Mutagenetix > Incidental Mutation

Incidental Mutation 'R4629:Vmn2r81'

349058

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r81

Ensembl Gene

ENSMUSG00000055515

Gene Name

vomeronasal 2, receptor 81

Synonyms

pheromone recepter, V2rf2, EC1-VR2

MMRRC Submission

041894-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.056) question?

Stock #

R4629 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

79083611-79130369 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 79103276 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Aspartic acid at position 156 (E156D)

Ref Sequence

ENSEMBL: ENSMUSP00000020547 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020547]

AlphaFold

Q80Z09

Predicted Effect

probably damaging
Transcript: ENSMUST00000020547
AA Change: E156D

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000020547
Gene: ENSMUSG00000055515
AA Change: E156D

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	79	474	7.7e-37	PFAM
Pfam:NCD3G	517	570	8.9e-21	PFAM
Pfam:7tm_3	603	838	6.1e-50	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.4%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit vomeronasal sensory neuron axons that spread out diffusely over the posterior accessory olfactory bulb and extend abnormally into the anterior olfactory bulb. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 76 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921528I07Rik	T	C	9: 114,108,419 (GRCm39)		noncoding transcript	Het
Abca6	T	C	11: 110,121,375 (GRCm39)		probably null	Het
Adam22	A	G	5: 8,282,663 (GRCm39)	S111P	possibly damaging	Het
Adamts18	C	T	8: 114,499,800 (GRCm39)	W371*	probably null	Het
Akr1c13	G	T	13: 4,247,869 (GRCm39)	V214F	probably damaging	Het
Alg10b	C	T	15: 90,111,948 (GRCm39)	A264V	probably benign	Het
Angpt1	T	A	15: 42,301,796 (GRCm39)	Y404F	probably benign	Het
Apol7c	A	G	15: 77,410,595 (GRCm39)	F117S	probably damaging	Het
Asic5	T	A	3: 81,913,811 (GRCm39)	Y162N	probably damaging	Het
Cacna2d2	A	T	9: 107,404,521 (GRCm39)	E1104V	probably damaging	Het
Cad	G	A	5: 31,227,639 (GRCm39)	V1263I	probably damaging	Het
Cdc20	T	C	4: 118,290,761 (GRCm39)	E413G	probably damaging	Het
Cfap46	A	T	7: 139,260,843 (GRCm39)	L85Q	probably damaging	Het
Cnot6l	A	G	5: 96,225,070 (GRCm39)	V541A	probably benign	Het
Cubn	T	A	2: 13,318,790 (GRCm39)		probably null	Het
Cx3cr1	T	C	9: 119,880,730 (GRCm39)	N224S	probably damaging	Het
Fam98c	T	C	7: 28,854,693 (GRCm39)	T49A	possibly damaging	Het
Fez2	C	T	17: 78,710,183 (GRCm39)	S202N	probably benign	Het
Fras1	A	G	5: 96,924,593 (GRCm39)	N3678S	probably benign	Het
Glyr1	A	G	16: 4,854,907 (GRCm39)	V57A	possibly damaging	Het
Gm5617	T	A	9: 48,407,187 (GRCm39)	L107Q	possibly damaging	Het
Gpnmb	A	G	6: 49,027,994 (GRCm39)	D401G	possibly damaging	Het
Gtpbp6	C	A	5: 110,254,774 (GRCm39)	V100L	possibly damaging	Het
Gucy1a1	A	G	3: 82,004,931 (GRCm39)	V618A	probably damaging	Het
H6pd	A	T	4: 150,080,803 (GRCm39)	M14K	probably benign	Het
Hectd4	A	G	5: 121,435,266 (GRCm39)	M993V	probably benign	Het
Kat14	A	G	2: 144,246,140 (GRCm39)		probably benign	Het
Kctd21	A	G	7: 96,996,782 (GRCm39)	D85G	probably damaging	Het
Kera	A	G	10: 97,445,493 (GRCm39)	N284S	probably benign	Het
Krt1	C	T	15: 101,754,622 (GRCm39)	G543S	unknown	Het
Lama1	A	G	17: 68,112,355 (GRCm39)		probably null	Het
Lgr6	T	C	1: 135,032,670 (GRCm39)	Y70C	probably damaging	Het
Lipg	T	A	18: 75,081,107 (GRCm39)	K325*	probably null	Het
Lrrc43	T	C	5: 123,637,583 (GRCm39)	L250P	probably damaging	Het
Lyz1	C	T	10: 117,127,041 (GRCm39)	R65H	probably benign	Het
Marchf10	T	A	11: 105,280,664 (GRCm39)	L540F	probably benign	Het
Maz	G	T	7: 126,624,519 (GRCm39)	H334N	possibly damaging	Het
Myh1	A	G	11: 67,100,119 (GRCm39)	K646R	probably benign	Het
Nf2	A	T	11: 4,798,915 (GRCm39)	V24E	probably damaging	Het
Nup210l	T	C	3: 90,075,182 (GRCm39)	S831P	probably benign	Het
Nup210l	C	T	3: 90,098,181 (GRCm39)	R1378*	probably null	Het
Or13a17	T	A	7: 140,271,291 (GRCm39)	S158T	probably benign	Het
Orc5	G	T	5: 22,753,003 (GRCm39)	F10L	probably benign	Het
Palb2	A	C	7: 121,727,189 (GRCm39)	I227S	possibly damaging	Het
Pate3	A	T	9: 35,557,453 (GRCm39)	C68S	probably damaging	Het
Pcdha12	T	A	18: 37,154,926 (GRCm39)	N548K	probably damaging	Het
Prdm10	C	T	9: 31,248,612 (GRCm39)	Q345*	probably null	Het
Ptx4	A	T	17: 25,341,737 (GRCm39)	N71Y	probably damaging	Het
Rab42	C	T	4: 132,030,548 (GRCm39)	R34Q	probably benign	Het
Rergl	A	G	6: 139,478,850 (GRCm39)	V8A	probably damaging	Het
Rmi1	A	G	13: 58,556,950 (GRCm39)	R400G	probably benign	Het
Rpia	A	G	6: 70,743,578 (GRCm39)	M291T	possibly damaging	Het
Rplp0	G	A	5: 115,699,482 (GRCm39)		probably null	Het
Rrp12	A	T	19: 41,871,955 (GRCm39)	I443N	probably benign	Het
Rsf1	GGCG	GGCGACGGCCGCG	7: 97,229,113 (GRCm39)		probably benign	Het
Rsf1	CG	CGACGGCGGTG	7: 97,229,115 (GRCm39)		probably benign	Het
Saxo1	T	C	4: 86,406,064 (GRCm39)	Y45C	probably damaging	Het
Sec24a	A	T	11: 51,612,640 (GRCm39)		probably null	Het
Setdb2	A	G	14: 59,646,808 (GRCm39)	V585A	probably benign	Het
Sik1	T	C	17: 32,068,581 (GRCm39)	E347G	probably benign	Het
Srbd1	T	C	17: 86,428,100 (GRCm39)	T378A	probably damaging	Het
Tacr1	A	G	6: 82,380,861 (GRCm39)	T91A	probably benign	Het
Tas2r135	T	A	6: 42,383,160 (GRCm39)	M233K	probably benign	Het
Tfg	A	T	16: 56,533,039 (GRCm39)	M40K	probably damaging	Het
Tmem35b	C	T	4: 127,022,796 (GRCm39)	P133S	probably benign	Het
Tmtc2	A	T	10: 105,139,511 (GRCm39)	S672T	probably benign	Het
Trpv3	A	T	11: 73,172,615 (GRCm39)	K253N	probably damaging	Het
Ube2c	A	G	2: 164,614,093 (GRCm39)	N143S	possibly damaging	Het
Unc13a	A	G	8: 72,106,097 (GRCm39)	M669T	possibly damaging	Het
Vmn1r61	T	A	7: 5,614,249 (GRCm39)	I22F	probably benign	Het
Vmn2r26	A	T	6: 124,038,150 (GRCm39)	Q575L	possibly damaging	Het
Vwa3a	T	A	7: 120,392,598 (GRCm39)	N812K	probably benign	Het
Wasl	T	C	6: 24,637,680 (GRCm39)	R71G	probably damaging	Het
Wdfy4	A	T	14: 32,824,515 (GRCm39)	N1301K	probably damaging	Het
Zfp827	G	A	8: 79,787,011 (GRCm39)	R59Q	probably damaging	Het
Zfp87	A	G	13: 74,520,512 (GRCm39)	C189R	probably damaging	Het

Other mutations in Vmn2r81

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01111:Vmn2r81	APN	10	79,083,831 (GRCm39)	missense	probably benign	0.20
IGL02255:Vmn2r81	APN	10	79,083,806 (GRCm39)	nonsense	probably null
IGL02901:Vmn2r81	APN	10	79,106,564 (GRCm39)	missense	probably damaging	0.98
R0383:Vmn2r81	UTSW	10	79,129,281 (GRCm39)	missense	possibly damaging	0.95
R0555:Vmn2r81	UTSW	10	79,129,283 (GRCm39)	missense	probably damaging	1.00
R0715:Vmn2r81	UTSW	10	79,104,434 (GRCm39)	missense	probably damaging	1.00
R0967:Vmn2r81	UTSW	10	79,083,857 (GRCm39)	splice site	probably benign
R1438:Vmn2r81	UTSW	10	79,129,691 (GRCm39)	missense	probably benign	0.00
R1468:Vmn2r81	UTSW	10	79,129,496 (GRCm39)	missense	probably damaging	1.00
R1468:Vmn2r81	UTSW	10	79,129,496 (GRCm39)	missense	probably damaging	1.00
R1589:Vmn2r81	UTSW	10	79,128,858 (GRCm39)	missense	probably damaging	0.99
R1671:Vmn2r81	UTSW	10	79,103,265 (GRCm39)	missense	probably benign	0.04
R1672:Vmn2r81	UTSW	10	79,104,112 (GRCm39)	missense	probably damaging	0.99
R1728:Vmn2r81	UTSW	10	79,106,489 (GRCm39)	missense	probably benign	0.08
R1784:Vmn2r81	UTSW	10	79,106,489 (GRCm39)	missense	probably benign	0.08
R1931:Vmn2r81	UTSW	10	79,129,328 (GRCm39)	missense	probably damaging	0.98
R1934:Vmn2r81	UTSW	10	79,083,628 (GRCm39)	start codon destroyed	probably null	0.71
R1944:Vmn2r81	UTSW	10	79,129,571 (GRCm39)	missense	probably damaging	1.00
R2102:Vmn2r81	UTSW	10	79,129,334 (GRCm39)	missense	probably damaging	0.97
R2190:Vmn2r81	UTSW	10	79,104,085 (GRCm39)	missense	possibly damaging	0.67
R2392:Vmn2r81	UTSW	10	79,110,516 (GRCm39)	missense	probably damaging	1.00
R3770:Vmn2r81	UTSW	10	79,106,434 (GRCm39)	missense	probably damaging	1.00
R4551:Vmn2r81	UTSW	10	79,104,241 (GRCm39)	missense	possibly damaging	0.95
R4691:Vmn2r81	UTSW	10	79,129,211 (GRCm39)	nonsense	probably null
R4829:Vmn2r81	UTSW	10	79,083,635 (GRCm39)	missense	possibly damaging	0.48
R4914:Vmn2r81	UTSW	10	79,106,357 (GRCm39)	missense	probably null	1.00
R4996:Vmn2r81	UTSW	10	79,129,247 (GRCm39)	missense	probably benign	0.04
R5242:Vmn2r81	UTSW	10	79,129,309 (GRCm39)	nonsense	probably null
R5253:Vmn2r81	UTSW	10	79,083,820 (GRCm39)	missense	probably benign	0.00
R5925:Vmn2r81	UTSW	10	79,083,637 (GRCm39)	missense	probably damaging	1.00
R6169:Vmn2r81	UTSW	10	79,104,382 (GRCm39)	missense	probably benign
R6270:Vmn2r81	UTSW	10	79,129,649 (GRCm39)	missense	probably benign
R6525:Vmn2r81	UTSW	10	79,129,560 (GRCm39)	missense	probably benign	0.11
R6813:Vmn2r81	UTSW	10	79,104,439 (GRCm39)	missense	probably benign	0.00
R6886:Vmn2r81	UTSW	10	79,103,988 (GRCm39)	missense	possibly damaging	0.90
R7200:Vmn2r81	UTSW	10	79,106,570 (GRCm39)	critical splice donor site	probably null
R7350:Vmn2r81	UTSW	10	79,104,219 (GRCm39)	missense	probably benign	0.03
R7504:Vmn2r81	UTSW	10	79,104,166 (GRCm39)	missense	probably benign
R7832:Vmn2r81	UTSW	10	79,129,664 (GRCm39)	missense	probably damaging	1.00
R8130:Vmn2r81	UTSW	10	79,110,538 (GRCm39)	missense	possibly damaging	0.95
R8362:Vmn2r81	UTSW	10	79,103,283 (GRCm39)	missense	probably benign	0.17
R8407:Vmn2r81	UTSW	10	79,104,028 (GRCm39)	missense	possibly damaging	0.92
R8540:Vmn2r81	UTSW	10	79,129,065 (GRCm39)	missense	probably damaging	0.99
R8558:Vmn2r81	UTSW	10	79,106,467 (GRCm39)	missense	possibly damaging	0.94
R8700:Vmn2r81	UTSW	10	79,129,517 (GRCm39)	missense	probably damaging	1.00
R8779:Vmn2r81	UTSW	10	79,103,218 (GRCm39)	missense	possibly damaging	0.78
R8987:Vmn2r81	UTSW	10	79,129,704 (GRCm39)	missense	probably damaging	1.00
R9001:Vmn2r81	UTSW	10	79,104,104 (GRCm39)	missense	possibly damaging	0.73
R9055:Vmn2r81	UTSW	10	79,110,441 (GRCm39)	missense	probably benign
R9145:Vmn2r81	UTSW	10	79,104,028 (GRCm39)	missense	possibly damaging	0.92
R9151:Vmn2r81	UTSW	10	79,103,905 (GRCm39)	missense
R9253:Vmn2r81	UTSW	10	79,129,582 (GRCm39)	missense	probably damaging	1.00
R9255:Vmn2r81	UTSW	10	79,103,166 (GRCm39)	missense	possibly damaging	0.77
R9370:Vmn2r81	UTSW	10	79,104,424 (GRCm39)	missense	probably damaging	1.00
R9588:Vmn2r81	UTSW	10	79,129,205 (GRCm39)	missense	possibly damaging	0.46
X0067:Vmn2r81	UTSW	10	79,128,877 (GRCm39)	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- AACACCTCTCTTGGACTTGAG -3'
(R):5'- ATGGAGAACAAGAATTGCCATTCAC -3'

Sequencing Primer
(F):5'- AGATTTATAATCTGCCAGCTATTGG -3'
(R):5'- TCCAGCCTGGGTCAAGTTAATAC -3'

Posted On

2015-10-08