Mutagenetix > Incidental Mutation

Incidental Mutation 'R4675:Olfr577'

349486

Institutional Source

Beutler Lab

Gene Symbol

Olfr577

Ensembl Gene

ENSMUSG00000043354

Gene Name

olfactory receptor 577

Synonyms

MOR7-2, GA_x6K02T2PBJ9-5685322-5684384

MMRRC Submission

041930-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.133) question?

Stock #

R4675 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

102971180-102975943 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 102973806 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Threonine at position 62 (M62T)

Ref Sequence

ENSEMBL: ENSMUSP00000150712 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000051505] [ENSMUST00000185326] [ENSMUST00000214080] [ENSMUST00000215237]

AlphaFold

Q8VH11

Predicted Effect

probably damaging
Transcript: ENSMUST00000051505
AA Change: M62T

PolyPhen 2 Score 0.992 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000059586
Gene: ENSMUSG00000043354
AA Change: M62T

Domain	Start	End	E-Value	Type
Pfam:7tm_4	34	312	3.3e-140	PFAM
Pfam:7TM_GPCR_Srsx	38	310	1.2e-6	PFAM
Pfam:7tm_1	44	295	7.5e-22	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000185326

SMART Domains

Protein: ENSMUSP00000142459
Gene: ENSMUSG00000073962

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	35	300	9.7e-12	PFAM
Pfam:7tm_1	41	291	1.8e-29	PFAM
Pfam:7tm_4	140	284	2.6e-28	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000214080
AA Change: M62T

PolyPhen 2 Score 0.992 (Sensitivity: 0.70; Specificity: 0.97)

Predicted Effect

probably damaging
Transcript: ENSMUST00000215237
AA Change: M62T

PolyPhen 2 Score 0.992 (Sensitivity: 0.70; Specificity: 0.97)

Meta Mutation Damage Score

0.4324

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.9%

Validation Efficiency

95% (89/94)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 88 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700080E11Rik	T	C	9: 105,144,448	D133G	probably benign	Het
2310061I04Rik	C	A	17: 35,892,928	K291N	probably damaging	Het
Adgra1	A	G	7: 139,876,186	T577A	probably damaging	Het
Akip1	T	A	7: 109,708,981	I152N	possibly damaging	Het
Armc9	A	G	1: 86,202,518	Y8C	probably damaging	Het
Atp1a4	A	T	1: 172,257,656	V66E	possibly damaging	Het
Atp2a3	A	T	11: 72,981,797	T724S	probably damaging	Het
Bmp1	C	A	14: 70,492,844	R416L	probably damaging	Het
Bmt2	G	T	6: 13,663,301	A66E	probably benign	Het
Bscl2	A	T	19: 8,848,159	D403V	possibly damaging	Het
Cbx2	T	A	11: 119,029,109	I500N	probably damaging	Het
Cd84	A	T	1: 171,873,320	H216L	possibly damaging	Het
Ceacam15	T	C	7: 16,673,485	T36A	probably benign	Het
Cebpd	A	G	16: 15,887,521	D66G	probably damaging	Het
Cntnap4	A	G	8: 112,785,836	Y610C	probably damaging	Het
Col11a2	T	C	17: 34,064,293		probably null	Het
Col17a1	T	C	19: 47,663,058		probably null	Het
Cracr2b	A	G	7: 141,463,538	D43G	probably damaging	Het
Dgkz	T	A	2: 91,938,346	K697*	probably null	Het
Dnah7b	T	A	1: 46,217,157	D1873E	possibly damaging	Het
Dst	G	A	1: 34,275,703	E6472K	possibly damaging	Het
Duox2	T	C	2: 122,280,933	D1428G	probably damaging	Het
Elmod2	T	C	8: 83,316,908	N210S	probably damaging	Het
Ephx1	A	G	1: 180,994,691	F220S	probably damaging	Het
F13b	A	G	1: 139,501,804	Y20C	unknown	Het
Fbn2	T	A	18: 58,040,193	N2051I	possibly damaging	Het
Gabrg2	G	A	11: 41,968,823	H201Y	probably damaging	Het
Gbgt1	T	C	2: 28,498,441	F46S	possibly damaging	Het
Gjd4	A	G	18: 9,280,578	S167P	probably damaging	Het
Gm21731	T	C	13: 120,240,826	W53R	probably damaging	Het
Gm281	A	T	14: 13,856,724	D462E	probably benign	Het
Heatr5a	T	C	12: 51,877,347	N2028D	probably benign	Het
Heca	T	C	10: 17,915,309	H333R	probably benign	Het
Herc1	T	C	9: 66,391,458	S625P	probably damaging	Het
Ighv1-55	T	C	12: 115,208,555		probably benign	Het
Ighv5-8	G	A	12: 113,655,157	S64N	probably benign	Het
Itga1	G	T	13: 115,001,691		probably null	Het
Kif21a	C	A	15: 90,940,545	R1342L	possibly damaging	Het
Ksr1	G	A	11: 79,074,360	P118S	possibly damaging	Het
Lat2	T	C	5: 134,606,057	N100S	probably damaging	Het
Lrit3	T	A	3: 129,788,472	D501V	probably damaging	Het
Lrrfip1	T	A	1: 91,103,320		probably null	Het
Lyst	G	A	13: 13,635,383	R546H	probably damaging	Het
Med21	T	A	6: 146,650,193	L114H	probably damaging	Het
Mfap4	A	T	11: 61,485,510		probably benign	Het
Mpdz	G	A	4: 81,383,812	R233W	probably damaging	Het
Mrc2	G	A	11: 105,348,431		probably null	Het
Ncapd3	T	C	9: 27,094,742		probably benign	Het
Neto2	T	G	8: 85,669,704	H104P	probably damaging	Het
Nr1h2	T	C	7: 44,552,555	T36A	possibly damaging	Het
Olfr1252	T	C	2: 89,721,494	I206V	probably benign	Het
Olfr472	A	G	7: 107,903,360	I214M	probably damaging	Het
Olfr497	T	C	7: 108,423,102	V177A	possibly damaging	Het
Olfr53	A	T	7: 140,652,161	M61L	probably damaging	Het
Olfr613	T	G	7: 103,551,976	L64V	probably damaging	Het
Olfr656	T	A	7: 104,618,424	C248*	probably null	Het
Olfr878	T	C	9: 37,919,586	*310R	probably null	Het
Pcbp3	A	G	10: 76,771,035	L241S	possibly damaging	Het
Pcf11	G	A	7: 92,659,777		probably benign	Het
Podxl	G	A	6: 31,526,644	T254M	possibly damaging	Het
Prr27	A	C	5: 87,843,241	E237D	possibly damaging	Het
Rdh16	G	T	10: 127,801,447	V84F	probably damaging	Het
Rnf26	A	T	9: 44,112,131	D273E	probably benign	Het
Rpl13a	A	T	7: 45,126,818		probably benign	Het
Rpl3l	A	G	17: 24,733,610	K239E	probably benign	Het
Rsf1	G	A	7: 97,579,910		probably benign	Het
Rsf1	A	AGGGCGACGG	7: 97,579,904		probably null	Het
Ryk	A	G	9: 102,891,216	D352G	possibly damaging	Het
Setd1b	T	A	5: 123,160,998		probably benign	Het
Sh2b1	G	A	7: 126,471,446	A361V	possibly damaging	Het
Slc35f3	A	T	8: 126,321,196	K92*	probably null	Het
Slc39a10	A	G	1: 46,817,984		probably benign	Het
Slc47a1	G	A	11: 61,363,031	T194I	probably benign	Het
Slc6a3	C	A	13: 73,544,817	N185K	probably damaging	Het
Stag1	T	C	9: 100,848,705	V391A	probably damaging	Het
Syne2	A	C	12: 75,949,301	N2206T	probably damaging	Het
Tbc1d23	C	A	16: 57,182,962	R481I	possibly damaging	Het
Tfam	A	G	10: 71,233,395	S166P	probably benign	Het
Tmem63a	A	G	1: 180,956,491	H212R	probably benign	Het
Txndc11	T	A	16: 11,084,881	Q634L	possibly damaging	Het
Usp31	T	C	7: 121,707,325		probably benign	Het
Vmn1r204	A	T	13: 22,556,792	M198L	probably damaging	Het
Vmn2r17	A	T	5: 109,427,183	T119S	probably benign	Het
Vsig1	A	G	X: 140,933,112	D227G	probably damaging	Het
Zfhx2	G	A	14: 55,067,221	P1102L	probably benign	Het
Zfp715	T	C	7: 43,300,020	Q172R	probably benign	Het
Zfp872	A	G	9: 22,197,405	D53G	probably damaging	Het
Zswim8	G	A	14: 20,714,613	D684N	probably benign	Het

Other mutations in Olfr577

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02246:Olfr577	APN	7	102973744	missense	possibly damaging	0.62
IGL03111:Olfr577	APN	7	102973531	missense	probably damaging	1.00
R1529:Olfr577	UTSW	7	102973879	missense	probably damaging	1.00
R1753:Olfr577	UTSW	7	102973056	missense	probably benign
R3005:Olfr577	UTSW	7	102973258	missense	possibly damaging	0.56
R4457:Olfr577	UTSW	7	102973527	missense	probably damaging	1.00
R4808:Olfr577	UTSW	7	102973911	missense	probably damaging	0.99
R4891:Olfr577	UTSW	7	102973552	missense	probably benign	0.12
R4917:Olfr577	UTSW	7	102973407	missense	possibly damaging	0.93
R4918:Olfr577	UTSW	7	102973407	missense	possibly damaging	0.93
R5328:Olfr577	UTSW	7	102973968	missense	possibly damaging	0.46
R6375:Olfr577	UTSW	7	102973753	missense	probably damaging	1.00
R6683:Olfr577	UTSW	7	102973713	missense	probably benign	0.05
R6958:Olfr577	UTSW	7	102973884	missense	possibly damaging	0.67
R7022:Olfr577	UTSW	7	102973968	missense	possibly damaging	0.46
R7429:Olfr577	UTSW	7	102973762	missense	probably damaging	1.00
R7430:Olfr577	UTSW	7	102973762	missense	probably damaging	1.00
R7490:Olfr577	UTSW	7	102973810	missense	probably damaging	1.00
R7808:Olfr577	UTSW	7	102973110	missense	possibly damaging	0.56
R8169:Olfr577	UTSW	7	102973338	missense	probably damaging	0.99
R8544:Olfr577	UTSW	7	102973731	missense	probably damaging	1.00
R9027:Olfr577	UTSW	7	102973353	missense	probably damaging	1.00
R9265:Olfr577	UTSW	7	102973905	nonsense	probably null
X0027:Olfr577	UTSW	7	102973686	missense	probably benign	0.05
Z1176:Olfr577	UTSW	7	102973309	missense	not run
Z1177:Olfr577	UTSW	7	102973309	missense	not run

Predicted Primers

PCR Primer
(F):5'- ACAATCACTGTGTGGGTGAG -3'
(R):5'- CCCTAGGAAATGGAAGCATGTC -3'

Sequencing Primer
(F):5'- TGCAAAGGACGACAGATAGCC -3'
(R):5'- GAAGCATGTCTTCTACCTTCTTG -3'

Posted On

2015-10-08