Mutagenetix > Incidental Mutation

Incidental Mutation 'R4676:Naa16'

349604

Institutional Source

Beutler Lab

Gene Symbol

Naa16

Ensembl Gene

ENSMUSG00000022020

Gene Name

N(alpha)-acetyltransferase 16, NatA auxiliary subunit

Synonyms

Narg1l, 1300019C06Rik

MMRRC Submission

042013-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.099) question?

Stock #

R4676 (G1)

Quality Score

197

Status

Validated

Chromosome

Chromosomal Location

79325269-79390778 bp(-) (GRCm38)

Type of Mutation

unclassified

DNA Base Change (assembly)

A to G at 79336348 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000131268 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000022597] [ENSMUST00000163486]

AlphaFold

Q9DBB4

Predicted Effect

probably benign
Transcript: ENSMUST00000022597

SMART Domains

Protein: ENSMUSP00000022597
Gene: ENSMUSG00000022020

Domain	Start	End	E-Value	Type
TPR	46	79	2.99e1	SMART
TPR	80	113	2.98e-3	SMART
Blast:TPR	224	257	1e-10	BLAST
TPR	374	407	9.96e0	SMART
TPR	408	441	7.47e0	SMART
low complexity region	616	633	N/A	INTRINSIC
Blast:TPR	672	705	3e-12	BLAST
low complexity region	830	841	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000163486

SMART Domains

Protein: ENSMUSP00000131268
Gene: ENSMUSG00000022020

Domain	Start	End	E-Value	Type
TPR	12	45	2.99e1	SMART
TPR	46	79	2.98e-3	SMART
Blast:TPR	190	223	3e-10	BLAST
TPR	340	373	9.96e0	SMART
TPR	374	407	7.47e0	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000171928

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000227775

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.8%

Validation Efficiency

98% (93/95)

Allele List at MGI

Other mutations in this stock

Total: 84 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3110009E18Rik	T	A	1: 120,150,652 (GRCm38)	I13N	probably damaging	Het
Abcd3	T	A	3: 121,774,166 (GRCm38)	T409S	possibly damaging	Het
Acad12	C	A	5: 121,607,171 (GRCm38)	W317L	probably damaging	Het
Acap1	T	A	11: 69,889,468 (GRCm38)	M50L	probably benign	Het
Acvr1b	T	A	15: 101,202,986 (GRCm38)	V343E	probably damaging	Het
Adgb	C	A	10: 10,426,710 (GRCm38)	G371W	probably damaging	Het
Akap9	A	G	5: 4,032,774 (GRCm38)	K1966R	probably damaging	Het
Akap9	C	T	5: 4,064,515 (GRCm38)	Q48*	probably null	Het
Ano10	T	C	9: 122,263,787 (GRCm38)	R159G	probably damaging	Het
Anxa7	T	C	14: 20,467,915 (GRCm38)	M128V	probably benign	Het
Arhgef40	C	A	14: 51,990,959 (GRCm38)	C554*	probably null	Het
Atf6	T	C	1: 170,787,410 (GRCm38)	Y538C	probably damaging	Het
Atp8b1	A	C	18: 64,538,678 (GRCm38)	D1091E	probably benign	Het
BC034090	A	G	1: 155,226,264 (GRCm38)	Y85H	possibly damaging	Het
Bcas2	A	G	3: 103,175,701 (GRCm38)		probably benign	Het
Bnc2	T	C	4: 84,292,819 (GRCm38)	N463D	probably damaging	Het
Capn7	A	T	14: 31,359,259 (GRCm38)	H411L	possibly damaging	Het
Cavin3	T	C	7: 105,481,113 (GRCm38)	E164G	probably damaging	Het
Ccdc191	T	C	16: 43,939,173 (GRCm38)		probably benign	Het
Ccdc88b	A	G	19: 6,853,000 (GRCm38)	V858A	probably benign	Het
Clcn3	A	G	8: 60,930,651 (GRCm38)		probably benign	Het
Commd6	T	C	14: 101,640,284 (GRCm38)		probably benign	Het
Cspg4b	T	G	13: 113,368,808 (GRCm38)	L2157R	probably damaging	Het
Cspg4b	C	A	13: 113,368,807 (GRCm38)	L2157I	probably damaging	Het
Cul3	G	A	1: 80,271,674 (GRCm38)	L561F	probably damaging	Het
Cyfip1	T	A	7: 55,875,013 (GRCm38)	I131N	probably damaging	Het
Dlgap3	T	A	4: 127,233,761 (GRCm38)	Y741N	probably damaging	Het
Dnaaf10	T	C	11: 17,229,794 (GRCm38)	V265A	probably benign	Het
Dnah5	A	T	15: 28,295,260 (GRCm38)	I1380F	possibly damaging	Het
Dync1h1	G	T	12: 110,662,541 (GRCm38)	L4177F	probably damaging	Het
Ethe1	G	A	7: 24,607,894 (GRCm38)	V178M	probably damaging	Het
Flnc	A	T	6: 29,445,154 (GRCm38)		probably null	Het
Get3	C	T	8: 85,018,873 (GRCm38)	A219T	probably benign	Het
Glt8d1	T	C	14: 31,006,692 (GRCm38)	F26L	probably benign	Het
Gm5493	T	A	17: 22,748,081 (GRCm38)	D63E	probably benign	Het
Gm9996	T	A	10: 29,143,838 (GRCm38)		probably benign	Het
Gnl2	T	G	4: 125,053,473 (GRCm38)	S629R	possibly damaging	Het
Gpbp1l1	T	C	4: 116,590,265 (GRCm38)	S381P	probably damaging	Het
Igsf10	A	C	3: 59,325,949 (GRCm38)	F1788V	probably benign	Het
Inpp5d	C	A	1: 87,715,142 (GRCm38)	P935Q	probably damaging	Het
Itch	A	C	2: 155,199,435 (GRCm38)	I468L	probably benign	Het
Itga5	A	T	15: 103,357,210 (GRCm38)	Y192N	probably damaging	Het
Itih3	T	A	14: 30,918,949 (GRCm38)	Q302L	probably null	Het
Itih3	T	A	14: 30,921,686 (GRCm38)	Q121L	possibly damaging	Het
Kctd17	T	A	15: 78,435,759 (GRCm38)		probably benign	Het
Lsm1	T	C	8: 25,793,689 (GRCm38)	L43P	probably damaging	Het
Magi3	A	T	3: 104,015,825 (GRCm38)	M1192K	probably benign	Het
Mecom	A	G	3: 30,268,668 (GRCm38)		probably benign	Het
Minar1	A	G	9: 89,601,553 (GRCm38)	V597A	probably damaging	Het
Mtmr3	A	T	11: 4,527,855 (GRCm38)	F63Y	probably benign	Het
Neto1	A	T	18: 86,398,302 (GRCm38)	T45S	possibly damaging	Het
Nlrp4a	A	T	7: 26,450,229 (GRCm38)	R420S	probably damaging	Het
Nr1h4	T	C	10: 89,473,874 (GRCm38)	D317G	probably damaging	Het
Or8b53	A	G	9: 38,755,659 (GRCm38)		probably benign	Het
Or9i16	C	T	19: 13,887,401 (GRCm38)	D270N	probably damaging	Het
Or9q2	T	A	19: 13,795,474 (GRCm38)	I46F	possibly damaging	Het
Pde5a	A	T	3: 122,747,893 (GRCm38)	M11L	possibly damaging	Het
Plxnb1	G	A	9: 109,110,435 (GRCm38)	R1416Q	possibly damaging	Het
Polm	A	T	11: 5,835,749 (GRCm38)	Y141*	probably null	Het
Rxfp1	A	G	3: 79,705,668 (GRCm38)	F32L	probably damaging	Het
Scrt1	T	A	15: 76,521,668 (GRCm38)	D13V	possibly damaging	Het
Slc1a7	T	A	4: 107,977,674 (GRCm38)	V79E	possibly damaging	Het
Snurf	T	C	7: 59,995,522 (GRCm38)	Q48R	probably benign	Het
Srek1	T	C	13: 103,758,187 (GRCm38)		probably benign	Het
Stxbp5l	T	C	16: 37,255,884 (GRCm38)	S267G	probably damaging	Het
Taf5	C	T	19: 47,074,970 (GRCm38)	R320W	probably damaging	Het
Tars2	A	T	3: 95,753,091 (GRCm38)	N106K	probably damaging	Het
Tatdn3	A	T	1: 191,049,334 (GRCm38)	L207Q	probably damaging	Het
Tdrd6	T	C	17: 43,627,610 (GRCm38)	E849G	probably damaging	Het
Tedc2	T	C	17: 24,220,011 (GRCm38)	T111A	probably benign	Het
Tfg	T	A	16: 56,694,491 (GRCm38)		probably null	Het
Tgds	A	T	14: 118,116,231 (GRCm38)	S225T	probably benign	Het
Tnks2	T	A	19: 36,875,271 (GRCm38)	Y134*	probably null	Het
Trappc1	T	A	11: 69,325,530 (GRCm38)	V134D	probably damaging	Het
Ttc3	C	A	16: 94,442,761 (GRCm38)	P853Q	probably damaging	Het
Ttc7	C	A	17: 87,370,735 (GRCm38)		probably benign	Het
Ttf1	A	G	2: 29,074,594 (GRCm38)	S643G	probably damaging	Het
Tubgcp3	A	T	8: 12,650,171 (GRCm38)	S338T	probably damaging	Het
Ugt1a6a	T	C	1: 88,139,285 (GRCm38)	I271T	possibly damaging	Het
Vipas39	T	A	12: 87,241,301 (GRCm38)	Y445F	probably damaging	Het
Vmn1r31	A	C	6: 58,472,013 (GRCm38)	I289S	probably damaging	Het
Zfp112	A	G	7: 24,126,260 (GRCm38)	E551G	probably damaging	Het
Zfp397	T	A	18: 23,960,797 (GRCm38)	Y446*	probably null	Het
Zfp442	A	T	2: 150,409,606 (GRCm38)	H124Q	probably damaging	Het

Other mutations in Naa16

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00551:Naa16	APN	14	79,355,729 (GRCm38)	missense	probably damaging	1.00
IGL01025:Naa16	APN	14	79,384,756 (GRCm38)	missense	probably damaging	1.00
IGL01155:Naa16	APN	14	79,384,715 (GRCm38)	missense	probably damaging	0.98
IGL01335:Naa16	APN	14	79,345,116 (GRCm38)	splice site	probably benign
IGL01981:Naa16	APN	14	79,381,516 (GRCm38)	missense	probably benign	0.05
IGL02230:Naa16	APN	14	79,377,361 (GRCm38)	splice site	probably benign
IGL02313:Naa16	APN	14	79,384,668 (GRCm38)	missense	probably damaging	1.00
IGL02418:Naa16	APN	14	79,383,366 (GRCm38)	missense	probably damaging	1.00
IGL02544:Naa16	APN	14	79,335,820 (GRCm38)	missense	probably damaging	1.00
IGL03051:Naa16	APN	14	79,369,082 (GRCm38)	missense	probably benign	0.01
IGL03064:Naa16	APN	14	79,339,628 (GRCm38)	missense	probably damaging	0.98
IGL03205:Naa16	APN	14	79,356,512 (GRCm38)	missense	possibly damaging	0.89
PIT4508001:Naa16	UTSW	14	79,369,087 (GRCm38)	missense	probably benign	0.15
R0651:Naa16	UTSW	14	79,351,392 (GRCm38)	missense	probably damaging	1.00
R1429:Naa16	UTSW	14	79,359,527 (GRCm38)	missense	probably benign	0.01
R1674:Naa16	UTSW	14	79,387,057 (GRCm38)	start codon destroyed	probably null	0.65
R1693:Naa16	UTSW	14	79,351,456 (GRCm38)	missense	probably damaging	1.00
R1874:Naa16	UTSW	14	79,355,743 (GRCm38)	missense	possibly damaging	0.62
R1992:Naa16	UTSW	14	79,356,491 (GRCm38)	missense	probably damaging	1.00
R2015:Naa16	UTSW	14	79,345,059 (GRCm38)	missense	probably damaging	1.00
R2391:Naa16	UTSW	14	79,370,049 (GRCm38)	missense	probably benign	0.16
R2847:Naa16	UTSW	14	79,335,883 (GRCm38)	missense	probably damaging	1.00
R2848:Naa16	UTSW	14	79,335,883 (GRCm38)	missense	probably damaging	1.00
R2877:Naa16	UTSW	14	79,343,298 (GRCm38)	missense	probably benign	0.00
R3884:Naa16	UTSW	14	79,343,262 (GRCm38)	missense	probably damaging	0.98
R4001:Naa16	UTSW	14	79,343,121 (GRCm38)	splice site	probably null
R4199:Naa16	UTSW	14	79,355,871 (GRCm38)	missense	probably damaging	1.00
R4638:Naa16	UTSW	14	79,340,033 (GRCm38)	splice site	probably null
R4690:Naa16	UTSW	14	79,345,057 (GRCm38)	missense	probably damaging	1.00
R4952:Naa16	UTSW	14	79,345,085 (GRCm38)	missense	probably damaging	1.00
R5087:Naa16	UTSW	14	79,377,415 (GRCm38)	missense	possibly damaging	0.68
R5104:Naa16	UTSW	14	79,384,700 (GRCm38)	nonsense	probably null
R5729:Naa16	UTSW	14	79,355,780 (GRCm38)	missense	probably damaging	1.00
R6178:Naa16	UTSW	14	79,383,340 (GRCm38)	missense	possibly damaging	0.93
R6960:Naa16	UTSW	14	79,359,471 (GRCm38)	missense	possibly damaging	0.65
R7794:Naa16	UTSW	14	79,377,494 (GRCm38)	missense	probably damaging	1.00
R7936:Naa16	UTSW	14	79,341,046 (GRCm38)	missense	possibly damaging	0.47
R8356:Naa16	UTSW	14	79,359,475 (GRCm38)	missense	probably benign	0.00
R8456:Naa16	UTSW	14	79,359,475 (GRCm38)	missense	probably benign	0.00
R8892:Naa16	UTSW	14	79,390,576 (GRCm38)	missense	probably benign	0.32
R8931:Naa16	UTSW	14	79,344,955 (GRCm38)	missense	probably damaging	1.00
R9010:Naa16	UTSW	14	79,370,042 (GRCm38)	missense	probably benign	0.01
R9068:Naa16	UTSW	14	79,374,849 (GRCm38)	missense	probably benign	0.18
R9360:Naa16	UTSW	14	79,356,503 (GRCm38)	missense	probably benign	0.05
R9688:Naa16	UTSW	14	79,335,869 (GRCm38)	nonsense	probably null
X0064:Naa16	UTSW	14	79,351,389 (GRCm38)	missense	probably damaging	1.00
Z1177:Naa16	UTSW	14	79,344,979 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCTCCGGATGAGCTAGGAAG -3'
(R):5'- GTGACTAAACACTGTAGCCACATG -3'

Sequencing Primer
(F):5'- AGCTAGGAAGAGGTGTTTACTTC -3'
(R):5'- GTAAGCCTTAGGAGTTACTCATCC -3'

Posted On

2015-10-08