Mutagenetix > Incidental Mutation

Incidental Mutation 'R4629:Wdfy4'

349071

Institutional Source

Beutler Lab

Gene Symbol

Wdfy4

Ensembl Gene

ENSMUSG00000051506

Gene Name

WD repeat and FYVE domain containing 4

Synonyms

MMRRC Submission

041894-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4629 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

32959547-33185508 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 33102558 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Lysine at position 1301 (N1301K)

Ref Sequence

ENSEMBL: ENSMUSP00000117068 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061753] [ENSMUST00000130509]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000061753
AA Change: N1256K

PolyPhen 2 Score 0.959 (Sensitivity: 0.78; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000057556
Gene: ENSMUSG00000051506
AA Change: N1256K

Domain	Start	End	E-Value	Type
low complexity region	69	77	N/A	INTRINSIC
low complexity region	204	222	N/A	INTRINSIC
low complexity region	508	522	N/A	INTRINSIC
low complexity region	618	632	N/A	INTRINSIC
low complexity region	644	661	N/A	INTRINSIC
low complexity region	1585	1604	N/A	INTRINSIC
low complexity region	1899	1909	N/A	INTRINSIC
Pfam:PH_BEACH	2237	2348	1.2e-9	PFAM
Beach	2378	2660	3.69e-196	SMART
WD40	2761	2801	1.98e1	SMART
WD40	2811	2850	5.18e-7	SMART
WD40	2853	2891	9.94e-1	SMART
WD40	2893	2940	3.17e-2	SMART
WD40	2986	3021	3.31e0	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000130509
AA Change: N1301K

PolyPhen 2 Score 0.969 (Sensitivity: 0.77; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000117068
Gene: ENSMUSG00000051506
AA Change: N1301K

Domain	Start	End	E-Value	Type
low complexity region	69	77	N/A	INTRINSIC
low complexity region	204	222	N/A	INTRINSIC
low complexity region	508	522	N/A	INTRINSIC
low complexity region	618	632	N/A	INTRINSIC
low complexity region	644	661	N/A	INTRINSIC
low complexity region	1596	1615	N/A	INTRINSIC
low complexity region	1795	1819	N/A	INTRINSIC
low complexity region	2019	2029	N/A	INTRINSIC
Pfam:PH_BEACH	2362	2473	1.2e-9	PFAM
Beach	2503	2785	3.69e-196	SMART
WD40	2886	2926	1.98e1	SMART
WD40	2936	2975	5.18e-7	SMART
WD40	2978	3016	9.94e-1	SMART
WD40	3018	3065	3.17e-2	SMART
WD40	3111	3146	3.31e0	SMART

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 76 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921528I07Rik	T	C	9: 114,279,351 (GRCm38)		noncoding transcript	Het
Abca6	T	C	11: 110,230,549 (GRCm38)		probably null	Het
Adam22	A	G	5: 8,232,663 (GRCm38)	S111P	possibly damaging	Het
Adamts18	C	T	8: 113,773,168 (GRCm38)	W371*	probably null	Het
Akr1c13	G	T	13: 4,197,870 (GRCm38)	V214F	probably damaging	Het
Alg10b	C	T	15: 90,227,745 (GRCm38)	A264V	probably benign	Het
Angpt1	T	A	15: 42,438,400 (GRCm38)	Y404F	probably benign	Het
Apol7c	A	G	15: 77,526,395 (GRCm38)	F117S	probably damaging	Het
Asic5	T	A	3: 82,006,504 (GRCm38)	Y162N	probably damaging	Het
Cacna2d2	A	T	9: 107,527,322 (GRCm38)	E1104V	probably damaging	Het
Cad	G	A	5: 31,070,295 (GRCm38)	V1263I	probably damaging	Het
Cdc20	T	C	4: 118,433,564 (GRCm38)	E413G	probably damaging	Het
Cfap46	A	T	7: 139,680,927 (GRCm38)	L85Q	probably damaging	Het
Cnot6l	A	G	5: 96,077,211 (GRCm38)	V541A	probably benign	Het
Cubn	T	A	2: 13,313,979 (GRCm38)		probably null	Het
Cx3cr1	T	C	9: 120,051,664 (GRCm38)	N224S	probably damaging	Het
Fam98c	T	C	7: 29,155,268 (GRCm38)	T49A	possibly damaging	Het
Fez2	C	T	17: 78,402,754 (GRCm38)	S202N	probably benign	Het
Fras1	A	G	5: 96,776,734 (GRCm38)	N3678S	probably benign	Het
Glyr1	A	G	16: 5,037,043 (GRCm38)	V57A	possibly damaging	Het
Gm5617	T	A	9: 48,495,887 (GRCm38)	L107Q	possibly damaging	Het
Gpnmb	A	G	6: 49,051,060 (GRCm38)	D401G	possibly damaging	Het
Gtpbp6	C	A	5: 110,106,908 (GRCm38)	V100L	possibly damaging	Het
Gucy1a1	A	G	3: 82,097,624 (GRCm38)	V618A	probably damaging	Het
H6pd	A	T	4: 149,996,346 (GRCm38)	M14K	probably benign	Het
Hectd4	A	G	5: 121,297,203 (GRCm38)	M993V	probably benign	Het
Kat14	A	G	2: 144,404,220 (GRCm38)		probably benign	Het
Kctd21	A	G	7: 97,347,575 (GRCm38)	D85G	probably damaging	Het
Kera	A	G	10: 97,609,631 (GRCm38)	N284S	probably benign	Het
Krt1	C	T	15: 101,846,187 (GRCm38)	G543S	unknown	Het
Lama1	A	G	17: 67,805,360 (GRCm38)		probably null	Het
Lgr6	T	C	1: 135,104,932 (GRCm38)	Y70C	probably damaging	Het
Lipg	T	A	18: 74,948,036 (GRCm38)	K325*	probably null	Het
Lrrc43	T	C	5: 123,499,520 (GRCm38)	L250P	probably damaging	Het
Lyz1	C	T	10: 117,291,136 (GRCm38)	R65H	probably benign	Het
March10	T	A	11: 105,389,838 (GRCm38)	L540F	probably benign	Het
Maz	G	T	7: 127,025,347 (GRCm38)	H334N	possibly damaging	Het
Myh1	A	G	11: 67,209,293 (GRCm38)	K646R	probably benign	Het
Nf2	A	T	11: 4,848,915 (GRCm38)	V24E	probably damaging	Het
Nup210l	T	C	3: 90,167,875 (GRCm38)	S831P	probably benign	Het
Nup210l	C	T	3: 90,190,874 (GRCm38)	R1378*	probably null	Het
Olfr45	T	A	7: 140,691,378 (GRCm38)	S158T	probably benign	Het
Orc5	G	T	5: 22,548,005 (GRCm38)	F10L	probably benign	Het
Palb2	A	C	7: 122,127,966 (GRCm38)	I227S	possibly damaging	Het
Pate3	A	T	9: 35,646,157 (GRCm38)	C68S	probably damaging	Het
Pcdha12	T	A	18: 37,021,873 (GRCm38)	N548K	probably damaging	Het
Prdm10	C	T	9: 31,337,316 (GRCm38)	Q345*	probably null	Het
Ptx4	A	T	17: 25,122,763 (GRCm38)	N71Y	probably damaging	Het
Rab42	C	T	4: 132,303,237 (GRCm38)	R34Q	probably benign	Het
Rergl	A	G	6: 139,501,852 (GRCm38)	V8A	probably damaging	Het
Rmi1	A	G	13: 58,409,136 (GRCm38)	R400G	probably benign	Het
Rpia	A	G	6: 70,766,594 (GRCm38)	M291T	possibly damaging	Het
Rplp0	G	A	5: 115,561,423 (GRCm38)		probably null	Het
Rrp12	A	T	19: 41,883,516 (GRCm38)	I443N	probably benign	Het
Rsf1	GGCG	GGCGACGGCCGCG	7: 97,579,906 (GRCm38)		probably benign	Het
Rsf1	CG	CGACGGCGGTG	7: 97,579,908 (GRCm38)		probably benign	Het
Saxo1	T	C	4: 86,487,827 (GRCm38)	Y45C	probably damaging	Het
Sec24a	A	T	11: 51,721,813 (GRCm38)		probably null	Het
Setdb2	A	G	14: 59,409,359 (GRCm38)	V585A	probably benign	Het
Sik1	T	C	17: 31,849,607 (GRCm38)	E347G	probably benign	Het
Srbd1	T	C	17: 86,120,672 (GRCm38)	T378A	probably damaging	Het
Tacr1	A	G	6: 82,403,880 (GRCm38)	T91A	probably benign	Het
Tas2r135	T	A	6: 42,406,226 (GRCm38)	M233K	probably benign	Het
Tfg	A	T	16: 56,712,676 (GRCm38)	M40K	probably damaging	Het
Tmem35b	C	T	4: 127,129,003 (GRCm38)	P133S	probably benign	Het
Tmtc2	A	T	10: 105,303,650 (GRCm38)	S672T	probably benign	Het
Trpv3	A	T	11: 73,281,789 (GRCm38)	K253N	probably damaging	Het
Ube2c	A	G	2: 164,772,173 (GRCm38)	N143S	possibly damaging	Het
Unc13a	A	G	8: 71,653,453 (GRCm38)	M669T	possibly damaging	Het
Vmn1r61	T	A	7: 5,611,250 (GRCm38)	I22F	probably benign	Het
Vmn2r26	A	T	6: 124,061,191 (GRCm38)	Q575L	possibly damaging	Het
Vmn2r81	A	T	10: 79,267,442 (GRCm38)	E156D	probably damaging	Het
Vwa3a	T	A	7: 120,793,375 (GRCm38)	N812K	probably benign	Het
Wasl	T	C	6: 24,637,681 (GRCm38)	R71G	probably damaging	Het
Zfp72	A	G	13: 74,372,393 (GRCm38)	C189R	probably damaging	Het
Zfp827	G	A	8: 79,060,382 (GRCm38)	R59Q	probably damaging	Het

Other mutations in Wdfy4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00231:Wdfy4	APN	14	33,102,539 (GRCm38)	missense	possibly damaging	0.93
IGL01116:Wdfy4	APN	14	32,959,977 (GRCm38)	missense	probably damaging	1.00
IGL01449:Wdfy4	APN	14	33,104,037 (GRCm38)	missense	probably damaging	0.99
IGL01567:Wdfy4	APN	14	33,151,661 (GRCm38)	missense	probably benign	0.01
IGL01700:Wdfy4	APN	14	33,020,238 (GRCm38)	splice site	probably benign
IGL01931:Wdfy4	APN	14	33,155,753 (GRCm38)	missense	probably damaging	1.00
IGL01981:Wdfy4	APN	14	33,133,716 (GRCm38)	missense	probably damaging	1.00
IGL01988:Wdfy4	APN	14	33,076,480 (GRCm38)	missense	possibly damaging	0.75
IGL02026:Wdfy4	APN	14	33,093,300 (GRCm38)	missense	probably damaging	1.00
IGL02066:Wdfy4	APN	14	33,149,566 (GRCm38)	missense	probably benign
IGL02468:Wdfy4	APN	14	32,966,432 (GRCm38)	missense	probably benign	0.01
IGL02512:Wdfy4	APN	14	33,042,491 (GRCm38)	missense	probably benign	0.01
IGL02597:Wdfy4	APN	14	33,090,861 (GRCm38)	nonsense	probably null
IGL02752:Wdfy4	APN	14	33,076,326 (GRCm38)	missense	probably damaging	1.00
IGL02792:Wdfy4	APN	14	33,095,305 (GRCm38)	missense	probably benign	0.01
IGL02826:Wdfy4	APN	14	32,971,750 (GRCm38)	missense	possibly damaging	0.47
IGL02903:Wdfy4	APN	14	33,109,650 (GRCm38)	missense	probably damaging	1.00
IGL02955:Wdfy4	APN	14	33,076,284 (GRCm38)	missense	probably damaging	1.00
IGL03031:Wdfy4	APN	14	33,140,651 (GRCm38)	missense	probably damaging	1.00
IGL03102:Wdfy4	APN	14	32,966,435 (GRCm38)	missense	probably damaging	1.00
IGL03123:Wdfy4	APN	14	33,162,870 (GRCm38)	missense	probably benign	0.01
IGL03198:Wdfy4	APN	14	33,125,887 (GRCm38)	missense	probably damaging	1.00
IGL03250:Wdfy4	APN	14	32,977,167 (GRCm38)	missense	probably damaging	0.99
IGL03277:Wdfy4	APN	14	33,068,904 (GRCm38)	missense	probably benign	0.01
IGL03398:Wdfy4	APN	14	33,047,290 (GRCm38)	missense	probably benign	0.14
dodgers	UTSW	14	32,977,106 (GRCm38)	nonsense	probably null
Dollar	UTSW	14	33,020,311 (GRCm38)	missense	probably damaging	1.00
Giants	UTSW	14	33,070,618 (GRCm38)	nonsense	probably null
gigantea	UTSW	14	32,974,154 (GRCm38)	critical splice donor site	probably null
kings_canyon	UTSW	14	33,109,519 (GRCm38)	nonsense	probably null
moro	UTSW	14	32,964,626 (GRCm38)	splice site	probably null
popped	UTSW	14	32,966,399 (GRCm38)	missense	probably damaging	0.99
sequoia	UTSW	14	33,100,903 (GRCm38)	critical splice donor site	probably null
Sherman	UTSW	14	33,095,951 (GRCm38)	missense	possibly damaging	0.89
stretched	UTSW	14	33,073,535 (GRCm38)	nonsense	probably null
watchtower	UTSW	14	33,083,639 (GRCm38)	critical splice donor site	probably null
R0014:Wdfy4	UTSW	14	33,107,173 (GRCm38)	missense	possibly damaging	0.72
R0067:Wdfy4	UTSW	14	33,162,751 (GRCm38)	missense	probably null	1.00
R0085:Wdfy4	UTSW	14	33,078,243 (GRCm38)	missense	possibly damaging	0.81
R0277:Wdfy4	UTSW	14	33,083,785 (GRCm38)	missense	possibly damaging	0.83
R0436:Wdfy4	UTSW	14	33,083,812 (GRCm38)	splice site	probably benign
R0496:Wdfy4	UTSW	14	33,140,738 (GRCm38)	splice site	probably benign
R0514:Wdfy4	UTSW	14	33,080,775 (GRCm38)	missense	probably benign	0.22
R0548:Wdfy4	UTSW	14	33,042,621 (GRCm38)	missense	probably benign
R0590:Wdfy4	UTSW	14	33,041,174 (GRCm38)	missense	probably benign	0.09
R0647:Wdfy4	UTSW	14	33,109,699 (GRCm38)	missense	possibly damaging	0.96
R0766:Wdfy4	UTSW	14	33,140,612 (GRCm38)	missense	probably damaging	1.00
R0981:Wdfy4	UTSW	14	33,147,092 (GRCm38)	missense	probably benign	0.03
R1024:Wdfy4	UTSW	14	33,079,966 (GRCm38)	missense	possibly damaging	0.81
R1113:Wdfy4	UTSW	14	32,971,738 (GRCm38)	missense	possibly damaging	0.47
R1252:Wdfy4	UTSW	14	32,971,772 (GRCm38)	splice site	probably null
R1415:Wdfy4	UTSW	14	33,041,180 (GRCm38)	missense	possibly damaging	0.60
R1475:Wdfy4	UTSW	14	33,108,688 (GRCm38)	missense	probably benign	0.14
R1483:Wdfy4	UTSW	14	33,100,966 (GRCm38)	missense	probably benign	0.41
R1490:Wdfy4	UTSW	14	33,152,538 (GRCm38)	critical splice donor site	probably null
R1512:Wdfy4	UTSW	14	32,960,808 (GRCm38)	missense	probably damaging	0.98
R1615:Wdfy4	UTSW	14	33,042,512 (GRCm38)	missense	probably damaging	1.00
R1628:Wdfy4	UTSW	14	32,959,961 (GRCm38)	missense	probably damaging	1.00
R1643:Wdfy4	UTSW	14	33,073,585 (GRCm38)	critical splice acceptor site	probably null
R1729:Wdfy4	UTSW	14	33,096,005 (GRCm38)	missense	possibly damaging	0.85
R1859:Wdfy4	UTSW	14	33,103,983 (GRCm38)	missense	probably damaging	0.99
R1933:Wdfy4	UTSW	14	33,133,344 (GRCm38)	missense	probably benign	0.08
R1957:Wdfy4	UTSW	14	32,971,684 (GRCm38)	missense	probably damaging	1.00
R1968:Wdfy4	UTSW	14	33,106,044 (GRCm38)	missense	possibly damaging	0.95
R2032:Wdfy4	UTSW	14	33,146,989 (GRCm38)	missense	probably benign	0.11
R2241:Wdfy4	UTSW	14	33,073,511 (GRCm38)	missense	possibly damaging	0.81
R2391:Wdfy4	UTSW	14	33,162,807 (GRCm38)	missense	possibly damaging	0.92
R2888:Wdfy4	UTSW	14	33,109,519 (GRCm38)	nonsense	probably null
R2889:Wdfy4	UTSW	14	33,109,519 (GRCm38)	nonsense	probably null
R3114:Wdfy4	UTSW	14	33,089,903 (GRCm38)	missense	probably damaging	0.97
R3757:Wdfy4	UTSW	14	33,023,374 (GRCm38)	missense	probably benign	0.17
R3758:Wdfy4	UTSW	14	33,023,374 (GRCm38)	missense	probably benign	0.17
R3797:Wdfy4	UTSW	14	33,140,645 (GRCm38)	missense	probably damaging	1.00
R3890:Wdfy4	UTSW	14	33,047,280 (GRCm38)	missense	probably damaging	1.00
R3892:Wdfy4	UTSW	14	33,047,280 (GRCm38)	missense	probably damaging	1.00
R3945:Wdfy4	UTSW	14	32,966,395 (GRCm38)	missense	probably damaging	0.99
R4011:Wdfy4	UTSW	14	33,102,680 (GRCm38)	splice site	probably benign
R4091:Wdfy4	UTSW	14	33,125,880 (GRCm38)	missense	possibly damaging	0.93
R4449:Wdfy4	UTSW	14	33,096,083 (GRCm38)	missense	probably damaging	1.00
R4585:Wdfy4	UTSW	14	33,087,955 (GRCm38)	missense	possibly damaging	0.89
R4628:Wdfy4	UTSW	14	33,102,558 (GRCm38)	missense	probably damaging	0.97
R4655:Wdfy4	UTSW	14	32,989,936 (GRCm38)	missense	probably damaging	0.98
R4689:Wdfy4	UTSW	14	33,109,548 (GRCm38)	missense	possibly damaging	0.88
R4718:Wdfy4	UTSW	14	33,145,316 (GRCm38)	missense	probably benign	0.03
R4862:Wdfy4	UTSW	14	33,100,903 (GRCm38)	critical splice donor site	probably null
R4884:Wdfy4	UTSW	14	32,988,895 (GRCm38)	nonsense	probably null
R4894:Wdfy4	UTSW	14	33,155,760 (GRCm38)	missense	probably benign	0.03
R4929:Wdfy4	UTSW	14	33,047,256 (GRCm38)	missense	possibly damaging	0.90
R4932:Wdfy4	UTSW	14	33,029,013 (GRCm38)	missense	probably damaging	1.00
R5014:Wdfy4	UTSW	14	33,100,940 (GRCm38)	missense	probably benign	0.02
R5020:Wdfy4	UTSW	14	33,079,935 (GRCm38)	missense	probably damaging	1.00
R5049:Wdfy4	UTSW	14	33,152,670 (GRCm38)	missense	possibly damaging	0.78
R5276:Wdfy4	UTSW	14	33,047,275 (GRCm38)	missense	probably damaging	1.00
R5318:Wdfy4	UTSW	14	33,078,343 (GRCm38)	missense	possibly damaging	0.95
R5338:Wdfy4	UTSW	14	33,090,866 (GRCm38)	missense	probably damaging	1.00
R5349:Wdfy4	UTSW	14	32,988,899 (GRCm38)	missense	probably damaging	1.00
R5411:Wdfy4	UTSW	14	32,960,002 (GRCm38)	missense	probably damaging	1.00
R5435:Wdfy4	UTSW	14	33,020,311 (GRCm38)	missense	probably damaging	1.00
R5463:Wdfy4	UTSW	14	33,151,732 (GRCm38)	missense	probably benign	0.17
R5591:Wdfy4	UTSW	14	33,107,130 (GRCm38)	missense	probably benign	0.09
R5598:Wdfy4	UTSW	14	33,133,497 (GRCm38)	missense	probably damaging	1.00
R5654:Wdfy4	UTSW	14	33,107,618 (GRCm38)	splice site	probably null
R5890:Wdfy4	UTSW	14	33,102,577 (GRCm38)	missense	possibly damaging	0.91
R5894:Wdfy4	UTSW	14	33,133,360 (GRCm38)	missense	possibly damaging	0.86
R5964:Wdfy4	UTSW	14	33,106,011 (GRCm38)	missense	probably damaging	1.00
R6036:Wdfy4	UTSW	14	33,146,990 (GRCm38)	missense	probably damaging	0.97
R6036:Wdfy4	UTSW	14	33,146,990 (GRCm38)	missense	probably damaging	0.97
R6074:Wdfy4	UTSW	14	33,083,639 (GRCm38)	critical splice donor site	probably null
R6135:Wdfy4	UTSW	14	32,971,711 (GRCm38)	missense	probably damaging	0.99
R6276:Wdfy4	UTSW	14	33,109,525 (GRCm38)	missense	possibly damaging	0.54
R6357:Wdfy4	UTSW	14	33,101,049 (GRCm38)	nonsense	probably null
R6370:Wdfy4	UTSW	14	33,068,850 (GRCm38)	missense	probably benign	0.16
R6390:Wdfy4	UTSW	14	33,104,094 (GRCm38)	missense	probably damaging	0.99
R6413:Wdfy4	UTSW	14	32,967,647 (GRCm38)	missense	probably damaging	1.00
R6450:Wdfy4	UTSW	14	33,108,692 (GRCm38)	missense	probably damaging	1.00
R6522:Wdfy4	UTSW	14	33,146,944 (GRCm38)	missense	probably damaging	0.98
R6657:Wdfy4	UTSW	14	33,047,251 (GRCm38)	missense	possibly damaging	0.70
R6761:Wdfy4	UTSW	14	33,095,951 (GRCm38)	missense	possibly damaging	0.89
R6763:Wdfy4	UTSW	14	33,042,512 (GRCm38)	missense	probably damaging	1.00
R6952:Wdfy4	UTSW	14	32,959,966 (GRCm38)	missense	probably damaging	1.00
R6985:Wdfy4	UTSW	14	33,099,117 (GRCm38)	missense	possibly damaging	0.68
R7024:Wdfy4	UTSW	14	32,964,626 (GRCm38)	splice site	probably null
R7101:Wdfy4	UTSW	14	32,960,820 (GRCm38)	missense
R7114:Wdfy4	UTSW	14	32,971,574 (GRCm38)	splice site	probably null
R7139:Wdfy4	UTSW	14	33,151,578 (GRCm38)	missense
R7255:Wdfy4	UTSW	14	32,974,282 (GRCm38)	missense
R7324:Wdfy4	UTSW	14	33,047,314 (GRCm38)	missense
R7379:Wdfy4	UTSW	14	33,151,609 (GRCm38)	missense
R7399:Wdfy4	UTSW	14	33,068,906 (GRCm38)	missense
R7408:Wdfy4	UTSW	14	33,078,307 (GRCm38)	missense
R7410:Wdfy4	UTSW	14	32,974,234 (GRCm38)	missense
R7411:Wdfy4	UTSW	14	33,106,131 (GRCm38)	missense
R7412:Wdfy4	UTSW	14	33,149,584 (GRCm38)	missense
R7445:Wdfy4	UTSW	14	33,070,618 (GRCm38)	nonsense	probably null
R7595:Wdfy4	UTSW	14	32,974,154 (GRCm38)	critical splice donor site	probably null
R7618:Wdfy4	UTSW	14	32,985,739 (GRCm38)	missense
R7622:Wdfy4	UTSW	14	33,078,274 (GRCm38)	missense
R7828:Wdfy4	UTSW	14	32,988,921 (GRCm38)	missense	possibly damaging	0.90
R7888:Wdfy4	UTSW	14	33,090,963 (GRCm38)	missense
R7946:Wdfy4	UTSW	14	33,104,115 (GRCm38)	missense
R7946:Wdfy4	UTSW	14	33,070,748 (GRCm38)	missense
R7986:Wdfy4	UTSW	14	33,104,115 (GRCm38)	missense
R7990:Wdfy4	UTSW	14	33,097,795 (GRCm38)	missense
R8001:Wdfy4	UTSW	14	32,973,535 (GRCm38)	critical splice donor site	probably null
R8010:Wdfy4	UTSW	14	32,971,627 (GRCm38)	missense
R8015:Wdfy4	UTSW	14	33,107,747 (GRCm38)	missense
R8032:Wdfy4	UTSW	14	33,029,086 (GRCm38)	nonsense	probably null
R8041:Wdfy4	UTSW	14	33,154,008 (GRCm38)	critical splice donor site	probably null
R8090:Wdfy4	UTSW	14	33,104,115 (GRCm38)	missense
R8092:Wdfy4	UTSW	14	33,104,115 (GRCm38)	missense
R8112:Wdfy4	UTSW	14	33,104,115 (GRCm38)	missense
R8114:Wdfy4	UTSW	14	33,104,115 (GRCm38)	missense
R8115:Wdfy4	UTSW	14	33,104,115 (GRCm38)	missense
R8117:Wdfy4	UTSW	14	33,104,115 (GRCm38)	missense
R8117:Wdfy4	UTSW	14	32,977,106 (GRCm38)	nonsense	probably null
R8118:Wdfy4	UTSW	14	33,104,115 (GRCm38)	missense
R8140:Wdfy4	UTSW	14	33,142,360 (GRCm38)	missense
R8155:Wdfy4	UTSW	14	33,162,819 (GRCm38)	missense
R8163:Wdfy4	UTSW	14	33,151,588 (GRCm38)	missense
R8293:Wdfy4	UTSW	14	32,974,261 (GRCm38)	missense
R8325:Wdfy4	UTSW	14	32,967,487 (GRCm38)	missense
R8353:Wdfy4	UTSW	14	32,973,624 (GRCm38)	missense	probably benign
R8370:Wdfy4	UTSW	14	33,093,251 (GRCm38)	missense
R8437:Wdfy4	UTSW	14	33,076,375 (GRCm38)	missense
R8497:Wdfy4	UTSW	14	32,966,399 (GRCm38)	missense	probably damaging	0.99
R8545:Wdfy4	UTSW	14	33,078,301 (GRCm38)	missense	probably benign	0.01
R8671:Wdfy4	UTSW	14	32,971,765 (GRCm38)	splice site	probably benign
R8708:Wdfy4	UTSW	14	32,967,532 (GRCm38)	missense
R8747:Wdfy4	UTSW	14	33,152,654 (GRCm38)	missense
R8794:Wdfy4	UTSW	14	33,147,092 (GRCm38)	missense	probably benign	0.03
R8846:Wdfy4	UTSW	14	33,145,148 (GRCm38)	missense
R8880:Wdfy4	UTSW	14	33,073,535 (GRCm38)	nonsense	probably null
R9109:Wdfy4	UTSW	14	33,038,747 (GRCm38)	splice site	probably null
R9131:Wdfy4	UTSW	14	33,097,850 (GRCm38)	missense
R9309:Wdfy4	UTSW	14	33,095,356 (GRCm38)	missense
R9349:Wdfy4	UTSW	14	33,154,039 (GRCm38)	missense
R9451:Wdfy4	UTSW	14	33,133,561 (GRCm38)	missense
R9563:Wdfy4	UTSW	14	32,970,876 (GRCm38)	missense
R9587:Wdfy4	UTSW	14	33,047,273 (GRCm38)	nonsense	probably null
R9599:Wdfy4	UTSW	14	33,133,471 (GRCm38)	missense
R9670:Wdfy4	UTSW	14	33,047,262 (GRCm38)	missense
R9718:Wdfy4	UTSW	14	33,125,936 (GRCm38)	missense
R9742:Wdfy4	UTSW	14	33,088,030 (GRCm38)	missense
X0028:Wdfy4	UTSW	14	33,080,636 (GRCm38)	missense	probably benign
X0053:Wdfy4	UTSW	14	33,162,942 (GRCm38)	start codon destroyed	probably null	0.99
X0062:Wdfy4	UTSW	14	33,107,618 (GRCm38)	splice site	probably null
Z1177:Wdfy4	UTSW	14	33,087,985 (GRCm38)	missense

Predicted Primers

PCR Primer
(F):5'- CTGTGTCCAAGCAAATGAAGGG -3'
(R):5'- CCAGGGATCATGTTGCTGTG -3'

Sequencing Primer
(F):5'- GAGACGTGTGGTGTGCATGTATC -3'
(R):5'- TGGCAGCAGGTATTTCATGG -3'

Posted On

2015-10-08