Mutagenetix > Incidental Mutation

Incidental Mutation 'R4678:Zeb2'

349722

Institutional Source

Beutler Lab

Gene Symbol

Zeb2

Ensembl Gene

ENSMUSG00000026872

Gene Name

zinc finger E-box binding homeobox 2

Synonyms

SIP1, D130016B08Rik, 9130203F04Rik, Zfhx1b, Zfx1b

MMRRC Submission

041931-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4678 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

44873644-45007407 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 44886353 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glutamic Acid at position 857 (D857E)

Ref Sequence

ENSEMBL: ENSMUSP00000134747 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000028229] [ENSMUST00000068415] [ENSMUST00000076836] [ENSMUST00000176438] [ENSMUST00000176732] [ENSMUST00000177302] [ENSMUST00000200844] [ENSMUST00000201804]

AlphaFold

Q9R0G7

Predicted Effect

probably benign
Transcript: ENSMUST00000028229
AA Change: D901E

PolyPhen 2 Score 0.012 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000028229
Gene: ENSMUSG00000026872
AA Change: D901E

Domain	Start	End	E-Value	Type
low complexity region	78	90	N/A	INTRINSIC
ZnF_C2H2	211	234	2.09e-3	SMART
ZnF_C2H2	241	263	9.88e-5	SMART
ZnF_C2H2	282	304	4.87e-4	SMART
ZnF_C2H2	310	330	1.86e1	SMART
low complexity region	352	364	N/A	INTRINSIC
ZnF_C2H2	581	601	5.54e1	SMART
HOX	644	706	2.05e-3	SMART
low complexity region	778	808	N/A	INTRINSIC
low complexity region	841	856	N/A	INTRINSIC
low complexity region	870	881	N/A	INTRINSIC
ZnF_C2H2	999	1021	4.47e-3	SMART
ZnF_C2H2	1027	1049	2.17e-1	SMART
ZnF_C2H2	1055	1076	1.89e-1	SMART
low complexity region	1083	1097	N/A	INTRINSIC
low complexity region	1134	1150	N/A	INTRINSIC
low complexity region	1158	1168	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000068415
AA Change: D857E

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000069685
Gene: ENSMUSG00000026872
AA Change: D857E

Domain	Start	End	E-Value	Type
low complexity region	78	90	N/A	INTRINSIC
ZnF_C2H2	211	234	2.09e-3	SMART
ZnF_C2H2	241	263	9.88e-5	SMART
ZnF_C2H2	282	304	4.87e-4	SMART
ZnF_C2H2	310	330	1.86e1	SMART
low complexity region	352	364	N/A	INTRINSIC
ZnF_C2H2	581	601	5.54e1	SMART
HOX	644	706	2.05e-3	SMART
low complexity region	778	808	N/A	INTRINSIC
low complexity region	841	856	N/A	INTRINSIC
low complexity region	870	881	N/A	INTRINSIC
ZnF_C2H2	999	1021	4.47e-3	SMART
ZnF_C2H2	1027	1049	2.17e-1	SMART
ZnF_C2H2	1055	1076	1.89e-1	SMART
low complexity region	1083	1097	N/A	INTRINSIC
low complexity region	1134	1150	N/A	INTRINSIC
low complexity region	1158	1168	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000076836
AA Change: D856E

PolyPhen 2 Score 0.012 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000076111
Gene: ENSMUSG00000026872
AA Change: D856E

Domain	Start	End	E-Value	Type
low complexity region	78	90	N/A	INTRINSIC
ZnF_C2H2	210	233	2.09e-3	SMART
ZnF_C2H2	240	262	9.88e-5	SMART
ZnF_C2H2	281	303	4.87e-4	SMART
ZnF_C2H2	309	329	1.86e1	SMART
low complexity region	351	363	N/A	INTRINSIC
ZnF_C2H2	580	600	5.54e1	SMART
HOX	643	705	2.05e-3	SMART
low complexity region	777	807	N/A	INTRINSIC
low complexity region	840	855	N/A	INTRINSIC
low complexity region	869	880	N/A	INTRINSIC
ZnF_C2H2	998	1020	4.47e-3	SMART
ZnF_C2H2	1026	1048	2.17e-1	SMART
ZnF_C2H2	1054	1075	1.89e-1	SMART
low complexity region	1082	1096	N/A	INTRINSIC
low complexity region	1133	1149	N/A	INTRINSIC
low complexity region	1157	1167	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000176438
AA Change: D857E

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000134849
Gene: ENSMUSG00000026872
AA Change: D857E

Domain	Start	End	E-Value	Type
low complexity region	78	90	N/A	INTRINSIC
ZnF_C2H2	211	234	2.09e-3	SMART
ZnF_C2H2	241	263	9.88e-5	SMART
ZnF_C2H2	282	304	4.87e-4	SMART
ZnF_C2H2	310	330	1.86e1	SMART
low complexity region	352	364	N/A	INTRINSIC
ZnF_C2H2	581	601	5.54e1	SMART
HOX	644	706	2.05e-3	SMART
low complexity region	778	808	N/A	INTRINSIC
low complexity region	841	856	N/A	INTRINSIC
low complexity region	870	881	N/A	INTRINSIC
ZnF_C2H2	999	1021	4.47e-3	SMART
ZnF_C2H2	1027	1049	2.17e-1	SMART
ZnF_C2H2	1055	1076	1.89e-1	SMART
low complexity region	1083	1097	N/A	INTRINSIC
low complexity region	1134	1150	N/A	INTRINSIC
low complexity region	1158	1168	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000176732

SMART Domains

Protein: ENSMUSP00000135393
Gene: ENSMUSG00000026872

Domain	Start	End	E-Value	Type
low complexity region	14	26	N/A	INTRINSIC
ZnF_C2H2	60	83	2.09e-3	SMART
ZnF_C2H2	90	112	9.88e-5	SMART
ZnF_C2H2	131	153	4.87e-4	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000177302
AA Change: D857E

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000134747
Gene: ENSMUSG00000026872
AA Change: D857E

Domain	Start	End	E-Value	Type
low complexity region	78	90	N/A	INTRINSIC
ZnF_C2H2	211	234	2.09e-3	SMART
ZnF_C2H2	241	263	9.88e-5	SMART
ZnF_C2H2	282	304	4.87e-4	SMART
ZnF_C2H2	310	330	1.86e1	SMART
low complexity region	352	364	N/A	INTRINSIC
ZnF_C2H2	581	601	5.54e1	SMART
HOX	644	706	2.05e-3	SMART
low complexity region	778	808	N/A	INTRINSIC
low complexity region	841	856	N/A	INTRINSIC
low complexity region	870	881	N/A	INTRINSIC
ZnF_C2H2	999	1021	4.47e-3	SMART
ZnF_C2H2	1027	1049	2.17e-1	SMART
ZnF_C2H2	1055	1076	1.89e-1	SMART
low complexity region	1083	1097	N/A	INTRINSIC
low complexity region	1134	1150	N/A	INTRINSIC
low complexity region	1158	1168	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000200844
AA Change: D833E

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000144421
Gene: ENSMUSG00000026872
AA Change: D833E

Domain	Start	End	E-Value	Type
low complexity region	78	90	N/A	INTRINSIC
ZnF_C2H2	187	210	9.2e-6	SMART
ZnF_C2H2	217	239	4.2e-7	SMART
ZnF_C2H2	258	280	2e-6	SMART
ZnF_C2H2	286	306	8e-2	SMART
low complexity region	328	340	N/A	INTRINSIC
ZnF_C2H2	557	577	2.4e-1	SMART
HOX	620	682	1.1e-5	SMART
low complexity region	754	784	N/A	INTRINSIC
low complexity region	817	832	N/A	INTRINSIC
low complexity region	846	857	N/A	INTRINSIC
ZnF_C2H2	975	997	1.9e-5	SMART
ZnF_C2H2	1003	1025	9.6e-4	SMART
ZnF_C2H2	1031	1052	7.9e-4	SMART
low complexity region	1059	1073	N/A	INTRINSIC
low complexity region	1110	1126	N/A	INTRINSIC
low complexity region	1134	1144	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000201804

SMART Domains

Protein: ENSMUSP00000144637
Gene: ENSMUSG00000026872

Domain	Start	End	E-Value	Type
low complexity region	107	119	N/A	INTRINSIC
ZnF_C2H2	240	263	9.2e-6	SMART
ZnF_C2H2	270	292	4.2e-7	SMART
ZnF_C2H2	311	333	2e-6	SMART
ZnF_C2H2	339	359	8e-2	SMART
low complexity region	381	393	N/A	INTRINSIC
ZnF_C2H2	610	630	2.4e-1	SMART
HOX	673	731	1.2e-3	SMART

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 96.9%
20x: 94.1%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the Zfh1 family of 2-handed zinc finger/homeodomain proteins. It is located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Mutations in this gene are associated with Hirschsprung disease/Mowat-Wilson syndrome. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jan 2010]
PHENOTYPE: Homozygous null mutants exhibit a variety of defects at embryonic day 8.5 and die between embryonic days 9.5 and 10.5. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 105 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110025L11Rik	T	C	16: 88,860,873 (GRCm39)		probably benign	Het
Abcc4	A	G	14: 118,865,103 (GRCm39)	S308P	probably damaging	Het
Agr3	G	T	12: 35,997,832 (GRCm39)	V115L	probably damaging	Het
Ahr	A	T	12: 35,557,463 (GRCm39)	I319N	probably damaging	Het
Alpk1	A	T	3: 127,473,507 (GRCm39)	V832D	probably damaging	Het
Ano1	T	C	7: 144,223,289 (GRCm39)	T78A	probably benign	Het
Apob	G	A	12: 8,045,585 (GRCm39)	G897D	probably damaging	Het
Arfgef1	A	G	1: 10,212,891 (GRCm39)	F1677L	probably benign	Het
Arhgap40	G	T	2: 158,374,226 (GRCm39)	G217W	probably benign	Het
Arhgef4	A	G	1: 34,761,749 (GRCm39)	E335G	unknown	Het
Calcoco2	T	C	11: 95,994,374 (GRCm39)	T60A	probably damaging	Het
Catsperg1	A	T	7: 28,889,721 (GRCm39)	S741T	probably benign	Het
Ccdc181	T	G	1: 164,105,846 (GRCm39)	I27S	probably damaging	Het
Ceacam9	A	T	7: 16,459,334 (GRCm39)	Y211F	probably damaging	Het
Cep41	T	C	6: 30,671,318 (GRCm39)		probably null	Het
Cercam	T	A	2: 29,759,689 (GRCm39)	L45Q	probably damaging	Het
Cnnm2	G	A	19: 46,751,685 (GRCm39)	V492M	possibly damaging	Het
Cntn3	A	G	6: 102,180,981 (GRCm39)	V738A	probably damaging	Het
Coq8a	T	C	1: 179,997,646 (GRCm39)	E351G	probably damaging	Het
Cyp3a57	T	C	5: 145,307,538 (GRCm39)		probably null	Het
Dbn1	T	C	13: 55,623,071 (GRCm39)	I471V	probably benign	Het
Ddx21	G	T	10: 62,429,782 (GRCm39)	Q321K	probably benign	Het
Dhx8	T	C	11: 101,630,634 (GRCm39)	V347A	probably damaging	Het
Dkc1	A	G	X: 74,144,598 (GRCm39)	I215V	probably benign	Homo
Dlg2	A	G	7: 92,077,788 (GRCm39)	I685V	possibly damaging	Het
Dusp11	A	C	6: 85,930,363 (GRCm39)	N140K	probably damaging	Het
Ece2	A	G	16: 20,459,468 (GRCm39)	K454R	probably damaging	Het
Eno4	T	A	19: 58,935,181 (GRCm39)	V131E	probably damaging	Het
Enpep	A	G	3: 129,097,362 (GRCm39)		probably null	Het
Etv1	T	C	12: 38,885,219 (GRCm39)	Y236H	probably damaging	Het
F2rl2	A	G	13: 95,837,140 (GRCm39)	T62A	probably benign	Het
Fbxl21	T	C	13: 56,684,862 (GRCm39)	V296A	probably damaging	Het
Fig4	T	C	10: 41,148,994 (GRCm39)	I153V	probably benign	Het
Fis1	T	A	5: 136,991,951 (GRCm39)	N41K	possibly damaging	Het
Fras1	T	A	5: 96,848,427 (GRCm39)	M1814K	probably benign	Het
Frem2	T	A	3: 53,451,792 (GRCm39)	I2266F	probably benign	Het
Gm10330	A	T	12: 23,829,843 (GRCm39)	*113R	probably null	Het
Gm12185	T	A	11: 48,806,367 (GRCm39)	I275F	probably benign	Het
Gsdme	A	T	6: 50,206,304 (GRCm39)	C180S	possibly damaging	Het
Herc1	A	G	9: 66,323,551 (GRCm39)	E1355G	probably benign	Het
Hnrnpm	A	T	17: 33,869,185 (GRCm39)	I453N	possibly damaging	Het
Hspb9	T	C	11: 100,604,896 (GRCm39)	L74P	probably damaging	Het
Ift46	C	A	9: 44,695,260 (GRCm39)	Y85*	probably null	Het
Insyn2b	T	G	11: 34,353,227 (GRCm39)	L423R	probably damaging	Het
Ints3	T	C	3: 90,315,817 (GRCm39)	T316A	possibly damaging	Het
Isg20	C	T	7: 78,564,076 (GRCm39)		probably benign	Het
Itga11	T	A	9: 62,642,639 (GRCm39)	N187K	probably damaging	Het
Klhl9	G	A	4: 88,639,161 (GRCm39)	T360I	probably damaging	Het
Krt39	T	A	11: 99,411,826 (GRCm39)	I87F	probably benign	Het
Krtap19-4	G	A	16: 88,681,734 (GRCm39)	S74F	unknown	Het
Lgi1	G	A	19: 38,289,737 (GRCm39)	V268I	probably damaging	Het
Lrrc46	G	A	11: 96,925,719 (GRCm39)	P248S	probably benign	Het
Lrrn4	C	T	2: 132,721,488 (GRCm39)	V110I	probably benign	Het
Mamstr	C	A	7: 45,294,116 (GRCm39)		probably benign	Het
Micu3	T	A	8: 40,833,718 (GRCm39)	F451I	probably damaging	Het
Mid1	C	A	X: 168,768,044 (GRCm39)	D130E	possibly damaging	Het
Mkks	T	C	2: 136,722,201 (GRCm39)	T319A	probably benign	Het
Mob3c	A	G	4: 115,690,968 (GRCm39)		probably null	Het
Muc6	T	C	7: 141,230,554 (GRCm39)	E1192G	probably benign	Het
Ndc80	A	G	17: 71,827,753 (GRCm39)		probably null	Het
Nfx1	A	G	4: 41,012,070 (GRCm39)	K807E	probably benign	Het
Nrxn1	A	T	17: 90,930,850 (GRCm39)	L181Q	probably damaging	Het
Nsun7	A	G	5: 66,418,407 (GRCm39)	S46G	probably benign	Het
Nup98	A	T	7: 101,834,038 (GRCm39)	L308H	probably damaging	Het
Or4c115	C	A	2: 88,927,860 (GRCm39)	W137L	probably benign	Het
Or52j3	T	C	7: 102,836,098 (GRCm39)	F97L	probably damaging	Het
Or6f2	T	C	7: 139,756,141 (GRCm39)	V36A	probably benign	Het
Or8k32	T	C	2: 86,369,077 (GRCm39)	M61V	possibly damaging	Het
Palb2	T	C	7: 121,726,589 (GRCm39)	K427R	probably damaging	Het
Pcdha1	C	A	18: 37,063,965 (GRCm39)	Q210K	probably benign	Het
Pcdhb2	T	C	18: 37,429,260 (GRCm39)	L411P	probably damaging	Het
Pde4dip	C	A	3: 97,602,321 (GRCm39)	D2252Y	probably damaging	Het
Plekhg4	T	A	8: 106,107,003 (GRCm39)	Y899*	probably null	Het
Plxnb2	G	T	15: 89,045,131 (GRCm39)	T1105K	probably benign	Het
Psmd2	T	C	16: 20,478,719 (GRCm39)	V606A	probably damaging	Het
Ptprq	A	T	10: 107,521,043 (GRCm39)	F710I	probably benign	Het
Rad51ap2	A	G	12: 11,506,552 (GRCm39)	E158G	probably damaging	Het
Rasip1	T	A	7: 45,277,247 (GRCm39)	H18Q	possibly damaging	Het
Rassf8	A	G	6: 145,760,808 (GRCm39)	K45E	probably damaging	Het
Rrn3	T	A	16: 13,613,940 (GRCm39)	M284K	probably damaging	Het
Rsf1	GGCG	GGCGACGGCTGCG	7: 97,229,113 (GRCm39)		probably benign	Het
Rtn2	C	A	7: 19,027,820 (GRCm39)	N403K	probably damaging	Het
Rusc1	A	G	3: 88,997,027 (GRCm39)	W462R	probably damaging	Het
Sdc3	T	A	4: 130,545,907 (GRCm39)		probably benign	Het
Secisbp2l	C	T	2: 125,582,657 (GRCm39)	G933D	possibly damaging	Het
Slc12a2	T	A	18: 58,039,032 (GRCm39)	C537*	probably null	Het
Slc4a2	G	A	5: 24,639,238 (GRCm39)		probably null	Het
Spsb4	T	A	9: 96,877,744 (GRCm39)	D193V	probably damaging	Het
Strn	A	C	17: 78,984,780 (GRCm39)	F288V	probably damaging	Het
Stx18	G	A	5: 38,293,712 (GRCm39)		probably benign	Het
Tfcp2l1	T	A	1: 118,596,378 (GRCm39)	M371K	probably benign	Het
Thnsl1	A	G	2: 21,216,352 (GRCm39)		probably null	Het
Tlr11	C	T	14: 50,598,439 (GRCm39)	H142Y	possibly damaging	Het
Tor1aip2	T	A	1: 155,940,780 (GRCm39)	I362K	probably damaging	Het
Trmt13	T	C	3: 116,383,404 (GRCm39)	K125E	probably damaging	Het
Trpm8	T	C	1: 88,264,851 (GRCm39)	V320A	probably benign	Het
Ubr3	A	T	2: 69,766,263 (GRCm39)	H377L	probably damaging	Het
Usp8	A	G	2: 126,567,349 (GRCm39)	R123G	probably null	Het
Vim	T	A	2: 13,579,775 (GRCm39)	L178Q	probably damaging	Het
Vmn1r231	A	C	17: 21,110,489 (GRCm39)	V142G	possibly damaging	Het
Vmn1r83	A	G	7: 12,055,697 (GRCm39)	M120T	possibly damaging	Het
Vwce	G	T	19: 10,642,012 (GRCm39)	V913F	possibly damaging	Het
Zbtb18	C	A	1: 177,275,285 (GRCm39)	T215K	probably benign	Het
Zfp106	G	A	2: 120,364,221 (GRCm39)	H729Y	probably damaging	Het
Zfp977	C	A	7: 42,229,437 (GRCm39)	A363S	probably benign	Het

Other mutations in Zeb2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00900:Zeb2	APN	2	44,887,287 (GRCm39)	missense	probably damaging	1.00
IGL01639:Zeb2	APN	2	44,887,269 (GRCm39)	missense	probably benign
IGL02016:Zeb2	APN	2	44,878,886 (GRCm39)	missense	possibly damaging	0.71
IGL02337:Zeb2	APN	2	44,887,242 (GRCm39)	missense	probably damaging	0.96
IGL02745:Zeb2	APN	2	44,884,487 (GRCm39)	unclassified	probably benign
IGL02893:Zeb2	APN	2	44,886,619 (GRCm39)	missense	probably benign	0.03
IGL03412:Zeb2	APN	2	44,892,720 (GRCm39)	intron	probably benign
Blight	UTSW	2	45,000,040 (GRCm39)	missense	possibly damaging	0.95
Dropped	UTSW	2	45,000,053 (GRCm39)	missense	possibly damaging	0.66
Okapi	UTSW	2	44,887,168 (GRCm39)	missense	probably damaging	1.00
sable	UTSW	2	44,887,330 (GRCm39)	missense	probably damaging	1.00
R0514:Zeb2	UTSW	2	44,892,659 (GRCm39)	missense	possibly damaging	0.52
R0603:Zeb2	UTSW	2	44,907,438 (GRCm39)	missense	probably benign	0.45
R0608:Zeb2	UTSW	2	44,886,138 (GRCm39)	missense	possibly damaging	0.87
R1236:Zeb2	UTSW	2	44,884,658 (GRCm39)	missense	probably damaging	1.00
R1529:Zeb2	UTSW	2	44,887,206 (GRCm39)	missense	probably damaging	1.00
R1581:Zeb2	UTSW	2	44,887,012 (GRCm39)	missense	probably damaging	0.99
R1636:Zeb2	UTSW	2	44,892,623 (GRCm39)	missense	probably damaging	1.00
R1924:Zeb2	UTSW	2	44,892,624 (GRCm39)	missense	probably damaging	1.00
R2012:Zeb2	UTSW	2	44,887,962 (GRCm39)	missense	probably damaging	1.00
R2097:Zeb2	UTSW	2	44,887,168 (GRCm39)	missense	probably damaging	1.00
R2156:Zeb2	UTSW	2	44,878,821 (GRCm39)	missense	probably benign	0.20
R4385:Zeb2	UTSW	2	44,913,074 (GRCm39)	missense	probably damaging	1.00
R4472:Zeb2	UTSW	2	44,913,023 (GRCm39)	missense	probably damaging	1.00
R4769:Zeb2	UTSW	2	44,886,447 (GRCm39)	missense	probably damaging	1.00
R4816:Zeb2	UTSW	2	44,887,780 (GRCm39)	missense	probably damaging	0.99
R4918:Zeb2	UTSW	2	44,886,894 (GRCm39)	missense	probably damaging	1.00
R4969:Zeb2	UTSW	2	44,888,931 (GRCm39)	missense	probably damaging	1.00
R5191:Zeb2	UTSW	2	44,892,612 (GRCm39)	missense	probably benign	0.00
R5195:Zeb2	UTSW	2	44,891,647 (GRCm39)	missense	probably damaging	1.00
R5322:Zeb2	UTSW	2	44,887,107 (GRCm39)	missense	probably damaging	1.00
R5699:Zeb2	UTSW	2	44,887,800 (GRCm39)	missense	probably damaging	1.00
R5750:Zeb2	UTSW	2	44,887,530 (GRCm39)	missense	probably damaging	0.96
R5764:Zeb2	UTSW	2	44,886,931 (GRCm39)	missense	possibly damaging	0.89
R5914:Zeb2	UTSW	2	44,887,064 (GRCm39)	missense	probably benign	0.00
R5918:Zeb2	UTSW	2	45,001,271 (GRCm39)	intron	probably benign
R6037:Zeb2	UTSW	2	44,878,652 (GRCm39)	nonsense	probably null
R6037:Zeb2	UTSW	2	44,878,652 (GRCm39)	nonsense	probably null
R6302:Zeb2	UTSW	2	44,887,771 (GRCm39)	missense	probably benign	0.18
R6372:Zeb2	UTSW	2	44,892,551 (GRCm39)	missense	probably damaging	1.00
R6402:Zeb2	UTSW	2	44,886,987 (GRCm39)	missense	probably damaging	1.00
R6492:Zeb2	UTSW	2	45,000,508 (GRCm39)	intron	probably benign
R6554:Zeb2	UTSW	2	44,887,524 (GRCm39)	missense	probably damaging	1.00
R6675:Zeb2	UTSW	2	44,887,457 (GRCm39)	nonsense	probably null
R6735:Zeb2	UTSW	2	45,000,028 (GRCm39)	missense	probably null	0.99
R6870:Zeb2	UTSW	2	44,878,922 (GRCm39)	missense	probably damaging	0.98
R6925:Zeb2	UTSW	2	44,884,541 (GRCm39)	missense	probably damaging	1.00
R6963:Zeb2	UTSW	2	44,878,811 (GRCm39)	missense	probably damaging	0.97
R6972:Zeb2	UTSW	2	44,887,330 (GRCm39)	missense	probably damaging	1.00
R7144:Zeb2	UTSW	2	45,000,053 (GRCm39)	missense	possibly damaging	0.66
R7178:Zeb2	UTSW	2	44,887,006 (GRCm39)	missense	probably damaging	0.97
R7379:Zeb2	UTSW	2	44,891,829 (GRCm39)	splice site	probably null
R7419:Zeb2	UTSW	2	44,886,359 (GRCm39)	missense	probably benign	0.20
R7580:Zeb2	UTSW	2	44,884,544 (GRCm39)	missense	probably damaging	1.00
R7599:Zeb2	UTSW	2	44,884,625 (GRCm39)	missense	probably damaging	1.00
R7625:Zeb2	UTSW	2	44,892,584 (GRCm39)	missense	probably damaging	1.00
R7917:Zeb2	UTSW	2	44,886,421 (GRCm39)	missense	possibly damaging	0.50
R8132:Zeb2	UTSW	2	44,879,142 (GRCm39)	missense	probably damaging	1.00
R8412:Zeb2	UTSW	2	44,888,964 (GRCm39)	missense	probably damaging	1.00
R8413:Zeb2	UTSW	2	44,886,183 (GRCm39)	missense	probably damaging	0.99
R8417:Zeb2	UTSW	2	44,913,008 (GRCm39)	missense	probably damaging	0.99
R8750:Zeb2	UTSW	2	44,887,939 (GRCm39)	missense	probably damaging	1.00
R8865:Zeb2	UTSW	2	44,886,139 (GRCm39)	missense	probably benign	0.02
R8916:Zeb2	UTSW	2	44,886,796 (GRCm39)	missense	probably damaging	0.99
R9068:Zeb2	UTSW	2	45,000,040 (GRCm39)	missense	possibly damaging	0.95
R9094:Zeb2	UTSW	2	45,003,136 (GRCm39)	intron	probably benign
R9139:Zeb2	UTSW	2	44,878,637 (GRCm39)	missense	possibly damaging	0.52
R9187:Zeb2	UTSW	2	45,000,040 (GRCm39)	missense	possibly damaging	0.95
R9309:Zeb2	UTSW	2	44,892,575 (GRCm39)	missense	probably damaging	0.99
R9310:Zeb2	UTSW	2	44,886,988 (GRCm39)	missense	probably benign	0.01
R9337:Zeb2	UTSW	2	44,912,912 (GRCm39)	missense	probably benign	0.35
R9350:Zeb2	UTSW	2	44,887,158 (GRCm39)	missense	possibly damaging	0.79
R9371:Zeb2	UTSW	2	44,888,912 (GRCm39)	missense	probably damaging	1.00
R9389:Zeb2	UTSW	2	44,887,920 (GRCm39)	missense	probably damaging	1.00
R9509:Zeb2	UTSW	2	44,887,876 (GRCm39)	missense	possibly damaging	0.74
R9600:Zeb2	UTSW	2	44,987,180 (GRCm39)	missense	unknown
R9674:Zeb2	UTSW	2	44,891,725 (GRCm39)	missense	probably damaging	1.00
R9756:Zeb2	UTSW	2	44,887,414 (GRCm39)	missense	possibly damaging	0.74

Predicted Primers

PCR Primer
(F):5'- AAGCTCATCTGATCCAGTCCTG -3'
(R):5'- GTAGCTCGTACACTCCAAATAGC -3'

Sequencing Primer
(F):5'- CTGGGTATGGTCGTAGCCC -3'
(R):5'- CCAAATAGCTTCTCTTCCGAGG -3'

Posted On

2015-10-08