD	2087	2251	2.37e-29	SMART
C8	2287	2356	4.93e-19	SMART
low complexity region	2443	2449	N/A	INTRINSIC
CT	2828	2911	3.46e-28	SMART

Meta Mutation Damage Score

0.5412

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.1%
20x: 94.8%

Validation Efficiency

98% (113/115)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a component of the acellular membranes of the inner ear. Disruption of the orthologous mouse gene shows that it plays a role in auditory and vestibular functions. It is involved in fibrillar network organization, the anchoring of otoconial membranes and cupulae to the neuroepithelia, and likely in sound stimulation resistance. Mutations in this gene cause autosomal recessive nonsyndromic deafness, type 18B. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2014]
PHENOTYPE: Homozygotes for a number of different spontaneous and targeted mutations exhibit vestibular dysfunction, including circling, head tilt, impaired balance, coordination, and placing response. Mutants have impaired hearing, decreased brain stem auditory evoked potential, and ear abnormalities. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 105 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca8a	G	A	11: 109,962,702 (GRCm39)	L483F	possibly damaging	Het
Abca8b	A	G	11: 109,827,274 (GRCm39)	F1507L	possibly damaging	Het
Adamdec1	C	A	14: 68,815,353 (GRCm39)	E104*	probably null	Het
Adamts16	G	A	13: 70,927,637 (GRCm39)		probably benign	Het
Alppl2	T	C	1: 87,017,187 (GRCm39)		probably benign	Het
Aplnr	A	G	2: 84,967,524 (GRCm39)	Y183C	probably damaging	Het
Atm	G	A	9: 53,433,501 (GRCm39)	R250W	probably damaging	Het
B4galt7	T	C	13: 55,757,132 (GRCm39)	L275P	probably damaging	Het
Bltp1	A	G	3: 36,944,139 (GRCm39)	*330W	probably null	Het
Ccdc178	G	A	18: 22,283,501 (GRCm39)	Q10*	probably null	Het
Ccr9	T	C	9: 123,608,752 (GRCm39)	Y145H	probably damaging	Het
Cd209f	C	T	8: 4,153,685 (GRCm39)	G188D	probably damaging	Het
Cep70	T	A	9: 99,136,365 (GRCm39)	S23T	possibly damaging	Het
Cpped1	C	A	16: 11,623,238 (GRCm39)	E294*	probably null	Het
Crisp1	T	A	17: 40,605,404 (GRCm39)		probably null	Het
Disp1	T	C	1: 182,880,215 (GRCm39)		probably null	Het
Dlg2	A	T	7: 91,935,743 (GRCm39)	M624L	probably damaging	Het
Elf2	A	G	3: 51,163,855 (GRCm39)	V558A	probably damaging	Het
Eno2	T	C	6: 124,743,109 (GRCm39)	D209G	probably damaging	Het
Fam187b	G	A	7: 30,676,968 (GRCm39)	R159H	probably damaging	Het
Fh1	G	A	1: 175,431,617 (GRCm39)	A423V	probably benign	Het
Frg1	C	T	8: 41,853,846 (GRCm39)	D164N	probably benign	Het
Fsbp	T	G	4: 11,579,841 (GRCm39)	N36K	probably benign	Het
Gcn1	A	G	5: 115,744,579 (GRCm39)	T1592A	probably damaging	Het
Gimap3	A	G	6: 48,742,687 (GRCm39)	I81T	probably damaging	Het
Gjb6	C	T	14: 57,362,235 (GRCm39)	V9I	probably benign	Het
Gkap1	T	C	13: 58,411,770 (GRCm39)	S68G	possibly damaging	Het
Gpatch8	A	T	11: 102,369,784 (GRCm39)	S1251R	probably damaging	Het
Gria4	A	G	9: 4,664,981 (GRCm39)	F92L	possibly damaging	Het
H2-Q1	C	A	17: 35,539,906 (GRCm39)	D58E	probably damaging	Het
Hs1bp3	G	T	12: 8,391,983 (GRCm39)	G362*	probably null	Het
Igfn1	T	A	1: 135,893,107 (GRCm39)	H2114L	possibly damaging	Het
Igkv12-98	A	G	6: 68,547,940 (GRCm39)	Q22R	probably benign	Het
Ing3	A	G	6: 21,965,729 (GRCm39)		probably null	Het
Insr	C	T	8: 3,217,501 (GRCm39)		probably null	Het
Kdm3b	T	C	18: 34,941,630 (GRCm39)	S374P	probably benign	Het
Kif14	A	C	1: 136,449,016 (GRCm39)	Q1472P	probably benign	Het
Kif14	G	T	1: 136,449,017 (GRCm39)	Q1472H	probably benign	Het
Knstrn	A	G	2: 118,664,512 (GRCm39)	E202G	probably damaging	Het
Knstrn	G	T	2: 118,664,513 (GRCm39)	E202D	possibly damaging	Het
Krt12	A	G	11: 99,309,509 (GRCm39)		probably benign	Het
Lgi3	A	T	14: 70,771,897 (GRCm39)	I195F	possibly damaging	Het
Lima1	T	C	15: 99,741,590 (GRCm39)	N29D	probably damaging	Het
Liph	T	C	16: 21,802,806 (GRCm39)	I88V	probably benign	Het
Lrrk1	A	G	7: 65,929,120 (GRCm39)	S86P	probably benign	Het
Lsamp	A	G	16: 41,775,697 (GRCm39)	R166G	probably damaging	Het
Mamdc2	T	A	19: 23,328,148 (GRCm39)	N407Y	probably damaging	Het
Mast3	CATA	CA	8: 71,237,441 (GRCm39)		probably null	Het
Megf6	T	A	4: 154,333,909 (GRCm39)	N212K	probably damaging	Het
Met	A	C	6: 17,571,803 (GRCm39)	D1374A	probably benign	Het
Mrc2	A	G	11: 105,233,923 (GRCm39)	T902A	probably benign	Het
Mroh3	T	A	1: 136,118,713 (GRCm39)	T535S	probably benign	Het
Muc1	G	T	3: 89,139,384 (GRCm39)	V595L	probably damaging	Het
Myh2	T	A	11: 67,079,303 (GRCm39)	L957Q	probably damaging	Het
Myorg	T	A	4: 41,499,061 (GRCm39)	M190L	probably benign	Het
Ncl	A	T	1: 86,284,324 (GRCm39)	D257E	probably benign	Het
Nipbl	T	A	15: 8,332,468 (GRCm39)	D2263V	probably damaging	Het
Optc	C	A	1: 133,825,555 (GRCm39)	V324L	possibly damaging	Het
Or4k15	A	G	14: 50,364,714 (GRCm39)	N227D	probably benign	Het
Or4k42	A	T	2: 111,319,902 (GRCm39)	N200K	possibly damaging	Het
Or7g35	A	G	9: 19,496,726 (GRCm39)	K298E	possibly damaging	Het
Osbpl9	A	G	4: 108,921,806 (GRCm39)	I604T	possibly damaging	Het
Parp6	G	C	9: 59,547,393 (GRCm39)	R460P	probably damaging	Het
Phactr4	G	T	4: 132,098,017 (GRCm39)	P417Q	probably damaging	Het
Pigt	T	C	2: 164,339,498 (GRCm39)		probably benign	Het
Plekha5	A	G	6: 140,470,655 (GRCm39)	I99V	probably damaging	Het
Plxna3	T	G	X: 73,382,554 (GRCm39)		probably null	Het
Ppp2r1b	G	A	9: 50,779,019 (GRCm39)	M362I	probably damaging	Het
Pramel34	T	A	5: 93,784,182 (GRCm39)	R230S	probably damaging	Het
Rad51	A	G	2: 118,954,327 (GRCm39)	I136V	probably benign	Het
Rad54b	T	A	4: 11,609,449 (GRCm39)	H633Q	probably benign	Het
Rasal2	A	G	1: 157,071,231 (GRCm39)	F41S	probably benign	Het
Reep3	T	A	10: 66,857,629 (GRCm39)	H154L	probably benign	Het
Rp1l1	T	G	14: 64,268,719 (GRCm39)	V1435G	probably damaging	Het
Rps6ka5	C	A	12: 100,620,546 (GRCm39)	K125N	possibly damaging	Het
Rsad1	A	T	11: 94,434,444 (GRCm39)	M330K	probably damaging	Het
Scand1	A	G	2: 156,153,850 (GRCm39)		probably null	Het
Setd6	A	G	8: 96,444,640 (GRCm39)	H111R	probably null	Het
Slc27a3	T	C	3: 90,294,953 (GRCm39)	N368S	possibly damaging	Het
Slc38a2	T	C	15: 96,596,518 (GRCm39)	T32A	probably benign	Het
Smg7	A	G	1: 152,721,164 (GRCm39)	S683P	probably damaging	Het
Smyd2	A	T	1: 189,642,101 (GRCm39)	L62M	probably damaging	Het
Sox5	T	C	6: 143,779,075 (GRCm39)	Y687C	probably damaging	Het
Spaca6	T	A	17: 18,057,005 (GRCm39)	C53*	probably null	Het
Spata31e4	T	C	13: 50,857,208 (GRCm39)	Y949H	probably benign	Het
Spire2	T	C	8: 124,084,850 (GRCm39)	V230A	probably benign	Het
Sptbn2	G	T	19: 4,782,524 (GRCm39)	V487L	probably benign	Het
Stk3	T	A	15: 35,099,603 (GRCm39)	I110L	probably benign	Het
Stox2	T	A	8: 47,645,141 (GRCm39)	Y773F	probably damaging	Het
Tbrg1	C	A	9: 37,562,632 (GRCm39)	A259S	probably damaging	Het
Tnfsf18	C	A	1: 161,331,307 (GRCm39)	D152E	probably benign	Het
Tpr	G	A	1: 150,299,318 (GRCm39)	A1173T	probably benign	Het
Trrap	T	C	5: 144,722,290 (GRCm39)	L271P	probably damaging	Het
Ttn	G	T	2: 76,657,419 (GRCm39)		probably benign	Het
U2surp	A	G	9: 95,375,198 (GRCm39)	S192P	possibly damaging	Het
Ubr4	C	T	4: 139,138,027 (GRCm39)	S1128L	probably damaging	Het
Ucma	G	A	2: 4,981,465 (GRCm39)		probably null	Het
Urb1	A	T	16: 90,569,522 (GRCm39)	D1401E	probably benign	Het
Usp54	A	T	14: 20,631,597 (GRCm39)		probably benign	Het
Vmn1r31	A	G	6: 58,449,056 (GRCm39)	Y270H	probably damaging	Het
Vmn1r90	T	A	7: 14,295,493 (GRCm39)	T202S	probably benign	Het
Vmn2r88	A	G	14: 51,655,612 (GRCm39)	Y616C	probably damaging	Het
Vmn2r95	T	A	17: 18,672,413 (GRCm39)	W717R	probably damaging	Het
Zcchc4	C	A	5: 52,953,947 (GRCm39)	T209K	probably benign	Het
Zfp955b	T	A	17: 33,524,233 (GRCm39)		probably benign	Het

Other mutations in Otog

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00516:Otog	APN	7	45,900,706 (GRCm39)	missense	probably damaging	1.00
IGL00725:Otog	APN	7	45,923,516 (GRCm39)	missense	probably damaging	1.00
IGL00757:Otog	APN	7	45,939,552 (GRCm39)	missense	probably damaging	1.00
IGL00822:Otog	APN	7	45,945,304 (GRCm39)	missense	probably benign	0.24
IGL01354:Otog	APN	7	45,939,150 (GRCm39)	missense	probably damaging	1.00
IGL01567:Otog	APN	7	45,926,039 (GRCm39)	splice site	probably benign
IGL02034:Otog	APN	7	45,945,417 (GRCm39)	nonsense	probably null
IGL02090:Otog	APN	7	45,949,571 (GRCm39)	missense	probably damaging	1.00
IGL02132:Otog	APN	7	45,954,903 (GRCm39)	missense	probably damaging	0.99
IGL02148:Otog	APN	7	45,950,011 (GRCm39)	missense	probably damaging	1.00
IGL02173:Otog	APN	7	45,926,165 (GRCm39)	splice site	probably benign
IGL02199:Otog	APN	7	45,926,775 (GRCm39)	missense	possibly damaging	0.90
IGL02216:Otog	APN	7	45,950,892 (GRCm39)	missense	probably damaging	1.00
IGL02322:Otog	APN	7	45,950,881 (GRCm39)	missense	probably benign	0.01
IGL02330:Otog	APN	7	45,937,493 (GRCm39)	missense	possibly damaging	0.84
IGL02529:Otog	APN	7	45,909,381 (GRCm39)	missense	probably damaging	0.99
IGL02898:Otog	APN	7	45,959,562 (GRCm39)	missense	probably damaging	1.00
IGL02970:Otog	APN	7	45,945,291 (GRCm39)	missense	probably benign	0.11
IGL03085:Otog	APN	7	45,955,346 (GRCm39)	critical splice donor site	probably null
IGL03108:Otog	APN	7	45,900,762 (GRCm39)	missense	probably damaging	1.00
IGL03275:Otog	APN	7	45,955,654 (GRCm39)	missense	probably damaging	1.00
R0282_Otog_616	UTSW	7	45,926,917 (GRCm39)	missense	possibly damaging	0.93
R0636_otog_678	UTSW	7	45,913,652 (GRCm39)	critical splice donor site	probably null
R1029_otog_141	UTSW	7	45,924,019 (GRCm39)	missense	probably damaging	1.00
BB010:Otog	UTSW	7	45,959,571 (GRCm39)	missense	probably damaging	1.00
BB020:Otog	UTSW	7	45,959,571 (GRCm39)	missense	probably damaging	1.00
I1329:Otog	UTSW	7	45,895,927 (GRCm39)	missense	probably benign	0.02
IGL02984:Otog	UTSW	7	45,954,932 (GRCm39)	missense	probably damaging	0.98
PIT4472001:Otog	UTSW	7	45,945,273 (GRCm39)	missense	probably damaging	1.00
R0032:Otog	UTSW	7	45,953,655 (GRCm39)	missense	probably damaging	0.97
R0032:Otog	UTSW	7	45,937,637 (GRCm39)	nonsense	probably null
R0105:Otog	UTSW	7	45,937,790 (GRCm39)	missense	possibly damaging	0.79
R0164:Otog	UTSW	7	45,953,655 (GRCm39)	missense	probably damaging	0.97
R0164:Otog	UTSW	7	45,953,655 (GRCm39)	missense	probably damaging	0.97
R0165:Otog	UTSW	7	45,953,655 (GRCm39)	missense	probably damaging	0.97
R0166:Otog	UTSW	7	45,953,655 (GRCm39)	missense	probably damaging	0.97
R0167:Otog	UTSW	7	45,953,655 (GRCm39)	missense	probably damaging	0.97
R0240:Otog	UTSW	7	45,913,456 (GRCm39)	splice site	probably null
R0240:Otog	UTSW	7	45,913,456 (GRCm39)	splice site	probably null
R0242:Otog	UTSW	7	45,916,805 (GRCm39)	missense	probably damaging	0.98
R0242:Otog	UTSW	7	45,916,805 (GRCm39)	missense	probably damaging	0.98
R0282:Otog	UTSW	7	45,926,917 (GRCm39)	missense	possibly damaging	0.93
R0392:Otog	UTSW	7	45,899,499 (GRCm39)	missense	probably benign	0.00
R0436:Otog	UTSW	7	45,915,360 (GRCm39)	splice site	probably benign
R0441:Otog	UTSW	7	45,955,301 (GRCm39)	missense	probably damaging	1.00
R0499:Otog	UTSW	7	45,923,256 (GRCm39)	missense	probably damaging	1.00
R0530:Otog	UTSW	7	45,947,668 (GRCm39)	missense	probably damaging	0.98
R0541:Otog	UTSW	7	45,918,673 (GRCm39)	splice site	probably benign
R0600:Otog	UTSW	7	45,900,819 (GRCm39)	splice site	probably benign
R0626:Otog	UTSW	7	45,920,797 (GRCm39)	missense	possibly damaging	0.95
R0636:Otog	UTSW	7	45,913,652 (GRCm39)	critical splice donor site	probably null
R0764:Otog	UTSW	7	45,949,918 (GRCm39)	missense	probably benign	0.00
R0833:Otog	UTSW	7	45,918,786 (GRCm39)	missense	possibly damaging	0.94
R0836:Otog	UTSW	7	45,918,786 (GRCm39)	missense	possibly damaging	0.94
R0844:Otog	UTSW	7	45,937,252 (GRCm39)	missense	possibly damaging	0.53
R1029:Otog	UTSW	7	45,924,019 (GRCm39)	missense	probably damaging	1.00
R1116:Otog	UTSW	7	45,950,025 (GRCm39)	splice site	probably benign
R1134:Otog	UTSW	7	45,947,938 (GRCm39)	missense	probably damaging	1.00
R1183:Otog	UTSW	7	45,939,179 (GRCm39)	missense	probably benign	0.41
R1204:Otog	UTSW	7	45,909,335 (GRCm39)	missense	probably benign	0.16
R1301:Otog	UTSW	7	45,939,113 (GRCm39)	missense	probably damaging	1.00
R1344:Otog	UTSW	7	45,924,039 (GRCm39)	missense	probably damaging	1.00
R1384:Otog	UTSW	7	45,923,119 (GRCm39)	splice site	probably benign
R1418:Otog	UTSW	7	45,924,039 (GRCm39)	missense	probably damaging	1.00
R1432:Otog	UTSW	7	45,950,007 (GRCm39)	missense	probably damaging	1.00
R1479:Otog	UTSW	7	45,945,402 (GRCm39)	missense	possibly damaging	0.75
R1521:Otog	UTSW	7	45,908,688 (GRCm39)	missense	possibly damaging	0.71
R1589:Otog	UTSW	7	45,933,332 (GRCm39)	missense	probably benign	0.18
R1671:Otog	UTSW	7	45,911,210 (GRCm39)	missense	probably damaging	1.00
R1773:Otog	UTSW	7	45,937,583 (GRCm39)	missense	probably benign	0.28
R1806:Otog	UTSW	7	45,940,361 (GRCm39)	critical splice acceptor site	probably null
R1843:Otog	UTSW	7	45,895,707 (GRCm39)	missense	probably damaging	1.00
R1873:Otog	UTSW	7	45,918,767 (GRCm39)	missense	probably damaging	1.00
R1923:Otog	UTSW	7	45,895,707 (GRCm39)	missense	probably damaging	1.00
R1927:Otog	UTSW	7	45,895,707 (GRCm39)	missense	probably damaging	1.00
R2008:Otog	UTSW	7	45,913,498 (GRCm39)	missense	probably benign	0.43
R2048:Otog	UTSW	7	45,937,063 (GRCm39)	missense	probably damaging	1.00
R2131:Otog	UTSW	7	45,899,524 (GRCm39)	missense	probably damaging	1.00
R2153:Otog	UTSW	7	45,952,328 (GRCm39)	missense	probably damaging	1.00
R2240:Otog	UTSW	7	45,890,453 (GRCm39)	start codon destroyed	probably null
R2278:Otog	UTSW	7	45,949,468 (GRCm39)	missense	probably damaging	1.00
R2407:Otog	UTSW	7	45,890,964 (GRCm39)	missense	probably benign	0.10
R2424:Otog	UTSW	7	45,947,593 (GRCm39)	nonsense	probably null
R2513:Otog	UTSW	7	45,955,014 (GRCm39)	critical splice donor site	probably null
R2863:Otog	UTSW	7	45,918,730 (GRCm39)	missense	probably damaging	1.00
R3148:Otog	UTSW	7	45,939,593 (GRCm39)	missense	probably damaging	1.00
R3732:Otog	UTSW	7	45,937,792 (GRCm39)	missense	probably benign	0.03
R3732:Otog	UTSW	7	45,937,792 (GRCm39)	missense	probably benign	0.03
R3733:Otog	UTSW	7	45,937,792 (GRCm39)	missense	probably benign	0.03
R3734:Otog	UTSW	7	45,937,792 (GRCm39)	missense	probably benign	0.03
R3855:Otog	UTSW	7	45,923,184 (GRCm39)	missense	possibly damaging	0.65
R3880:Otog	UTSW	7	45,937,445 (GRCm39)	missense	possibly damaging	0.93
R4081:Otog	UTSW	7	45,937,723 (GRCm39)	missense	possibly damaging	0.92
R4349:Otog	UTSW	7	45,923,613 (GRCm39)	missense	probably damaging	0.99
R4382:Otog	UTSW	7	45,939,122 (GRCm39)	missense	probably damaging	1.00
R4392:Otog	UTSW	7	45,934,548 (GRCm39)	missense	probably damaging	0.98
R4520:Otog	UTSW	7	45,890,477 (GRCm39)	unclassified	probably benign
R4569:Otog	UTSW	7	45,959,571 (GRCm39)	missense	probably damaging	1.00
R4580:Otog	UTSW	7	45,937,225 (GRCm39)	missense	possibly damaging	0.78
R4764:Otog	UTSW	7	45,937,943 (GRCm39)	missense	probably benign	0.29
R4910:Otog	UTSW	7	45,947,958 (GRCm39)	missense	probably damaging	1.00
R4910:Otog	UTSW	7	45,913,486 (GRCm39)	missense	probably damaging	1.00
R4913:Otog	UTSW	7	45,913,526 (GRCm39)	missense	probably benign	0.31
R4975:Otog	UTSW	7	45,937,415 (GRCm39)	missense	probably benign	0.00
R4996:Otog	UTSW	7	45,954,934 (GRCm39)	nonsense	probably null
R4996:Otog	UTSW	7	45,948,030 (GRCm39)	missense	possibly damaging	0.51
R5116:Otog	UTSW	7	45,923,191 (GRCm39)	missense	probably benign	0.34
R5138:Otog	UTSW	7	45,899,430 (GRCm39)	missense	possibly damaging	0.61
R5169:Otog	UTSW	7	45,947,572 (GRCm39)	missense	probably benign	0.06
R5239:Otog	UTSW	7	45,936,859 (GRCm39)	missense	probably benign	0.15
R5277:Otog	UTSW	7	45,896,045 (GRCm39)	missense	possibly damaging	0.89
R5287:Otog	UTSW	7	45,918,753 (GRCm39)	missense	probably damaging	0.98
R5299:Otog	UTSW	7	45,938,275 (GRCm39)	missense	probably benign	0.16
R5378:Otog	UTSW	7	45,904,428 (GRCm39)	missense	probably damaging	1.00
R5382:Otog	UTSW	7	45,898,428 (GRCm39)	missense	probably damaging	1.00
R5487:Otog	UTSW	7	45,938,192 (GRCm39)	missense	probably benign	0.27
R5507:Otog	UTSW	7	45,911,123 (GRCm39)	missense	probably damaging	1.00
R5517:Otog	UTSW	7	45,923,995 (GRCm39)	missense	probably damaging	1.00
R5643:Otog	UTSW	7	45,936,871 (GRCm39)	missense	probably damaging	1.00
R5757:Otog	UTSW	7	45,890,545 (GRCm39)	critical splice donor site	probably null
R5910:Otog	UTSW	7	45,948,022 (GRCm39)	missense	possibly damaging	0.94
R6019:Otog	UTSW	7	45,938,374 (GRCm39)	missense	probably benign	0.00
R6150:Otog	UTSW	7	45,913,483 (GRCm39)	missense	possibly damaging	0.82
R6225:Otog	UTSW	7	45,898,458 (GRCm39)	missense	possibly damaging	0.67
R6271:Otog	UTSW	7	45,901,464 (GRCm39)	missense	probably damaging	1.00
R6317:Otog	UTSW	7	45,950,639 (GRCm39)	missense	probably damaging	1.00
R6454:Otog	UTSW	7	45,955,241 (GRCm39)	missense	probably damaging	1.00
R6640:Otog	UTSW	7	45,911,167 (GRCm39)	missense	possibly damaging	0.92
R6753:Otog	UTSW	7	45,898,495 (GRCm39)	missense	probably benign	0.06
R6788:Otog	UTSW	7	45,947,741 (GRCm39)	missense	probably damaging	1.00
R6859:Otog	UTSW	7	45,923,205 (GRCm39)	missense	probably damaging	0.96
R7033:Otog	UTSW	7	45,916,822 (GRCm39)	critical splice donor site	probably null
R7071:Otog	UTSW	7	45,916,747 (GRCm39)	missense	probably damaging	1.00
R7084:Otog	UTSW	7	45,947,990 (GRCm39)	nonsense	probably null
R7116:Otog	UTSW	7	45,947,689 (GRCm39)	missense	probably damaging	0.99
R7202:Otog	UTSW	7	45,937,474 (GRCm39)	missense	probably damaging	0.97
R7365:Otog	UTSW	7	45,947,732 (GRCm39)	missense	probably damaging	1.00
R7468:Otog	UTSW	7	45,913,543 (GRCm39)	missense	probably benign
R7475:Otog	UTSW	7	45,916,700 (GRCm39)	missense	probably damaging	0.99
R7502:Otog	UTSW	7	45,948,039 (GRCm39)	missense	probably damaging	1.00
R7558:Otog	UTSW	7	45,952,584 (GRCm39)	missense	probably damaging	0.99
R7577:Otog	UTSW	7	45,937,279 (GRCm39)	missense	possibly damaging	0.62
R7651:Otog	UTSW	7	45,891,185 (GRCm39)	missense	probably benign	0.00
R7689:Otog	UTSW	7	45,901,480 (GRCm39)	missense	probably damaging	1.00
R7806:Otog	UTSW	7	45,935,200 (GRCm39)	missense	probably benign
R7933:Otog	UTSW	7	45,959,571 (GRCm39)	missense	probably damaging	1.00
R8021:Otog	UTSW	7	45,916,766 (GRCm39)	missense	probably damaging	0.98
R8082:Otog	UTSW	7	45,939,143 (GRCm39)	missense	probably damaging	1.00
R8531:Otog	UTSW	7	45,901,473 (GRCm39)	missense	probably damaging	0.99
R8772:Otog	UTSW	7	45,934,352 (GRCm39)	missense	probably damaging	1.00
R8816:Otog	UTSW	7	45,950,905 (GRCm39)	missense	possibly damaging	0.92
R8842:Otog	UTSW	7	45,895,948 (GRCm39)	missense	probably damaging	1.00
R8987:Otog	UTSW	7	45,936,878 (GRCm39)	missense	probably benign	0.43
R8988:Otog	UTSW	7	45,959,571 (GRCm39)	missense	probably damaging	1.00
R9010:Otog	UTSW	7	45,949,894 (GRCm39)	missense	probably benign	0.00
R9025:Otog	UTSW	7	45,937,520 (GRCm39)	missense	probably benign	0.13
R9131:Otog	UTSW	7	45,952,597 (GRCm39)	nonsense	probably null
R9179:Otog	UTSW	7	45,937,885 (GRCm39)	missense	possibly damaging	0.65
R9334:Otog	UTSW	7	45,909,353 (GRCm39)	missense	possibly damaging	0.95
R9365:Otog	UTSW	7	45,920,688 (GRCm39)	missense	probably damaging	1.00
R9408:Otog	UTSW	7	45,916,721 (GRCm39)	missense	possibly damaging	0.79
R9418:Otog	UTSW	7	45,938,024 (GRCm39)	missense	probably benign	0.41
R9465:Otog	UTSW	7	45,955,299 (GRCm39)	missense	possibly damaging	0.80
R9496:Otog	UTSW	7	45,890,505 (GRCm39)	missense	unknown
R9632:Otog	UTSW	7	45,915,143 (GRCm39)	missense	probably benign	0.27
R9656:Otog	UTSW	7	45,959,567 (GRCm39)	missense	probably damaging	1.00
RF024:Otog	UTSW	7	45,937,093 (GRCm39)	missense	probably damaging	1.00
X0062:Otog	UTSW	7	45,909,345 (GRCm39)	missense	probably damaging	1.00
Z1177:Otog	UTSW	7	45,939,164 (GRCm39)	missense	probably damaging	1.00
Z1177:Otog	UTSW	7	45,923,962 (GRCm39)	missense	probably damaging	1.00
Z1177:Otog	UTSW	7	45,912,276 (GRCm39)	missense	possibly damaging	0.80
Z1177:Otog	UTSW	7	45,959,409 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGGCTTGTTCCAGAAGGGTC -3'
(R):5'- CTCACAGAGTTCAGAGGCCATG -3'

Sequencing Primer
(F):5'- GGTAGGTAACACCGTAAGCTCTG -3'
(R):5'- CCATGAGGGAAGGAAATTTGTC -3'

Posted On

2015-10-08